This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.90 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 175,693 (6.2%) Reads that were too short: 84,882 (3.0%) Reads written (passing filters): 90,811 (3.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,645,673 bp (2.8%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 175693 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4268 696.4 0 4268 7 30783 696.4 0 30783 8 24844 696.4 0 24844 9 71 696.4 0 71 10 96 696.4 0 96 11 15 696.4 0 15 12 19 696.4 0 19 13 27 696.4 0 27 14 18 696.4 0 18 15 28 696.4 0 28 16 17 696.4 0 17 17 26 696.4 0 26 18 20 696.4 0 20 19 25 696.4 0 25 20 64 696.4 0 64 21 38 696.4 0 38 22 14 696.4 0 14 23 18 696.4 0 18 24 15 696.4 0 15 25 19 696.4 0 19 26 10 696.4 0 10 27 14 696.4 0 14 28 12 696.4 0 12 29 31 696.4 0 31 30 70 696.4 0 70 31 13 696.4 0 13 32 14 696.4 0 14 33 15 696.4 0 15 34 15 696.4 0 15 35 21 696.4 0 21 36 16 696.4 0 16 37 10 696.4 0 10 38 13 696.4 0 13 39 18 696.4 0 18 40 31 696.4 0 31 41 15 696.4 0 15 42 16 696.4 0 16 43 26 696.4 0 26 44 14 696.4 0 14 45 22 696.4 0 22 46 30 696.4 0 30 47 76 696.4 0 76 48 33 696.4 0 33 49 30 696.4 0 30 50 80 696.4 0 80 51 66 696.4 0 66 52 35 696.4 0 35 53 13 696.4 0 13 54 15 696.4 0 15 55 29 696.4 0 29 56 18 696.4 0 18 57 22 696.4 0 22 58 28 696.4 0 28 59 39 696.4 0 39 60 56 696.4 0 56 61 76 696.4 0 76 62 152 696.4 0 152 63 52 696.4 0 52 64 81 696.4 0 81 65 144 696.4 0 144 66 39 696.4 0 39 67 44 696.4 0 44 68 131 696.4 0 131 69 112 696.4 0 112 70 160 696.4 0 160 71 153 696.4 0 153 72 191 696.4 0 191 73 391 696.4 0 391 74 700 696.4 0 700 75 7636 696.4 0 7636 76 4220 696.4 0 4220 77 4898 696.4 0 4898 78 38 696.4 0 38 79 17 696.4 0 17 80 19 696.4 0 19 81 22 696.4 0 22 82 22 696.4 0 22 83 20 696.4 0 20 84 38 696.4 0 38 85 24 696.4 0 24 86 28 696.4 0 28 87 23 696.4 0 23 88 52 696.4 0 52 89 28 696.4 0 28 90 42 696.4 0 42 91 37 696.4 0 37 92 32 696.4 0 32 93 13 696.4 0 13 94 13 696.4 0 13 95 32 696.4 0 32 96 20 696.4 0 20 97 15 696.4 0 15 98 32 696.4 0 32 99 17 696.4 0 17 100 12 696.4 0 12 101 19 696.4 0 19 102 14 696.4 0 14 103 16 696.4 0 16 104 17 696.4 0 17 105 14 696.4 0 14 106 18 696.4 0 18 107 17 696.4 0 17 108 13 696.4 0 13 109 20 696.4 0 20 110 22 696.4 0 22 111 10 696.4 0 10 112 13 696.4 0 13 113 13 696.4 0 13 114 24 696.4 0 24 115 18 696.4 0 18 116 16 696.4 0 16 117 28 696.4 0 28 118 30 696.4 0 30 119 27 696.4 0 27 120 27 696.4 0 27 121 28 696.4 0 28 122 21 696.4 0 21 123 21 696.4 0 21 124 23 696.4 0 23 125 183 696.4 0 183 126 133 696.4 0 133 127 127 696.4 0 127 128 56 696.4 0 56 129 88 696.4 0 88 130 53 696.4 0 53 131 51 696.4 0 51 132 57 696.4 0 57 133 37 696.4 0 37 134 37 696.4 0 37 135 46 696.4 0 46 136 53 696.4 0 53 137 83 696.4 0 83 138 50 696.4 0 50 139 34 696.4 0 34 140 17 696.4 0 17 141 28 696.4 0 28 142 39 696.4 0 39 143 33 696.4 0 33 144 17 696.4 0 17 145 8 696.4 0 8 146 27 696.4 0 27 147 19 