This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.03 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 65,088 (2.3%) Reads that were too short: 12,028 (0.4%) Reads written (passing filters): 53,060 (1.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 12,954,878 bp (1.5%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 65088 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 542 696.4 0 542 7 15449 696.4 0 15449 8 16623 696.4 0 16623 9 24 696.4 0 24 10 23 696.4 0 23 11 60 696.4 0 60 12 61 696.4 0 61 13 107 696.4 0 107 14 117 696.4 0 117 15 118 696.4 0 118 16 15 696.4 0 15 17 11 696.4 0 11 18 8 696.4 0 8 19 8 696.4 0 8 20 9 696.4 0 9 21 10 696.4 0 10 22 5 696.4 0 5 23 8 696.4 0 8 24 11 696.4 0 11 25 1391 696.4 0 1391 26 86 696.4 0 86 27 16 696.4 0 16 28 10 696.4 0 10 29 10 696.4 0 10 30 9 696.4 0 9 31 8 696.4 0 8 32 3 696.4 0 3 33 6 696.4 0 6 34 12 696.4 0 12 35 9 696.4 0 9 36 4 696.4 0 4 37 7 696.4 0 7 38 10 696.4 0 10 39 6 696.4 0 6 40 14 696.4 0 14 41 9 696.4 0 9 42 13 696.4 0 13 43 12 696.4 0 12 44 12 696.4 0 12 45 12 696.4 0 12 46 11 696.4 0 11 47 20 696.4 0 20 48 12 696.4 0 12 49 15 696.4 0 15 50 21 696.4 0 21 51 11 696.4 0 11 52 4 696.4 0 4 53 14 696.4 0 14 54 19 696.4 0 19 55 25 696.4 0 25 56 17 696.4 0 17 57 21 696.4 0 21 58 19 696.4 0 19 59 16 696.4 0 16 60 16 696.4 0 16 61 55 696.4 0 55 62 14 696.4 0 14 63 12 696.4 0 12 64 10 696.4 0 10 65 14 696.4 0 14 66 12 696.4 0 12 67 13 696.4 0 13 68 12 696.4 0 12 69 18 696.4 0 18 70 14 696.4 0 14 71 13 696.4 0 13 72 25 696.4 0 25 73 16 696.4 0 16 74 17 696.4 0 17 75 12 696.4 0 12 76 18 696.4 0 18 77 18 696.4 0 18 78 30 696.4 0 30 79 63 696.4 0 63 80 68 696.4 0 68 81 125 696.4 0 125 82 772 696.4 0 772 83 761 696.4 0 761 84 496 696.4 0 496 85 46 696.4 0 46 86 51 696.4 0 51 87 51 696.4 0 51 88 55 696.4 0 55 89 62 696.4 0 62 90 56 696.4 0 56 91 49 696.4 0 49 92 32 696.4 0 32 93 53 696.4 0 53 94 45 696.4 0 45 95 68 696.4 0 68 96 39 696.4 0 39 97 51 696.4 0 51 98 46 696.4 0 46 99 42 696.4 0 42 100 36 696.4 0 36 101 45 696.4 0 45 102 58 696.4 0 58 103 64 696.4 0 64 104 75 696.4 0 75 105 72 696.4 0 72 106 74 696.4 0 74 107 64 696.4 0 64 108 39 696.4 0 39 109 38 696.4 0 38 110 90 696.4 0 90 111 221 696.4 0 221 112 71 696.4 0 71 113 77 696.4 0 77 114 48 696.4 0 48 115 53 696.4 0 53 116 30 696.4 0 30 117 44 696.4 0 44 118 58 696.4 0 58 119 78 696.4 0 78 120 89 696.4 0 89 121 56 696.4 0 56 122 206 696.4 0 206 123 130 696.4 0 130 124 113 696.4 0 113 125 55 696.4 0 55 126 48 696.4 0 48 127 47 696.4 0 47 128 67 696.4 0 67 129 67 696.4 0 67 130 56 696.4 0 56 131 59 696.4 0 59 132 65 696.4 0 65 133 73 696.4 0 73 134 60 696.4 0 60 135 63 696.4 0 63 136 64 696.4 0 64 137 108 696.4 0 108 138 105 696.4 0 105 139 196 696.4 0 196 140 113 696.4 0 113 141 117 696.4 0 117 142 77 696.4 0 77 143 37 696.4 0 37 144 53 696.4 0 53 145 52 696.4 0 52 146 56 696.4 0 56 147 58 696.4 0 58 148 78 696.4 0 78 149 47 696.4 0 47 150 79 696.4 0 79 151 52 696.4 0 52 152 66 696.4 0 66 153 77 696.4 0 77 154 55 696.4 0 55 155 56 696.4 0 56 156 53 696.4 0 53 157 90 696.4 0 90 158 95 696.4 0 95 159 101 696.4 0 101 160 512 696.4 0 512 161 323 696.4 0 323 162 480 696.4 0 480 163 142 696.4 0 142 164 64 696.4 0 64 165 53 696.4 0 53 166 43 696.4 0 43 167 58 696.4 0 58 168 63 696.4 0 63 169 87 696.4 0 87 170 91 696.4 0 91 171 41 696.4 0 41 172 55 696.4 0 55 173 66 696.4 0 66 174 75 696.4 0 75 175 49 696.4 0 49 176 75 696.4 0 75 177 72 696.4 0 72 178 72 696.4 0 72 179 78 696.4 0 78 180 167 696.4 0 167 181 207 696.4 0 207 182 306 696.4 0 306 183 198 696.4 0 198 184 105 696.4 0 105 185 137 696.4 0 137 186 155 696.4 0 155 187 1568 