This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.26 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 80,064 (2.8%) Reads that were too short: 7,628 (0.3%) Reads written (passing filters): 72,436 (2.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 18,861,506 bp (2.2%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 80064 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 705 696.4 0 705 7 2233 696.4 0 2233 8 22600 696.4 0 22600 9 25083 696.4 0 25083 10 37 696.4 0 37 11 19 696.4 0 19 12 28 696.4 0 28 13 19 696.4 0 19 14 32 696.4 0 32 15 25 696.4 0 25 16 20 696.4 0 20 17 11 696.4 0 11 18 27 696.4 0 27 19 28 696.4 0 28 20 19 696.4 0 19 21 26 696.4 0 26 22 32 696.4 0 32 23 29 696.4 0 29 24 34 696.4 0 34 25 28 696.4 0 28 26 19 696.4 0 19 27 26 696.4 0 26 28 33 696.4 0 33 29 28 696.4 0 28 30 34 696.4 0 34 31 38 696.4 0 38 32 23 696.4 0 23 33 15 696.4 0 15 34 21 696.4 0 21 35 24 696.4 0 24 36 35 696.4 0 35 37 23 696.4 0 23 38 25 696.4 0 25 39 30 696.4 0 30 40 29 696.4 0 29 41 27 696.4 0 27 42 28 696.4 0 28 43 20 696.4 0 20 44 18 696.4 0 18 45 22 696.4 0 22 46 40 696.4 0 40 47 30 696.4 0 30 48 15 696.4 0 15 49 34 696.4 0 34 50 32 696.4 0 32 51 23 696.4 0 23 52 28 696.4 0 28 53 52 696.4 0 52 54 3181 696.4 0 3181 55 228 696.4 0 228 56 27 696.4 0 27 57 28 696.4 0 28 58 42 696.4 0 42 59 33 696.4 0 33 60 23 696.4 0 23 61 31 696.4 0 31 62 25 696.4 0 25 63 32 696.4 0 32 64 42 696.4 0 42 65 24 696.4 0 24 66 30 696.4 0 30 67 50 696.4 0 50 68 54 696.4 0 54 69 68 696.4 0 68 70 61 696.4 0 61 71 98 696.4 0 98 72 571 696.4 0 571 73 2217 696.4 0 2217 74 1490 696.4 0 1490 75 609 696.4 0 609 76 25 696.4 0 25 77 23 696.4 0 23 78 21 696.4 0 21 79 41 696.4 0 41 80 18 696.4 0 18 81 18 696.4 0 18 82 26 696.4 0 26 83 21 696.4 0 21 84 22 696.4 0 22 85 44 696.4 0 44 86 49 696.4 0 49 87 50 696.4 0 50 88 82 696.4 0 82 89 50 696.4 0 50 90 36 696.4 0 36 91 28 696.4 0 28 92 31 696.4 0 31 93 33 696.4 0 33 94 35 696.4 0 35 95 37 696.4 0 37 96 32 696.4 0 32 97 17 696.4 0 17 98 23 696.4 0 23 99 28 696.4 0 28 100 34 696.4 0 34 101 37 696.4 0 37 102 25 696.4 0 25 103 24 696.4 0 24 104 28 696.4 0 28 105 42 696.4 0 42 106 29 696.4 0 29 107 32 696.4 0 32 108 53 696.4 0 53 109 36 696.4 0 36 110 45 696.4 0 45 111 30 696.4 0 30 112 16 696.4 0 16 113 27 696.4 0 27 114 29 696.4 0 29 115 38 696.4 0 38 116 38 696.4 0 38 117 16 696.4 0 16 118 34 696.4 0 34 119 38 696.4 0 38 120 23 696.4 0 23 121 33 696.4 0 33 122 58 696.4 0 58 123 35 696.4 0 35 124 23 696.4 0 23 125 30 696.4 0 30 126 26 696.4 0 26 127 31 696.4 0 31 128 51 696.4 0 51 129 70 696.4 0 70 130 39 696.4 0 39 131 37 696.4 0 37 132 27 696.4 0 27 133 45 696.4 0 45 134 27 696.4 0 27 135 44 696.4 0 44 136 26 696.4 0 26 137 34 696.4 0 34 138 27 696.4 0 27 139 30 696.4 0 30 140 37 696.4 0 37 141 35 696.4 0 35 142 38 696.4 0 38 143 38 696.4 0 38 144 86 696.4 0 86 145 259 696.4 0 259 146 189 696.4 0 189 147 430 696.4 0 430 148 104 696.4 0 104 149 125 696.4 0 125 150 90 696.4 0 90 151 17 696.4 0 17 152 31 696.4 0 31 153 38 696.4 0 38 154 39 696.4 0 39 155 75 696.4 0 75 156 77 696.4 0 77 157 96 696.4 0 96 158 242 696.4 0 242 159 165 696.4 0 165 160 179 696.4 0 179 161 39 696.4 0 39 162 94 696.4 0 94 163 33 696.4 0 33 164 36 696.4 0 36 165 27 696.4 0 27 166 40 696.4 0 40 167 60 696.4 0 60 168 50 696.4 0 50 169 70 696.4 0 70 170 68 696.4 0 68 171 70 696.4 0 70 172 71 696.4 0 71 173 67 696.4 0 67 174 901 696.4 0 901 175 511 696.4 0 511 176 391 696.4 0 391 177 54 696.4 0 54 178 37 696.4 0 37 179 27 696.4 0 27 180 56 696.4 0 56 181 80 696.4 0 80 182 226 696.4 0 226 183 61 696.4 0 61 184 67 696.4 0 67 185 92 696.4 0 92 186 151 696.4 0 151 187 1338 696.4 0 1338 188 1416 696.4 0 1416 189 545 696.4 0 545 190 40 696.4 0 