This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.36 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 127,956 (4.5%) Reads that were too short: 40,422 (1.4%) Reads written (passing filters): 87,534 (3.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,331,910 bp (2.7%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 127956 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 416 696.4 0 416 7 1072 696.4 0 1072 8 36192 696.4 0 36192 9 27867 696.4 0 27867 10 95 696.4 0 95 11 73 696.4 0 73 12 83 696.4 0 83 13 67 696.4 0 67 14 93 696.4 0 93 15 96 696.4 0 96 16 89 696.4 0 89 17 108 696.4 0 108 18 85 696.4 0 85 19 98 696.4 0 98 20 98 696.4 0 98 21 100 696.4 0 100 22 85 696.4 0 85 23 105 696.4 0 105 24 87 696.4 0 87 25 92 696.4 0 92 26 93 696.4 0 93 27 88 696.4 0 88 28 69 696.4 0 69 29 97 696.4 0 97 30 105 696.4 0 105 31 70 696.4 0 70 32 97 696.4 0 97 33 67 696.4 0 67 34 100 696.4 0 100 35 74 696.4 0 74 36 83 696.4 0 83 37 83 696.4 0 83 38 63 696.4 0 63 39 79 696.4 0 79 40 61 696.4 0 61 41 77 696.4 0 77 42 70 696.4 0 70 43 84 696.4 0 84 44 106 696.4 0 106 45 125 696.4 0 125 46 120 696.4 0 120 47 95 696.4 0 95 48 84 696.4 0 84 49 78 696.4 0 78 50 80 696.4 0 80 51 85 696.4 0 85 52 82 696.4 0 82 53 113 696.4 0 113 54 104 696.4 0 104 55 97 696.4 0 97 56 138 696.4 0 138 57 79 696.4 0 79 58 102 696.4 0 102 59 124 696.4 0 124 60 127 696.4 0 127 61 285 696.4 0 285 62 70 696.4 0 70 63 72 696.4 0 72 64 120 696.4 0 120 65 95 696.4 0 95 66 113 696.4 0 113 67 90 696.4 0 90 68 66 696.4 0 66 69 80 696.4 0 80 70 175 696.4 0 175 71 240 696.4 0 240 72 177 696.4 0 177 73 111 696.4 0 111 74 87 696.4 0 87 75 116 696.4 0 116 76 200 696.4 0 200 77 186 696.4 0 186 78 251 696.4 0 251 79 98 696.4 0 98 80 109 696.4 0 109 81 146 696.4 0 146 82 107 696.4 0 107 83 121 696.4 0 121 84 121 696.4 0 121 85 132 696.4 0 132 86 118 696.4 0 118 87 97 696.4 0 97 88 100 696.4 0 100 89 93 696.4 0 93 90 135 696.4 0 135 91 116 696.4 0 116 92 126 696.4 0 126 93 110 696.4 0 110 94 104 696.4 0 104 95 125 696.4 0 125 96 89 696.4 0 89 97 79 696.4 0 79 98 99 696.4 0 99 99 85 696.4 0 85 100 96 696.4 0 96 101 78 696.4 0 78 102 83 696.4 0 83 103 88 696.4 0 88 104 89 696.4 0 89 105 100 696.4 0 100 106 123 696.4 0 123 107 72 696.4 0 72 108 86 696.4 0 86 109 98 696.4 0 98 110 123 696.4 0 123 111 121 696.4 0 121 112 190 696.4 0 190 113 114 696.4 0 114 114 99 696.4 0 99 115 104 696.4 0 104 116 85 696.4 0 85 117 112 696.4 0 112 118 131 696.4 0 131 119 115 696.4 0 115 120 104 696.4 0 104 121 88 696.4 0 88 122 97 696.4 0 97 123 81 696.4 0 81 124 101 696.4 0 101 125 82 696.4 0 82 126 105 696.4 0 105 127 103 696.4 0 103 128 112 696.4 0 112 129 131 696.4 0 131 130 182 696.4 0 182 131 182 696.4 0 182 132 182 696.4 0 182 133 137 696.4 0 137 134 164 696.4 0 164 135 103 696.4 0 103 136 106 696.4 0 106 137 101 696.4 0 101 138 145 696.4 0 145 139 203 696.4 0 203 140 176 696.4 0 176 141 117 696.4 0 117 142 120 696.4 0 120 143 118 696.4 0 118 144 206 696.4 0 206 145 197 696.4 0 197 146 121 696.4 0 121 147 97 696.4 0 97 148 95 696.4 0 95 149 111 696.4 0 111 150 91 696.4 0 91 151 181 696.4 0 181 152 157 696.4 0 157 153 124 696.4 0 124 154 113 696.4 0 113 155 100 696.4 0 100 156 160 696.4 0 160 157 147 696.4 0 147 158 113 696.4 0 113 159 110 696.4 0 110 160 88 696.4 0 88 161 107 696.4 0 107 162 84 696.4 0 84 163 92 696.4 0 92 164 107 696.4 0 107 165 116 696.4 0 116 166 98 696.4 0 98 167 86 696.4 0 86 168 97 696.4 0 97 169 96 696.4 0 96 170 81 696.4 0 81 171 109 696.4 0 109 172 78 696.4 0 78 173 103 696.4 0 103 174 91 696.4 0 91 175 85 696.4 0 85 176 103 696.4 0 103 177 71 696.4 0 71 178 86 696.4 0 86 179 86 696.4 0 86 180 75 696.4 0 75 181 79 696.4 0 79 182 90 696.4 0 90 183 107 696.4 0 107 184 87 696.4 0 87 185 103 696.4 0 103 186 108 696.4 0 108 187 103 696.4 0 103 188 105 696.4 0 105 