This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.92 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 589,094 (20.7%) Reads that were too short: 423,609 (14.9%) Reads written (passing filters): 165,485 (5.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 45,947,022 bp (5.4%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 589094 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1026 696.4 0 1026 7 78574 696.4 0 78574 8 1804 696.4 0 1804 9 62363 696.4 0 62363 10 115 696.4 0 115 11 42 696.4 0 42 12 34 696.4 0 34 13 35 696.4 0 35 14 48 696.4 0 48 15 43 696.4 0 43 16 57 696.4 0 57 17 32 696.4 0 32 18 43 696.4 0 43 19 47 696.4 0 47 20 42 696.4 0 42 21 33 696.4 0 33 22 38 696.4 0 38 23 28 696.4 0 28 24 35 696.4 0 35 25 56 696.4 0 56 26 40 696.4 0 40 27 42 696.4 0 42 28 52 696.4 0 52 29 46 696.4 0 46 30 43 696.4 0 43 31 59 696.4 0 59 32 37 696.4 0 37 33 36 696.4 0 36 34 25 696.4 0 25 35 36 696.4 0 36 36 31 696.4 0 31 37 101 696.4 0 101 38 92 696.4 0 92 39 38 696.4 0 38 40 35 696.4 0 35 41 31 696.4 0 31 42 31 696.4 0 31 43 39 696.4 0 39 44 56 696.4 0 56 45 48 696.4 0 48 46 26 696.4 0 26 47 25 696.4 0 25 48 32 696.4 0 32 49 30 696.4 0 30 50 39 696.4 0 39 51 34 696.4 0 34 52 34 696.4 0 34 53 33 696.4 0 33 54 28 696.4 0 28 55 31 696.4 0 31 56 36 696.4 0 36 57 24 696.4 0 24 58 28 696.4 0 28 59 30 696.4 0 30 60 51 696.4 0 51 61 36 696.4 0 36 62 23 696.4 0 23 63 25 696.4 0 25 64 29 696.4 0 29 65 40 696.4 0 40 66 54 696.4 0 54 67 51 696.4 0 51 68 58 696.4 0 58 69 45 696.4 0 45 70 86 696.4 0 86 71 69 696.4 0 69 72 53 696.4 0 53 73 89 696.4 0 89 74 62 696.4 0 62 75 54 696.4 0 54 76 79 696.4 0 79 77 172 696.4 0 172 78 264 696.4 0 264 79 115 696.4 0 115 80 145 696.4 0 145 81 141 696.4 0 141 82 142 696.4 0 142 83 108 696.4 0 108 84 129 696.4 0 129 85 138 696.4 0 138 86 191 696.4 0 191 87 481 696.4 0 481 88 212 696.4 0 212 89 99 696.4 0 99 90 82 696.4 0 82 91 85 696.4 0 85 92 101 696.4 0 101 93 105 696.4 0 105 94 141 696.4 0 141 95 117 696.4 0 117 96 153 696.4 0 153 97 152 696.4 0 152 98 148 696.4 0 148 99 125 696.4 0 125 100 111 696.4 0 111 101 94 696.4 0 94 102 93 696.4 0 93 103 103 696.4 0 103 104 117 696.4 0 117 105 127 696.4 0 127 106 128 696.4 0 128 107 129 696.4 0 129 108 126 696.4 0 126 109 100 696.4 0 100 110 127 696.4 0 127 111 149 696.4 0 149 112 150 696.4 0 150 113 113 696.4 0 113 114 120 696.4 0 120 115 110 696.4 0 110 116 75 696.4 0 75 117 90 696.4 0 90 118 107 696.4 0 107 119 177 696.4 0 177 120 108 696.4 0 108 121 160 696.4 0 160 122 111 696.4 0 111 123 169 696.4 0 169 124 155 696.4 0 155 125 145 696.4 0 145 126 114 696.4 0 114 127 91 696.4 0 91 128 122 696.4 0 122 129 104 696.4 0 104 130 106 696.4 0 106 131 116 696.4 0 116 132 108 696.4 0 108 133 150 696.4 0 150 134 137 696.4 0 137 135 119 696.4 0 119 136 100 696.4 0 100 137 109 696.4 0 109 138 145 696.4 0 145 139 296 696.4 0 296 140 384 696.4 0 384 141 240 696.4 0 240 142 106 696.4 0 106 143 88 696.4 0 88 144 82 696.4 0 82 145 133 696.4 0 133 146 110 696.4 0 110 147 145 696.4 0 145 148 132 696.4 0 132 149 89 696.4 0 89 150 91 696.4 0 91 151 45 696.4 0 45 152 63 696.4 0 63 153 97 696.4 0 97 154 119 696.4 0 119 155 143 696.4 0 143 156 130 696.4 0 130 157 131 696.4 0 131 158 154 696.4 0 154 159 1126 696.4 0 1126 160 1260 696.4 0 1260 161 1273 696.4 0 1273 162 97 696.4 0 97 163 88 696.4 0 88 164 85 696.4 0 85 165 100 696.4 0 100 166 88 696.4 0 88 167 132 696.4 0 132 168 92 696.4 0 92 169 89 696.4 0 89 170 86 696.4 0 86 171 59 696.4 0 59 172 108 696.4 0 108 173 135 696.4 0 135 174 113 696.4 0 113 175 104 696.4 0 104 176 101 696.4 0 101 177 79 696.4 0 79 178 82 696.4 0 82 179 72 696.4 0 72 180 101 696.4 0 101 181 99 696.4 0 99 182 81 696.4 0 81 183 77 696.4 0 77 184 111 696.4 0 111 185 142 696.4 0 142 186 113 696.4 0 113 187 132 696.4 0 132 188 70 696.4 0 70 189 