This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.74 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 202,824 (7.1%) Reads that were too short: 27,871 (1.0%) Reads written (passing filters): 174,953 (6.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 37,628,551 bp (4.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 202824 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3335 696.4 0 3335 7 27963 696.4 0 27963 8 1193 696.4 0 1193 9 30571 696.4 0 30571 10 182 696.4 0 182 11 151 696.4 0 151 12 96 696.4 0 96 13 108 696.4 0 108 14 117 696.4 0 117 15 97 696.4 0 97 16 86 696.4 0 86 17 116 696.4 0 116 18 129 696.4 0 129 19 118 696.4 0 118 20 152 696.4 0 152 21 321 696.4 0 321 22 342 696.4 0 342 23 436 696.4 0 436 24 866 696.4 0 866 25 1554 696.4 0 1554 26 1996 696.4 0 1996 27 114 696.4 0 114 28 83 696.4 0 83 29 77 696.4 0 77 30 75 696.4 0 75 31 80 696.4 0 80 32 98 696.4 0 98 33 113 696.4 0 113 34 77 696.4 0 77 35 83 696.4 0 83 36 108 696.4 0 108 37 98 696.4 0 98 38 64 696.4 0 64 39 69 696.4 0 69 40 94 696.4 0 94 41 106 696.4 0 106 42 85 696.4 0 85 43 63 696.4 0 63 44 71 696.4 0 71 45 109 696.4 0 109 46 102 696.4 0 102 47 93 696.4 0 93 48 114 696.4 0 114 49 98 696.4 0 98 50 94 696.4 0 94 51 93 696.4 0 93 52 94 696.4 0 94 53 103 696.4 0 103 54 127 696.4 0 127 55 127 696.4 0 127 56 125 696.4 0 125 57 118 696.4 0 118 58 99 696.4 0 99 59 114 696.4 0 114 60 128 696.4 0 128 61 883 696.4 0 883 62 668 696.4 0 668 63 737 696.4 0 737 64 90 696.4 0 90 65 98 696.4 0 98 66 111 696.4 0 111 67 87 696.4 0 87 68 115 696.4 0 115 69 264 696.4 0 264 70 324 696.4 0 324 71 404 696.4 0 404 72 160 696.4 0 160 73 283 696.4 0 283 74 140 696.4 0 140 75 136 696.4 0 136 76 149 696.4 0 149 77 128 696.4 0 128 78 155 696.4 0 155 79 179 696.4 0 179 80 304 696.4 0 304 81 173 696.4 0 173 82 441 696.4 0 441 83 2468 696.4 0 2468 84 1694 696.4 0 1694 85 1657 696.4 0 1657 86 161 696.4 0 161 87 102 696.4 0 102 88 114 696.4 0 114 89 223 696.4 0 223 90 138 696.4 0 138 91 86 696.4 0 86 92 150 696.4 0 150 93 105 696.4 0 105 94 82 696.4 0 82 95 139 696.4 0 139 96 122 696.4 0 122 97 136 696.4 0 136 98 160 696.4 0 160 99 214 696.4 0 214 100 103 696.4 0 103 101 154 696.4 0 154 102 198 696.4 0 198 103 136 696.4 0 136 104 129 696.4 0 129 105 217 696.4 0 217 106 225 696.4 0 225 107 230 696.4 0 230 108 153 696.4 0 153 109 211 696.4 0 211 110 305 696.4 0 305 111 497 696.4 0 497 112 6264 696.4 0 6264 113 3778 696.4 0 3778 114 6136 696.4 0 6136 115 241 696.4 0 241 116 142 696.4 0 142 117 195 696.4 0 195 118 259 696.4 0 259 119 423 696.4 0 423 120 4535 696.4 0 4535 121 2277 696.4 0 2277 122 2576 696.4 0 2576 123 252 696.4 0 252 124 258 696.4 0 258 125 760 696.4 0 760 126 248 696.4 0 248 127 319 696.4 0 319 128 146 696.4 0 146 