This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.02 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 114,781 (4.0%) Reads that were too short: 20,292 (0.7%) Reads written (passing filters): 94,489 (3.3%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,275,131 bp (2.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 114781 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3845 696.4 0 3845 7 1186 696.4 0 1186 8 32858 696.4 0 32858 9 34033 696.4 0 34033 10 49 696.4 0 49 11 18 696.4 0 18 12 16 696.4 0 16 13 13 696.4 0 13 14 21 696.4 0 21 15 12 696.4 0 12 16 22 696.4 0 22 17 24 696.4 0 24 18 27 696.4 0 27 19 29 696.4 0 29 20 10 696.4 0 10 21 32 696.4 0 32 22 31 696.4 0 31 23 24 696.4 0 24 24 19 696.4 0 19 25 12 696.4 0 12 26 22 696.4 0 22 27 20 696.4 0 20 28 17 696.4 0 17 29 45 696.4 0 45 30 22 696.4 0 22 31 35 696.4 0 35 32 22 696.4 0 22 33 27 696.4 0 27 34 30 696.4 0 30 35 27 696.4 0 27 36 18 696.4 0 18 37 43 696.4 0 43 38 30 696.4 0 30 39 43 696.4 0 43 40 36 696.4 0 36 41 30 696.4 0 30 42 29 696.4 0 29 43 20 696.4 0 20 44 26 696.4 0 26 45 22 696.4 0 22 46 10 696.4 0 10 47 27 696.4 0 27 48 26 696.4 0 26 49 27 696.4 0 27 50 21 696.4 0 21 51 18 696.4 0 18 52 24 696.4 0 24 53 33 696.4 0 33 54 27 696.4 0 27 55 29 696.4 0 29 56 12 696.4 0 12 57 27 696.4 0 27 58 24 696.4 0 24 59 17 696.4 0 17 60 22 696.4 0 22 61 25 696.4 0 25 62 29 696.4 0 29 63 23 696.4 0 23 64 38 696.4 0 38 65 25 696.4 0 25 66 36 696.4 0 36 67 26 696.4 0 26 68 26 696.4 0 26 69 117 696.4 0 117 70 30 696.4 0 30 71 35 696.4 0 35 72 24 696.4 0 24 73 16 696.4 0 16 74 23 696.4 0 23 75 30 696.4 0 30 76 26 696.4 0 26 77 35 696.4 0 35 78 38 696.4 0 38 79 386 696.4 0 386 80 244 696.4 0 244 81 70 696.4 0 70 82 21 696.4 0 21 83 20 696.4 0 20 84 28 696.4 0 28 85 24 696.4 0 24 86 25 696.4 0 25 87 36 696.4 0 36 88 26 696.4 0 26 89 29 696.4 0 29 90 30 696.4 0 30 91 16 696.4 0 16 92 19 696.4 0 19 93 25 696.4 0 25 94 20 696.4 0 20 95 24 696.4 0 24 96 28 696.4 0 28 97 24 696.4 0 24 98 35 696.4 0 35 99 42 696.4 0 42 100 43 696.4 0 43 101 37 696.4 0 37 102 26 696.4 0 26 103 20 696.4 0 20 104 21 696.4 0 21 105 21 696.4 0 21 106 24 696.4 0 24 107 32 696.4 0 32 108 16 696.4 0 16 109 27 696.4 0 27 110 29 696.4 0 29 111 26 696.4 0 26 112 60 696.4 0 60 113 77 696.4 0 77 114 77 696.4 0 77 115 25 696.4 0 25 116 36 696.4 0 36 117 188 696.4 0 188 118 85 696.4 0 85 119 95 696.4 0 95 120 31 696.4 0 31 121 32 696.4 0 32 122 34 696.4 0 34 123 29 696.4 0 29 124 33 696.4 0 33 125 32 696.4 0 32 126 63 696.4 0 63 127 31 696.4 0 31 128 75 696.4 0 75 129 46 696.4 0 46 130 61 696.4 0 61 131 357 696.4 0 357 132 149 696.4 0 149 133 227 696.4 0 227 134 76 696.4 0 76 135 39 696.4 0 39 136 75 696.4 0 75 137 49 696.4 0 49 138 164 696.4 0 164 139 68 696.4 0 68 140 67 696.4 0 67 141 23 696.4 0 23 142 29 696.4 0 29 143 37 696.4 0 37 144 71 696.4 0 71 145 95 696.4 0 95 146 107 696.4 0 107 147 147 696.4 0 147 148 39 696.4 0 39 149 34 696.4 0 34 150 26 696.4 0 26 151 23 696.4 0 23 152 20 696.4 0 20 153 27 696.4 0 27 154 42 696.4 0 42 155 37 696.4 0 37 156 42 696.4 0 42 157 71 696.4 0 71 158 55 696.4 0 55 159 44 696.4 0 44 160 36 696.4 0 36 161 54 696.4 0 54 162 46 696.4 0 46 163 47 696.4 0 47 164 53 696.4 0 53 165 78 696.4 0 78 166 65 696.4 0 65 167 78 696.4 0 78 168 93 696.4 0 93 169 63 696.4 0 63 170 50 696.4 0 50 171 70 696.4 0 70 172 61 696.4 0 61 173 183 696.4 0 183 174 73 696.4 0 73 175 92 696.4 0 92 176 392 696.4 0 392 177 414 696.4 0 414 178 2478 696.4 0 2478 179 1399 696.4 0 1399 180 2611 696.4 0 2611 181 1407 696.4 0 1407 182 1441 696.4 0 1441 183 669 696.4 0 669 184 222 696.4 0 222 185 63 696.4 0 63 186 34 696.4 0 