This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.50 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 174,309 (6.1%) Reads that were too short: 25,958 (0.9%) Reads written (passing filters): 148,351 (5.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 33,375,653 bp (3.9%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 174309 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2756 696.4 0 2756 7 33564 696.4 0 33564 8 748 696.4 0 748 9 27681 696.4 0 27681 10 126 696.4 0 126 11 119 696.4 0 119 12 108 696.4 0 108 13 113 696.4 0 113 14 114 696.4 0 114 15 100 696.4 0 100 16 112 696.4 0 112 17 129 696.4 0 129 18 113 696.4 0 113 19 117 696.4 0 117 20 111 696.4 0 111 21 186 696.4 0 186 22 178 696.4 0 178 23 172 696.4 0 172 24 311 696.4 0 311 25 467 696.4 0 467 26 841 696.4 0 841 27 106 696.4 0 106 28 118 696.4 0 118 29 115 696.4 0 115 30 92 696.4 0 92 31 76 696.4 0 76 32 125 696.4 0 125 33 110 696.4 0 110 34 107 696.4 0 107 35 106 696.4 0 106 36 120 696.4 0 120 37 87 696.4 0 87 38 119 696.4 0 119 39 116 696.4 0 116 40 114 696.4 0 114 41 126 696.4 0 126 42 92 696.4 0 92 43 109 696.4 0 109 44 120 696.4 0 120 45 102 696.4 0 102 46 128 696.4 0 128 47 106 696.4 0 106 48 100 696.4 0 100 49 114 696.4 0 114 50 96 696.4 0 96 51 204 696.4 0 204 52 101 696.4 0 101 53 115 696.4 0 115 54 127 696.4 0 127 55 97 696.4 0 97 56 109 696.4 0 109 57 104 696.4 0 104 58 129 696.4 0 129 59 147 696.4 0 147 60 126 696.4 0 126 61 530 696.4 0 530 62 354 696.4 0 354 63 303 696.4 0 303 64 102 696.4 0 102 65 136 696.4 0 136 66 131 696.4 0 131 67 116 696.4 0 116 68 99 696.4 0 99 69 238 696.4 0 238 70 219 696.4 0 219 71 185 696.4 0 185 72 117 696.4 0 117 73 93 696.4 0 93 74 108 696.4 0 108 75 113 696.4 0 113 76 142 696.4 0 142 77 166 696.4 0 166 78 177 696.4 0 177 79 169 696.4 0 169 80 211 696.4 0 211 81 171 696.4 0 171 82 251 696.4 0 251 83 1054 696.4 0 1054 84 677 696.4 0 677 85 802 696.4 0 802 86 140 696.4 0 140 87 155 696.4 0 155 88 168 696.4 0 168 89 150 696.4 0 150 90 151 696.4 0 151 91 136 696.4 0 136 92 155 696.4 0 155 93 127 696.4 0 127 94 104 696.4 0 104 95 136 696.4 0 136 96 140 696.4 0 140 97 148 696.4 0 148 98 186 696.4 0 186 99 276 696.4 0 276 100 144 696.4 0 144 101 172 696.4 0 172 102 253 696.4 0 253 103 144 696.4 0 144 104 149 696.4 0 149 105 201 696.4 0 201 106 187 696.4 0 187 107 256 696.4 0 256 108 188 696.4 0 188 109 216 696.4 0 216 110 295 696.4 0 295 111 410 696.4 0 410 112 5966 696.4 0 5966 113 3738 696.4 0 3738 114 6267 696.4 0 6267 115 210 696.4 0 210 116 169 696.4 0 169 117 173 696.4 0 173 118 240 696.4 0 240 119 313 696.4 0 313 120 4767 696.4 0 4767 121 2317 696.4 0 2317 122 1931 696.4 0 1931 123 186 696.4 0 186 124 226 696.4 0 226 125 613 696.4 0 613 126 310 696.4 0 310 127 341 696.4 0 341 128 135 696.4 0 135 129 154 696.4 0 154 130 179 696.4 0 179 131 152 696.4 0 152 132 140 696.4 0 140 133 126 696.4 0 126 134 170 696.4 0 170 135 136 696.4 0 136 136 148 696.4 0 148 137 156 696.4 0 156 138 194 696.4 0 194 139 169 696.4 0 169 140 186 696.4 0 186 141 175 696.4 0 175 142 433 696.4 0 433 143 368 696.4 0 368 144 416 696.4 0 416 145 192 696.4 0 192 146 231 696.4 0 231 147 1287 696.4 0 1287 148 1355 696.4 0 1355 149 2114 696.4 0 2114 150 1146 696.4 0 1146 151 270 696.4 0 270 152 331 696.4 0 331 153 177 696.4 0 177 154 160 696.4 0 160 155 186 696.4 0 186 156 152 696.4 0 152 157 223 696.4 0 223 158 192 696.4 0 192 159 158 696.4 0 158 160 156 696.4 0 156 161 185 696.4 0 185 162 193 696.4 0 193 163 199 696.4 0 199 164 202 696.4 0 202 165 326 696.4 0 326 166 750 696.4 0 750 167 619 696.4 0 619 168 678 696.4 0 678 169 430 696.4 0 430 170 355 696.4 0 355 171 1582 696.4 0 1582 172 1680 696.4 0 1680 173 1958 696.4 0 1958 174 682 696.4 0 682 175 304 696.4 0 304 176 254 696.4 0 254 177 258 696.4 0 258 178 180 696.4 0 180 179 166 696.4 0 166 180 185 696.4 0 185 181 203 696.4 0 203 182 230 696.4 0 230 183 2416 696.4 0 2416 184 1456 696.4 0 1456 185 861 696.4 0 861 186 276 696.4 0 276 187 274 696.4 0 274 188 304 696.4 0 304 189 293 696.4 0 293 190 269 696.4 0 269 191 1287 696.4 0 1287 192 1744 696.4 0 1744 193 1853 696.4 