This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.85 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 303,231 (10.6%) Reads that were too short: 34,772 (1.2%) Reads written (passing filters): 268,459 (9.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 76,800,490 bp (8.9%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 303231 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9372 696.4 0 9372 7 115087 696.4 0 115087 8 2142 696.4 0 2142 9 124194 696.4 0 124194 10 125 696.4 0 125 11 78 696.4 0 78 12 45 696.4 0 45 13 7 696.4 0 7 14 10 696.4 0 10 15 9 696.4 0 9 16 6 696.4 0 6 17 20 696.4 0 20 18 4 696.4 0 4 19 8 696.4 0 8 20 8 696.4 0 8 21 3 696.4 0 3 22 13 696.4 0 13 23 12 696.4 0 12 24 26 696.4 0 26 25 14 696.4 0 14 26 14 696.4 0 14 27 51 696.4 0 51 28 60 696.4 0 60 29 271 696.4 0 271 30 11 696.4 0 11 31 6 696.4 0 6 32 23 696.4 0 23 33 3 696.4 0 3 34 13 696.4 0 13 35 22 696.4 0 22 36 44 696.4 0 44 37 35 696.4 0 35 38 24 696.4 0 24 39 18 696.4 0 18 40 11 696.4 0 11 41 11 696.4 0 11 42 40 696.4 0 40 43 2 696.4 0 2 44 13 696.4 0 13 45 23 696.4 0 23 46 19 696.4 0 19 47 13 696.4 0 13 48 24 696.4 0 24 49 12 696.4 0 12 50 15 696.4 0 15 51 43 696.4 0 43 52 33 696.4 0 33 53 35 696.4 0 35 54 28 696.4 0 28 55 30 696.4 0 30 56 7 696.4 0 7 57 15 696.4 0 15 58 16 696.4 0 16 59 19 696.4 0 19 60 39 696.4 0 39 61 133 696.4 0 133 62 1012 696.4 0 1012 63 404 696.4 0 404 64 411 696.4 0 411 65 45 696.4 0 45 66 62 696.4 0 62 67 37 696.4 0 37 68 27 696.4 0 27 69 35 696.4 0 35 70 27 696.4 0 27 71 22 696.4 0 22 72 56 696.4 0 56 73 120 696.4 0 120 74 104 696.4 0 104 75 59 696.4 0 59 76 81 696.4 0 81 77 104 696.4 0 104 78 1326 696.4 0 1326 79 1556 696.4 0 1556 80 1110 696.4 0 1110 81 82 696.4 0 82 82 235 696.4 0 235 83 204 696.4 0 204 84 24 696.4 0 24 85 44 696.4 0 44 86 49 696.4 0 49 87 39 696.4 0 39 88 16 696.4 0 16 89 11 696.4 0 11 90 35 696.4 0 35 91 17 696.4 0 17 92 46 696.4 0 46 93 64 696.4 0 64 94 24 696.4 0 24 95 15 696.4 0 15 96 26 696.4 0 26 97 9 696.4 0 9 98 13 696.4 0 13 99 10 696.4 0 10 100 12 696.4 0 12 101 19 696.4 0 19 102 19 696.4 0 19 103 14 696.4 0 14 104 14 696.4 0 14 105 13 696.4 0 13 106 10 696.4 0 10 107 15 696.4 0 15 108 16 696.4 0 16 109 57 696.4 0 57 110 42 696.4 0 42 111 42 696.4 0 42 112 20 696.4 0 20 113 25 696.4 0 25 114 28 696.4 0 28 115 123 696.4 0 123 116 127 696.4 0 127 117 158 696.4 0 158 118 55 696.4 0 55 119 22 696.4 0 22 120 46 696.4 0 46 121 51 696.4 0 51 122 56 696.4 0 56 123 100 696.4 0 100 124 51 696.4 0 51 125 132 696.4 0 132 126 78 696.4 0 78 127 81 696.4 0 81 128 37 696.4 0 37 129 61 696.4 0 61 130 62 696.4 0 62 131 77 696.4 0 77 132 182 696.4 0 182 133 24 696.4 0 24 134 22 696.4 0 22 135 39 696.4 0 39 136 37 696.4 0 37 137 53 696.4 0 53 138 28 696.4 0 28 139 30 696.4 0 30 140 43 696.4 0 43 141 43 696.4 0 43 142 75 696.4 0 75 143 65 696.4 0 65 144 259 696.4 0 259 145 175 696.4 0 175 146 179 