This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.86 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 141,957 (5.0%) Reads that were too short: 56,474 (2.0%) Reads written (passing filters): 85,483 (3.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,202,462 bp (2.7%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 141957 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 311 696.4 0 311 7 859 696.4 0 859 8 32621 696.4 0 32621 9 34247 696.4 0 34247 10 98 696.4 0 98 11 55 696.4 0 55 12 92 696.4 0 92 13 62 696.4 0 62 14 80 696.4 0 80 15 91 696.4 0 91 16 86 696.4 0 86 17 75 696.4 0 75 18 72 696.4 0 72 19 66 696.4 0 66 20 72 696.4 0 72 21 68 696.4 0 68 22 82 696.4 0 82 23 94 696.4 0 94 24 82 696.4 0 82 25 70 696.4 0 70 26 65 696.4 0 65 27 64 696.4 0 64 28 76 696.4 0 76 29 70 696.4 0 70 30 59 696.4 0 59 31 50 696.4 0 50 32 53 696.4 0 53 33 57 696.4 0 57 34 75 696.4 0 75 35 62 696.4 0 62 36 80 696.4 0 80 37 68 696.4 0 68 38 44 696.4 0 44 39 75 696.4 0 75 40 40 696.4 0 40 41 72 696.4 0 72 42 55 696.4 0 55 43 60 696.4 0 60 44 69 696.4 0 69 45 120 696.4 0 120 46 90 696.4 0 90 47 74 696.4 0 74 48 82 696.4 0 82 49 54 696.4 0 54 50 56 696.4 0 56 51 65 696.4 0 65 52 63 696.4 0 63 53 75 696.4 0 75 54 72 696.4 0 72 55 87 696.4 0 87 56 122 696.4 0 122 57 91 696.4 0 91 58 77 696.4 0 77 59 127 696.4 0 127 60 108 696.4 0 108 61 335 696.4 0 335 62 68 696.4 0 68 63 81 696.4 0 81 64 94 696.4 0 94 65 82 696.4 0 82 66 79 696.4 0 79 67 75 696.4 0 75 68 71 696.4 0 71 69 89 696.4 0 89 70 198 696.4 0 198 71 234 696.4 0 234 72 171 696.4 0 171 73 80 696.4 0 80 74 72 696.4 0 72 75 70 696.4 0 70 76 99 696.4 0 99 77 124 696.4 0 124 78 167 696.4 0 167 79 57 696.4 0 57 80 81 696.4 0 81 81 123 696.4 0 123 82 91 696.4 0 91 83 67 696.4 0 67 84 68 696.4 0 68 85 89 696.4 0 89 86 61 696.4 0 61 87 75 696.4 0 75 88 70 696.4 0 70 89 61 696.4 0 61 90 92 696.4 0 92 91 93 696.4 0 93 92 87 696.4 0 87 93 100 696.4 0 100 94 92 696.4 0 92 95 113 696.4 0 113 96 79 696.4 0 79 97 84 696.4 0 84 98 80 696.4 0 80 99 84 696.4 0 84 100 108 696.4 0 108 101 92 696.4 0 92 102 72 696.4 0 72 103 82 696.4 0 82 104 62 696.4 0 62 105 60 696.4 0 60 106 81 696.4 0 81 107 53 696.4 0 53 108 64 696.4 0 64 109 77 696.4 0 77 110 91 696.4 0 91 111 83 696.4 0 83 112 119 696.4 0 119 113 76 696.4 0 76 114 61 696.4 0 61 115 72 696.4 0 72 116 55 696.4 0 55 117 82 696.4 0 82 118 76 696.4 0 76 119 91 696.4 0 91 120 85 696.4 0 85 121 66 696.4 0 66 122 60 696.4 0 60 123 51 696.4 0 51 124 76 696.4 0 76 125 60 696.4 0 60 126 70 696.4 0 70 127 92 696.4 0 92 128 91 696.4 0 91 129 87 696.4 0 87 130 128 696.4 0 128 131 114 696.4 0 114 132 136 696.4 0 136 133 83 696.4 0 83 134 100 696.4 0 100 135 113 696.4 0 113 136 73 696.4 0 73 137 86 696.4 0 86 138 129 696.4 0 129 139 162 696.4 0 162 140 133 696.4 0 133 141 93 696.4 0 93 142 94 696.4 0 94 143 91 696.4 0 91 144 152 696.4 0 152 145 170 696.4 0 170 146 78 696.4 0 78 147 76 696.4 0 76 148 64 696.4 0 64 149 65 696.4 0 65 150 57 696.4 0 57 151 166 696.4 0 166 152 118 696.4 0 118 153 85 696.4 0 85 154 68 696.4 0 68 155 89 696.4 0 89 156 116 696.4 0 116 157 101 696.4 0 101 158 105 696.4 0 105 159 78 696.4 0 78 160 67 696.4 0 67 161 77 696.4 0 77 162 71 696.4 0 71 163 71 696.4 0 71 164 55 696.4 0 55 165 75 696.4 0 75 166 65 696.4 0 65 167 59 696.4 0 59 168 81 696.4 0 81 169 69 696.4 0 69 170 55 696.4 0 55 171 75 696.4 0 75 172 54 696.4 0 54 173 66 696.4 0 66 174 82 696.4 0 82 175 62 696.4 0 62 176 53 696.4 0 53 177 64 696.4 0 64 178 66 696.4 0 66 179 67 696.4 0 67 180 65 696.4 0 65 181 60 696.4 0 60 182 72 696.4 0 72 183 99 696.4 0 99 184 65 696.4 0 65 185 74 696.4 0 74 186 63 696.4 0 63 187 78 696.4 0 78 188 77 696.4 0 77 189 71 696.4 0 71 190 93 696.4 0 93 191 209 696.4 0 209 192 255 696.4 0 255 193 197 