This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.48 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 404,788 (14.2%) Reads that were too short: 242,267 (8.5%) Reads written (passing filters): 162,521 (5.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 45,136,516 bp (5.3%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 404788 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1303 696.4 0 1303 7 69405 696.4 0 69405 8 2686 696.4 0 2686 9 65829 696.4 0 65829 10 137 696.4 0 137 11 68 696.4 0 68 12 60 696.4 0 60 13 88 696.4 0 88 14 88 696.4 0 88 15 73 696.4 0 73 16 66 696.4 0 66 17 79 696.4 0 79 18 98 696.4 0 98 19 76 696.4 0 76 20 71 696.4 0 71 21 69 696.4 0 69 22 52 696.4 0 52 23 75 696.4 0 75 24 63 696.4 0 63 25 72 696.4 0 72 26 63 696.4 0 63 27 82 696.4 0 82 28 99 696.4 0 99 29 81 696.4 0 81 30 101 696.4 0 101 31 80 696.4 0 80 32 84 696.4 0 84 33 69 696.4 0 69 34 59 696.4 0 59 35 100 696.4 0 100 36 76 696.4 0 76 37 192 696.4 0 192 38 145 696.4 0 145 39 59 696.4 0 59 40 56 696.4 0 56 41 69 696.4 0 69 42 48 696.4 0 48 43 60 696.4 0 60 44 81 696.4 0 81 45 86 696.4 0 86 46 59 696.4 0 59 47 60 696.4 0 60 48 62 696.4 0 62 49 63 696.4 0 63 50 56 696.4 0 56 51 43 696.4 0 43 52 55 696.4 0 55 53 66 696.4 0 66 54 48 696.4 0 48 55 49 696.4 0 49 56 449 696.4 0 449 57 76 696.4 0 76 58 64 696.4 0 64 59 65 696.4 0 65 60 70 696.4 0 70 61 72 696.4 0 72 62 47 696.4 0 47 63 41 696.4 0 41 64 55 696.4 0 55 65 51 696.4 0 51 66 75 696.4 0 75 67 53 696.4 0 53 68 68 696.4 0 68 69 84 696.4 0 84 70 103 696.4 0 103 71 85 696.4 0 85 72 90 696.4 0 90 73 130 696.4 0 130 74 86 696.4 0 86 75 178 696.4 0 178 76 127 696.4 0 127 77 237 696.4 0 237 78 172 696.4 0 172 79 143 696.4 0 143 80 122 696.4 0 122 81 164 696.4 0 164 82 136 696.4 0 136 83 109 696.4 0 109 84 106 696.4 0 106 85 117 696.4 0 117 86 100 696.4 0 100 87 423 696.4 0 423 88 152 696.4 0 152 89 91 696.4 0 91 90 59 696.4 0 59 91 59 696.4 0 59 92 70 696.4 0 70 93 76 696.4 0 76 94 96 696.4 0 96 95 73 696.4 0 73 96 558 696.4 0 558 97 160 696.4 0 160 98 64 696.4 0 64 99 148 696.4 0 148 100 119 696.4 0 119 101 98 696.4 0 98 102 99 696.4 0 99 103 133 696.4 0 133 104 104 696.4 0 104 105 106 696.4 0 106 106 142 696.4 0 142 107 150 696.4 0 150 108 171 696.4 0 171 109 96 696.4 0 96 110 160 696.4 0 160 111 180 696.4 0 180 112 184 696.4 0 184 113 123 696.4 0 123 114 139 696.4 0 139 115 117 696.4 0 117 116 88 696.4 0 88 117 87 696.4 0 87 118 128 696.4 0 128 119 157 696.4 0 157 120 128 696.4 0 128 121 124 696.4 0 124 122 115 696.4 0 115 123 177 696.4 0 177 124 194 696.4 0 194 125 183 696.4 0 183 126 133 696.4 0 133 127 106 696.4 0 106 128 96 696.4 0 96 129 108 696.4 0 108 130 118 696.4 0 118 131 110 696.4 0 110 132 119 696.4 0 119 133 110 696.4 0 110 134 155 696.4 0 155 135 112 696.4 0 112 136 106 696.4 0 106 137 110 696.4 0 110 138 195 696.4 0 195 139 250 696.4 0 250 140 352 696.4 0 352 141 171 696.4 0 171 142 76 696.4 0 76 143 79 696.4 0 79 144 69 696.4 0 69 145 92 696.4 0 92 146 113 696.4 0 113 147 133 696.4 0 133 148 129 696.4 0 129 149 83 696.4 0 83 150 71 696.4 0 71 151 43 696.4 0 43 152 72 696.4 0 72 153 101 696.4 0 101 154 110 696.4 0 110 155 148 696.4 0 148 156 131 696.4 0 131 157 122 696.4 0 122 158 145 696.4 0 145 159 986 696.4 0 986 160 1016 696.4 0 1016 161 1129 696.4 0 1129 162 79 696.4 0 79 163 82 696.4 0 82 164 101 696.4 0 101 165 71 696.4 0 71 166 92 696.4 0 92 167 101 696.4 0 101 168 84 696.4 0 84 169 81 696.4 0 81 170 81 696.4 0 81 171 57 696.4 0 57 172 115 696.4 0 115 173 105 696.4 0 105 174 123 696.4 0 123 175 112 696.4 0 112 176 127 696.4 0 127 177 65 696.4 0 65 178 53 696.4 0 53 179 78 696.4 0 78 180 99 696.4 0 99 181 91 696.4 0 91 182 93 696.4 0 93 183 89 696.4 0 89 184 81 696.4 0 81 185 118 696.4 0 118 186 106 696.4 0 106 187 100 696.4 0 100 188 77 696.4 0 77 189 72 696.4 0 72 190 51 696.4 0 51 191 65 696.4 0 65 192 81 