This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.76 s (28 us/read; 2.17 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 493,555 (17.3%) Reads that were too short: 5,539 (0.2%) Reads written (passing filters): 488,016 (17.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 125,842,474 bp (14.7%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 493555 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2197 696.4 0 2197 7 26069 696.4 0 26069 8 34902 696.4 0 34902 9 146 696.4 0 146 10 27 696.4 0 27 11 54 696.4 0 54 12 349 696.4 0 349 13 25 696.4 0 25 14 22 696.4 0 22 15 38 696.4 0 38 16 48 696.4 0 48 17 50 696.4 0 50 18 28 696.4 0 28 19 80 696.4 0 80 20 67 696.4 0 67 21 162 696.4 0 162 22 580 696.4 0 580 23 152 696.4 0 152 24 348 696.4 0 348 25 249 696.4 0 249 26 134 696.4 0 134 27 279 696.4 0 279 28 129 696.4 0 129 29 208 696.4 0 208 30 152 696.4 0 152 31 200 696.4 0 200 32 242 696.4 0 242 33 375 696.4 0 375 34 824 696.4 0 824 35 1056 696.4 0 1056 36 1774 696.4 0 1774 37 3572 696.4 0 3572 38 5477 696.4 0 5477 39 104281 696.4 0 104281 40 75169 696.4 0 75169 41 57327 696.4 0 57327 42 365 696.4 0 365 43 190 696.4 0 190 44 311 696.4 0 311 45 573 696.4 0 573 46 1295 696.4 0 1295 47 331 696.4 0 331 48 412 696.4 0 412 49 838 696.4 0 838 50 274 696.4 0 274 51 303 696.4 0 303 52 621 696.4 0 621 53 735 696.4 0 735 54 818 696.4 0 818 55 551 696.4 0 551 56 1017 696.4 0 1017 57 1333 696.4 0 1333 58 2318 696.4 0 2318 59 49920 696.4 0 49920 60 51637 696.4 0 51637 61 50961 696.4 0 50961 62 1697 696.4 0 1697 63 14 696.4 0 14 64 7 696.4 0 7 65 3 696.4 0 3 66 4 696.4 0 4 67 4 696.4 0 4 68 2 696.4 0 2 69 3 696.4 0 3 70 2 696.4 0 2 71 6 696.4 0 6 72 11 696.4 0 11 73 11 696.4 0 11 74 16 696.4 0 16 75 40 696.4 0 40 76 6 696.4 0 6 77 2 696.4 0 2 78 3 696.4 0 3 79 7 696.4 0 7 80 10 696.4 0 10 81 26 696.4 0 26 82 14 696.4 0 14 83 26 696.4 0 26 84 9 696.4 0 9 85 8 696.4 0 8 86 2 696.4 0 2 87 8 696.4 0 8 88 4 696.4 0 4 89 5 696.4 0 5 90 16 696.4 0 16 91 15 696.4 0 15 92 18 696.4 0 18 93 6 696.4 0 6 94 5 696.4 0 5 95 8 696.4 0 8 96 10 696.4 0 10 97 11 696.4 0 11 98 19 696.4 0 19 99 9 696.4 0 9 100 21 696.4 0 21 101 300 696.4 0 300 102 523 696.4 0 523 103 216 696.4 0 216 104 8 696.4 0 8 105 10 696.4 0 10 106 6 696.4 0 6 107 6 696.4 0 6 108 6 696.4 0 6 109 14 696.4 0 14 110 21 696.4 0 21 111 133 696.4 0 133 112 151 696.4 0 151 113 223 696.4 0 223 114 7 696.4 0 7 115 7 696.4 0 7 116 7 696.4 0 7 117 12 696.4 0 12 118 11 696.4 0 11 119 12 696.4 0 12 120 9 696.4 0 9 121 7 696.4 0 7 122 7 696.4 0 7 123 8 696.4 0 8 124 11 696.4 0 11 125 14 696.4 0 14 126 5 696.4 0 5 127 6 696.4 0 6 128 4 696.4 0 4 129 12 696.4 0 12 130 16 696.4 0 16 131 28 696.4 0 28 132 29 