This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.49 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 114,175 (4.0%) Reads that were too short: 35,234 (1.2%) Reads written (passing filters): 78,941 (2.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 17,422,164 bp (2.0%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 114175 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2261 696.4 0 2261 7 14492 696.4 0 14492 8 2351 696.4 0 2351 9 17706 696.4 0 17706 10 41 696.4 0 41 11 20 696.4 0 20 12 33 696.4 0 33 13 44 696.4 0 44 14 33 696.4 0 33 15 20 696.4 0 20 16 16 696.4 0 16 17 28 696.4 0 28 18 23 696.4 0 23 19 20 696.4 0 20 20 18 696.4 0 18 21 25 696.4 0 25 22 15 696.4 0 15 23 32 696.4 0 32 24 12 696.4 0 12 25 25 696.4 0 25 26 16 696.4 0 16 27 19 696.4 0 19 28 37 696.4 0 37 29 37 696.4 0 37 30 62 696.4 0 62 31 139 696.4 0 139 32 930 696.4 0 930 33 438 696.4 0 438 34 32 696.4 0 32 35 41 696.4 0 41 36 25 696.4 0 25 37 30 696.4 0 30 38 129 696.4 0 129 39 61 696.4 0 61 40 69 696.4 0 69 41 47 696.4 0 47 42 31 696.4 0 31 43 62 696.4 0 62 44 22 696.4 0 22 45 38 696.4 0 38 46 34 696.4 0 34 47 24 696.4 0 24 48 12 696.4 0 12 49 25 696.4 0 25 50 37 696.4 0 37 51 29 696.4 0 29 52 33 696.4 0 33 53 410 696.4 0 410 54 347 696.4 0 347 55 152 696.4 0 152 56 41 696.4 0 41 57 71 696.4 0 71 58 44 696.4 0 44 59 23 696.4 0 23 60 33 696.4 0 33 61 20 696.4 0 20 62 21 696.4 0 21 63 24 696.4 0 24 64 32 696.4 0 32 65 27 696.4 0 27 66 68 696.4 0 68 67 68 696.4 0 68 68 53 696.4 0 53 69 102 696.4 0 102 70 56 696.4 0 56 71 137 696.4 0 137 72 60 696.4 0 60 73 22 696.4 0 22 74 45 696.4 0 45 75 25 696.4 0 25 76 30 696.4 0 30 77 21 696.4 0 21 78 41 696.4 0 41 79 27 696.4 0 27 80 35 696.4 0 35 81 37 696.4 0 37 82 43 696.4 0 43 83 36 696.4 0 36 84 30 696.4 0 30 85 32 696.4 0 32 86 59 696.4 0 59 87 46 696.4 0 46 88 40 696.4 0 40 89 68 696.4 0 68 90 49 696.4 0 49 91 88 696.4 0 88 92 73 696.4 0 73 93 79 696.4 0 79 94 56 696.4 0 56 95 77 696.4 0 77 96 28 696.4 0 28 97 51 696.4 0 51 98 19 696.4 0 19 99 16 696.4 0 16 100 36 696.4 0 36 101 49 696.4 0 49 102 62 696.4 0 62 103 133 696.4 0 133 104 225 696.4 0 225 105 146 696.4 0 146 106 48 696.4 0 48 107 87 696.4 0 87 108 61 696.4 0 61 109 70 696.4 0 70 110 32 696.4 0 32 111 148 696.4 0 148 112 112 696.4 0 112 113 215 696.4 0 215 114 87 696.4 0 87 115 93 696.4 0 93 116 68 696.4 0 68 117 49 696.4 0 49 118 54 696.4 0 54 119 57 696.4 0 57 120 90 696.4 0 90 121 57 696.4 0 57 122 65 696.4 0 65 123 112 696.4 0 112 124 172 696.4 0 172 125 1096 696.4 0 1096 126 1381 696.4 0 1381 127 718 696.4 0 718 128 87 696.4 0 87 129 60 696.4 0 60 130 77 696.4 0 77 131 72 696.4 0 72 132 85 696.4 0 85 133 134 696.4 0 134 134 113 696.4 0 113 135 101 696.4 0 101 136 76 696.4 0 76 137 138 696.4 0 138 138 112 696.4 0 112 139 169 696.4 0 169 140 