129 137 696.4 0 137 130 221 696.4 0 221 131 162 696.4 0 162 132 160 696.4 0 160 133 152 696.4 0 152 134 233 696.4 0 233 135 162 696.4 0 162 136 185 696.4 0 185 137 177 696.4 0 177 138 234 696.4 0 234 139 206 696.4 0 206 140 183 696.4 0 183 141 271 696.4 0 271 142 656 696.4 0 656 143 480 696.4 0 480 144 668 696.4 0 668 145 328 696.4 0 328 146 547 696.4 0 547 147 3346 696.4 0 3346 148 3384 696.4 0 3384 149 3689 696.4 0 3689 150 2624 696.4 0 2624 151 545 696.4 0 545 152 594 696.4 0 594 153 291 696.4 0 291 154 188 696.4 0 188 155 236 696.4 0 236 156 181 696.4 0 181 157 345 696.4 0 345 158 217 696.4 0 217 159 185 696.4 0 185 160 189 696.4 0 189 161 262 696.4 0 262 162 242 696.4 0 242 163 442 696.4 0 442 164 402 696.4 0 402 165 748 696.4 0 748 166 1399 696.4 0 1399 167 1277 696.4 0 1277 168 1194 696.4 0 1194 169 802 696.4 0 802 170 582 696.4 0 582 171 2533 696.4 0 2533 172 2032 696.4 0 2032 173 2749 696.4 0 2749 174 937 696.4 0 937 175 385 696.4 0 385 176 311 696.4 0 311 177 246 696.4 0 246 178 204 696.4 0 204 179 167 696.4 0 167 180 173 696.4 0 173 181 234 696.4 0 234 182 305 696.4 0 305 183 1783 696.4 0 1783 184 1239 696.4 0 1239 185 742 696.4 0 742 186 417 696.4 0 417 187 472 696.4 0 472 188 472 696.4 0 472 189 528 696.4 0 528 190 460 696.4 0 460 191 2338 696.4 0 2338 192 2644 696.4 0 2644 193 3216 696.4 0 3216 194 1303 696.4 0 1303 195 357 696.4 0 357 196 326 696.4 0 326 197 237 696.4 0 237 198 189 696.4 0 189 199 259 696.4 0 259 200 427 696.4 0 427 201 370 696.4 0 370 202 282 696.4 0 282 203 178 696.4 0 178 204 136 696.4 0 136 205 166 696.4 0 166 206 189 696.4 0 189 207 268 696.4 0 268 208 234 696.4 0 234 209 192 696.4 0 192 210 150 696.4 0 150 211 129 696.4 0 129 212 150 696.4 0 150 213 161 696.4 0 161 214 159 696.4 0 159 215 336 696.4 0 336 216 260 696.4 0 260 217 231 696.4 0 231 218 308 696.4 0 308 219 207 696.4 0 207 220 213 696.4 0 213 221 116 696.4 0 116 222 139 696.4 0 139 223 150 696.4 0 150 224 142 696.4 0 142 225 259 696.4 0 259 226 718 696.4 0 718 227 432 696.4 0 432 228 573 696.4 0 573 229 441 696.4 0 441 230 339 696.4 0 339 231 132 696.4 0 132 232 143 696.4 0 143 233 156 696.4 0 156 234 126 696.4 0 126 235 144 696.4 0 144 236 119 696.4 0 119 237 162 696.4 0 162 238 136 696.4 0 136 239 156 696.4 0 156 240 145 696.4 0 145 241 128 696.4 0 128 242 144 696.4 0 144 243 190 696.4 0 190 244 153 696.4 0 153 245 248 696.4 0 248 246 408 696.4 0 408 247 343 696.4 0 343 248 213 696.4 0 213 249 123 696.4 0 123 250 134 696.4 0 134 251 382 696.4 0 382 252 267 696.4 0 267 253 299 696.4 0 299 254 212 696.4 0 212 255 200 696.4 0 200 256 242 696.4 0 242 257 266 696.4 0 266 258 170 696.4 0 170 259 176 696.4 0 176 260 139 696.4 0 139 261 159 696.4 0 159 262 191 696.4 0 191 263 149 696.4 0 149 264 198 