This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.52 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 116,648 (4.1%) Reads that were too short: 20,289 (0.7%) Reads written (passing filters): 96,359 (3.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 26,401,399 bp (3.1%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 116648 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1190 696.4 0 1190 7 34985 696.4 0 34985 8 40263 696.4 0 40263 9 86 696.4 0 86 10 59 696.4 0 59 11 55 696.4 0 55 12 54 696.4 0 54 13 51 696.4 0 51 14 57 696.4 0 57 15 56 696.4 0 56 16 45 696.4 0 45 17 42 696.4 0 42 18 57 696.4 0 57 19 52 696.4 0 52 20 61 696.4 0 61 21 80 696.4 0 80 22 72 696.4 0 72 23 63 696.4 0 63 24 42 696.4 0 42 25 45 696.4 0 45 26 64 696.4 0 64 27 56 696.4 0 56 28 63 696.4 0 63 29 67 696.4 0 67 30 52 696.4 0 52 31 59 696.4 0 59 32 67 696.4 0 67 33 56 696.4 0 56 34 60 696.4 0 60 35 67 696.4 0 67 36 68 696.4 0 68 37 44 696.4 0 44 38 79 696.4 0 79 39 568 696.4 0 568 40 487 696.4 0 487 41 1173 696.4 0 1173 42 382 696.4 0 382 43 57 696.4 0 57 44 47 696.4 0 47 45 43 696.4 0 43 46 57 696.4 0 57 47 50 696.4 0 50 48 34 696.4 0 34 49 70 696.4 0 70 50 54 696.4 0 54 51 52 696.4 0 52 52 56 696.4 0 56 53 62 696.4 0 62 54 50 696.4 0 50 55 43 696.4 0 43 56 36 696.4 0 36 57 58 696.4 0 58 58 56 696.4 0 56 59 51 696.4 0 51 60 79 696.4 0 79 61 47 696.4 0 47 62 45 696.4 0 45 63 42 696.4 0 42 64 63 696.4 0 63 65 1814 696.4 0 1814 66 201 696.4 0 201 67 70 696.4 0 70 68 64 696.4 0 64 69 49 696.4 0 49 70 81 696.4 0 81 71 102 696.4 0 102 72 88 696.4 0 88 73 55 696.4 0 55 74 53 696.4 0 53 75 52 696.4 0 52 76 71 696.4 0 71 77 47 696.4 0 47 78 71 696.4 0 71 79 61 696.4 0 61 80 54 696.4 0 54 81 68 696.4 0 68 82 52 696.4 0 52 83 76 696.4 0 76 84 76 696.4 0 76 85 69 696.4 0 69 86 72 696.4 0 72 87 62 696.4 0 62 88 53 696.4 0 53 89 44 696.4 0 44 90 72 696.4 0 72 91 108 696.4 0 108 92 105 696.4 0 105 93 109 696.4 0 109 94 58 696.4 0 58 95 83 696.4 0 83 96 85 696.4 0 85 97 73 696.4 0 73 98 231 696.4 0 231 99 347 696.4 0 347 100 348 696.4 0 348 101 95 696.4 0 95 102 91 696.4 0 91 103 68 696.4 0 68 104 60 696.4 0 60 105 82 696.4 0 82 106 64 696.4 0 64 107 64 696.4 0 64 108 71 696.4 0 71 109 65 696.4 0 65 110 104 696.4 0 104 111 135 696.4 0 135 112 138 696.4 0 138 113 113 696.4 0 113 114 61 696.4 0 61 115 75 696.4 0 75 116 51 696.4 0 51 117 70 696.4 0 70 118 67 696.4 0 67 119 65 696.4 0 65 120 81 696.4 0 81 121 49 696.4 0 49 122 55 696.4 0 55 123 79 696.4 0 79 124 101 696.4 0 101 125 117 696.4 0 117 126 63 696.4 0 63 127 63 696.4 0 63 128 62 696.4 0 62 129 54 696.4 0 54 130 53 696.4 0 53 131 88 696.4 0 88 132 105 696.4 0 105 133 126 696.4 0 126 134 89 696.4 0 89 135 217 696.4 0 217 136 59 696.4 0 59 137 92 696.4 0 92 138 86 696.4 0 86 139 96 696.4 0 96 140 59 696.4 0 59 141 75 696.4 0 75 142 66 696.4 0 66 143 103 696.4 0 103 144 81 696.4 0 81 145 65 696.4 0 65 146 139 696.4 0 139 147 82 696.4 0 82 148 72 696.4 0 72 149 59 696.4 0 59 150 75 696.4 0 75 151 73 696.4 0 73 152 66 696.4 0 66 153 75 696.4 0 75 154 75 696.4 0 75 155 71 696.4 0 71 156 73 696.4 0 73 157 81 696.4 0 81 158 235 696.4 0 235 159 292 696.4 0 292 160 171 696.4 0 171 161 71 696.4 0 71 162 54 696.4 0 54 163 68 696.4 0 68 164 78 696.4 0 78 165 73 696.4 0 73 166 56 696.4 0 56 167 82 696.4 0 82 168 87 696.4 0 87 169 89 696.4 0 89 170 135 696.4 0 135 171 156 696.4 0 156 172 291 696.4 0 291 173 91 696.4 0 91 174 55 696.4 0 55 175 50 696.4 0 50 176 93 696.4 0 93 177 98 696.4 0 98 178 126 696.4 0 126 179 121 696.4 0 121 180 91 696.4 0 91 181 68 696.4 0 68 182 69 696.4 0 69 183 139 696.4 0 139 184 157 696.4 0 157 185 202 696.4 