70 696.4 0 70 190 88 696.4 0 88 191 75 696.4 0 75 192 111 696.4 0 111 193 123 696.4 0 123 194 150 696.4 0 150 195 103 696.4 0 103 196 123 696.4 0 123 197 113 696.4 0 113 198 123 696.4 0 123 199 115 696.4 0 115 200 112 696.4 0 112 201 98 696.4 0 98 202 114 696.4 0 114 203 117 696.4 0 117 204 121 696.4 0 121 205 132 696.4 0 132 206 267 696.4 0 267 207 300 696.4 0 300 208 243 696.4 0 243 209 220 696.4 0 220 210 265 696.4 0 265 211 158 696.4 0 158 212 229 696.4 0 229 213 190 696.4 0 190 214 227 696.4 0 227 215 124 696.4 0 124 216 124 696.4 0 124 217 142 696.4 0 142 218 634 696.4 0 634 219 260 696.4 0 260 220 167 696.4 0 167 221 220 696.4 0 220 222 145 696.4 0 145 223 170 696.4 0 170 224 165 696.4 0 165 225 215 696.4 0 215 226 139 696.4 0 139 227 256 696.4 0 256 228 710 696.4 0 710 229 204 696.4 0 204 230 135 696.4 0 135 231 174 696.4 0 174 232 455 696.4 0 455 233 268 696.4 0 268 234 419 696.4 0 419 235 242 696.4 0 242 236 154 696.4 0 154 237 374 696.4 0 374 238 933 696.4 0 933 239 289 696.4 0 289 240 507 696.4 0 507 241 361 696.4 0 361 242 370 696.4 0 370 243 458 696.4 0 458 244 304 696.4 0 304 245 260 696.4 0 260 246 192 696.4 0 192 247 344 696.4 0 344 248 495 696.4 0 495 249 698 696.4 0 698 250 1281 696.4 0 1281 251 1804 696.4 0 1804 252 2969 696.4 0 2969 253 5752 696.4 0 5752 254 20731 696.4 0 20731 255 154954 696.4 0 154954 256 133411 696.4 0 133411 257 81985 696.4 0 81985 258 2671 696.4 0 2671 259 153 696.4 0 153 260 138 696.4 0 138 261 157 696.4 0 157 262 105 696.4 0 105 263 117 696.4 0 117 264 100 696.4 0 100 265 93 696.4 0 93 266 217 696.4 0 217 267 190 696.4 0 190 268 197 696.4 0 197 269 71 696.4 0 71 270 63 696.4 0 63 271 82 696.4 0 82 272 81 696.4 0 81 273 91 696.4 0 91 274 89 696.4 0 89 275 65 696.4 0 65 276 62 696.4 0 62 277 54 696.4 0 54 278 79 696.4 0 79 279 102 696.4 0 102 280 79 696.4 0 79 281 70 696.4 0 70 282 104 696.4 0 104 283 94 696.4 0 94 284 110 696.4 0 110 285 56 696.4 0 56 286 43 696.4 0 43 287 64 696.4 0 64 288 77 696.4 0 77 289 52 696.4 0 52 290 63 696.4 0 63 291 66 696.4 0 66 292 70 696.4 0 70 293 61 696.4 0 61 294 100 696.4 0 100 295 132 696.4 0 132 296 67 696.4 0 67 297 76 696.4 0 76 298 85 696.4 0 85 299 94 696.4 0 94 300 198 696.4 0 198 301 294 696.4 0 294 Finished in 8.43 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 165,485 Reads with adapters: 79,662 (48.1%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 79,639 (48.1%) Total basepairs processed: 45,947,022 bp Total written (filtered): 21,737,383 bp (47.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 79662 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 11 0.0 3 0 0 10 1 19 436 0.0 3 0 424 8 3 1 20 78129 0.0 4 76675 1397 51 3 3 21 227 0.0 4 38 187 2 22 12 0.0 4 3 0 7 1 1 23 50 0.0 4 2 0 0 45 3 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 76 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 10 0.0 4 0 0 0 0 10 127 751 0.0 4 0 0 0 0 751 128 2 0.0 4 0 0 0 0 2 132 2 0.0 4 0 0 0 0 2 136 1 0.0 4 1 137 1 0.0 4 1 157 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 230 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.74 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 79,639 Reads with adapters: 2,578 (3.2%) Reads written (passing filters): 79,639 (100.0%) Total basepairs processed: 21,737,383 bp Total written (filtered): 21,705,477 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2578 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 5.9% G: 86.6% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 139 1244.4 0 139 4 8 311.1 0 8 5 2 77.8 0 2 6 1 19.4 0 1 9 7 0.3 0 1 6 10 27 0.1 1 0 27 11 2299 0.0 1 41 2258 12 68 0.0 1 2 66 13 2 0.0 1 0 2 14 1 0.0 1 0 1 15 2 0.0 1 0 2 36 1 0.0 1 1 152 5 0.0 1 1 4 210 1 0.0 1 1 225 2 0.0 1 2 267 12 0.0 1 12 273 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.