113 696.4 0 113 141 109 696.4 0 109 142 92 696.4 0 92 143 103 696.4 0 103 144 175 696.4 0 175 145 487 696.4 0 487 146 378 696.4 0 378 147 286 696.4 0 286 148 116 696.4 0 116 149 142 696.4 0 142 150 322 696.4 0 322 151 353 696.4 0 353 152 287 696.4 0 287 153 158 696.4 0 158 154 234 696.4 0 234 155 394 696.4 0 394 156 436 696.4 0 436 157 758 696.4 0 758 158 667 696.4 0 667 159 550 696.4 0 550 160 3966 696.4 0 3966 161 4372 696.4 0 4372 162 3470 696.4 0 3470 163 366 696.4 0 366 164 256 696.4 0 256 165 175 696.4 0 175 166 207 696.4 0 207 167 134 696.4 0 134 168 150 696.4 0 150 169 259 696.4 0 259 170 480 696.4 0 480 171 355 696.4 0 355 172 90 696.4 0 90 173 129 696.4 0 129 174 165 696.4 0 165 175 374 696.4 0 374 176 285 696.4 0 285 177 332 696.4 0 332 178 185 696.4 0 185 179 306 696.4 0 306 180 1500 696.4 0 1500 181 1530 696.4 0 1530 182 1695 696.4 0 1695 183 527 696.4 0 527 184 107 696.4 0 107 185 183 696.4 0 183 186 235 696.4 0 235 187 163 696.4 0 163 188 95 696.4 0 95 189 108 696.4 0 108 190 95 696.4 0 95 191 140 696.4 0 140 192 91 696.4 0 91 193 78 696.4 0 78 194 74 696.4 0 74 195 87 696.4 0 87 196 87 696.4 0 87 197 107 696.4 0 107 198 109 696.4 0 109 199 144 696.4 0 144 200 110 696.4 0 110 201 77 696.4 0 77 202 96 696.4 0 96 203 94 696.4 0 94 204 118 696.4 0 118 205 103 696.4 0 103 206 81 696.4 0 81 207 97 696.4 0 97 208 141 696.4 0 141 209 110 696.4 0 110 210 131 696.4 0 131 211 179 696.4 0 179 212 668 696.4 0 668 213 669 696.4 0 669 214 622 696.4 0 622 215 168 696.4 0 168 216 170 696.4 0 170 217 135 696.4 0 135 218 145 696.4 0 145 219 206 696.4 0 206 220 141 696.4 0 141 221 140 696.4 0 140 222 138 696.4 0 138 223 155 696.4 0 155 224 172 696.4 0 172 225 233 696.4 0 233 226 204 696.4 0 204 227 119 696.4 0 119 228 115 696.4 0 115 229 128 696.4 0 128 230 114 696.4 0 114 231 82 696.4 0 82 232 86 696.4 0 86 233 136 696.4 0 136 234 137 696.4 0 137 235 162 696.4 0 162 236 125 696.4 0 125 237 190 696.4 0 190 238 188 696.4 0 188 239 210 696.4 0 210 240 196 696.4 0 196 241 215 696.4 0 215 242 157 696.4 0 157 243 241 696.4 0 241 244 177 696.4 0 177 245 261 696.4 0 261 246 315 696.4 0 315 247 414 696.4 0 414 248 313 696.4 0 313 249 193 696.4 0 193 250 142 696.4 0 142 251 140 696.4 0 140 252 208 696.4 0 208 253 143 696.4 0 143 254 197 696.4 0 197 255 419 696.4 0 419 256 1141 696.4 0 1141 257 1100 696.4 0 1100 258 877 696.4 0 877 259 441 696.4 0 441 260 240 696.4 0 240 261 200 696.4 0 200 262 204 696.4 0 204 263 181 696.4 0 181 264 169 696.4 0 169 265 171 696.4 0 171 266 530 696.4 0 530 267 585 696.4 0 585 268 920 696.4 0 920 269 287 696.4 0 287 270 232 696.4 0 232 271 336 696.4 0 336 272 2094 696.4 0 2094 273 2959 696.4 0 2959 274 2397 696.4 0 2397 275 2499 696.4 0 2499 276 247 696.4 0 247 277 239 696.4 0 239 278 253 696.4 0 253 279 251 696.4 0 251 