This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.77 s (28 us/read; 2.17 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 428,286 (15.0%) Reads that were too short: 9,289 (0.3%) Reads written (passing filters): 418,997 (14.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 107,275,640 bp (12.5%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 428286 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4716 696.4 0 4716 7 28432 696.4 0 28432 8 27125 696.4 0 27125 9 203 696.4 0 203 10 24 696.4 0 24 11 43 696.4 0 43 12 233 696.4 0 233 13 19 696.4 0 19 14 19 696.4 0 19 15 33 696.4 0 33 16 40 696.4 0 40 17 45 696.4 0 45 18 33 696.4 0 33 19 61 696.4 0 61 20 60 696.4 0 60 21 116 696.4 0 116 22 412 696.4 0 412 23 123 696.4 0 123 24 270 696.4 0 270 25 187 696.4 0 187 26 93 696.4 0 93 27 208 696.4 0 208 28 109 696.4 0 109 29 245 696.4 0 245 30 277 696.4 0 277 31 205 696.4 0 205 32 194 696.4 0 194 33 325 696.4 0 325 34 663 696.4 0 663 35 845 696.4 0 845 36 1393 696.4 0 1393 37 2715 696.4 0 2715 38 5513 696.4 0 5513 39 72505 696.4 0 72505 40 51986 696.4 0 51986 41 39206 696.4 0 39206 42 325 696.4 0 325 43 187 696.4 0 187 44 288 696.4 0 288 45 586 696.4 0 586 46 1354 696.4 0 1354 47 356 696.4 0 356 48 448 696.4 0 448 49 877 696.4 0 877 50 320 696.4 0 320 51 323 696.4 0 323 52 659 696.4 0 659 53 806 696.4 0 806 54 962 696.4 0 962 55 619 696.4 0 619 56 1153 696.4 0 1153 57 1681 696.4 0 1681 58 2901 696.4 0 2901 59 52801 696.4 0 52801 60 53444 696.4 0 53444 61 50927 696.4 0 50927 62 1721 696.4 0 1721 63 14 696.4 0 14 64 13 696.4 0 13 65 4 696.4 0 4 66 8 696.4 0 8 67 3 696.4 0 3 68 9 696.4 0 9 69 5 696.4 0 5 70 7 696.4 0 7 71 9 696.4 0 9 72 17 696.4 0 17 73 29 696.4 0 29 74 24 696.4 0 24 75 38 696.4 0 38 76 7 696.4 0 7 77 6 696.4 0 6 78 8 696.4 0 8 79 14 696.4 0 14 80 16 696.4 0 16 81 36 696.4 0 36 82 31 696.4 0 31 83 35 696.4 0 35 84 11 696.4 0 11 85 2 696.4 0 2 86 54 696.4 0 54 87 22 696.4 0 22 88 16 696.4 0 16 89 9 696.4 0 9 90 24 696.4 0 24 91 5 696.4 0 5 92 10 696.4 0 10 93 9 696.4 0 9 94 14 696.4 0 14 95 9 696.4 0 9 96 4 696.4 0 4 97 20 696.4 0 20 98 10 696.4 0 10 99 4 696.4 0 4 100 39 696.4 0 39 101 497 696.4 0 497 102 963 696.4 0 963 103 430 696.4 0 430 104 8 696.4 0 8 105 11 696.4 0 11 106 9 696.4 0 9 107 5 696.4 0 5 108 4 696.4 0 4 109 11 696.4 0 11 110 37 696.4 0 37 111 164 696.4 0 164 112 144 696.4 0 144 113 184 696.4 0 184 114 11 696.4 0 11 115 3 696.4 0 3 116 12 696.4 0 12 117 15 696.4 0 15 118 16 696.4 0 16 119 9 696.4 0 9 120 13 696.4 0 13 121 9 696.4 0 9 122 12 696.4 0 12 123 12 696.4 0 12 124 14 696.4 0 14 125 17 696.4 0 17 126 8 696.4 0 8 127 6 696.4 0 6 128 13 696.4 0 13 129 25 696.4 0 25 130 21 696.4 0 21 131 29 696.4 0 29 132 48 696.4 0 48 133 14 696.4 0 14 134 16 696.4 0 16 135 11 696.4 0 11 136 22 696.4 0 22 137 28 696.4 0 28 138 17 696.4 0 17 139 12 696.4 0 12 140 12 696.4 0 12 141 29 696.4 0 29 142 23 696.4 0 23 143 42 696.4 0 42 144 15 696.4 0 15 145 17 696.4 0 17 146 17 696.4 0 17 147 15 696.4 0 15 148 21 696.4 0 21 149 34 696.4 0 34 150 19 696.4 0 19 151 20 696.4 0 20 152 18 696.4 