This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.67 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 80,599 (2.8%) Reads that were too short: 20,684 (0.7%) Reads written (passing filters): 59,915 (2.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 13,837,540 bp (1.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 80599 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 763 696.4 0 763 7 16259 696.4 0 16259 8 14705 696.4 0 14705 9 39 696.4 0 39 10 66 696.4 0 66 11 238 696.4 0 238 12 220 696.4 0 220 13 440 696.4 0 440 14 491 696.4 0 491 15 391 696.4 0 391 16 25 696.4 0 25 17 16 696.4 0 16 18 8 696.4 0 8 19 12 696.4 0 12 20 8 696.4 0 8 21 15 696.4 0 15 22 11 696.4 0 11 23 27 696.4 0 27 24 40 696.4 0 40 25 147 696.4 0 147 26 178 696.4 0 178 27 65 696.4 0 65 28 12 696.4 0 12 29 14 696.4 0 14 30 18 696.4 0 18 31 22 696.4 0 22 32 16 696.4 0 16 33 17 696.4 0 17 34 18 696.4 0 18 35 18 696.4 0 18 36 20 696.4 0 20 37 13 696.4 0 13 38 24 696.4 0 24 39 16 696.4 0 16 40 14 696.4 0 14 41 14 696.4 0 14 42 17 696.4 0 17 43 15 696.4 0 15 44 22 696.4 0 22 45 18 696.4 0 18 46 13 696.4 0 13 47 23 696.4 0 23 48 24 696.4 0 24 49 14 696.4 0 14 50 19 696.4 0 19 51 16 696.4 0 16 52 21 696.4 0 21 53 30 696.4 0 30 54 18 696.4 0 18 55 29 696.4 0 29 56 19 696.4 0 19 57 37 696.4 0 37 58 23 696.4 0 23 59 21 696.4 0 21 60 38 696.4 0 38 61 70 696.4 0 70 62 38 696.4 0 38 63 21 696.4 0 21 64 13 696.4 0 13 65 14 696.4 0 14 66 28 696.4 0 28 67 24 696.4 0 24 68 13 696.4 0 13 69 18 696.4 0 18 70 25 696.4 0 25 71 30 696.4 0 30 72 29 696.4 0 29 73 27 696.4 0 27 74 40 696.4 0 40 75 35 696.4 0 35 76 47 696.4 0 47 77 37 696.4 0 37 78 306 696.4 0 306 79 60 696.4 0 60 80 77 696.4 0 77 81 84 696.4 0 84 82 505 696.4 0 505 83 470 696.4 0 470 84 298 696.4 0 298 85 27 696.4 0 27 86 34 696.4 0 34 87 50 696.4 0 50 88 23 696.4 0 23 89 42 696.4 0 42 90 34 696.4 0 34 91 62 696.4 0 62 92 24 696.4 0 24 93 35 696.4 0 35 94 59 696.4 0 59 95 63 696.4 0 63 96 51 696.4 0 51 97 78 696.4 0 78 98 41 696.4 0 41 99 22 696.4 0 22 100 38 696.4 0 38 101 44 696.4 0 44 102 70 696.4 0 70 103 67 696.4 0 67 104 71 696.4 0 71 105 67 696.4 0 67 106 59 696.4 0 59 107 59 696.4 0 59 108 36 696.4 0 36 109 32 696.4 0 32 110 98 696.4 0 98 111 200 696.4 0 200 112 75 696.4 0 75 113 81 696.4 0 81 114 56 696.4 0 56 115 55 696.4 0 55 116 49 696.4 0 49 117 30 696.4 0 30 118 68 696.4 0 68 119 88 696.4 0 88 120 126 696.4 0 126 121 73 696.4 0 73 122 272 696.4 0 272 123 191 696.4 0 191 124 138 696.4 0 138 125 41 696.4 0 41 126 49 696.4 0 49 127 74 696.4 0 74 128 68 696.4 0 68 129 57 696.4 0 57 130 53 696.4 0 53 131 59 696.4 0 59 132 59 696.4 0 59 133 85 696.4 0 85 134 83 696.4 0 83 135 84 696.4 0 84 136 141 696.4 0 141 137 330 696.4 0 330 138 240 696.4 0 240 139 448 696.4 0 448 140 198 696.4 0 198 141 145 696.4 0 145 142 119 696.4 0 119 143 42 696.4 0 42 144 72 696.4 0 72 145 87 696.4 0 87 146 96 696.4 0 96 147 108 696.4 0 108 148 115 696.4 0 115 149 75 696.4 0 75 150 92 696.4 0 92 151 78 696.4 0 78 152 82 696.4 0 82 153 101 696.4 0 101 154 89 696.4 0 89 155 160 696.4 0 160 156 96 696.4 0 96 157 192 696.4 0 192 158 229 696.4 0 229 159 256 696.4 0 256 160 1448 696.4 0 1448 161 1211 696.4 0 1211 162 1538 696.4 0 1538 163 510 696.4 0 510 164 148 696.4 0 148 165 93 696.4 0 93 166 81 696.4 0 81 167 65 696.4 0 65 168 130 696.4 0 130 169 96 696.4 0 96 170 100 696.4 0 100 171 60 696.4 0 60 172 64 696.4 0 64 173 105 696.4 0 105 174 100 696.4 0 100 175 130 696.4 0 130 176 144 696.4 0 144 177 117 696.4 0 117 178 94 696.4 0 94 179 129 696.4 0 129 180 513 696.4 0 513 181 774 696.4 0 774 182 924 696.4 0 924 183 567 696.4 0 567 184 228 696.4 0 228 185 189 696.4 0 189 186 183 696.4 0 183 187 1415 696.4 0 1415 188 2349 696.4 0 2349 189 1110 696.4 0 1110 190 119 696.4 0 119 191 66 696.4 0 66 192 75 696.4 0 75 193 72 696.4 0 72 194 78 696.4 0 78 195 65 696.4 0 65 196 92 696.4 0 92 197 91 696.4 0 91 198 101 696.4 0 101 199 106 696.4 0 106 200 120 696.4 0 120 201 98 696.4 0 98 202 190 696.4 0 190 203 168 696.4 0 168 204 225 696.4 0 225 205 200 696.4 0 200 206 212 696.4 0 212 207 208 696.4 0 208 208 127 696.4 0 127 209 91 696.4 0 91 210 671 696.4 0 671 211 660 696.4 0 660 212 1334 696.4 0 1334 213 450 696.4 0 450 214 168 696.4 0 168 215 109 696.4 0 109 216 86 