696.4 0 179 147 49 696.4 0 49 148 51 696.4 0 51 149 50 696.4 0 50 150 55 696.4 0 55 151 49 696.4 0 49 152 50 696.4 0 50 153 47 696.4 0 47 154 39 696.4 0 39 155 56 696.4 0 56 156 59 696.4 0 59 157 49 696.4 0 49 158 69 696.4 0 69 159 32 696.4 0 32 160 46 696.4 0 46 161 41 696.4 0 41 162 58 696.4 0 58 163 45 696.4 0 45 164 57 696.4 0 57 165 72 696.4 0 72 166 92 696.4 0 92 167 82 696.4 0 82 168 66 696.4 0 66 169 207 696.4 0 207 170 64 696.4 0 64 171 59 696.4 0 59 172 61 696.4 0 61 173 51 696.4 0 51 174 61 696.4 0 61 175 57 696.4 0 57 176 61 696.4 0 61 177 45 696.4 0 45 178 75 696.4 0 75 179 77 696.4 0 77 180 99 696.4 0 99 181 335 696.4 0 335 182 269 696.4 0 269 183 634 696.4 0 634 184 63 696.4 0 63 185 124 696.4 0 124 186 185 696.4 0 185 187 168 696.4 0 168 188 86 696.4 0 86 189 76 696.4 0 76 190 172 696.4 0 172 191 147 696.4 0 147 192 213 696.4 0 213 193 104 696.4 0 104 194 109 696.4 0 109 195 80 696.4 0 80 196 132 696.4 0 132 197 129 696.4 0 129 198 195 696.4 0 195 199 125 696.4 0 125 200 82 696.4 0 82 201 63 696.4 0 63 202 53 696.4 0 53 203 79 696.4 0 79 204 142 696.4 0 142 205 109 696.4 0 109 206 95 696.4 0 95 207 60 696.4 0 60 208 87 696.4 0 87 209 146 696.4 0 146 210 155 696.4 0 155 211 135 696.4 0 135 212 139 696.4 0 139 213 155 696.4 0 155 214 162 696.4 0 162 215 175 696.4 0 175 216 227 696.4 0 227 217 1079 696.4 0 1079 218 810 696.4 0 810 219 681 696.4 0 681 220 209 696.4 0 209 221 198 696.4 0 198 222 249 696.4 0 249 223 216 696.4 0 216 224 346 696.4 0 346 225 3352 696.4 0 3352 226 2528 696.4 0 2528 227 4738 696.4 0 4738 228 754 696.4 0 754 229 504 696.4 0 504 230 161 696.4 0 161 231 115 696.4 0 115 232 93 696.4 0 93 233 158 696.4 0 158 234 123 696.4 0 123 235 167 696.4 0 167 236 161 696.4 0 161 237 707 696.4 0 707 238 1010 696.4 0 1010 239 825 696.4 0 825 240 537 696.4 0 537 241 155 696.4 0 155 242 180 696.4 0 180 243 127 696.4 0 127 244 130 696.4 0 130 245 431 696.4 0 431 246 293 696.4 0 293 247 364 696.4 0 364 248 215 696.4 0 215 249 154 696.4 0 154 250 141 696.4 0 141 251 82 696.4 0 82 252 74 696.4 0 74 253 74 696.4 0 74 254 111 696.4 0 111 255 130 696.4 0 130 256 111 696.4 0 111 257 94 696.4 0 94 258 79 696.4 0 79 259 78 696.4 0 78 260 387 696.4 0 387 261 283 696.4 0 283 262 395 696.4 0 395 263 195 696.4 0 195 264 179 696.4 0 179 265 248 696.4 0 248 266 250 696.4 0 250 267 349 696.4 0 349 268 150 696.4 0 150 269 82 696.4 0 82 270 76 696.4 0 76 271 117 696.4 0 117 272 138 696.4 0 138 273 236 696.4 0 236 274 156 696.4 0 156 275 93 696.4 0 93 276 105 696.4 0 105 277 256 696.4 0 256 278 321 696.4 0 321 279 396 696.4 0 396 280 235 696.4 0 235 281 174 696.4 0 174 282 136 696.4 0 136 283 91 696.4 0 91 284 114 696.4 0 114 285 313 696.4 0 313 286 550 696.4 0 550 287 989 696.4 0 989 288 566 696.4 0 566 289 153 696.4 0 153 290 165 696.4 0 165 291 192 696.4 0 192 292 174 696.4 0 174 293 130 696.4 0 130 294 166 696.4 0 166 295 168 696.4 0 168 296 195 696.4 0 195 297 176 696.4 0 176 298 171 696.4 0 171 299 264 696.4 0 264 300 263 696.4 0 263 301 312 696.4 0 312 Finished in 12.14 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 268,459 Reads with adapters: 128,701 (47.9%) Reads that were too short: 181 (0.1%) Reads written (passing filters): 128,520 (47.9%) Total basepairs processed: 76,800,490 bp Total written (filtered): 35,322,873 bp (46.