280 565 696.4 0 565 281 475 696.4 0 475 282 311 696.4 0 311 283 217 696.4 0 217 284 213 696.4 0 213 285 213 696.4 0 213 286 240 696.4 0 240 287 485 696.4 0 485 288 477 696.4 0 477 289 431 696.4 0 431 290 144 696.4 0 144 291 181 696.4 0 181 292 190 696.4 0 190 293 309 696.4 0 309 294 205 696.4 0 205 295 178 696.4 0 178 296 124 696.4 0 124 297 152 696.4 0 152 298 124 696.4 0 124 299 138 696.4 0 138 300 221 696.4 0 221 301 449 696.4 0 449 Finished in 3.39 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 78,941 Reads with adapters: 20,484 (25.9%) Reads that were too short: 187 (0.2%) Reads written (passing filters): 20,297 (25.7%) Total basepairs processed: 17,422,164 bp Total written (filtered): 5,571,027 bp (32.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20484 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 9 0.0 3 0 0 6 3 16 160 0.0 3 0 134 18 8 17 17936 0.0 3 16909 869 125 33 18 1992 0.0 3 1832 142 14 4 19 40 0.0 3 4 5 19 12 20 63 0.0 3 0 0 2 61 21 9 0.0 3 0 0 0 9 22 2 0.0 3 0 0 0 2 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 6 0.0 3 0 0 0 6 34 6 0.0 3 0 0 0 6 35 9 0.0 3 0 0 2 7 36 5 0.0 3 0 0 0 5 37 8 0.0 3 0 0 1 7 41 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 47 7 0.0 3 0 0 0 7 49 1 0.0 3 0 0 0 1 50 1 0.0 3 1 51 1 0.0 3 1 52 2 0.0 3 1 0 0 1 54 2 0.0 3 0 0 0 2 55 2 0.0 3 0 0 0 2 58 2 0.0 3 0 1 0 1 59 2 0.0 3 1 0 0 1 60 6 0.0 3 2 1 0 3 62 11 0.0 3 0 0 0 11 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 71 12 0.0 3 1 0 0 11 73 2 0.0 3 0 0 1 1 74 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 1 0 0 1 88 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 93 1 0.0 3 1 97 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 110 1 0.0 3 0 1 111 1 0.0 3 0 0 0 1 112 6 0.0 3 0 0 0 6 113 13 0.0 3 0 0 0 13 114 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 122 3 0.0 3 0 0 0 3 123 7 0.0 3 0 0 0 7 125 2 0.0 3 0 0 0 2 126 8 0.0 3 0 0 0 8 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 1 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 1 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 164 2 0.0 3 0 0 0 2 165 3 0.0 3 0 0 0 3 166 2 0.0 3 0 0 0 2 167 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 1 3 196 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 34 0.0 3 0 0 1 33 220 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.72 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 20,297 Reads with adapters: 629 (3.1%) Reads written (passing filters): 20,297 (100.0%) Total basepairs processed: 5,571,027 bp Total written (filtered): 5,569,117 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 629 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.6% C: 16.7% G: 28.9% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 611 317.1 0 611 4 17 79.3 0 17 9 1 0.1 0 1