0 202 186 91 696.4 0 91 187 88 696.4 0 88 188 94 696.4 0 94 189 122 696.4 0 122 190 82 696.4 0 82 191 75 696.4 0 75 192 81 696.4 0 81 193 54 696.4 0 54 194 60 696.4 0 60 195 82 696.4 0 82 196 92 696.4 0 92 197 86 696.4 0 86 198 83 696.4 0 83 199 67 696.4 0 67 200 71 696.4 0 71 201 66 696.4 0 66 202 70 696.4 0 70 203 69 696.4 0 69 204 84 696.4 0 84 205 65 696.4 0 65 206 73 696.4 0 73 207 85 696.4 0 85 208 63 696.4 0 63 209 67 696.4 0 67 210 116 696.4 0 116 211 130 696.4 0 130 212 99 696.4 0 99 213 95 696.4 0 95 214 243 696.4 0 243 215 389 696.4 0 389 216 431 696.4 0 431 217 101 696.4 0 101 218 56 696.4 0 56 219 64 696.4 0 64 220 66 696.4 0 66 221 78 696.4 0 78 222 107 696.4 0 107 223 546 696.4 0 546 224 976 696.4 0 976 225 430 696.4 0 430 226 74 696.4 0 74 227 152 696.4 0 152 228 56 696.4 0 56 229 68 696.4 0 68 230 64 696.4 0 64 231 47 696.4 0 47 232 55 696.4 0 55 233 69 696.4 0 69 234 55 696.4 0 55 235 62 696.4 0 62 236 77 696.4 0 77 237 75 696.4 0 75 238 116 696.4 0 116 239 113 696.4 0 113 240 89 696.4 0 89 241 69 696.4 0 69 242 81 696.4 0 81 243 70 696.4 0 70 244 89 696.4 0 89 245 85 696.4 0 85 246 67 696.4 0 67 247 61 696.4 0 61 248 65 696.4 0 65 249 80 696.4 0 80 250 73 696.4 0 73 251 80 696.4 0 80 252 80 696.4 0 80 253 54 696.4 0 54 254 70 696.4 0 70 255 62 696.4 0 62 256 96 696.4 0 96 257 104 696.4 0 104 258 88 696.4 0 88 259 123 696.4 0 123 260 70 696.4 0 70 261 83 696.4 0 83 262 97 696.4 0 97 263 850 696.4 0 850 264 383 696.4 0 383 265 336 696.4 0 336 266 73 696.4 0 73 267 71 696.4 0 71 268 76 696.4 0 76 269 85 696.4 0 85 270 133 696.4 0 133 271 296 696.4 0 296 272 3003 696.4 0 3003 273 2509 696.4 0 2509 274 1660 696.4 0 1660 275 87 696.4 0 87 276 98 696.4 0 98 277 127 696.4 0 127 278 144 696.4 0 144 279 92 696.4 0 92 280 65 696.4 0 65 281 68 696.4 0 68 282 94 696.4 0 94 283 171 696.4 0 171 284 251 696.4 0 251 285 292 696.4 0 292 286 182 696.4 0 182 287 86 696.4 0 86 288 106 696.4 0 106 289 104 696.4 0 104 290 52 696.4 0 52 291 75 696.4 0 75 292 53 696.4 0 53 293 50 696.4 0 50 294 65 696.4 0 65 295 61 696.4 0 61 296 64 696.4 0 64 297 88 696.4 0 88 298 185 696.4 0 185 299 295 696.4 0 295 300 292 696.4 0 292 301 145 696.4 0 145 Finished in 4.98 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 96,359 Reads with adapters: 41,662 (43.2%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 41,649 (43.2%) Total basepairs processed: 26,401,399 bp Total written (filtered): 11,323,188 bp (42.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41662 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 418 0.0 3 0 404 13 0 1 20 40614 0.0 4 39867 725 20 1 1 21 106 0.0 4 22 84 22 8 0.0 4 0 0 7 1 23 41 0.0 4 0 0 1 36 4 24 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 36 18 0.0 4 0 0 0 0 18 54 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 1 92 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 419 0.0 4 0 0 0 0 419 128 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 282 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.46 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 41,649 Reads with adapters: 3,632 (8.7%) Reads written (passing filters): 41,649 (100.0%) Total basepairs processed: 11,323,188 bp Total written (filtered): 11,284,784 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3632 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 4.1% G: 92.6% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 25 650.8 0 25 4 6 162.7 0 6 5 1 40.7 0 1 9 127 0.2 0 6 121 10 1616 0.0 1 56 1560 11 1847 0.0 1 20 1827 12 3 0.0 1 1 2 13 2 0.0 1 0 2 14 1 0.0 1 0 1 151 4 0.0 1 0 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.