This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.74 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 112,427 (3.9%) Reads that were too short: 14,957 (0.5%) Reads written (passing filters): 97,470 (3.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,895,118 bp (2.8%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 112427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 394 696.4 0 394 7 740 696.4 0 740 8 25957 696.4 0 25957 9 22714 696.4 0 22714 10 45 696.4 0 45 11 80 696.4 0 80 12 77 696.4 0 77 13 143 696.4 0 143 14 32 696.4 0 32 15 25 696.4 0 25 16 20 696.4 0 20 17 49 696.4 0 49 18 64 696.4 0 64 19 27 696.4 0 27 20 36 696.4 0 36 21 29 696.4 0 29 22 50 696.4 0 50 23 35 696.4 0 35 24 30 696.4 0 30 25 67 696.4 0 67 26 70 696.4 0 70 27 43 696.4 0 43 28 35 696.4 0 35 29 30 696.4 0 30 30 66 696.4 0 66 31 415 696.4 0 415 32 572 696.4 0 572 33 210 696.4 0 210 34 38 696.4 0 38 35 51 696.4 0 51 36 25 696.4 0 25 37 56 696.4 0 56 38 63 696.4 0 63 39 60 696.4 0 60 40 43 696.4 0 43 41 57 696.4 0 57 42 76 696.4 0 76 43 59 696.4 0 59 44 37 696.4 0 37 45 35 696.4 0 35 46 58 696.4 0 58 47 55 696.4 0 55 48 111 696.4 0 111 49 62 696.4 0 62 50 54 696.4 0 54 51 132 696.4 0 132 52 67 696.4 0 67 53 132 696.4 0 132 54 44 696.4 0 44 55 68 696.4 0 68 56 100 696.4 0 100 57 92 696.4 0 92 58 43 696.4 0 43 59 31 696.4 0 31 60 54 696.4 0 54 61 41 696.4 0 41 62 46 696.4 0 46 63 31 696.4 0 31 64 45 696.4 0 45 65 50 696.4 0 50 66 111 696.4 0 111 67 72 696.4 0 72 68 44 696.4 0 44 69 52 696.4 0 52 70 83 696.4 0 83 71 46 696.4 0 46 72 61 696.4 0 61 73 47 696.4 0 47 74 57 696.4 0 57 75 63 696.4 0 63 76 109 696.4 0 109 77 75 696.4 0 75 78 43 696.4 0 43 79 34 696.4 0 34 80 34 696.4 0 34 81 72 696.4 0 72 82 107 696.4 0 107 83 98 696.4 0 98 84 85 696.4 0 85 85 192 696.4 0 192 86 1171 696.4 0 1171 87 544 696.4 0 544 88 525 696.4 0 525 89 151 696.4 0 151 90 399 696.4 0 399 91 96 696.4 0 96 92 87 696.4 0 87 93 125 696.4 0 125 94 87 696.4 0 87 95 80 696.4 0 80 96 131 696.4 0 131 97 119 696.4 0 119 98 175 696.4 0 175 99 174 696.4 0 174 100 230 696.4 0 230 101 461 696.4 0 461 102 708 696.4 0 708 103 9013 696.4 0 9013 104 9096 696.4 0 9096 105 8000 696.4 0 8000 106 779 696.4 0 779 107 998 696.4 0 998 108 339 696.4 0 339 109 59 696.4 0 59 110 87 696.4 0 87 111 89 696.4 0 89 112 103 696.4 0 103 113 60 696.4 0 60 114 45 696.4 0 45 115 59 696.4 0 59 116 42 696.4 0 42 117 39 696.4 0 39 118 58 696.4 0 58 119 87 696.4 0 87 120 52 696.4 0 52 121 40 696.4 0 40 122 39 696.4 0 39 123 46 696.4 0 46 124 55 696.4 0 55 125 81 696.4 0 81 126 114 696.4 0 114 127 47 696.4 0 47 128 54 696.4 0 54 129 69 696.4 0 69 130 48 696.4 0 48 131 72 696.4 0 72 132 78 696.4 0 78 133 101 696.4 0 101 134 137 696.4 0 137 135 111 696.4 0 111 136 57 696.4 0 57 137 74 696.4 0 74 138 170 696.4 0 170 139 270 696.4 0 270 140 188 696.4 0 188 141 113 696.4 0 113 142 68 696.4 0 68 143 151 696.4 0 151 144 124 696.4 0 124 145 167 696.4 0 167 146 66 696.4 0 66 147 71 696.4 0 71 148 74 696.4 0 74 149 108 696.4 0 108 150 227 696.4 0 227 151 253 696.4 0 253 152 203 696.4 0 203 153 91 696.4 0 91 154 85 696.4 0 85 155 90 696.4 0 90 156 101 696.4 0 101 157 112 696.4 0 112 158 101 696.4 0 101 159 101 696.4 0 101 160 142 696.4 0 142 161 77 696.4 0 77 162 59 696.4 0 59 163 52 696.4 0 52 164 81 696.4 0 81 165 62 696.4 0 62 166 71 696.4 0 71 167 58 696.4 0 58 168 56 696.4 0 56 169 52 696.4 0 52 170 55 696.4 0 55 171 53 696.4 0 53 172 92 696.4 0 92 173 100 696.4 0 100 174 56 696.4 0 56 175 40 696.4 0 40 176 47 696.4 0 47 177 45 696.4 0 45 178 244 696.4 0 244 179 192 696.4 0 192 180 278 696.4 0 278 181 130 696.4 0 130 182 63 696.4 0 63 183 79 696.4 0 79 184 95 696.4 0 95 185 173 696.4 0 173 186 107 696.4 0 107 187 70 696.4 0 70 188 61 696.4 0 61 189 92 696.4 0 92 190 94 696.4 