0 18 153 13 696.4 0 13 154 7 696.4 0 7 155 13 696.4 0 13 156 23 696.4 0 23 157 24 696.4 0 24 158 36 696.4 0 36 159 16 696.4 0 16 160 12 696.4 0 12 161 35 696.4 0 35 162 89 696.4 0 89 163 46 696.4 0 46 164 46 696.4 0 46 165 13 696.4 0 13 166 15 696.4 0 15 167 31 696.4 0 31 168 36 696.4 0 36 169 62 696.4 0 62 170 123 696.4 0 123 171 68 696.4 0 68 172 31 696.4 0 31 173 13 696.4 0 13 174 15 696.4 0 15 175 15 696.4 0 15 176 21 696.4 0 21 177 23 696.4 0 23 178 36 696.4 0 36 179 28 696.4 0 28 180 21 696.4 0 21 181 29 696.4 0 29 182 34 696.4 0 34 183 82 696.4 0 82 184 90 696.4 0 90 185 164 696.4 0 164 186 94 696.4 0 94 187 134 696.4 0 134 188 30 696.4 0 30 189 37 696.4 0 37 190 30 696.4 0 30 191 16 696.4 0 16 192 23 696.4 0 23 193 26 696.4 0 26 194 40 696.4 0 40 195 50 696.4 0 50 196 64 696.4 0 64 197 171 696.4 0 171 198 564 696.4 0 564 199 424 696.4 0 424 200 642 696.4 0 642 201 171 696.4 0 171 202 65 696.4 0 65 203 239 696.4 0 239 204 194 696.4 0 194 205 369 696.4 0 369 206 89 696.4 0 89 207 31 696.4 0 31 208 25 696.4 0 25 209 20 696.4 0 20 210 22 696.4 0 22 211 51 696.4 0 51 212 21 696.4 0 21 213 46 696.4 0 46 214 26 696.4 0 26 215 21 696.4 0 21 216 28 696.4 0 28 217 32 696.4 0 32 218 61 696.4 0 61 219 55 696.4 0 55 220 99 696.4 0 99 221 59 696.4 0 59 222 70 696.4 0 70 223 623 696.4 0 623 224 448 696.4 0 448 225 719 696.4 0 719 226 675 696.4 0 675 227 696 696.4 0 696 228 649 696.4 0 649 229 201 696.4 0 201 230 61 696.4 0 61 231 9 696.4 0 9 232 15 696.4 0 15 233 10 696.4 0 10 234 17 696.4 0 17 235 27 696.4 0 27 236 21 696.4 0 21 237 18 696.4 0 18 238 23 696.4 0 23 239 32 696.4 0 32 240 18 696.4 0 18 241 22 696.4 0 22 242 31 696.4 0 31 243 34 696.4 0 34 244 23 696.4 0 23 245 27 696.4 0 27 246 22 696.4 0 22 247 26 696.4 0 26 248 32 696.4 0 32 249 26 696.4 0 26 250 28 696.4 0 28 251 23 696.4 0 23 252 18 696.4 0 18 253 19 696.4 0 19 254 28 696.4 0 28 255 68 696.4 0 68 256 106 696.4 0 106 257 160 696.4 0 160 258 133 696.4 0 133 259 131 696.4 0 131 260 56 696.4 0 56 261 94 696.4 0 94 262 37 696.4 0 37 263 39 696.4 0 39 264 37 696.4 0 37 265 46 696.4 0 46 266 26 696.4 0 26 267 22 696.4 0 22 268 19 696.4 0 19 269 16 696.4 0 16 270 28 696.4 0 28 271 53 696.4 0 53 272 80 696.4 0 80 273 114 696.4 0 114 274 127 696.4 0 127 275 71 696.4 0 71 276 71 696.4 0 71 277 30 696.4 0 30 278 24 696.4 0 24 279 43 696.4 0 43 280 34 696.4 0 34 281 51 696.4 0 51 282 29 696.4 0 29 283 31 696.4 0 31 284 41 696.4 0 41 285 66 696.4 0 66 286 67 696.4 0 67 287 76 696.4 0 76 288 50 696.4 0 50 289 48 696.4 0 48 290 65 696.4 0 65 291 90 696.4 0 90 292 132 696.4 0 132 293 155 696.4 0 155 294 69 696.4 0 69 295 71 696.4 0 71 296 48 696.4 0 48 297 44 696.4 0 44 298 66 696.4 0 66 299 65 696.4 0 65 300 47 696.4 0 47 301 69 696.4 0 69 Finished in 17.20 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 418,997 Reads with adapters: 33,425 (8.0%) Reads that were too short: 483 (0.1%) Reads written (passing filters): 32,942 (7.9%) Total basepairs processed: 107,275,640 bp Total written (filtered): 8,946,538 bp (8.