696.4 0 29 133 13 696.4 0 13 134 10 696.4 0 10 135 5 696.4 0 5 136 23 696.4 0 23 137 15 696.4 0 15 138 5 696.4 0 5 139 11 696.4 0 11 140 4 696.4 0 4 141 13 696.4 0 13 142 13 696.4 0 13 143 23 696.4 0 23 144 3 696.4 0 3 145 10 696.4 0 10 146 18 696.4 0 18 147 4 696.4 0 4 148 23 696.4 0 23 149 27 696.4 0 27 150 21 696.4 0 21 151 21 696.4 0 21 152 18 696.4 0 18 153 8 696.4 0 8 154 8 696.4 0 8 155 7 696.4 0 7 156 14 696.4 0 14 157 19 696.4 0 19 158 24 696.4 0 24 159 10 696.4 0 10 160 10 696.4 0 10 161 20 696.4 0 20 162 35 696.4 0 35 163 27 696.4 0 27 164 16 696.4 0 16 165 9 696.4 0 9 166 9 696.4 0 9 167 24 696.4 0 24 168 29 696.4 0 29 169 39 696.4 0 39 170 152 696.4 0 152 171 60 696.4 0 60 172 17 696.4 0 17 173 10 696.4 0 10 174 3 696.4 0 3 175 8 696.4 0 8 176 13 696.4 0 13 177 13 696.4 0 13 178 24 696.4 0 24 179 13 696.4 0 13 180 20 696.4 0 20 181 6 696.4 0 6 182 10 696.4 0 10 183 27 696.4 0 27 184 52 696.4 0 52 185 105 696.4 0 105 186 75 696.4 0 75 187 135 696.4 0 135 188 20 696.4 0 20 189 32 696.4 0 32 190 13 696.4 0 13 191 8 696.4 0 8 192 6 696.4 0 6 193 17 696.4 0 17 194 23 696.4 0 23 195 23 696.4 0 23 196 29 696.4 0 29 197 124 696.4 0 124 198 332 696.4 0 332 199 197 696.4 0 197 200 288 696.4 0 288 201 61 696.4 0 61 202 33 696.4 0 33 203 50 696.4 0 50 204 47 696.4 0 47 205 75 696.4 0 75 206 28 696.4 0 28 207 12 696.4 0 12 208 8 696.4 0 8 209 22 696.4 0 22 210 13 696.4 0 13 211 32 696.4 0 32 212 12 696.4 0 12 213 20 696.4 0 20 214 24 696.4 0 24 215 13 696.4 0 13 216 14 696.4 0 14 217 17 696.4 0 17 218 33 696.4 0 33 219 29 696.4 0 29 220 44 696.4 0 44 221 29 696.4 0 29 222 30 696.4 0 30 223 219 696.4 0 219 224 168 696.4 0 168 225 284 696.4 0 284 226 636 696.4 0 636 227 775 696.4 0 775 228 871 696.4 0 871 229 237 696.4 0 237 230 38 696.4 0 38 231 7 696.4 0 7 232 4 696.4 0 4 233 9 696.4 0 9 234 8 696.4 0 8 235 12 696.4 0 12 236 14 696.4 0 14 237 12 696.4 0 12 238 16 696.4 0 16 239 14 696.4 0 14 240 15 696.4 0 15 241 12 696.4 0 12 242 11 696.4 0 11 243 21 696.4 0 21 244 11 696.4 0 11 245 14 696.4 0 14 246 14 696.4 0 14 247 20 696.4 0 20 248 23 696.4 0 23 249 23 696.4 0 23 250 10 696.4 0 10 251 13 696.4 0 13 252 19 696.4 0 19 253 11 696.4 0 11 254 8 696.4 0 8 255 29 696.4 0 29 256 45 696.4 0 45 257 78 696.4 0 78 258 54 696.4 0 54 259 34 696.4 0 34 260 21 696.4 0 21 261 33 696.4 0 33 262 19 696.4 0 19 263 22 696.4 0 22 264 28 696.4 0 28 265 24 696.4 0 24 266 9 696.4 0 9 267 12 696.4 0 12 268 17 696.4 0 17 269 17 696.4 0 17 270 16 696.4 0 16 271 34 696.4 0 34 272 19 696.4 0 19 273 39 696.4 0 39 274 59 696.4 0 59 275 43 696.4 0 43 276 54 696.4 0 54 277 16 696.4 0 16 278 14 696.4 0 14 279 15 696.4 0 15 280 11 696.4 0 11 281 24 696.4 0 24 282 15 696.4 