696.4 0 19 148 20 696.4 0 20 149 36 696.4 0 36 150 48 696.4 0 48 151 18 696.4 0 18 152 28 696.4 0 28 153 33 696.4 0 33 154 28 696.4 0 28 155 26 696.4 0 26 156 27 696.4 0 27 157 27 696.4 0 27 158 21 696.4 0 21 159 14 696.4 0 14 160 24 696.4 0 24 161 65 696.4 0 65 162 40 696.4 0 40 163 83 696.4 0 83 164 38 696.4 0 38 165 57 696.4 0 57 166 159 696.4 0 159 167 153 696.4 0 153 168 352 696.4 0 352 169 177 696.4 0 177 170 43 696.4 0 43 171 50 696.4 0 50 172 25 696.4 0 25 173 32 696.4 0 32 174 27 696.4 0 27 175 25 696.4 0 25 176 38 696.4 0 38 177 28 696.4 0 28 178 38 696.4 0 38 179 33 696.4 0 33 180 60 696.4 0 60 181 52 696.4 0 52 182 51 696.4 0 51 183 87 696.4 0 87 184 77 696.4 0 77 185 66 696.4 0 66 186 228 696.4 0 228 187 180 696.4 0 180 188 289 696.4 0 289 189 159 696.4 0 159 190 67 696.4 0 67 191 92 696.4 0 92 192 111 696.4 0 111 193 600 696.4 0 600 194 534 696.4 0 534 195 596 696.4 0 596 196 371 696.4 0 371 197 804 696.4 0 804 198 586 696.4 0 586 199 670 696.4 0 670 200 261 696.4 0 261 201 145 696.4 0 145 202 102 696.4 0 102 203 294 696.4 0 294 204 294 696.4 0 294 205 322 696.4 0 322 206 85 696.4 0 85 207 39 696.4 0 39 208 38 696.4 0 38 209 49 696.4 0 49 210 58 696.4 0 58 211 101 696.4 0 101 212 85 696.4 0 85 213 137 696.4 0 137 214 145 696.4 0 145 215 174 696.4 0 174 216 102 696.4 0 102 217 311 696.4 0 311 218 283 696.4 0 283 219 263 696.4 0 263 220 188 696.4 0 188 221 89 696.4 0 89 222 71 696.4 0 71 223 153 696.4 0 153 224 195 696.4 0 195 225 76 696.4 0 76 226 68 696.4 0 68 227 63 696.4 0 63 228 70 696.4 0 70 229 54 696.4 0 54 230 66 696.4 0 66 231 97 696.4 0 97 232 132 696.4 0 132 233 222 696.4 0 222 234 313 696.4 0 313 235 430 696.4 0 430 236 569 696.4 0 569 237 727 696.4 0 727 238 7531 696.4 0 7531 239 4559 696.4 0 4559 240 4213 696.4 0 4213 241 189 696.4 0 189 242 247 696.4 0 247 243 253 696.4 0 253 244 241 696.4 0 241 245 285 696.4 0 285 246 506 696.4 0 506 247 3382 696.4 0 3382 248 3505 696.4 0 3505 249 9747 696.4 0 9747 250 11736 696.4 0 11736 251 6176 696.4 0 6176 252 264 696.4 0 264 253 242 696.4 0 242 254 214 696.4 0 214 255 182 696.4 0 182 256 108 696.4 0 108 257 109 696.4 0 109 258 63 696.4 0 63 259 111 696.4 0 111 260 51 696.4 0 51 261 75 696.4 0 75 262 76 696.4 0 76 263 129 696.4 0 129 264 87 696.4 0 87 265 133 696.4 0 133 266 76 696.4 0 76 267 86 696.4 0 86 268 183 696.4 0 183 269 222 696.4 0 222 270 158 696.4 0 158 271 171 696.4 0 171 272 158 696.4 0 158 273 145 696.4 0 145 274 120 696.4 0 120 275 129 696.4 0 129 276 145 696.4 0 145 277 251 696.4 0 251 278 338 696.4 0 338 279 675 696.4 0 675 280 736 696.4 0 736 281 823 696.4 0 823 282 2432 696.4 0 2432 283 2782 696.4 0 2782 284 5058 696.4 0 5058 285 2750 696.4 0 2750 286 598 696.4 0 598 287 497 696.4 0 497 288 313 696.4 0 313 289 211 696.4 0 211 290 146 696.4 0 146 291 238 696.4 0 238 292 257 696.4 0 257 293 187 696.4 0 187 294 400 696.4 0 400 295 637 696.4 0 637 296 707 696.4 0 707 297 877 696.4 0 877 298 501 696.4 0 501 299 478 696.4 0 478 300 300 696.4 0 300 301 218 696.4 0 218 Finished in 4.21 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 90,811 Reads with adapters: 30,308 (33.4%) Reads that were too short: 218 (0.2%) Reads written (passing filters): 30,090 (33.1%) Total basepairs processed: 23,645,673 bp Total written (filtered): 8,217,920 bp (34.