This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.65 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 85,316 (3.0%) Reads that were too short: 34,816 (1.2%) Reads written (passing filters): 50,500 (1.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 11,920,302 bp (1.4%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 85316 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1037 696.4 0 1037 7 12237 696.4 0 12237 8 10778 696.4 0 10778 9 215 696.4 0 215 10 169 696.4 0 169 11 57 696.4 0 57 12 51 696.4 0 51 13 57 696.4 0 57 14 65 696.4 0 65 15 53 696.4 0 53 16 59 696.4 0 59 17 67 696.4 0 67 18 55 696.4 0 55 19 58 696.4 0 58 20 66 696.4 0 66 21 47 696.4 0 47 22 52 696.4 0 52 23 62 696.4 0 62 24 64 696.4 0 64 25 47 696.4 0 47 26 61 696.4 0 61 27 57 696.4 0 57 28 100 696.4 0 100 29 93 696.4 0 93 30 66 696.4 0 66 31 50 696.4 0 50 32 58 696.4 0 58 33 67 696.4 0 67 34 55 696.4 0 55 35 64 696.4 0 64 36 82 696.4 0 82 37 71 696.4 0 71 38 69 696.4 0 69 39 86 696.4 0 86 40 87 696.4 0 87 41 331 696.4 0 331 42 104 696.4 0 104 43 96 696.4 0 96 44 103 696.4 0 103 45 64 696.4 0 64 46 72 696.4 0 72 47 70 696.4 0 70 48 51 696.4 0 51 49 67 696.4 0 67 50 64 696.4 0 64 51 77 696.4 0 77 52 75 696.4 0 75 53 140 696.4 0 140 54 92 696.4 0 92 55 94 696.4 0 94 56 82 696.4 0 82 57 64 696.4 0 64 58 76 696.4 0 76 59 79 696.4 0 79 60 61 696.4 0 61 61 62 696.4 0 62 62 117 696.4 0 117 63 121 696.4 0 121 64 1339 696.4 0 1339 65 953 696.4 0 953 66 726 696.4 0 726 67 135 696.4 0 135 68 109 696.4 0 109 69 231 696.4 0 231 70 120 696.4 0 120 71 70 696.4 0 70 72 42 696.4 0 42 73 53 696.4 0 53 74 59 696.4 0 59 75 87 696.4 0 87 76 69 696.4 0 69 77 47 696.4 0 47 78 57 696.4 0 57 79 69 696.4 0 69 80 91 696.4 0 91 81 107 696.4 0 107 82 41 696.4 0 41 83 73 696.4 0 73 84 71 696.4 0 71 85 62 696.4 0 62 86 70 696.4 0 70 87 71 696.4 0 71 88 73 696.4 0 73 89 117 696.4 0 117 90 177 696.4 0 177 91 125 696.4 0 125 92 412 696.4 0 412 93 443 696.4 0 443 94 147 696.4 0 147 95 63 696.4 0 63 96 48 696.4 0 48 97 48 696.4 0 48 98 59 696.4 0 59 99 106 696.4 0 106 100 84 696.4 0 84 101 162 696.4 0 162 102 67 696.4 0 67 103 53 696.4 0 53 104 67 696.4 0 67 105 65 696.4 0 65 106 66 696.4 0 66 107 50 696.4 0 50 108 71 696.4 0 71 109 75 696.4 0 75 110 66 696.4 0 66 111 100 696.4 0 100 112 95 696.4 0 95 113 74 696.4 0 74 114 105 696.4 0 105 115 76 696.4 0 76 116 92 696.4 0 92 117 103 696.4 0 103 118 92 696.4 0 92 119 67 696.4 0 67 120 89 696.4 0 89 121 100 696.4 0 100 122 83 696.4 0 83 123 115 696.4 0 115 124 71 696.4 0 71 125 89 696.4 0 89 126 90 696.4 0 90 127 82 696.4 0 82 128 82 696.4 0 82 129 128 696.4 0 128 130 181 696.4 0 181 131 223 696.4 0 223 132 213 696.4 0 213 133 118 696.4 0 118 134 80 696.4 0 80 135 224 696.4 0 224 136 166 696.4 0 166 137 193 696.4 0 193 138 100 696.4 0 100 139 104 696.4 0 104 140 72 696.4 0 72 141 64 696.4 0 64 142 96 696.4 0 96 143 106 696.4 0 106 144 86 696.4 0 86 145 71 696.4 0 71 146 96 696.4 0 96 147 167 696.4 0 167 148 128 696.4 0 128 149 146 696.4 0 146 150 140 696.4 0 140 151 119 696.4 0 119 152 147 696.4 0 147 153 165 696.4 0 165 154 127 696.4 0 127 155 235 696.4 0 235 156 256 696.4 0 256 157 287 696.4 0 287 158 345 696.4 0 345 159 139 696.4 0 139 160 170 696.4 0 170 161 126 696.4 0 126 162 149 696.4 0 149 163 170 696.4 0 170 164 122 696.4 0 122 165 89 696.4 0 89 166 202 696.4 0 202 167 338 696.4 0 338 168 508 696.4 0 508 169 315 696.4 0 315 170 126 696.4 0 126 171 249 696.4 0 249 172 176 696.4 0 176 173 181 696.4 0 181 174 90 696.4 0 90 175 395 696.4 0 395 176 495 696.4 0 495 177 341 696.4 0 341 178 215 696.4 0 215 179 102 696.4 0 102 180 100 696.4 0 100 181 100 696.4 0 100 182 127 696.4 0 127 183 187 696.4 0 187 184 164 696.4 0 164 185 131 696.4 0 131 186 194 696.4 0 194 187 199 696.4 0 199 188 468 696.4 0 468 189 373 696.4 0 373 190 207 696.4 0 207 191 141 696.4 0 141 192 94 696.4 0 94 193 95 696.4 0 95 194 93 696.4 0 93 195 139 696.4 0 139 196 219 696.4 0 219 197 160 696.4 0 160 198 128 696.4 0 128 199 102 696.4 0 102 200 127 696.4 0 127 201 146 696.4 0 146 202 180 696.4 0 180 203 171 696.4 0 171 