This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.05 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 284,673 (10.0%) Reads that were too short: 16,361 (0.6%) Reads written (passing filters): 268,312 (9.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 77,573,575 bp (9.0%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 284673 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3685 696.4 0 3685 7 133706 696.4 0 133706 8 1605 696.4 0 1605 9 119409 696.4 0 119409 10 74 696.4 0 74 11 125 696.4 0 125 12 20 696.4 0 20 13 3 696.4 0 3 14 7 696.4 0 7 15 8 696.4 0 8 16 3 696.4 0 3 17 9 696.4 0 9 18 3 696.4 0 3 19 2 696.4 0 2 20 5 696.4 0 5 21 9 696.4 0 9 22 10 696.4 0 10 23 6 696.4 0 6 24 9 696.4 0 9 25 8 696.4 0 8 26 4 696.4 0 4 27 47 696.4 0 47 28 51 696.4 0 51 29 192 696.4 0 192 30 5 696.4 0 5 31 12 696.4 0 12 32 20 696.4 0 20 33 4 696.4 0 4 34 12 696.4 0 12 35 8 696.4 0 8 36 60 696.4 0 60 37 75 696.4 0 75 38 27 696.4 0 27 39 8 696.4 0 8 40 11 696.4 0 11 41 14 696.4 0 14 42 45 696.4 0 45 43 9 696.4 0 9 44 9 696.4 0 9 45 10 696.4 0 10 46 10 696.4 0 10 47 15 696.4 0 15 48 19 696.4 0 19 49 8 696.4 0 8 50 13 696.4 0 13 51 27 696.4 0 27 52 30 696.4 0 30 53 8 696.4 0 8 54 17 696.4 0 17 55 15 696.4 0 15 56 10 696.4 0 10 57 7 696.4 0 7 58 10 696.4 0 10 59 10 696.4 0 10 60 18 696.4 0 18 61 38 696.4 0 38 62 466 696.4 0 466 63 215 696.4 0 215 64 197 696.4 0 197 65 19 696.4 0 19 66 22 696.4 0 22 67 10 696.4 0 10 68 13 696.4 0 13 69 13 696.4 0 13 70 12 696.4 0 12 71 8 696.4 0 8 72 11 696.4 0 11 73 31 696.4 0 31 74 26 696.4 0 26 75 45 696.4 0 45 76 23 696.4 0 23 77 39 696.4 0 39 78 514 696.4 0 514 79 840 696.4 0 840 80 596 696.4 0 596 81 27 696.4 0 27 82 97 696.4 0 97 83 117 696.4 0 117 84 12 696.4 0 12 85 27 696.4 0 27 86 22 696.4 0 22 87 10 696.4 0 10 88 4 696.4 0 4 89 15 696.4 0 15 90 18 696.4 0 18 91 27 696.4 0 27 92 29 696.4 0 29 93 24 696.4 0 24 94 26 696.4 0 26 95 25 696.4 0 25 96 25 696.4 0 25 97 12 696.4 0 12 98 14 696.4 0 14 99 17 696.4 0 17 100 15 696.4 0 15 101 17 696.4 0 17 102 20 696.4 0 20 103 13 696.4 0 13 104 13 696.4 0 13 105 5 696.4 0 5 106 6 696.4 0 6 107 10 696.4 0 10 108 19 696.4 0 19 109 38 696.4 0 38 110 29 696.4 0 29 111 32 696.4 0 32 112 20 696.4 0 20 113 16 696.4 0 16 114 9 696.4 0 9 115 33 696.4 0 33 116 42 696.4 0 42 117 81 696.4 0 81 118 42 696.4 0 42 119 12 696.4 0 12 120 23 696.4 0 23 121 36 696.4 0 36 122 25 696.4 0 25 123 52 696.4 0 52 124 28 696.4 0 28 125 60 696.4 0 60 126 36 696.4 0 36 127 47 696.4 0 47 128 45 696.4 0 45 129 30 696.4 0 30 130 35 696.4 0 35 131 25 696.4 0 25 132 185 696.4 0 185 133 16 696.4 0 16 134 17 696.4 0 17 135 22 696.4 0 22 136 24 696.4 0 24 137 29 696.4 0 29 138 24 696.4 0 24 139 18 696.4 0 18 140 18 696.4 0 18 141 29 696.4 0 29 142 53 696.4 0 53 143 34 696.4 0 34 144 94 696.4 0 94 145 56 696.4 0 56 146 72 696.4 0 72 147 24 696.4 0 24 148 25 696.4 0 25 149 21 696.4 0 21 150 25 696.4 0 25 151 23 696.4 0 23 152 33 696.4 0 33 153 17 696.4 0 17 154 19 696.4 0 19 155 29 696.4 0 29 156 34 696.4 0 34 157 35 696.4 0 35 158 38 696.4 0 38 159 30 696.4 0 30 160 25 696.4 0 25 161 18 696.4 0 18 162 22 696.4 0 22 163 27 696.4 0 27 164 21 696.4 0 21 165 25 696.4 0 25 166 44 696.4 0 44 167 33 696.4 0 33 168 25 696.4 0 25 169 177 696.4 0 177 170 33 696.4 0 33 171 27 696.4 0 27 172 36 696.4 0 36 173 20 696.4 0 20 174 29 696.4 0 29 175 39 696.4 0 39 176 30 696.4 0 30 177 34 696.4 0 34 178 32 696.4 0 32 179 45 696.4 0 45 180 61 696.4 0 61 181 387 696.4 0 387 182 295 696.4 0 295 183 659 696.4 0 659 184 35 696.4 0 35 185 44 696.4 0 44 186 60 696.4 0 60 187 90 696.4 0 90 188 51 696.4 0 51 189 42 696.4 0 42 190 69 696.4 0 69 191 94 696.4 0 94 192 121 696.4 0 121 193 59 696.4 0 59 194 48 696.4 0 48 195 35 696.4 0 35 196 44 696.4 0 44 197 34 696.4 0 34 198 38 696.4 0 38 199 49 696.4 0 49 200 27 696.4 0 27 201 38 696.4 0 38 202 29 696.4 0 29 203 41 696.4 0 41 204 66 696.4 0 66 205 47 696.4 0 47 206 