This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.30 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 163,015 (5.7%) Reads that were too short: 83,847 (2.9%) Reads written (passing filters): 79,168 (2.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 21,691,145 bp (2.5%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 163015 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4336 696.4 0 4336 7 30263 696.4 0 30263 8 27837 696.4 0 27837 9 37 696.4 0 37 10 31 696.4 0 31 11 7 696.4 0 7 12 17 696.4 0 17 13 6 696.4 0 6 14 14 696.4 0 14 15 16 696.4 0 16 16 17 696.4 0 17 17 16 696.4 0 16 18 13 696.4 0 13 19 20 696.4 0 20 20 11 696.4 0 11 21 17 696.4 0 17 22 8 696.4 0 8 23 21 696.4 0 21 24 20 696.4 0 20 25 17 696.4 0 17 26 9 696.4 0 9 27 5 696.4 0 5 28 13 696.4 0 13 29 21 696.4 0 21 30 12 696.4 0 12 31 11 696.4 0 11 32 8 696.4 0 8 33 16 696.4 0 16 34 10 696.4 0 10 35 21 696.4 0 21 36 11 696.4 0 11 37 8 696.4 0 8 38 12 696.4 0 12 39 17 696.4 0 17 40 11 696.4 0 11 41 15 696.4 0 15 42 16 696.4 0 16 43 19 696.4 0 19 44 17 696.4 0 17 45 20 696.4 0 20 46 21 696.4 0 21 47 11 696.4 0 11 48 35 696.4 0 35 49 18 696.4 0 18 50 27 696.4 0 27 51 21 696.4 0 21 52 27 696.4 0 27 53 9 696.4 0 9 54 31 696.4 0 31 55 26 696.4 0 26 56 17 696.4 0 17 57 19 696.4 0 19 58 25 696.4 0 25 59 25 696.4 0 25 60 32 696.4 0 32 61 48 696.4 0 48 62 103 696.4 0 103 63 43 696.4 0 43 64 61 696.4 0 61 65 73 696.4 0 73 66 21 696.4 0 21 67 30 696.4 0 30 68 57 696.4 0 57 69 74 696.4 0 74 70 54 696.4 0 54 71 44 696.4 0 44 72 73 696.4 0 73 73 136 696.4 0 136 74 237 696.4 0 237 75 3965 696.4 0 3965 76 2384 696.4 0 2384 77 3198 696.4 0 3198 78 24 696.4 0 24 79 13 696.4 0 13 80 9 696.4 0 9 81 18 696.4 0 18 82 16 696.4 0 16 83 17 696.4 0 17 84 14 696.4 0 14 85 12 696.4 0 12 86 9 696.4 0 9 87 19 696.4 0 19 88 21 696.4 0 21 89 16 696.4 0 16 90 14 696.4 0 14 91 32 696.4 0 32 92 16 696.4 0 16 93 22 696.4 0 22 94 21 696.4 0 21 95 45 696.4 0 45 96 16 696.4 0 16 97 17 696.4 0 17 98 32 696.4 0 32 99 9 696.4 0 9 100 10 696.4 0 10 101 22 696.4 0 22 102 23 696.4 0 23 103 22 696.4 0 22 104 19 696.4 0 19 105 25 696.4 0 25 106 19 696.4 0 19 107 7 696.4 0 7 108 24 696.4 0 24 109 22 696.4 0 22 110 22 696.4 0 22 111 17 696.4 0 17 112 25 696.4 0 25 113 16 696.4 0 16 114 24 696.4 0 24 115 23 696.4 0 23 116 18 696.4 0 18 117 24 696.4 0 24 118 22 696.4 0 22 119 19 696.4 0 19 120 16 696.4 0 16 121 23 696.4 0 23 122 18 696.4 0 18 123 24 696.4 0 24 124 23 696.4 0 23 125 150 696.4 0 150 126 126 696.4 0 126 127 89 696.4 0 89 128 21 696.4 0 21 129 35 696.4 0 35 130 22 696.4 0 22 131 13 696.4 0 13 132 42 696.4 0 42 133 27 696.4 0 27 134 30 696.4 0 30 135 34 696.4 0 34 136 35 696.4 0 35 137 34 696.4 0 34 138 55 696.4 0 55 139 37 696.4 0 37 140 17 696.4 0 17 141 20 696.4 0 20 142 25 696.4 0 25 143 31 696.4 0 31 144 11 696.4 0 11 145 19 696.4 0 19 146 38 696.4 0 38 147 13 696.4 0 13 148 13 696.4 0 13 149 16 696.4 0 16 150 37 696.4 0 37 151 19 696.4 0 19 152 13 696.4 0 13 153 16 696.4 0 16 154 26 696.4 0 26 155 18 696.4 0 18 156 24 696.4 0 24 157 14 696.4 0 14 158 28 696.4 0 28 159 16 696.4 0 16 160 21 696.4 0 21 161 29 696.4 0 29 162 17 696.4 0 17 163 39 696.4 0 39 164 29 696.4 0 29 165 23 696.4 0 23 166 35 696.4 0 35 167 68 696.4 0 68 168 94 696.4 0 94 169 58 696.4 0 58 170 45 696.4 0 45 171 30 696.4 0 30 172 27 696.4 0 27 173 26 696.4 0 26 174 20 696.4 0 20 175 16 696.4 0 16 176 27 696.4 0 27 177 20 696.4 0 20 178 23 696.4 0 23 179 26 696.4 0 26 180 26 696.4 0 26 181 25 696.4 0 25 182 32 696.4 0 32 183 35 696.4 0 35 184 42 696.4 0 42 185 29 696.4 0 29 186 83 696.4 0 83 187 69 696.4 0 69 188 94 696.4 0 94 189 69 696.4 0 