696.4 0 86 217 101 696.4 0 101 218 108 696.4 0 108 219 192 696.4 0 192 220 303 696.4 0 303 221 155 696.4 0 155 222 89 696.4 0 89 223 83 696.4 0 83 224 92 696.4 0 92 225 126 696.4 0 126 226 128 696.4 0 128 227 174 696.4 0 174 228 135 696.4 0 135 229 221 696.4 0 221 230 1273 696.4 0 1273 231 796 696.4 0 796 232 1169 696.4 0 1169 233 126 696.4 0 126 234 58 696.4 0 58 235 58 696.4 0 58 236 65 696.4 0 65 237 50 696.4 0 50 238 75 696.4 0 75 239 92 696.4 0 92 240 126 696.4 0 126 241 94 696.4 0 94 242 76 696.4 0 76 243 64 696.4 0 64 244 74 696.4 0 74 245 88 696.4 0 88 246 60 696.4 0 60 247 55 696.4 0 55 248 60 696.4 0 60 249 44 696.4 0 44 250 47 696.4 0 47 251 54 696.4 0 54 252 85 696.4 0 85 253 105 696.4 0 105 254 96 696.4 0 96 255 90 696.4 0 90 256 103 696.4 0 103 257 116 696.4 0 116 258 164 696.4 0 164 259 136 696.4 0 136 260 213 696.4 0 213 261 140 696.4 0 140 262 127 696.4 0 127 263 97 696.4 0 97 264 95 696.4 0 95 265 60 696.4 0 60 266 91 696.4 0 91 267 95 696.4 0 95 268 123 696.4 0 123 269 135 696.4 0 135 270 79 696.4 0 79 271 88 696.4 0 88 272 129 696.4 0 129 273 200 696.4 0 200 274 208 696.4 0 208 275 523 696.4 0 523 276 583 696.4 0 583 277 345 696.4 0 345 278 209 696.4 0 209 279 192 696.4 0 192 280 333 696.4 0 333 281 177 696.4 0 177 282 114 696.4 0 114 283 237 696.4 0 237 284 335 696.4 0 335 285 216 696.4 0 216 286 90 696.4 0 90 287 125 696.4 0 125 288 158 696.4 0 158 289 349 696.4 0 349 290 230 696.4 0 230 291 144 696.4 0 144 292 81 696.4 0 81 293 101 696.4 0 101 294 134 696.4 0 134 295 105 696.4 0 105 296 91 696.4 0 91 297 148 696.4 0 148 298 143 696.4 0 143 299 140 696.4 0 140 300 398 696.4 0 398 301 798 696.4 0 798 Finished in 2.64 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 59,915 Reads with adapters: 16,538 (27.6%) Reads that were too short: 289 (0.5%) Reads written (passing filters): 16,249 (27.1%) Total basepairs processed: 13,837,540 bp Total written (filtered): 4,475,749 bp (32.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16538 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 19 0.0 3 0 0 6 13 16 201 0.0 3 0 170 24 7 17 15713 0.0 3 14813 742 128 30 18 62 0.0 3 3 44 5 10 19 32 0.0 3 6 1 14 11 20 66 0.0 3 2 0 2 62 22 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 4 0.0 3 0 0 0 4 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 33 9 0.0 3 0 0 0 9 34 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 36 5 0.0 3 0 0 2 3 37 15 0.0 3 0 0 0 15 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 3 0.0 3 0 0 0 3 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 54 20 0.0 3 0 0 0 20 55 1 0.0 3 0 0 0 1 57 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 62 6 0.0 3 0 0 0 6 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 8 0.0 3 0 0 3 5 70 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 1 78 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 3 0.0 3 0 0 1 2 110 3 0.0 3 0 0 0 3 111 2 0.0 3 0 0 0 2 112 4 0.0 3 0 0 0 4 113 8 0.0 3 0 0 1 7 114 9 0.0 3 0 0 0 9 115 2 0.0 3 0 0 0 2 120 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 123 20 0.0 3 0 0 0 20 124 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 126 12 0.0 3 0 0 2 10 127 1 0.0 3 0 0 0 1 131 4 0.0 3 0 0 0 4 132 4 0.0 3 0 0 0 4 133 4 0.0 3 0 0 0 4 135 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 3 0.0 3 0 0 0 3 141 45 0.0 3 0 0 0 45 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 160 5 0.0 3 0 0 1 4 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 9 0.0 3 0 0 0 9 181 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 205 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 101 0.0 3 0 0 5 96 218 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 250 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 276 2 0.0 3 0 0 0 2 277 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.57 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 16,249 Reads with adapters: 299 (1.8%) Reads written (passing filters): 16,249 (100.0%) Total basepairs processed: 4,475,749 bp Total written (filtered): 4,474,842 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 299 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.2% C: 28.4% G: 12.7% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 291 253.9 0 291 4 6 63.5 0 6 5 2 15.9 0 2