204 166 696.4 0 166 205 123 696.4 0 123 206 116 696.4 0 116 207 109 696.4 0 109 208 136 696.4 0 136 209 122 696.4 0 122 210 188 696.4 0 188 211 185 696.4 0 185 212 129 696.4 0 129 213 164 696.4 0 164 214 179 696.4 0 179 215 202 696.4 0 202 216 303 696.4 0 303 217 244 696.4 0 244 218 270 696.4 0 270 219 230 696.4 0 230 220 242 696.4 0 242 221 875 696.4 0 875 222 1179 696.4 0 1179 223 1773 696.4 0 1773 224 1009 696.4 0 1009 225 270 696.4 0 270 226 372 696.4 0 372 227 431 696.4 0 431 228 271 696.4 0 271 229 184 696.4 0 184 230 108 696.4 0 108 231 100 696.4 0 100 232 134 696.4 0 134 233 127 696.4 0 127 234 128 696.4 0 128 235 146 696.4 0 146 236 158 696.4 0 158 237 271 696.4 0 271 238 254 696.4 0 254 239 208 696.4 0 208 240 234 696.4 0 234 241 821 696.4 0 821 242 614 696.4 0 614 243 1079 696.4 0 1079 244 510 696.4 0 510 245 390 696.4 0 390 246 270 696.4 0 270 247 194 696.4 0 194 248 205 696.4 0 205 249 210 696.4 0 210 250 312 696.4 0 312 251 291 696.4 0 291 252 367 696.4 0 367 253 510 696.4 0 510 254 370 696.4 0 370 255 302 696.4 0 302 256 262 696.4 0 262 257 284 696.4 0 284 258 168 696.4 0 168 259 127 696.4 0 127 260 130 696.4 0 130 261 176 696.4 0 176 262 227 696.4 0 227 263 309 696.4 0 309 264 334 696.4 0 334 265 283 696.4 0 283 266 226 696.4 0 226 267 152 696.4 0 152 268 444 696.4 0 444 269 495 696.4 0 495 270 1340 696.4 0 1340 271 748 696.4 0 748 272 930 696.4 0 930 273 642 696.4 0 642 274 309 696.4 0 309 275 295 696.4 0 295 276 161 696.4 0 161 277 205 696.4 0 205 278 200 696.4 0 200 279 177 696.4 0 177 280 166 696.4 0 166 281 170 696.4 0 170 282 337 696.4 0 337 283 418 696.4 0 418 284 435 696.4 0 435 285 323 696.4 0 323 286 247 696.4 0 247 287 268 696.4 0 268 288 155 696.4 0 155 289 259 696.4 0 259 290 453 696.4 0 453 291 644 696.4 0 644 292 729 696.4 0 729 293 521 696.4 0 521 294 204 696.4 0 204 295 195 696.4 0 195 296 201 696.4 0 201 297 274 696.4 0 274 298 386 696.4 0 386 299 631 696.4 0 631 300 574 696.4 0 574 301 466 696.4 0 466 Finished in 2.18 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 50,500 Reads with adapters: 12,737 (25.2%) Reads that were too short: 109 (0.2%) Reads written (passing filters): 12,628 (25.0%) Total basepairs processed: 11,920,302 bp Total written (filtered): 3,475,430 bp (29.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12737 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 3 5 16 96 0.0 3 0 81 10 5 17 11081 0.0 3 10463 503 90 25 18 38 0.0 3 4 31 1 2 19 1096 0.0 3 1018 55 15 8 20 183 0.0 3 132 9 1 41 21 3 0.0 3 0 0 1 2 22 1 0.0 3 0 0 0 1 24 3 0.0 3 1 0 0 2 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 11 0.0 3 0 0 1 10 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 1 41 6 0.0 3 0 0 1 5 43 1 0.0 3 0 0 0 1 44 4 0.0 3 1 0 1 2 45 6 0.0 3 1 0 0 5 46 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 56 13 0.0 3 0 0 0 13 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 60 4 0.0 3 0 0 1 3 61 2 0.0 3 0 0 0 2 62 5 0.0 3 0 0 0 5 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 71 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 1 1 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 5 0.0 3 0 0 1 4 93 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 115 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 5 0.0 3 0 0 0 5 128 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 148 3 0.0 3 0 0 0 3 150 2 0.0 3 0 0 0 2 151 8 0.0 3 0 0 1 7 152 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 8 0.0 3 0 0 0 8 161 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 1 178 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 224 2 0.0 3 0 0 0 2 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 12,628 Reads with adapters: 273 (2.2%) Reads written (passing filters): 12,628 (100.0%) Total basepairs processed: 3,475,430 bp Total written (filtered): 3,474,593 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 273 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 30.8% C: 49.5% G: 16.5% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 263 197.3 0 263 4 6 49.3 0 6 5 1 12.3 0 1 6 2 3.1 0 2 7 1 0.8 0 1