696.4 0 197 194 106 696.4 0 106 195 102 696.4 0 102 196 98 696.4 0 98 197 330 696.4 0 330 198 349 696.4 0 349 199 319 696.4 0 319 200 82 696.4 0 82 201 91 696.4 0 91 202 83 696.4 0 83 203 56 696.4 0 56 204 94 696.4 0 94 205 97 696.4 0 97 206 82 696.4 0 82 207 71 696.4 0 71 208 70 696.4 0 70 209 75 696.4 0 75 210 73 696.4 0 73 211 66 696.4 0 66 212 65 696.4 0 65 213 86 696.4 0 86 214 87 696.4 0 87 215 95 696.4 0 95 216 87 696.4 0 87 217 85 696.4 0 85 218 80 696.4 0 80 219 70 696.4 0 70 220 87 696.4 0 87 221 110 696.4 0 110 222 143 696.4 0 143 223 262 696.4 0 262 224 188 696.4 0 188 225 105 696.4 0 105 226 84 696.4 0 84 227 71 696.4 0 71 228 56 696.4 0 56 229 73 696.4 0 73 230 68 696.4 0 68 231 108 696.4 0 108 232 79 696.4 0 79 233 97 696.4 0 97 234 109 696.4 0 109 235 98 696.4 0 98 236 81 696.4 0 81 237 81 696.4 0 81 238 116 696.4 0 116 239 111 696.4 0 111 240 111 696.4 0 111 241 76 696.4 0 76 242 79 696.4 0 79 243 73 696.4 0 73 244 73 696.4 0 73 245 116 696.4 0 116 246 140 696.4 0 140 247 145 696.4 0 145 248 87 696.4 0 87 249 71 696.4 0 71 250 81 696.4 0 81 251 85 696.4 0 85 252 81 696.4 0 81 253 77 696.4 0 77 254 157 696.4 0 157 255 153 696.4 0 153 256 224 696.4 0 224 257 117 696.4 0 117 258 133 696.4 0 133 259 115 696.4 0 115 260 171 696.4 0 171 261 217 696.4 0 217 262 140 696.4 0 140 263 146 696.4 0 146 264 154 696.4 0 154 265 366 696.4 0 366 266 466 696.4 0 466 267 500 696.4 0 500 268 188 696.4 0 188 269 76 696.4 0 76 270 83 696.4 0 83 271 110 696.4 0 110 272 118 696.4 0 118 273 114 696.4 0 114 274 151 696.4 0 151 275 90 696.4 0 90 276 77 696.4 0 77 277 101 696.4 0 101 278 153 696.4 0 153 279 199 696.4 0 199 280 225 696.4 0 225 281 285 696.4 0 285 282 243 696.4 0 243 283 209 696.4 0 209 284 229 696.4 0 229 285 291 696.4 0 291 286 246 696.4 0 246 287 299 696.4 0 299 288 426 696.4 0 426 289 556 696.4 0 556 290 745 696.4 0 745 291 1434 696.4 0 1434 292 1334 696.4 0 1334 293 1135 696.4 0 1135 294 1303 696.4 0 1303 295 1208 696.4 0 1208 296 1246 696.4 0 1246 297 2007 696.4 0 2007 298 10227 696.4 0 10227 299 7358 696.4 0 7358 300 11622 696.4 0 11622 301 4483 696.4 0 4483 Finished in 4.49 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 85,483 Reads with adapters: 37,063 (43.4%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 37,041 (43.3%) Total basepairs processed: 23,202,462 bp Total written (filtered): 10,029,501 bp (43.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37063 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 21 0.0 3 0 0 21 19 469 0.0 3 0 454 13 1 1 20 35933 0.0 4 35212 691 29 1 21 110 0.0 4 19 89 2 22 14 0.0 4 3 0 9 2 23 34 0.0 4 2 0 0 29 3 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 1 26 1 0.0 4 1 32 1 0.0 4 0 0 0 1 33 1 0.0 4 0 0 0 1 35 1 0.0 4 1 39 1 0.0 4 1 114 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 439 0.0 4 0 0 0 0 439 128 1 0.0 4 0 0 0 0 1 152 7 0.0 4 0 0 0 1 6 182 1 0.0 4 0 0 0 1 183 1 0.0 4 0 0 1 184 1 0.0 4 1 185 4 0.0 4 0 2 0 1 1 186 4 0.0 4 4 187 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 1 262 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.34 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 37,041 Reads with adapters: 7,876 (21.3%) Reads written (passing filters): 37,041 (100.0%) Total basepairs processed: 10,029,501 bp Total written (filtered): 9,954,984 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7876 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 2.1% G: 95.8% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 578.8 0 16 4 8 144.7 0 8 5 3 36.2 0 3 6 2 9.0 0 2 7 3 2.3 0 3 8 6 0.6 0 6 9 5369 0.1 0 143 5226 10 2119 0.0 1 67 2052 11 334 0.0 1 1 333 12 9 0.0 1 1 8 13 2 0.0 1 0 2 157 1 0.0 1 1 209 1 0.0 1 0 1 210 1 0.0 1 1 223 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.