This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.99 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 139,132 (4.9%) Reads that were too short: 34,219 (1.2%) Reads written (passing filters): 104,913 (3.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 25,483,042 bp (3.0%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 139132 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3917 696.4 0 3917 7 1505 696.4 0 1505 8 35074 696.4 0 35074 9 29414 696.4 0 29414 10 61 696.4 0 61 11 33 696.4 0 33 12 19 696.4 0 19 13 69 696.4 0 69 14 31 696.4 0 31 15 19 696.4 0 19 16 38 696.4 0 38 17 24 696.4 0 24 18 35 696.4 0 35 19 30 696.4 0 30 20 30 696.4 0 30 21 202 696.4 0 202 22 47 696.4 0 47 23 45 696.4 0 45 24 40 696.4 0 40 25 19 696.4 0 19 26 122 696.4 0 122 27 112 696.4 0 112 28 25 696.4 0 25 29 58 696.4 0 58 30 24 696.4 0 24 31 47 696.4 0 47 32 28 696.4 0 28 33 35 696.4 0 35 34 39 696.4 0 39 35 39 696.4 0 39 36 26 696.4 0 26 37 40 696.4 0 40 38 44 696.4 0 44 39 56 696.4 0 56 40 30 696.4 0 30 41 38 696.4 0 38 42 35 696.4 0 35 43 19 696.4 0 19 44 41 696.4 0 41 45 29 696.4 0 29 46 22 696.4 0 22 47 39 696.4 0 39 48 46 696.4 0 46 49 23 696.4 0 23 50 32 696.4 0 32 51 41 696.4 0 41 52 45 696.4 0 45 53 32 696.4 0 32 54 40 696.4 0 40 55 30 696.4 0 30 56 32 696.4 0 32 57 15 696.4 0 15 58 24 696.4 0 24 59 34 696.4 0 34 60 27 696.4 0 27 61 31 696.4 0 31 62 21 696.4 0 21 63 36 696.4 0 36 64 49 696.4 0 49 65 96 696.4 0 96 66 63 696.4 0 63 67 42 696.4 0 42 68 44 696.4 0 44 69 92 696.4 0 92 70 38 696.4 0 38 71 41 696.4 0 41 72 28 696.4 0 28 73 37 696.4 0 37 74 60 696.4 0 60 75 56 696.4 0 56 76 57 696.4 0 57 77 60 696.4 0 60 78 66 696.4 0 66 79 419 696.4 0 419 80 274 696.4 0 274 81 110 696.4 0 110 82 60 696.4 0 60 83 53 696.4 0 53 84 102 696.4 0 102 85 53 696.4 0 53 86 54 696.4 0 54 87 66 696.4 0 66 88 37 696.4 0 37 89 61 696.4 0 61 90 55 696.4 0 55 91 36 696.4 0 36 92 32 696.4 0 32 93 91 696.4 0 91 94 51 696.4 0 51 95 57 696.4 0 57 96 45 696.4 0 45 97 35 696.4 0 35 98 38 696.4 0 38 99 78 696.4 0 78 100 70 696.4 0 70 101 67 696.4 0 67 102 40 696.4 0 40 103 25 696.4 0 25 104 27 696.4 0 27 105 29 696.4 0 29 106 27 696.4 0 27 107 54 696.4 0 54 108 33 696.4 0 33 109 45 696.4 0 45 110 40 696.4 0 40 111 47 696.4 0 47 112 148 696.4 0 148 113 96 696.4 0 96 114 105 696.4 0 105 115 55 696.4 0 55 116 60 696.4 0 60 117 367 696.4 0 367 118 178 696.4 0 178 119 212 696.4 0 212 120 57 696.4 0 57 121 68 696.4 0 68 122 40 696.4 0 40 123 58 696.4 0 58 124 45 696.4 0 45 125 64 696.4 0 64 126 73 696.4 0 73 127 83 696.4 0 83 128 116 696.4 0 116 129 85 696.4 0 85 130 134 696.4 0 134 131 665 696.4 0 665 132 325 696.4 0 325 133 572 696.4 0 572 134 101 696.4 0 101 135 67 696.4 0 67 136 110 696.4 0 110 137 86 696.4 0 86 138 241 696.4 0 241 139 88 696.4 0 88 140 95 696.4 0 95 141 35 696.4 0 35 142 52 696.4 0 52 