189 104 696.4 0 104 190 120 696.4 0 120 191 248 696.4 0 248 192 238 696.4 0 238 193 187 696.4 0 187 194 153 696.4 0 153 195 141 696.4 0 141 196 129 696.4 0 129 197 382 696.4 0 382 198 331 696.4 0 331 199 278 696.4 0 278 200 98 696.4 0 98 201 90 696.4 0 90 202 119 696.4 0 119 203 74 696.4 0 74 204 135 696.4 0 135 205 92 696.4 0 92 206 108 696.4 0 108 207 74 696.4 0 74 208 85 696.4 0 85 209 78 696.4 0 78 210 69 696.4 0 69 211 66 696.4 0 66 212 97 696.4 0 97 213 108 696.4 0 108 214 106 696.4 0 106 215 122 696.4 0 122 216 96 696.4 0 96 217 101 696.4 0 101 218 112 696.4 0 112 219 92 696.4 0 92 220 99 696.4 0 99 221 124 696.4 0 124 222 142 696.4 0 142 223 244 696.4 0 244 224 202 696.4 0 202 225 141 696.4 0 141 226 82 696.4 0 82 227 112 696.4 0 112 228 79 696.4 0 79 229 98 696.4 0 98 230 104 696.4 0 104 231 112 696.4 0 112 232 107 696.4 0 107 233 150 696.4 0 150 234 166 696.4 0 166 235 160 696.4 0 160 236 123 696.4 0 123 237 146 696.4 0 146 238 152 696.4 0 152 239 161 696.4 0 161 240 150 696.4 0 150 241 138 696.4 0 138 242 131 696.4 0 131 243 149 696.4 0 149 244 120 696.4 0 120 245 178 696.4 0 178 246 219 696.4 0 219 247 316 696.4 0 316 248 140 696.4 0 140 249 94 696.4 0 94 250 88 696.4 0 88 251 118 696.4 0 118 252 119 696.4 0 119 253 115 696.4 0 115 254 221 696.4 0 221 255 227 696.4 0 227 256 283 696.4 0 283 257 187 696.4 0 187 258 198 696.4 0 198 259 168 696.4 0 168 260 182 696.4 0 182 261 202 696.4 0 202 262 236 696.4 0 236 263 212 696.4 0 212 264 255 696.4 0 255 265 963 696.4 0 963 266 947 696.4 0 947 267 1296 696.4 0 1296 268 253 696.4 0 253 269 93 696.4 0 93 270 126 696.4 0 126 271 146 696.4 0 146 272 240 696.4 0 240 273 279 696.4 0 279 274 233 696.4 0 233 275 165 696.4 0 165 276 92 696.4 0 92 277 121 696.4 0 121 278 188 696.4 0 188 279 168 696.4 0 168 280 202 696.4 0 202 281 276 696.4 0 276 282 219 696.4 0 219 283 174 696.4 0 174 284 181 696.4 0 181 285 203 696.4 0 203 286 235 696.4 0 235 287 213 696.4 0 213 288 311 696.4 0 311 289 311 696.4 0 311 290 464 696.4 0 464 291 773 696.4 0 773 292 712 696.4 0 712 293 702 696.4 0 702 294 710 696.4 0 710 295 714 696.4 0 714 296 687 696.4 0 687 297 1292 696.4 0 1292 298 5417 696.4 0 5417 299 4188 696.4 0 4188 300 6492 696.4 0 6492 301 1752 696.4 0 1752 Finished in 4.12 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 87,534 Reads with adapters: 35,268 (40.3%) Reads that were too short: 84 (0.1%) Reads written (passing filters): 35,184 (40.2%) Total basepairs processed: 23,331,910 bp Total written (filtered): 9,704,854 bp (41.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 35268 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 20 0.0 3 0 0 10 10 16 398 0.0 3 0 351 29 18 17 34380 0.0 3 32269 1793 250 68 18 174 0.0 3 33 108 13 20 19 106 0.0 3 0 0 64 42 20 52 0.0 3 1 0 0 51 21 3 0.0 3 0 0 0 3 22 1 0.0 3 1 24 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 1 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 11 0.0 3 0 0 0 11 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 149 7 0.0 3 0 0 0 7 153 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 167 3 0.0 3 0 0 0 3 183 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 189 26 0.0 3 0 0 0 26 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 4 0.0 3 0 0 0 4 281 2 0.0 3 0 0 0 2 282 4 0.0 3 0 0 0 4 283 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.21 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 35,184 Reads with adapters: 850 (2.4%) Reads written (passing filters): 35,184 (100.0%) Total basepairs processed: 9,704,854 bp Total written (filtered): 9,702,040 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 850 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.3% C: 10.2% G: 40.8% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 599 549.8 0 599 4 238 137.4 0 238 5 13 34.4 0 13