696.4 0 198 265 224 696.4 0 224 266 364 696.4 0 364 267 3403 696.4 0 3403 268 1681 696.4 0 1681 269 1700 696.4 0 1700 270 191 696.4 0 191 271 133 696.4 0 133 272 117 696.4 0 117 273 137 696.4 0 137 274 144 696.4 0 144 275 192 696.4 0 192 276 165 696.4 0 165 277 451 696.4 0 451 278 253 696.4 0 253 279 365 696.4 0 365 280 520 696.4 0 520 281 227 696.4 0 227 282 653 696.4 0 653 283 161 696.4 0 161 284 119 696.4 0 119 285 110 696.4 0 110 286 146 696.4 0 146 287 124 696.4 0 124 288 154 696.4 0 154 289 176 696.4 0 176 290 147 696.4 0 147 291 117 696.4 0 117 292 148 696.4 0 148 293 164 696.4 0 164 294 188 696.4 0 188 295 167 696.4 0 167 296 166 696.4 0 166 297 215 696.4 0 215 298 358 696.4 0 358 299 235 696.4 0 235 300 138 696.4 0 138 301 107 696.4 0 107 Finished in 7.23 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 174,953 Reads with adapters: 36,287 (20.7%) Reads that were too short: 739 (0.4%) Reads written (passing filters): 35,548 (20.3%) Total basepairs processed: 37,628,551 bp Total written (filtered): 9,541,454 bp (25.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36287 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 19 0.0 3 0 0 14 5 16 316 0.0 3 0 278 25 13 17 31350 0.0 3 29272 1700 290 88 18 126 0.0 3 11 77 12 26 19 70 0.0 3 2 10 36 22 20 2087 0.0 3 1918 111 20 38 21 23 0.0 3 1 8 0 14 22 8 0.0 3 0 0 3 5 23 10 0.0 3 0 0 0 10 24 454 0.0 3 0 0 7 447 25 1 0.0 3 0 0 0 1 26 36 0.0 3 0 0 0 36 27 95 0.0 3 0 0 2 93 28 21 0.0 3 0 0 0 21 29 4 0.0 3 0 0 0 4 30 8 0.0 3 0 0 0 8 31 11 0.0 3 0 0 0 11 33 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 5 0.0 3 0 0 1 4 41 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 46 4 0.0 3 0 0 0 4 48 9 0.0 3 0 0 0 9 49 5 0.0 3 0 0 0 5 50 27 0.0 3 0 0 1 26 51 81 0.0 3 0 0 1 80 52 7 0.0 3 0 0 0 7 53 8 0.0 3 0 0 0 8 54 17 0.0 3 0 0 0 17 55 10 0.0 3 0 0 1 9 56 1 0.0 3 0 0 0 1 57 6 0.0 3 0 0 0 6 58 3 0.0 3 0 0 1 2 59 25 0.0 3 0 0 1 24 60 16 0.0 3 0 0 0 16 61 6 0.0 3 0 0 0 6 62 14 0.0 3 0 0 0 14 63 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 85 0.0 3 0 0 3 82 67 33 0.0 3 0 0 2 31 68 73 0.0 3 0 1 0 72 69 23 0.0 3 0 0 1 22 70 17 0.0 3 0 0 0 17 71 20 0.0 3 0 0 0 20 72 3 0.0 3 0 0 0 3 73 2 0.0 3 0 0 0 2 74 3 0.0 3 0 0 0 3 75 22 0.0 3 0 0 1 21 76 10 0.0 3 0 0 0 10 77 9 0.0 3 0 0 0 9 78 2 0.0 3 0 0 0 2 79 30 0.0 3 0 0 1 29 80 2 0.0 3 0 0 0 2 82 9 0.0 3 0 0 0 9 83 1 0.0 3 0 0 0 1 84 28 0.0 3 0 0 0 28 85 2 0.0 3 0 0 0 2 86 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 93 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 14 0.0 3 0 0 0 14 99 12 0.0 3 0 0 1 11 100 4 0.0 3 0 0 0 4 101 17 0.0 3 0 0 1 16 102 18 0.0 3 0 0 0 18 103 4 0.0 3 0 0 0 4 105 17 0.0 3 0 0 0 17 106 1 0.0 3 0 0 0 1 107 7 0.0 3 0 0 0 7 108 43 0.0 3 0 0 0 43 