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30308 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 14 0.0 3 0 0 11 3 16 239 0.0 3 0 210 17 12 17 28852 0.0 3 27044 1504 252 52 18 90 0.0 3 8 74 5 3 19 79 0.0 3 2 0 40 37 20 68 0.0 3 0 0 0 68 24 1 0.0 3 0 0 0 1 25 5 0.0 3 0 0 0 5 27 1 0.0 3 0 0 0 1 29 3 0.0 3 1 0 0 2 30 3 0.0 3 0 0 0 3 31 4 0.0 3 0 0 1 3 38 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 45 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 47 12 0.0 3 0 0 0 12 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 13 0.0 3 0 0 0 13 67 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 77 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 5 0.0 3 0 0 0 5 90 13 0.0 3 0 0 0 13 91 39 0.0 3 0 0 0 39 92 8 0.0 3 0 0 0 8 93 5 0.0 3 0 0 0 5 94 11 0.0 3 0 0 1 10 95 3 0.0 3 0 0 0 3 96 158 0.0 3 0 0 1 157 97 8 0.0 3 0 0 0 8 98 26 0.0 3 0 0 1 25 99 27 0.0 3 0 0 1 26 100 6 0.0 3 0 0 0 6 101 2 0.0 3 0 0 0 2 102 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 6 0.0 3 0 0 2 4 111 3 0.0 3 0 0 0 3 112 2 0.0 3 0 0 0 2 113 4 0.0 3 0 0 0 4 114 3 0.0 3 0 0 0 3 115 14 0.0 3 0 0 1 13 116 253 0.0 3 0 0 1 252 117 72 0.0 3 0 0 1 71 118 2 0.0 3 0 0 0 2 119 28 0.0 3 0 0 1 27 120 10 0.0 3 0 1 1 8 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 5 0.0 3 0 0 0 5 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 167 8 0.0 3 0 0 0 8 168 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 194 6 0.0 3 0 0 0 6 195 1 0.0 3 0 0 0 1 196 4 0.0 3 0 0 1 3 199 4 0.0 3 0 0 0 4 203 4 0.0 3 0 0 0 4 205 1 0.0 3 0 0 0 1 206 5 0.0 3 0 0 0 5 207 1 0.0 3 0 0 0 1 208 9 0.0 3 0 0 0 9 209 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 11 0.0 3 0 0 0 11 218 5 0.0 3 0 0 0 5 219 3 0.0 3 0 0 0 3 221 2 0.0 3 0 0 0 2 222 2 0.0 3 0 0 1 1 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 5 0.0 3 0 0 0 5 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 4 0.0 3 0 0 0 4 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 3 0.0 3 0 0 0 3 287 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.06 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 30,090 Reads with adapters: 269 (0.9%) Reads written (passing filters): 30,090 (100.0%) Total basepairs processed: 8,217,920 bp Total written (filtered): 8,217,086 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 269 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.0% C: 60.6% G: 19.0% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 255 470.2 0 255 4 5 117.5 0 5 5 5 29.4 0 5 6 4 7.3 0 4