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33425 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 12 0.0 3 0 0 7 5 16 215 0.0 3 0 194 13 8 17 31351 0.0 3 29705 1393 211 42 18 59 0.0 3 6 45 6 2 19 46 0.0 3 5 0 22 19 20 142 0.0 3 1 0 0 141 24 8 0.0 3 0 1 0 7 26 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 14 0.0 3 0 0 0 14 43 3 0.0 3 0 0 0 3 48 3 0.0 3 3 58 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 1 63 5 0.0 3 0 0 1 4 67 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 11 0.0 3 0 0 0 11 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 1 1 103 2 0.0 3 1 0 0 1 104 1 0.0 3 0 0 0 1 107 15 0.0 3 0 0 0 15 109 2 0.0 3 0 0 0 2 110 8 0.0 3 0 0 0 8 112 37 0.0 3 0 0 3 34 113 6 0.0 3 0 0 0 6 114 1 0.0 3 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 1 1 121 1 0.0 3 0 0 1 122 3 0.0 3 0 0 0 3 123 4 0.0 3 0 0 1 3 124 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 126 2 0.0 3 0 0 0 2 127 15 0.0 3 0 0 0 15 128 4 0.0 3 0 0 1 3 129 8 0.0 3 0 0 0 8 130 6 0.0 3 0 0 0 6 131 24 0.0 3 0 0 2 22 132 662 0.0 3 0 0 8 654 133 96 0.0 3 0 0 1 95 134 4 0.0 3 0 0 1 3 135 78 0.0 3 0 0 0 78 136 19 0.0 3 0 0 0 19 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 1 2 149 2 0.0 3 0 0 0 2 150 2 0.0 3 0 0 0 2 151 4 0.0 3 0 0 0 4 153 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 156 4 0.0 3 0 0 0 4 157 2 0.0 3 0 0 0 2 158 18 0.0 3 0 0 0 18 159 9 0.0 3 0 0 0 9 160 40 0.0 3 0 0 0 40 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 167 4 0.0 3 0 0 0 4 170 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 3 0.0 3 1 0 0 2 183 21 0.0 3 0 0 0 21 184 9 0.0 3 0 0 0 9 185 6 0.0 3 0 0 0 6 186 3 0.0 3 0 0 0 3 187 2 0.0 3 0 0 0 2 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 192 12 0.0 3 0 0 0 12 193 5 0.0 3 0 0 0 5 194 1 0.0 3 0 0 0 1 195 6 0.0 3 0 0 0 6 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 207 4 0.0 3 0 0 0 4 208 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 211 4 0.0 3 0 0 0 4 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 224 3 0.0 3 0 0 0 3 226 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 7 0.0 3 0 0 0 7 233 67 0.0 3 0 0 4 63 234 102 0.0 3 0 0 3 99 235 7 0.0 3 0 0 0 7 236 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 3 0.0 3 0 0 0 3 242 5 0.0 3 0 0 0 5 244 3 0.0 3 0 0 0 3 245 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 15 0.0 3 0 0 0 15 248 6 0.0 3 0 0 0 6 249 41 0.0 3 0 0 0 41 250 13 0.0 3 0 0 0 13 253 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 256 3 0.0 3 0 0 0 3 258 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 7 0.0 3 0 0 0 7 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.16 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 32,942 Reads with adapters: 511 (1.6%) Reads written (passing filters): 32,942 (100.0%) Total basepairs processed: 8,946,538 bp Total written (filtered): 8,944,974 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 511 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.7% C: 25.2% G: 19.4% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 492 514.7 0 492 4 10 128.7 0 10 5 7 32.2 0 7 6 1 8.0 0 1 7 1 2.0 0 1