This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.49 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 102,642 (3.6%) Reads that were too short: 20,134 (0.7%) Reads written (passing filters): 82,508 (2.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 19,555,331 bp (2.3%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 102642 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 694 696.4 0 694 7 2795 696.4 0 2795 8 23902 696.4 0 23902 9 21356 696.4 0 21356 10 116 696.4 0 116 11 30 696.4 0 30 12 36 696.4 0 36 13 24 696.4 0 24 14 48 696.4 0 48 15 19 696.4 0 19 16 26 696.4 0 26 17 31 696.4 0 31 18 30 696.4 0 30 19 28 696.4 0 28 20 37 696.4 0 37 21 36 696.4 0 36 22 19 696.4 0 19 23 37 696.4 0 37 24 24 696.4 0 24 25 36 696.4 0 36 26 27 696.4 0 27 27 30 696.4 0 30 28 34 696.4 0 34 29 70 696.4 0 70 30 33 696.4 0 33 31 30 696.4 0 30 32 26 696.4 0 26 33 33 696.4 0 33 34 28 696.4 0 28 35 60 696.4 0 60 36 59 696.4 0 59 37 44 696.4 0 44 38 37 696.4 0 37 39 54 696.4 0 54 40 54 696.4 0 54 41 38 696.4 0 38 42 38 696.4 0 38 43 41 696.4 0 41 44 30 696.4 0 30 45 30 696.4 0 30 46 41 696.4 0 41 47 26 696.4 0 26 48 34 696.4 0 34 49 44 696.4 0 44 50 36 696.4 0 36 51 35 696.4 0 35 52 49 696.4 0 49 53 63 696.4 0 63 54 73 696.4 0 73 55 40 696.4 0 40 56 40 696.4 0 40 57 37 696.4 0 37 58 67 696.4 0 67 59 67 696.4 0 67 60 38 696.4 0 38 61 39 696.4 0 39 62 34 696.4 0 34 63 92 696.4 0 92 64 115 696.4 0 115 65 64 696.4 0 64 66 38 696.4 0 38 67 65 696.4 0 65 68 92 696.4 0 92 69 78 696.4 0 78 70 54 696.4 0 54 71 84 696.4 0 84 72 458 696.4 0 458 73 1836 696.4 0 1836 74 1351 696.4 0 1351 75 658 696.4 0 658 76 42 696.4 0 42 77 29 696.4 0 29 78 32 696.4 0 32 79 61 696.4 0 61 80 33 696.4 0 33 81 31 696.4 0 31 82 32 696.4 0 32 83 45 696.4 0 45 84 64 696.4 0 64 85 64 696.4 0 64 86 93 696.4 0 93 87 92 696.4 0 92 88 270 696.4 0 270 89 88 696.4 0 88 90 63 696.4 0 63 91 52 696.4 0 52 92 98 696.4 0 98 93 46 696.4 0 46 94 43 696.4 0 43 95 60 696.4 0 60 96 40 696.4 0 40 97 28 696.4 0 28 98 38 696.4 0 38 99 55 696.4 0 55 100 75 696.4 0 75 101 50 696.4 0 50 102 40 696.4 0 40 103 29 696.4 0 29 104 40 696.4 0 40 105 67 696.4 0 67 106 42 696.4 0 42 107 41 696.4 0 41 108 81 696.4 0 81 109 69 696.4 0 69 110 73 696.4 0 73 111 47 696.4 0 47 112 31 696.4 0 31 113 41 696.4 0 41 114 34 696.4 0 34 115 55 696.4 0 55 116 33 696.4 0 33 117 36 696.4 0 36 118 41 696.4 0 41 119 32 696.4 0 32 120 35 696.4 0 35 121 35 696.4 0 35 122 43 696.4 0 43 123 32 696.4 0 32 124 46 696.4 0 46 125 52 696.4 0 52 126 57 696.4 0 57 127 59 696.4 0 59 128 95 696.4 0 95 129 90 696.4 0 90 130 107 696.4 0 107 131 53 696.4 0 53 132 49 696.4 0 49 133 60 696.4 0 60 134 60 696.4 0 60 135 99 696.4 0 99 136 108 696.4 0 108 137 110 696.4 0 110 138 60 696.4 0 60 139 40 696.4 0 40 140 43 696.4 0 43 141 75 696.4 0 75 142 72 696.4 0 72 143 53 696.4 0 53 144 126 696.4 0 126 145 225 696.4 0 225 146 334 696.4 0 334 147 557 696.4 0 557 148 293 696.4 0 293 149 151 696.4 0 151 150 129 696.4 0 129 151 52 696.4 0 52 152 51 696.4 0 51 153 98 696.4 0 98 154 106 696.4 0 106 155 271 696.4 0 271 156 212 696.4 0 212 157 361 696.4 0 361 158 653 696.4 0 653 159 442 696.4 0 442 160 465 696.4 0 465 161 99 696.4 0 99 162 194 696.4 0 194 163 92 696.4 0 92 164 63 696.4 0 63 165 144 696.4 0 144 166 207 696.4 0 207 167 376 696.4 0 376 168 161 696.4 0 161 169 128 696.4 0 128 170 171 696.4 0 171 171 156 696.4 0 156 172 140 696.4 0 140 173 201 696.4 0 201 174 2023 696.4 0 2023 175 1409 696.4 0 1409 176 917 696.4 0 917 177 146 696.4 0 146 178 85 696.4 0 85 179 70 696.4 0 70 180 148 696.4 0 148 181 159 696.4 0 159 182 356 696.4 0 356 183 146 696.4 0 146 184 161 696.4 0 161 185 389 696.4 0 389 186 494 696.4 0 494 187 3629 696.4 0 3629 188 3604 696.4 0 3604 189 1437 696.4 0 1437 190 68 696.4 0 68 191 59 696.4 0 59 192 74 696.4 0 74 193 56 696.4 0 56 194 64 696.4 0 64 195 69 696.4 0 69 196 50 696.4 0 50 197 64 696.4 0 64 198 60 696.4 0 60 199 65 696.4 0 65 200 93 696.4 0 93 201 98 696.4 0 98 202 108 696.4 0 108 203 170 696.4 0 170 204 177 696.4 0 177 205 192 696.4 0 192 206 123 696.4 0 123 207 80 696.4 0 80 208 56 696.4 