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 128701 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 15 0.0 2 0 0 0 15 15 58 0.0 3 0 0 35 23 16 739 0.0 3 0 601 100 38 17 126741 0.0 3 118100 7061 1285 295 18 368 0.0 3 19 273 24 52 19 218 0.0 3 2 1 127 88 20 199 0.0 3 0 0 2 197 21 7 0.0 3 0 0 0 7 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 22 0.0 3 0 0 1 21 28 1 0.0 3 0 0 0 1 30 3 0.0 3 1 0 0 2 32 1 0.0 3 0 0 0 1 34 1 0.0 3 1 36 1 0.0 3 1 39 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 0 4 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 68 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 1 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 107 4 0.0 3 0 0 0 4 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 2 0.0 3 1 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 1 0 1 118 3 0.0 3 0 0 0 3 120 1 0.0 3 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 128 1 0.0 3 0 1 129 11 0.0 3 0 0 0 11 130 17 0.0 3 0 0 0 17 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 6 0.0 3 0 0 0 6 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 157 6 0.0 3 0 0 0 6 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 5 0.0 3 0 0 0 5 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 1 1 188 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 12 0.0 3 0 0 2 10 205 5 0.0 3 0 0 0 5 206 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 209 5 0.0 3 0 0 0 5 210 2 0.0 3 0 0 0 2 211 2 0.0 3 0 0 0 2 214 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 6 0.0 3 0 0 1 5 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 231 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 1 274 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 9 0.0 3 0 0 0 9 280 15 0.0 3 0 0 0 15 281 12 0.0 3 0 0 1 11 282 24 0.0 3 0 0 1 23 283 10 0.0 3 0 0 0 10 284 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 293 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.85 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 128,520 Reads with adapters: 16,145 (12.6%) Reads written (passing filters): 128,520 (100.0%) Total basepairs processed: 35,322,873 bp Total written (filtered): 35,274,135 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 16145 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.0% C: 14.4% G: 16.9% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 16023 2008.1 0 16023 4 86 502.0 0 86 5 4 125.5 0 4 9 20 0.5 0 0 20 10 9 0.1 1 0 9 11 2 0.0 1 0 2 13 1 0.0 1 0 1