34 187 53 696.4 0 53 188 44 696.4 0 44 189 72 696.4 0 72 190 47 696.4 0 47 191 36 696.4 0 36 192 56 696.4 0 56 193 60 696.4 0 60 194 121 696.4 0 121 195 225 696.4 0 225 196 788 696.4 0 788 197 1123 696.4 0 1123 198 489 696.4 0 489 199 163 696.4 0 163 200 69 696.4 0 69 201 236 696.4 0 236 202 492 696.4 0 492 203 370 696.4 0 370 204 222 696.4 0 222 205 23 696.4 0 23 206 38 696.4 0 38 207 33 696.4 0 33 208 34 696.4 0 34 209 41 696.4 0 41 210 35 696.4 0 35 211 21 696.4 0 21 212 31 696.4 0 31 213 32 696.4 0 32 214 44 696.4 0 44 215 133 696.4 0 133 216 149 696.4 0 149 217 125 696.4 0 125 218 37 696.4 0 37 219 34 696.4 0 34 220 35 696.4 0 35 221 86 696.4 0 86 222 249 696.4 0 249 223 508 696.4 0 508 224 328 696.4 0 328 225 310 696.4 0 310 226 32 696.4 0 32 227 27 696.4 0 27 228 46 696.4 0 46 229 32 696.4 0 32 230 37 696.4 0 37 231 44 696.4 0 44 232 42 696.4 0 42 233 59 696.4 0 59 234 402 696.4 0 402 235 173 696.4 0 173 236 298 696.4 0 298 237 38 696.4 0 38 238 52 696.4 0 52 239 269 696.4 0 269 240 256 696.4 0 256 241 298 696.4 0 298 242 181 696.4 0 181 243 166 696.4 0 166 244 96 696.4 0 96 245 66 696.4 0 66 246 53 696.4 0 53 247 46 696.4 0 46 248 36 696.4 0 36 249 52 696.4 0 52 250 38 696.4 0 38 251 68 696.4 0 68 252 80 696.4 0 80 253 75 696.4 0 75 254 151 696.4 0 151 255 76 696.4 0 76 256 78 696.4 0 78 257 300 696.4 0 300 258 296 696.4 0 296 259 1165 696.4 0 1165 260 797 696.4 0 797 261 1025 696.4 0 1025 262 166 696.4 0 166 263 181 696.4 0 181 264 836 696.4 0 836 265 1525 696.4 0 1525 266 1156 696.4 0 1156 267 668 696.4 0 668 268 24 696.4 0 24 269 23 696.4 0 23 270 29 696.4 0 29 271 45 696.4 0 45 272 32 696.4 0 32 273 61 696.4 0 61 274 69 696.4 0 69 275 59 696.4 0 59 276 86 696.4 0 86 277 102 696.4 0 102 278 90 696.4 0 90 279 93 696.4 0 93 280 33 696.4 0 33 281 47 696.4 0 47 282 41 696.4 0 41 283 35 696.4 0 35 284 58 696.4 0 58 285 48 696.4 0 48 286 53 696.4 0 53 287 89 696.4 0 89 288 59 696.4 0 59 289 60 696.4 0 60 290 119 696.4 0 119 291 958 696.4 0 958 292 729 696.4 0 729 293 1112 696.4 0 1112 294 339 696.4 0 339 295 100 696.4 0 100 296 81 696.4 0 81 297 199 696.4 0 199 298 96 696.4 0 96 299 144 696.4 0 144 300 169 696.4 0 169 301 118 696.4 0 118 Finished in 4.69 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 94,489 Reads with adapters: 37,503 (39.7%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 37,482 (39.7%) Total basepairs processed: 24,275,131 bp Total written (filtered): 10,038,537 bp (41.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37503 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 9 0.0 3 0 0 9 19 282 0.0 3 0 277 4 0 1 20 35573 0.0 4 34843 693 35 2 21 76 0.0 4 15 59 1 0 1 22 7 0.0 4 0 0 6 1 23 32 0.0 4 4 2 0 22 4 24 2 0.0 4 1 0 0 1 27 1 0.0 4 0 0 1 31 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 23 0.0 4 0 0 0 0 23 127 1422 0.0 4 0 0 0 0 1422 128 6 0.0 4 0 0 0 0 6 130 47 0.0 4 0 0 0 0 47 137 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.35 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 37,482 Reads with adapters: 6,010 (16.0%) Reads written (passing filters): 37,482 (100.0%) Total basepairs processed: 10,038,537 bp Total written (filtered): 9,980,154 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6010 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 3.0% G: 94.9% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 585.7 0 8 4 4 146.4 0 4 5 1 36.6 0 1 6 3 9.2 0 3 7 1 2.3 0 1 9 3228 0.1 0 96 3132 10 2434 0.0 1 62 2372 11 316 0.0 1 3 313 12 5 0.0 1 0 5 13 2 0.0 1 0 2 138 1 0.0 1 1 150 2 0.0 1 0 2 152 1 0.0 1 0 1 169 2 0.0 1 2 208 1 0.0 1 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.