40 191 42 696.4 0 42 192 44 696.4 0 44 193 29 696.4 0 29 194 51 696.4 0 51 195 31 696.4 0 31 196 36 696.4 0 36 197 38 696.4 0 38 198 33 696.4 0 33 199 26 696.4 0 26 200 32 696.4 0 32 201 38 696.4 0 38 202 44 696.4 0 44 203 67 696.4 0 67 204 61 696.4 0 61 205 77 696.4 0 77 206 47 696.4 0 47 207 32 696.4 0 32 208 34 696.4 0 34 209 26 696.4 0 26 210 23 696.4 0 23 211 18 696.4 0 18 212 36 696.4 0 36 213 36 696.4 0 36 214 31 696.4 0 31 215 38 696.4 0 38 216 30 696.4 0 30 217 41 696.4 0 41 218 33 696.4 0 33 219 45 696.4 0 45 220 40 696.4 0 40 221 30 696.4 0 30 222 30 696.4 0 30 223 45 696.4 0 45 224 37 696.4 0 37 225 35 696.4 0 35 226 21 696.4 0 21 227 62 696.4 0 62 228 68 696.4 0 68 229 46 696.4 0 46 230 52 696.4 0 52 231 59 696.4 0 59 232 65 696.4 0 65 233 46 696.4 0 46 234 39 696.4 0 39 235 43 696.4 0 43 236 56 696.4 0 56 237 67 696.4 0 67 238 60 696.4 0 60 239 57 696.4 0 57 240 41 696.4 0 41 241 42 696.4 0 42 242 32 696.4 0 32 243 27 696.4 0 27 244 30 696.4 0 30 245 35 696.4 0 35 246 48 696.4 0 48 247 47 696.4 0 47 248 103 696.4 0 103 249 100 696.4 0 100 250 188 696.4 0 188 251 471 696.4 0 471 252 358 696.4 0 358 253 152 696.4 0 152 254 63 696.4 0 63 255 37 696.4 0 37 256 31 696.4 0 31 257 34 696.4 0 34 258 45 696.4 0 45 259 57 696.4 0 57 260 150 696.4 0 150 261 235 696.4 0 235 262 278 696.4 0 278 263 191 696.4 0 191 264 105 696.4 0 105 265 37 696.4 0 37 266 30 696.4 0 30 267 37 696.4 0 37 268 75 696.4 0 75 269 87 696.4 0 87 270 62 696.4 0 62 271 53 696.4 0 53 272 67 696.4 0 67 273 63 696.4 0 63 274 66 696.4 0 66 275 91 696.4 0 91 276 57 696.4 0 57 277 69 696.4 0 69 278 86 696.4 0 86 279 70 696.4 0 70 280 85 696.4 0 85 281 74 696.4 0 74 282 105 696.4 0 105 283 74 696.4 0 74 284 45 696.4 0 45 285 161 696.4 0 161 286 65 696.4 0 65 287 49 696.4 0 49 288 68 696.4 0 68 289 72 696.4 0 72 290 67 696.4 0 67 291 103 696.4 0 103 292 92 696.4 0 92 293 74 696.4 0 74 294 93 696.4 0 93 295 162 696.4 0 162 296 74 696.4 0 74 297 135 696.4 0 135 298 100 696.4 0 100 299 96 696.4 0 96 300 135 696.4 0 135 301 172 696.4 0 172 Finished in 3.74 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 72,436 Reads with adapters: 27,184 (37.5%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 27,169 (37.5%) Total basepairs processed: 18,861,506 bp Total written (filtered): 7,384,947 bp (39.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27184 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 13 0.0 3 0 0 13 19 308 0.0 3 0 304 3 0 1 20 26493 0.0 4 25948 519 24 2 21 73 0.0 4 11 59 2 0 1 22 4 0.0 4 0 1 3 23 200 0.0 4 182 4 0 12 2 24 2 0.0 4 0 1 0 0 1 55 2 0.0 4 0 0 0 0 2 72 1 0.0 4 1 85 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 1 111 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 67 0.0 4 0 0 0 0 67 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 3 0.0 4 0 0 0 0 3 188 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 243 1 0.0 4 0 0 0 0 1 288 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.98 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 27,169 Reads with adapters: 5,774 (21.3%) Reads written (passing filters): 27,169 (100.0%) Total basepairs processed: 7,384,947 bp Total written (filtered): 7,328,271 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5774 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 2.1% G: 95.1% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 424.5 0 5 4 2 106.1 0 2 6 1 6.6 0 1 9 4050 0.1 0 131 3919 10 1288 0.0 1 48 1240 11 353 0.0 1 3 350 12 8 0.0 1 0 8 17 1 0.0 1 1 18 25 0.0 1 20 5 19 8 0.0 1 8 20 2 0.0 1 1 1 21 6 0.0 1 3 3 22 6 0.0 1 3 3 99 13 0.0 1 12 1 150 2 0.0 1 0 2 184 1 0.0 1 1 186 1 0.0 1 0 1 208 1 0.0 1 0 1 256 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.