0 15 283 22 696.4 0 22 284 27 696.4 0 27 285 37 696.4 0 37 286 24 696.4 0 24 287 30 696.4 0 30 288 19 696.4 0 19 289 25 696.4 0 25 290 32 696.4 0 32 291 40 696.4 0 40 292 51 696.4 0 51 293 78 696.4 0 78 294 33 696.4 0 33 295 27 696.4 0 27 296 11 696.4 0 11 297 20 696.4 0 20 298 31 696.4 0 31 299 28 696.4 0 28 300 28 696.4 0 28 301 41 696.4 0 41 Finished in 22.96 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 488,016 Reads with adapters: 51,292 (10.5%) Reads that were too short: 123 (0.0%) Reads written (passing filters): 51,169 (10.5%) Total basepairs processed: 125,842,474 bp Total written (filtered): 12,363,239 bp (9.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 51292 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 155 0.0 3 0 149 4 2 20 35278 0.0 4 34719 546 12 1 21 94 0.0 4 9 85 22 9 0.0 4 1 3 5 23 517 0.0 4 469 6 1 36 5 24 2 0.0 4 0 1 0 0 1 27 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 1 73 23 0.0 4 0 0 0 0 23 74 31 0.0 4 0 0 0 0 31 75 14785 0.0 4 0 0 0 0 14785 76 1 0.0 4 0 0 0 0 1 78 8 0.0 4 0 0 0 0 8 82 1 0.0 4 0 0 0 1 83 4 0.0 4 0 0 0 0 4 87 1 0.0 4 1 88 2 0.0 4 0 1 0 0 1 101 1 0.0 4 1 103 1 0.0 4 1 106 2 0.0 4 2 110 1 0.0 4 1 114 1 0.0 4 1 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 127 238 0.0 4 0 0 0 0 238 131 1 0.0 4 0 0 0 0 1 135 2 0.0 4 1 0 0 1 140 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 158 3 0.0 4 0 0 0 0 3 169 3 0.0 4 0 0 0 0 3 170 3 0.0 4 0 0 0 0 3 171 1 0.0 4 0 0 0 1 176 55 0.0 4 0 0 0 1 54 177 8 0.0 4 0 0 0 0 8 178 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 182 3 0.0 4 0 0 0 1 2 183 6 0.0 4 0 0 0 0 6 184 16 0.0 4 0 0 0 0 16 186 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 1 216 1 0.0 4 1 228 3 0.0 4 0 0 0 0 3 230 3 0.0 4 0 0 0 0 3 236 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 260 1 0.0 4 1 277 1 0.0 4 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.66 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 51,169 Reads with adapters: 3,259 (6.4%) Reads written (passing filters): 51,169 (100.0%) Total basepairs processed: 12,363,239 bp Total written (filtered): 12,251,949 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3259 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 20.0% C: 3.0% G: 75.6% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 52 799.5 0 52 4 111 199.9 0 111 5 2 50.0 0 2 6 1 12.5 0 1 9 69 0.2 0 5 64 10 658 0.0 1 22 636 11 1860 0.0 1 12 1848 12 1 0.0 1 0 1 13 2 0.0 1 0 2 14 2 0.0 1 1 1 18 2 0.0 1 0 2 19 157 0.0 1 131 26 20 10 0.0 1 9 1 21 1 0.0 1 1 22 1 0.0 1 1 23 17 0.0 1 13 4 49 6 0.0 1 4 2 81 1 0.0 1 0 1 209 6 0.0 1 1 5 221 1 0.0 1 0 1 258 4 0.0 1 4 259 290 0.0 1 265 25 260 3 0.0 1 3 261 2 0.0 1 2