0 94 191 84 696.4 0 84 192 58 696.4 0 58 193 52 696.4 0 52 194 47 696.4 0 47 195 59 696.4 0 59 196 62 696.4 0 62 197 68 696.4 0 68 198 60 696.4 0 60 199 72 696.4 0 72 200 69 696.4 0 69 201 82 696.4 0 82 202 80 696.4 0 80 203 54 696.4 0 54 204 57 696.4 0 57 205 180 696.4 0 180 206 215 696.4 0 215 207 254 696.4 0 254 208 136 696.4 0 136 209 82 696.4 0 82 210 77 696.4 0 77 211 135 696.4 0 135 212 139 696.4 0 139 213 196 696.4 0 196 214 161 696.4 0 161 215 177 696.4 0 177 216 139 696.4 0 139 217 73 696.4 0 73 218 75 696.4 0 75 219 122 696.4 0 122 220 109 696.4 0 109 221 119 696.4 0 119 222 96 696.4 0 96 223 109 696.4 0 109 224 87 696.4 0 87 225 118 696.4 0 118 226 104 696.4 0 104 227 76 696.4 0 76 228 59 696.4 0 59 229 77 696.4 0 77 230 64 696.4 0 64 231 93 696.4 0 93 232 77 696.4 0 77 233 147 696.4 0 147 234 118 696.4 0 118 235 168 696.4 0 168 236 77 696.4 0 77 237 69 696.4 0 69 238 72 696.4 0 72 239 88 696.4 0 88 240 103 696.4 0 103 241 133 696.4 0 133 242 96 696.4 0 96 243 80 696.4 0 80 244 66 696.4 0 66 245 89 696.4 0 89 246 69 696.4 0 69 247 99 696.4 0 99 248 87 696.4 0 87 249 73 696.4 0 73 250 57 696.4 0 57 251 89 696.4 0 89 252 104 696.4 0 104 253 70 696.4 0 70 254 92 696.4 0 92 255 114 696.4 0 114 256 112 696.4 0 112 257 125 696.4 0 125 258 154 696.4 0 154 259 446 696.4 0 446 260 469 696.4 0 469 261 1514 696.4 0 1514 262 819 696.4 0 819 263 940 696.4 0 940 264 105 696.4 0 105 265 108 696.4 0 108 266 83 696.4 0 83 267 134 696.4 0 134 268 71 696.4 0 71 269 52 696.4 0 52 270 55 696.4 0 55 271 73 696.4 0 73 272 83 696.4 0 83 273 106 696.4 0 106 274 180 696.4 0 180 275 167 696.4 0 167 276 117 696.4 0 117 277 86 696.4 0 86 278 103 696.4 0 103 279 267 696.4 0 267 280 244 696.4 0 244 281 206 696.4 0 206 282 125 696.4 0 125 283 148 696.4 0 148 284 143 696.4 0 143 285 63 696.4 0 63 286 77 696.4 0 77 287 83 696.4 0 83 288 136 696.4 0 136 289 80 696.4 0 80 290 83 696.4 0 83 291 49 696.4 0 49 292 66 696.4 0 66 293 102 696.4 0 102 294 110 696.4 0 110 295 131 696.4 0 131 296 92 696.4 0 92 297 114 696.4 0 114 298 82 696.4 0 82 299 144 696.4 0 144 300 295 696.4 0 295 301 315 696.4 0 315 Finished in 4.41 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 97,470 Reads with adapters: 26,069 (26.7%) Reads that were too short: 70 (0.1%) Reads written (passing filters): 25,999 (26.7%) Total basepairs processed: 23,895,118 bp Total written (filtered): 7,161,594 bp (30.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26069 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 29 0.0 3 0 0 19 10 16 436 0.0 3 0 369 49 18 17 25192 0.0 3 23530 1391 221 50 18 84 0.0 3 2 56 11 15 19 114 0.0 3 8 2 62 42 20 32 0.0 3 0 0 0 32 21 3 0.0 3 1 0 1 1 22 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 1 31 2 0.0 3 0 0 0 2 33 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 66 3 0.0 3 0 0 1 2 67 5 0.0 3 0 0 1 4 68 6 0.0 3 0 0 0 6 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 1 134 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 49 0.0 3 0 0 0 49 162 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 1 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 185 3 0.0 3 0 0 0 3 186 3 0.0 3 0 0 0 3 187 7 0.0 3 0 0 0 7 188 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 242 1 0.0 3 0 1 258 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 5 0.0 3 0 0 0 5 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 25,999 Reads with adapters: 424 (1.6%) Reads written (passing filters): 25,999 (100.0%) Total basepairs processed: 7,161,594 bp Total written (filtered): 7,160,282 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 424 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.0% C: 18.2% G: 10.4% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 391 406.2 0 391 4 26 101.6 0 26 5 7 25.4 0 7