696.4 0 1568 188 2721 696.4 0 2721 189 1275 696.4 0 1275 190 109 696.4 0 109 191 43 696.4 0 43 192 53 696.4 0 53 193 47 696.4 0 47 194 56 696.4 0 56 195 65 696.4 0 65 196 60 696.4 0 60 197 69 696.4 0 69 198 66 696.4 0 66 199 74 696.4 0 74 200 69 696.4 0 69 201 74 696.4 0 74 202 80 696.4 0 80 203 79 696.4 0 79 204 73 696.4 0 73 205 111 696.4 0 111 206 94 696.4 0 94 207 106 696.4 0 106 208 83 696.4 0 83 209 75 696.4 0 75 210 223 696.4 0 223 211 204 696.4 0 204 212 441 696.4 0 441 213 241 696.4 0 241 214 83 696.4 0 83 215 62 696.4 0 62 216 79 696.4 0 79 217 71 696.4 0 71 218 69 696.4 0 69 219 110 696.4 0 110 220 166 696.4 0 166 221 103 696.4 0 103 222 68 696.4 0 68 223 57 696.4 0 57 224 70 696.4 0 70 225 86 696.4 0 86 226 84 696.4 0 84 227 97 696.4 0 97 228 93 696.4 0 93 229 137 696.4 0 137 230 530 696.4 0 530 231 401 696.4 0 401 232 504 696.4 0 504 233 89 696.4 0 89 234 58 696.4 0 58 235 57 696.4 0 57 236 45 696.4 0 45 237 39 696.4 0 39 238 51 696.4 0 51 239 99 696.4 0 99 240 90 696.4 0 90 241 87 696.4 0 87 242 72 696.4 0 72 243 52 696.4 0 52 244 63 696.4 0 63 245 76 696.4 0 76 246 65 696.4 0 65 247 48 696.4 0 48 248 52 696.4 0 52 249 45 696.4 0 45 250 63 696.4 0 63 251 48 696.4 0 48 252 75 696.4 0 75 253 74 696.4 0 74 254 70 696.4 0 70 255 64 696.4 0 64 256 76 696.4 0 76 257 85 696.4 0 85 258 90 696.4 0 90 259 82 696.4 0 82 260 104 696.4 0 104 261 80 696.4 0 80 262 55 696.4 0 55 263 70 696.4 0 70 264 77 696.4 0 77 265 49 696.4 0 49 266 68 696.4 0 68 267 68 696.4 0 68 268 78 696.4 0 78 269 91 696.4 0 91 270 45 696.4 0 45 271 62 696.4 0 62 272 93 696.4 0 93 273 146 696.4 0 146 274 176 696.4 0 176 275 437 696.4 0 437 276 439 696.4 0 439 277 322 696.4 0 322 278 130 696.4 0 130 279 141 696.4 0 141 280 127 696.4 0 127 281 94 696.4 0 94 282 86 696.4 0 86 283 100 696.4 0 100 284 156 696.4 0 156 285 116 696.4 0 116 286 60 696.4 0 60 287 79 696.4 0 79 288 143 696.4 0 143 289 259 696.4 0 259 290 197 696.4 0 197 291 128 696.4 0 128 292 96 696.4 0 96 293 80 696.4 0 80 294 93 696.4 0 93 295 96 696.4 0 96 296 56 696.4 0 56 297 189 696.4 0 189 298 91 696.4 0 91 299 107 696.4 0 107 300 255 696.4 0 255 301 194 696.4 0 194 Finished in 2.61 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 53,060 Reads with adapters: 17,776 (33.5%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 17,757 (33.5%) Total basepairs processed: 12,954,878 bp Total written (filtered): 4,796,157 bp (37.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17776 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 175 0.0 3 0 169 5 1 20 17016 0.0 4 16744 265 7 21 58 0.0 4 12 44 2 22 2 0.0 4 0 0 2 23 8 0.0 4 0 0 0 6 2 24 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 40 1 0.0 4 1 49 1 0.0 4 0 0 0 1 83 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 9 0.0 4 0 0 0 0 9 127 473 0.0 4 0 0 0 0 473 132 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 163 1 0.0 4 1 186 1 0.0 4 0 1 199 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 262 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 17,757 Reads with adapters: 1,562 (8.8%) Reads written (passing filters): 17,757 (100.0%) Total basepairs processed: 4,796,157 bp Total written (filtered): 4,769,724 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1562 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 2.4% G: 91.9% T: 3.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 277.5 0 14 4 5 69.4 0 5 5 1 17.3 0 1 6 98 4.3 0 98 9 27 0.1 0 1 26 10 190 0.0 1 8 182 11 1174 0.0 1 6 1168 12 6 0.0 1 0 6 123 1 0.0 1 1 224 38 0.0 1 35 3 225 3 0.0 1 2 1 265 3 0.0 1 3 272 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.