109 51 0.0 3 0 0 0 51 110 32 0.0 3 0 0 1 31 111 11 0.0 3 0 0 1 10 112 42 0.0 3 0 0 0 42 113 16 0.0 3 0 0 2 14 114 10 0.0 3 0 0 0 10 115 16 0.0 3 0 0 1 15 116 4 0.0 3 0 0 0 4 117 8 0.0 3 0 0 0 8 118 1 0.0 3 0 0 1 119 4 0.0 3 0 0 0 4 120 6 0.0 3 0 0 1 5 121 2 0.0 3 0 0 1 1 122 2 0.0 3 0 0 0 2 123 4 0.0 3 0 0 0 4 124 5 0.0 3 0 0 0 5 125 7 0.0 3 0 0 0 7 126 9 0.0 3 0 0 0 9 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 5 0.0 3 0 0 0 5 130 15 0.0 3 0 0 0 15 131 3 0.0 3 0 0 0 3 132 6 0.0 3 0 0 0 6 133 3 0.0 3 0 0 0 3 134 14 0.0 3 0 0 0 14 135 2 0.0 3 0 0 0 2 136 52 0.0 3 0 0 1 51 137 14 0.0 3 0 0 0 14 138 9 0.0 3 0 0 0 9 139 43 0.0 3 0 0 0 43 140 5 0.0 3 0 0 1 4 141 1 0.0 3 0 0 0 1 142 5 0.0 3 0 0 0 5 143 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 145 4 0.0 3 0 0 0 4 146 2 0.0 3 0 0 0 2 147 32 0.0 3 0 0 2 30 148 9 0.0 3 0 0 0 9 149 2 0.0 3 0 0 0 2 150 19 0.0 3 0 0 1 18 151 6 0.0 3 0 0 0 6 152 3 0.0 3 0 0 0 3 153 4 0.0 3 0 0 0 4 154 10 0.0 3 0 0 1 9 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 1 1 159 4 0.0 3 0 0 0 4 160 4 0.0 3 0 0 0 4 161 2 0.0 3 0 0 0 2 162 21 0.0 3 0 0 1 20 163 8 0.0 3 0 0 0 8 164 5 0.0 3 0 0 0 5 165 4 0.0 3 0 0 1 3 166 6 0.0 3 0 0 2 4 167 50 0.0 3 0 0 0 50 168 20 0.0 3 0 0 1 19 169 6 0.0 3 0 0 0 6 170 18 0.0 3 0 0 1 17 171 11 0.0 3 0 0 0 11 172 2 0.0 3 0 0 0 2 173 10 0.0 3 0 0 0 10 174 4 0.0 3 0 0 0 4 176 3 0.0 3 0 0 0 3 177 3 0.0 3 0 0 0 3 178 3 0.0 3 0 0 0 3 179 5 0.0 3 0 0 0 5 180 4 0.0 3 0 0 0 4 181 22 0.0 3 0 0 0 22 182 2 0.0 3 0 0 0 2 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 4 0.0 3 0 0 0 4 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 3 0.0 3 0 0 1 2 202 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 4 0.0 3 0 0 0 4 210 4 0.0 3 0 0 0 4 211 2 0.0 3 0 0 0 2 213 3 0.0 3 0 0 0 3 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 223 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 230 3 0.0 3 0 0 0 3 231 1 0.0 3 0 0 0 1 232 2 0.0 3 0 0 0 2 235 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 259 2 0.0 3 0 0 0 2 261 2 0.0 3 0 1 0 1 262 2 0.0 3 0 0 1 1 263 5 0.0 3 0 0 0 5 265 2 0.0 3 0 0 0 2 267 3 0.0 3 0 0 1 2 268 6 0.0 3 0 0 0 6 269 6 0.0 3 0 0 0 6 270 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 294 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.25 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 35,548 Reads with adapters: 1,548 (4.4%) Reads written (passing filters): 35,548 (100.0%) Total basepairs processed: 9,541,454 bp Total written (filtered): 9,536,748 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1548 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.4% C: 12.2% G: 25.5% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1488 555.4 0 1488 4 58 138.9 0 58 5 2 34.7 0 2