69 190 30 696.4 0 30 191 39 696.4 0 39 192 56 696.4 0 56 193 178 696.4 0 178 194 128 696.4 0 128 195 117 696.4 0 117 196 109 696.4 0 109 197 481 696.4 0 481 198 326 696.4 0 326 199 315 696.4 0 315 200 115 696.4 0 115 201 73 696.4 0 73 202 46 696.4 0 46 203 106 696.4 0 106 204 99 696.4 0 99 205 77 696.4 0 77 206 35 696.4 0 35 207 27 696.4 0 27 208 27 696.4 0 27 209 19 696.4 0 19 210 39 696.4 0 39 211 69 696.4 0 69 212 36 696.4 0 36 213 41 696.4 0 41 214 64 696.4 0 64 215 45 696.4 0 45 216 47 696.4 0 47 217 122 696.4 0 122 218 223 696.4 0 223 219 135 696.4 0 135 220 130 696.4 0 130 221 72 696.4 0 72 222 40 696.4 0 40 223 86 696.4 0 86 224 179 696.4 0 179 225 79 696.4 0 79 226 63 696.4 0 63 227 40 696.4 0 40 228 56 696.4 0 56 229 38 696.4 0 38 230 52 696.4 0 52 231 87 696.4 0 87 232 97 696.4 0 97 233 155 696.4 0 155 234 259 696.4 0 259 235 439 696.4 0 439 236 600 696.4 0 600 237 663 696.4 0 663 238 9409 696.4 0 9409 239 6264 696.4 0 6264 240 5804 696.4 0 5804 241 176 696.4 0 176 242 223 696.4 0 223 243 271 696.4 0 271 244 222 696.4 0 222 245 249 696.4 0 249 246 384 696.4 0 384 247 3714 696.4 0 3714 248 3639 696.4 0 3639 249 11464 696.4 0 11464 250 13960 696.4 0 13960 251 7714 696.4 0 7714 252 71 696.4 0 71 253 52 696.4 0 52 254 45 696.4 0 45 255 26 696.4 0 26 256 19 696.4 0 19 257 28 696.4 0 28 258 46 696.4 0 46 259 86 696.4 0 86 260 21 696.4 0 21 261 23 696.4 0 23 262 34 696.4 0 34 263 65 696.4 0 65 264 40 696.4 0 40 265 54 696.4 0 54 266 33 696.4 0 33 267 49 696.4 0 49 268 99 696.4 0 99 269 124 696.4 0 124 270 68 696.4 0 68 271 120 696.4 0 120 272 123 696.4 0 123 273 74 696.4 0 74 274 40 696.4 0 40 275 91 696.4 0 91 276 76 696.4 0 76 277 265 696.4 0 265 278 152 696.4 0 152 279 410 696.4 0 410 280 420 696.4 0 420 281 469 696.4 0 469 282 1825 696.4 0 1825 283 1583 696.4 0 1583 284 4142 696.4 0 4142 285 1069 696.4 0 1069 286 238 696.4 0 238 287 201 696.4 0 201 288 104 696.4 0 104 289 56 696.4 0 56 290 59 696.4 0 59 291 76 696.4 0 76 292 70 696.4 0 70 293 68 696.4 0 68 294 424 696.4 0 424 295 655 696.4 0 655 296 537 696.4 0 537 297 660 696.4 0 660 298 189 696.4 0 189 299 304 696.4 0 304 300 327 696.4 0 327 301 152 696.4 0 152 Finished in 4.17 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 79,168 Reads with adapters: 35,116 (44.4%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 35,095 (44.3%) Total basepairs processed: 21,691,145 bp Total written (filtered): 9,477,181 bp (43.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35116 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 9 0.0 3 0 0 8 1 19 407 0.0 3 0 393 13 1 20 30028 0.0 4 29472 540 13 2 1 21 114 0.0 4 20 90 4 22 3490 0.0 4 3439 45 5 0 1 23 31 0.0 4 0 6 1 22 2 25 5 0.0 4 0 0 0 4 1 26 1 0.0 4 0 0 0 0 1 59 27 0.0 4 0 0 0 0 27 72 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 961 0.0 4 0 0 0 0 961 128 5 0.0 4 0 0 0 0 5 135 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 1 170 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 1 269 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.23 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 35,095 Reads with adapters: 2,676 (7.6%) Reads written (passing filters): 35,095 (100.0%) Total basepairs processed: 9,477,181 bp Total written (filtered): 9,448,699 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2676 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 3.4% G: 93.4% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 25 548.4 0 25 4 9 137.1 0 9 5 1 34.3 0 1 7 1 2.1 0 1 9 60 0.1 0 0 60 10 712 0.0 1 24 688 11 1857 0.0 1 12 1845 12 9 0.0 1 0 9 13 1 0.0 1 0 1 151 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.