0 1853 194 765 696.4 0 765 195 219 696.4 0 219 196 296 696.4 0 296 197 209 696.4 0 209 198 205 696.4 0 205 199 274 696.4 0 274 200 424 696.4 0 424 201 293 696.4 0 293 202 228 696.4 0 228 203 177 696.4 0 177 204 132 696.4 0 132 205 142 696.4 0 142 206 160 696.4 0 160 207 177 696.4 0 177 208 172 696.4 0 172 209 168 696.4 0 168 210 157 696.4 0 157 211 131 696.4 0 131 212 131 696.4 0 131 213 155 696.4 0 155 214 138 696.4 0 138 215 352 696.4 0 352 216 275 696.4 0 275 217 231 696.4 0 231 218 227 696.4 0 227 219 194 696.4 0 194 220 163 696.4 0 163 221 144 696.4 0 144 222 120 696.4 0 120 223 125 696.4 0 125 224 143 696.4 0 143 225 199 696.4 0 199 226 488 696.4 0 488 227 336 696.4 0 336 228 492 696.4 0 492 229 366 696.4 0 366 230 326 696.4 0 326 231 116 696.4 0 116 232 129 696.4 0 129 233 139 696.4 0 139 234 148 696.4 0 148 235 147 696.4 0 147 236 135 696.4 0 135 237 186 696.4 0 186 238 148 696.4 0 148 239 135 696.4 0 135 240 148 696.4 0 148 241 149 696.4 0 149 242 124 696.4 0 124 243 163 696.4 0 163 244 149 696.4 0 149 245 194 696.4 0 194 246 187 696.4 0 187 247 202 696.4 0 202 248 123 696.4 0 123 249 117 696.4 0 117 250 134 696.4 0 134 251 299 696.4 0 299 252 228 696.4 0 228 253 251 696.4 0 251 254 169 696.4 0 169 255 160 696.4 0 160 256 200 696.4 0 200 257 211 696.4 0 211 258 159 696.4 0 159 259 156 696.4 0 156 260 129 696.4 0 129 261 138 696.4 0 138 262 155 696.4 0 155 263 154 696.4 0 154 264 184 696.4 0 184 265 207 696.4 0 207 266 324 696.4 0 324 267 3957 696.4 0 3957 268 1914 696.4 0 1914 269 2060 696.4 0 2060 270 152 696.4 0 152 271 133 696.4 0 133 272 95 696.4 0 95 273 88 696.4 0 88 274 113 696.4 0 113 275 139 696.4 0 139 276 128 696.4 0 128 277 291 696.4 0 291 278 204 696.4 0 204 279 280 696.4 0 280 280 555 696.4 0 555 281 153 696.4 0 153 282 778 696.4 0 778 283 131 696.4 0 131 284 114 696.4 0 114 285 93 696.4 0 93 286 133 696.4 0 133 287 105 696.4 0 105 288 116 696.4 0 116 289 152 696.4 0 152 290 137 696.4 0 137 291 141 696.4 0 141 292 119 696.4 0 119 293 126 696.4 0 126 294 156 696.4 0 156 295 148 696.4 0 148 296 124 696.4 0 124 297 142 696.4 0 142 298 240 696.4 0 240 299 149 696.4 0 149 300 122 696.4 0 122 301 124 696.4 0 124 Finished in 6.94 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 148,351 Reads with adapters: 35,296 (23.8%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 35,245 (23.8%) Total basepairs processed: 33,375,653 bp Total written (filtered): 9,500,231 bp (28.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35296 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 6 0.0 3 0 0 6 19 274 0.0 3 0 267 7 20 33410 0.0 4 32788 594 22 1 5 21 87 0.0 4 10 77 22 7 0.0 4 1 0 5 0 1 23 16 0.0 4 2 0 0 12 2 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 49 2 0.0 4 0 0 0 0 2 50 1 0.0 4 0 0 0 0 1 51 58 0.0 4 0 0 0 0 58 54 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 1 1 73 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 110 19 0.0 4 0 0 0 0 19 113 2 0.0 4 0 0 0 0 2 119 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 16 0.0 4 0 0 0 0 16 127 1324 0.0 4 0 0 0 0 1324 128 2 0.0 4 0 0 0 0 2 130 16 0.0 4 0 0 0 0 16 140 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 158 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 177 5 0.0 4 0 0 0 0 5 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 4 0.0 4 0 0 0 0 4 211 1 0.0 4 1 228 3 0.0 4 0 0 0 0 3 229 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 247 1 0.0 4 1 264 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.21 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 35,245 Reads with adapters: 1,047 (3.0%) Reads written (passing filters): 35,245 (100.0%) Total basepairs processed: 9,500,231 bp Total written (filtered): 9,488,788 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1047 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.5% C: 4.8% G: 89.1% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 45 550.7 0 45 4 6 137.7 0 6 5 1 34.4 0 1 8 1 0.5 0 1 9 5 0.1 0 0 5 10 21 0.0 1 1 20 11 910 0.0 1 7 903 12 56 0.0 1 4 52 124 1 0.0 1 1 210 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.