143 52 696.4 0 52 144 148 696.4 0 148 145 163 696.4 0 163 146 149 696.4 0 149 147 207 696.4 0 207 148 72 696.4 0 72 149 58 696.4 0 58 150 52 696.4 0 52 151 39 696.4 0 39 152 42 696.4 0 42 153 59 696.4 0 59 154 72 696.4 0 72 155 60 696.4 0 60 156 83 696.4 0 83 157 116 696.4 0 116 158 89 696.4 0 89 159 73 696.4 0 73 160 63 696.4 0 63 161 81 696.4 0 81 162 62 696.4 0 62 163 50 696.4 0 50 164 70 696.4 0 70 165 88 696.4 0 88 166 66 696.4 0 66 167 103 696.4 0 103 168 166 696.4 0 166 169 104 696.4 0 104 170 95 696.4 0 95 171 96 696.4 0 96 172 109 696.4 0 109 173 190 696.4 0 190 174 97 696.4 0 97 175 144 696.4 0 144 176 545 696.4 0 545 177 487 696.4 0 487 178 2728 696.4 0 2728 179 1526 696.4 0 1526 180 2681 696.4 0 2681 181 1883 696.4 0 1883 182 1871 696.4 0 1871 183 1489 696.4 0 1489 184 708 696.4 0 708 185 162 696.4 0 162 186 94 696.4 0 94 187 64 696.4 0 64 188 86 696.4 0 86 189 143 696.4 0 143 190 85 696.4 0 85 191 119 696.4 0 119 192 99 696.4 0 99 193 97 696.4 0 97 194 222 696.4 0 222 195 424 696.4 0 424 196 1818 696.4 0 1818 197 2435 696.4 0 2435 198 1028 696.4 0 1028 199 306 696.4 0 306 200 167 696.4 0 167 201 798 696.4 0 798 202 1125 696.4 0 1125 203 990 696.4 0 990 204 555 696.4 0 555 205 45 696.4 0 45 206 50 696.4 0 50 207 44 696.4 0 44 208 35 696.4 0 35 209 64 696.4 0 64 210 53 696.4 0 53 211 53 696.4 0 53 212 54 696.4 0 54 213 67 696.4 0 67 214 50 696.4 0 50 215 219 696.4 0 219 216 160 696.4 0 160 217 210 696.4 0 210 218 88 696.4 0 88 219 60 696.4 0 60 220 77 696.4 0 77 221 172 696.4 0 172 222 693 696.4 0 693 223 1004 696.4 0 1004 224 668 696.4 0 668 225 435 696.4 0 435 226 65 696.4 0 65 227 45 696.4 0 45 228 56 696.4 0 56 229 51 696.4 0 51 230 57 696.4 0 57 231 69 696.4 0 69 232 79 696.4 0 79 233 75 696.4 0 75 234 425 696.4 0 425 235 171 696.4 0 171 236 310 696.4 0 310 237 96 696.4 0 96 238 93 696.4 0 93 239 348 696.4 0 348 240 383 696.4 0 383 241 519 696.4 0 519 242 365 696.4 0 365 243 375 696.4 0 375 244 242 696.4 0 242 245 157 696.4 0 157 246 83 696.4 0 83 247 61 696.4 0 61 248 54 696.4 0 54 249 58 696.4 0 58 250 75 696.4 0 75 251 128 696.4 0 128 252 165 696.4 0 165 253 154 696.4 0 154 254 226 696.4 0 226 255 130 696.4 0 130 256 121 696.4 0 121 257 568 696.4 0 568 258 676 696.4 0 676 259 1728 696.4 0 1728 260 1079 696.4 0 1079 261 1116 696.4 0 1116 262 309 696.4 0 309 263 314 696.4 0 314 264 1085 696.4 0 1085 265 3349 696.4 0 3349 266 1851 696.4 0 1851 267 1588 696.4 0 1588 268 46 696.4 0 46 269 62 696.4 0 62 270 69 696.4 0 69 271 71 696.4 0 71 272 56 696.4 0 56 273 160 696.4 0 160 274 209 696.4 0 209 275 204 696.4 0 204 276 260 696.4 0 260 277 318 696.4 0 318 278 289 696.4 0 289 279 267 696.4 0 267 280 68 696.4 0 68 281 70 696.4 0 70 282 53 696.4 0 53 283 85 696.4 0 85 284 83 696.4 0 83 285 99 696.4 0 99 286 103 696.4 0 103 287 100 696.4 0 100 288 89 696.4 0 89 289 96 696.4 