35 696.4 0 35 207 29 696.4 0 29 208 42 696.4 0 42 209 53 696.4 0 53 210 63 696.4 0 63 211 66 696.4 0 66 212 69 696.4 0 69 213 175 696.4 0 175 214 124 696.4 0 124 215 137 696.4 0 137 216 77 696.4 0 77 217 602 696.4 0 602 218 432 696.4 0 432 219 380 696.4 0 380 220 81 696.4 0 81 221 87 696.4 0 87 222 111 696.4 0 111 223 91 696.4 0 91 224 135 696.4 0 135 225 1471 696.4 0 1471 226 1031 696.4 0 1031 227 2078 696.4 0 2078 228 295 696.4 0 295 229 227 696.4 0 227 230 90 696.4 0 90 231 54 696.4 0 54 232 58 696.4 0 58 233 89 696.4 0 89 234 63 696.4 0 63 235 74 696.4 0 74 236 68 696.4 0 68 237 327 696.4 0 327 238 496 696.4 0 496 239 329 696.4 0 329 240 143 696.4 0 143 241 82 696.4 0 82 242 81 696.4 0 81 243 75 696.4 0 75 244 57 696.4 0 57 245 132 696.4 0 132 246 109 696.4 0 109 247 95 696.4 0 95 248 79 696.4 0 79 249 57 696.4 0 57 250 75 696.4 0 75 251 41 696.4 0 41 252 23 696.4 0 23 253 52 696.4 0 52 254 40 696.4 0 40 255 47 696.4 0 47 256 37 696.4 0 37 257 44 696.4 0 44 258 40 696.4 0 40 259 61 696.4 0 61 260 375 696.4 0 375 261 284 696.4 0 284 262 346 696.4 0 346 263 136 696.4 0 136 264 120 696.4 0 120 265 113 696.4 0 113 266 104 696.4 0 104 267 151 696.4 0 151 268 41 696.4 0 41 269 51 696.4 0 51 270 42 696.4 0 42 271 50 696.4 0 50 272 59 696.4 0 59 273 195 696.4 0 195 274 99 696.4 0 99 275 39 696.4 0 39 276 30 696.4 0 30 277 137 696.4 0 137 278 156 696.4 0 156 279 210 696.4 0 210 280 87 696.4 0 87 281 80 696.4 0 80 282 65 696.4 0 65 283 53 696.4 0 53 284 54 696.4 0 54 285 126 696.4 0 126 286 300 696.4 0 300 287 465 696.4 0 465 288 272 696.4 0 272 289 92 696.4 0 92 290 71 696.4 0 71 291 80 696.4 0 80 292 72 696.4 0 72 293 62 696.4 0 62 294 76 696.4 0 76 295 64 696.4 0 64 296 90 696.4 0 90 297 85 696.4 0 85 298 67 696.4 0 67 299 86 696.4 0 86 300 78 696.4 0 78 301 106 696.4 0 106 Finished in 13.08 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 268,312 Reads with adapters: 136,627 (50.9%) Reads that were too short: 66 (0.0%) Reads written (passing filters): 136,561 (50.9%) Total basepairs processed: 77,573,575 bp Total written (filtered): 37,302,150 bp (48.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 136627 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 14 0.0 3 0 0 12 1 1 19 366 0.0 3 0 349 7 5 5 20 134714 0.0 4 132373 2267 67 6 1 21 266 0.0 4 35 227 4 22 20 0.0 4 2 0 18 23 87 0.0 4 2 0 1 72 12 24 7 0.0 4 0 0 0 0 7 26 1 0.0 4 0 0 0 1 27 3 0.0 4 0 0 0 0 3 29 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 41 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 65 1 0.0 4 1 84 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 1 118 1 0.0 4 0 0 1 123 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 1062 0.0 4 0 0 0 0 1062 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 1 195 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 12 0.0 4 0 0 0 0 12 231 1 0.0 4 0 0 0 0 1 232 17 0.0 4 0 0 0 0 17 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 4 0.0 4 0 0 0 0 4 264 3 0.0 4 0 0 0 0 3 265 3 0.0 4 0 0 0 1 2 267 1 0.0 4 0 0 0 1 276 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.70 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 136,561 Reads with adapters: 5,212 (3.8%) Reads written (passing filters): 136,561 (100.0%) Total basepairs processed: 37,302,150 bp Total written (filtered): 37,228,810 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5212 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.8% C: 4.2% G: 84.7% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 185 2133.8 0 185 4 22 533.4 0 22 5 4 133.4 0 4 6 1 33.3 0 1 9 10 0.5 0 2 8 10 44 0.1 1 0 44 11 4565 0.0 1 37 4528 12 92 0.0 1 4 88 13 4 0.0 1 0 4 14 1 0.0 1 0 1 15 4 0.0 1 2 2 20 176 0.0 1 138 38 21 6 0.0 1 3 3 24 12 0.0 1 8 4 25 2 0.0 1 1 1 79 3 0.0 1 3 83 1 0.0 1 1 89 1 0.0 1 1 93 1 0.0 1 1 152 2 0.0 1 0 2 171 1 0.0 1 1 179 1 0.0 1 0 1 210 72 0.0 1 62 10 211 1 0.0 1 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.