0 56 209 116 696.4 0 116 210 65 696.4 0 65 211 44 696.4 0 44 212 50 696.4 0 50 213 56 696.4 0 56 214 105 696.4 0 105 215 94 696.4 0 94 216 77 696.4 0 77 217 57 696.4 0 57 218 75 696.4 0 75 219 82 696.4 0 82 220 112 696.4 0 112 221 94 696.4 0 94 222 72 696.4 0 72 223 107 696.4 0 107 224 80 696.4 0 80 225 70 696.4 0 70 226 63 696.4 0 63 227 145 696.4 0 145 228 139 696.4 0 139 229 93 696.4 0 93 230 93 696.4 0 93 231 121 696.4 0 121 232 145 696.4 0 145 233 99 696.4 0 99 234 92 696.4 0 92 235 122 696.4 0 122 236 158 696.4 0 158 237 152 696.4 0 152 238 152 696.4 0 152 239 114 696.4 0 114 240 116 696.4 0 116 241 66 696.4 0 66 242 76 696.4 0 76 243 71 696.4 0 71 244 98 696.4 0 98 245 105 696.4 0 105 246 116 696.4 0 116 247 251 696.4 0 251 248 331 696.4 0 331 249 451 696.4 0 451 250 475 696.4 0 475 251 776 696.4 0 776 252 799 696.4 0 799 253 474 696.4 0 474 254 199 696.4 0 199 255 133 696.4 0 133 256 103 696.4 0 103 257 100 696.4 0 100 258 129 696.4 0 129 259 165 696.4 0 165 260 398 696.4 0 398 261 919 696.4 0 919 262 1083 696.4 0 1083 263 645 696.4 0 645 264 245 696.4 0 245 265 90 696.4 0 90 266 67 696.4 0 67 267 88 696.4 0 88 268 162 696.4 0 162 269 228 696.4 0 228 270 172 696.4 0 172 271 159 696.4 0 159 272 172 696.4 0 172 273 167 696.4 0 167 274 159 696.4 0 159 275 122 696.4 0 122 276 104 696.4 0 104 277 150 696.4 0 150 278 209 696.4 0 209 279 253 696.4 0 253 280 165 696.4 0 165 281 383 696.4 0 383 282 378 696.4 0 378 283 251 696.4 0 251 284 145 696.4 0 145 285 251 696.4 0 251 286 212 696.4 0 212 287 109 696.4 0 109 288 145 696.4 0 145 289 134 696.4 0 134 290 276 696.4 0 276 291 387 696.4 0 387 292 377 696.4 0 377 293 237 696.4 0 237 294 175 696.4 0 175 295 260 696.4 0 260 296 193 696.4 0 193 297 269 696.4 0 269 298 334 696.4 0 334 299 345 696.4 0 345 300 258 696.4 0 258 301 274 696.4 0 274 Finished in 3.71 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 82,508 Reads with adapters: 25,184 (30.5%) Reads that were too short: 164 (0.2%) Reads written (passing filters): 25,020 (30.3%) Total basepairs processed: 19,555,331 bp Total written (filtered): 6,894,737 bp (35.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25184 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 13 0.0 3 0 0 8 5 16 257 0.0 3 0 205 20 32 17 24454 0.0 3 23024 1204 179 47 18 65 0.0 3 11 45 6 3 19 56 0.0 3 2 2 30 22 20 105 0.0 3 0 0 1 104 25 9 0.0 3 0 0 0 9 26 2 0.0 3 0 0 2 28 1 0.0 3 0 0 0 1 30 4 0.0 3 0 0 1 3 31 2 0.0 3 0 0 0 2 32 4 0.0 3 0 0 0 4 35 1 0.0 3 0 0 0 1 36 6 0.0 3 0 0 0 6 39 1 0.0 3 1 41 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 1 44 2 0.0 3 0 1 1 45 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 1 2 52 2 0.0 3 0 0 0 2 53 5 0.0 3 0 0 0 5 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 1 57 4 0.0 3 0 0 0 4 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 5 0.0 3 0 0 0 5 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 1 91 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 97 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 6 0.0 3 0 0 0 6 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 9 0.0 3 0 0 1 8 107 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 3 0.0 3 0 0 0 3 131 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 1 194 4 0.0 3 0 0 1 3 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 1 205 2 0.0 3 0 0 0 2 206 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 217 25 0.0 3 0 0 2 23 218 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 6 0.0 3 0 0 0 6 281 7 0.0 3 0 0 0 7 282 3 0.0 3 0 0 0 3 283 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 289 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.89 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 25,020 Reads with adapters: 735 (2.9%) Reads written (passing filters): 25,020 (100.0%) Total basepairs processed: 6,894,737 bp Total written (filtered): 6,892,512 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 735 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.7% C: 18.9% G: 9.5% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 717 390.9 0 717 4 16 97.7 0 16 5 2 24.4 0 2