0 96 290 173 696.4 0 173 291 1313 696.4 0 1313 292 968 696.4 0 968 293 1389 696.4 0 1389 294 400 696.4 0 400 295 135 696.4 0 135 296 114 696.4 0 114 297 193 696.4 0 193 298 161 696.4 0 161 299 219 696.4 0 219 300 228 696.4 0 228 301 199 696.4 0 199 Finished in 4.71 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 104,913 Reads with adapters: 38,255 (36.5%) Reads that were too short: 210 (0.2%) Reads written (passing filters): 38,045 (36.3%) Total basepairs processed: 25,483,042 bp Total written (filtered): 10,468,441 bp (41.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 38255 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 21 0.0 3 0 0 11 10 16 419 0.0 3 0 345 38 36 17 33707 0.0 3 31281 1989 370 67 18 105 0.0 3 10 72 12 11 19 147 0.0 3 8 25 73 41 20 3442 0.0 3 3137 208 23 74 21 10 0.0 3 1 9 22 4 0.0 3 0 0 2 2 23 10 0.0 3 1 0 0 9 24 2 0.0 3 1 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 16 0.0 3 0 0 1 15 28 3 0.0 3 0 0 0 3 30 2 0.0 3 0 0 0 2 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 14 0.0 3 0 0 0 14 38 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 41 7 0.0 3 0 0 0 7 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 5 0.0 3 0 0 0 5 47 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 23 0.0 3 0 0 0 23 51 2 0.0 3 1 0 0 1 53 3 0.0 3 0 0 0 3 54 8 0.0 3 0 0 0 8 55 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 48 0.0 3 0 0 1 47 62 6 0.0 3 0 0 0 6 63 11 0.0 3 0 0 1 10 65 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 19 0.0 3 0 0 0 19 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 6 0.0 3 0 0 0 6 82 2 0.0 3 0 0 0 2 83 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 90 7 0.0 3 0 0 0 7 91 3 0.0 3 0 0 1 2 92 13 0.0 3 0 0 0 13 93 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 1 2 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 9 0.0 3 0 0 0 9 101 1 0.0 3 0 0 0 1 102 17 0.0 3 0 0 1 16 103 3 0.0 3 0 0 0 3 104 6 0.0 3 0 0 0 6 105 15 0.0 3 0 0 2 13 110 3 0.0 3 0 0 0 3 111 4 0.0 3 0 0 0 4 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 5 0.0 3 0 0 0 5 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 175 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 1 194 5 0.0 3 0 0 1 4 205 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 260 1 0.0 3 0 1 279 5 0.0 3 0 0 0 5 280 4 0.0 3 0 0 0 4 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.33 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 38,045 Reads with adapters: 550 (1.4%) Reads written (passing filters): 38,045 (100.0%) Total basepairs processed: 10,468,441 bp Total written (filtered): 10,466,732 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 550 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.4% C: 15.1% G: 19.8% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 512 594.5 0 512 4 17 148.6 0 17 5 21 37.2 0 21