696.4 0 81 193 103 696.4 0 103 194 110 696.4 0 110 195 107 696.4 0 107 196 73 696.4 0 73 197 96 696.4 0 96 198 92 696.4 0 92 199 121 696.4 0 121 200 92 696.4 0 92 201 83 696.4 0 83 202 110 696.4 0 110 203 107 696.4 0 107 204 82 696.4 0 82 205 140 696.4 0 140 206 216 696.4 0 216 207 385 696.4 0 385 208 236 696.4 0 236 209 253 696.4 0 253 210 197 696.4 0 197 211 153 696.4 0 153 212 421 696.4 0 421 213 300 696.4 0 300 214 302 696.4 0 302 215 99 696.4 0 99 216 95 696.4 0 95 217 129 696.4 0 129 218 414 696.4 0 414 219 184 696.4 0 184 220 135 696.4 0 135 221 178 696.4 0 178 222 146 696.4 0 146 223 149 696.4 0 149 224 133 696.4 0 133 225 185 696.4 0 185 226 105 696.4 0 105 227 207 696.4 0 207 228 443 696.4 0 443 229 181 696.4 0 181 230 146 696.4 0 146 231 192 696.4 0 192 232 913 696.4 0 913 233 602 696.4 0 602 234 903 696.4 0 903 235 176 696.4 0 176 236 146 696.4 0 146 237 241 696.4 0 241 238 463 696.4 0 463 239 186 696.4 0 186 240 358 696.4 0 358 241 232 696.4 0 232 242 270 696.4 0 270 243 309 696.4 0 309 244 194 696.4 0 194 245 145 696.4 0 145 246 126 696.4 0 126 247 204 696.4 0 204 248 333 696.4 0 333 249 436 696.4 0 436 250 850 696.4 0 850 251 1051 696.4 0 1051 252 1843 696.4 0 1843 253 3982 696.4 0 3982 254 16786 696.4 0 16786 255 86900 696.4 0 86900 256 70100 696.4 0 70100 257 42618 696.4 0 42618 258 1859 696.4 0 1859 259 147 696.4 0 147 260 153 696.4 0 153 261 155 696.4 0 155 262 99 696.4 0 99 263 105 696.4 0 105 264 110 696.4 0 110 265 86 696.4 0 86 266 188 696.4 0 188 267 156 696.4 0 156 268 206 696.4 0 206 269 58 696.4 0 58 270 50 696.4 0 50 271 63 696.4 0 63 272 73 696.4 0 73 273 83 696.4 0 83 274 66 696.4 0 66 275 67 696.4 0 67 276 65 696.4 0 65 277 58 696.4 0 58 278 83 696.4 0 83 279 75 696.4 0 75 280 78 696.4 0 78 281 65 696.4 0 65 282 81 696.4 0 81 283 91 696.4 0 91 284 98 696.4 0 98 285 50 696.4 0 50 286 56 696.4 0 56 287 64 696.4 0 64 288 52 696.4 0 52 289 62 696.4 0 62 290 46 696.4 0 46 291 72 696.4 0 72 292 60 696.4 0 60 293 58 696.4 0 58 294 87 696.4 0 87 295 110 696.4 0 110 296 78 696.4 0 78 297 70 696.4 0 70 298 81 696.4 0 81 299 103 696.4 0 103 300 159 696.4 0 159 301 251 696.4 0 251 Finished in 7.64 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 162,521 Reads with adapters: 69,055 (42.5%) Reads that were too short: 79 (0.0%) Reads written (passing filters): 68,976 (42.4%) Total basepairs processed: 45,136,516 bp Total written (filtered): 18,967,483 bp (42.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 69055 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 15 0.0 2 0 0 0 15 15 66 0.0 3 0 0 56 10 16 1074 0.0 3 0 970 75 29 17 67313 0.0 3 63154 3464 558 137 18 168 0.0 3 9 123 11 25 19 200 0.0 3 25 7 106 62 20 95 0.0 3 1 1 0 93 21 3 0.0 3 0 0 0 3 27 4 0.0 3 0 0 0 4 30 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 84 1 0.0 3 1 87 1 0.0 3 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 5 0.0 3 0 0 0 5 124 1 0.0 3 1 127 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 4 0.0 3 0 0 0 4 187 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 1 209 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 1 212 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 267 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 6 0.0 3 0 0 0 6 281 8 0.0 3 0 0 0 8 282 11 0.0 3 0 0 1 10 283 4 0.0 3 0 0 0 4 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.44 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 68,976 Reads with adapters: 2,117 (3.1%) Reads written (passing filters): 68,976 (100.0%) Total basepairs processed: 18,967,483 bp Total written (filtered): 18,960,966 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2117 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.0% C: 12.5% G: 25.0% T: 5.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1992 1077.8 0 1992 4 113 269.4 0 113 5 7 67.4 0 7 10 3 0.1 1 0 3 12 2 0.0 1 0 2