This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.52 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 106,746 (3.7%) Reads that were too short: 9,787 (0.3%) Reads written (passing filters): 96,959 (3.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,898,317 bp (2.8%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 106746 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 272 696.4 0 272 7 638 696.4 0 638 8 23593 696.4 0 23593 9 27099 696.4 0 27099 10 64 696.4 0 64 11 27 696.4 0 27 12 25 696.4 0 25 13 56 696.4 0 56 14 34 696.4 0 34 15 26 696.4 0 26 16 23 696.4 0 23 17 29 696.4 0 29 18 26 696.4 0 26 19 22 696.4 0 22 20 16 696.4 0 16 21 28 696.4 0 28 22 19 696.4 0 19 23 20 696.4 0 20 24 20 696.4 0 20 25 18 696.4 0 18 26 37 696.4 0 37 27 15 696.4 0 15 28 19 696.4 0 19 29 31 696.4 0 31 30 16 696.4 0 16 31 24 696.4 0 24 32 50 696.4 0 50 33 22 696.4 0 22 34 20 696.4 0 20 35 38 696.4 0 38 36 13 696.4 0 13 37 20 696.4 0 20 38 37 696.4 0 37 39 37 696.4 0 37 40 30 696.4 0 30 41 34 696.4 0 34 42 34 696.4 0 34 43 34 696.4 0 34 44 27 696.4 0 27 45 21 696.4 0 21 46 34 696.4 0 34 47 30 696.4 0 30 48 48 696.4 0 48 49 35 696.4 0 35 50 46 696.4 0 46 51 132 696.4 0 132 52 80 696.4 0 80 53 156 696.4 0 156 54 39 696.4 0 39 55 31 696.4 0 31 56 50 696.4 0 50 57 100 696.4 0 100 58 32 696.4 0 32 59 29 696.4 0 29 60 30 696.4 0 30 61 71 696.4 0 71 62 54 696.4 0 54 63 22 696.4 0 22 64 21 696.4 0 21 65 28 696.4 0 28 66 85 696.4 0 85 67 31 696.4 0 31 68 34 696.4 0 34 69 36 696.4 0 36 70 70 696.4 0 70 71 35 696.4 0 35 72 43 696.4 0 43 73 42 696.4 0 42 74 40 696.4 0 40 75 50 696.4 0 50 76 91 696.4 0 91 77 54 696.4 0 54 78 32 696.4 0 32 79 24 696.4 0 24 80 23 696.4 0 23 81 42 696.4 0 42 82 106 696.4 0 106 83 109 696.4 0 109 84 68 696.4 0 68 85 118 696.4 0 118 86 826 696.4 0 826 87 494 696.4 0 494 88 476 696.4 0 476 89 119 696.4 0 119 90 330 696.4 0 330 91 92 696.4 0 92 92 95 696.4 0 95 93 112 696.4 0 112 94 99 696.4 0 99 95 78 696.4 0 78 96 83 696.4 0 83 97 94 696.4 0 94 98 181 696.4 0 181 99 169 696.4 0 169 100 217 696.4 0 217 101 449 696.4 0 449 102 583 696.4 0 583 103 9806 696.4 0 9806 104 10896 696.4 0 10896 105 9521 696.4 0 9521 106 396 696.4 0 396 107 529 696.4 0 529 108 200 696.4 0 200 109 40 696.4 0 40 110 43 696.4 0 43 111 45 696.4 0 45 112 55 696.4 0 55 113 44 696.4 0 44 114 30 696.4 0 30 115 38 696.4 0 38 116 25 696.4 0 25 117 34 696.4 0 34 118 39 696.4 0 39 119 56 696.4 0 56 120 54 696.4 0 54 121 35 696.4 0 35 122 20 696.4 0 20 123 24 696.4 0 24 124 45 696.4 0 45 125 74 696.4 0 74 126 79 696.4 0 79 127 24 696.4 0 24 128 36 696.4 0 36 129 45 696.4 0 45 130 34 696.4 0 34 131 44 696.4 0 44 132 43 696.4 0 43 133 87 696.4 0 87 134 81 696.4 0 81 135 102 696.4 0 102 136 40 696.4 0 40 137 46 696.4 0 46 138 74 696.4 0 74 139 154 696.4 0 154 140 87 696.4 0 87 141 78 696.4 0 78 142 52 696.4 0 52 143 118 696.4 0 118 144 97 696.4 0 97 145 133 696.4 0 133 146 33 696.4 0 33 147 43 696.4 0 43 148 54 696.4 0 54 149 84 696.4 0 84 150 198 696.4 0 198 151 218 696.4 0 218 152 159 696.4 0 159 153 59 696.4 0 59 154 66 696.4 0 66 155 71 696.4 0 71 156 68 696.4 0 68 157 51 696.4 0 51 158 77 696.4 0 77 159 58 696.4 0 58 160 93 696.4 0 93 161 46 696.4 0 46 162 32 696.4 0 32 163 44 696.4 0 44 164 71 696.4 0 71 165 49 696.4 0 49 166 59 696.4 0 59 167 34 696.4 0 34 168 38 696.4 0 38 169 27 696.4 0 27 170 47 696.4 0 47 171 47 696.4 0 47 172 103 696.4 0 103 173 142 696.4 0 142 174 35 696.4 0 35 175 39 696.4 0 39 176 49 696.4 0 49 177 44 696.4 0 44 178 231 696.4 0 231 179 201 696.4 0 201 180 257 696.4 0 257 181 114 696.4 0 114 182 42 696.4 0 42 183 37 696.4 0 37 184 58 696.4 0 58 185 104 696.4 0 104 186 69 696.4 0 69 187 60 696.4 0 60 188 55 696.4 0 55 189 82 696.4 0 82 190 68 696.4 0 68 191 74 696.4 0 74 192 36 696.4 0 36 193 42 696.4 0 42 194 30 696.4 0 30 195 37 696.4 0 37 196 41 696.4 0 41 197 41 696.4 0 41 198 36 696.4 0 36 199 58 696.4 0 58 200 97 696.4 0 97 201 36 696.4 0 36 202 61 696.4 0 61 203 43 696.4 0 43 204 48 696.4 0 48 205 158 696.4 0 158 206 200 696.4 0 200 207 183 696.4 0 183 208 91 696.4 0 91 209 45 696.4 0 45 210 56 696.4 0 56 211 77 696.4 0 77 212 111 696.4 0 111 213 100 696.4 0 100 214 68 696.4 0 68 215 107 696.4 0 107 216 80 696.4 0 80 217 40 696.4 0 40 218 54 696.4 0 54 219 107 696.4 0 107 220 74 696.4 0 74 221 76 696.4 0 76 222 61 696.4 0 61 223 60 696.4 0 60 224 58 696.4 0 58 225 67 696.4 0 67 226 83 696.4 0 83 227 61 696.4 0 61 228 51 696.4 0 51 229 61 696.4 0 61 230 54 696.4 0 54 231 63 696.4 0 63 232 51 696.4 0 51 233 61 696.4 0 61 234 79 696.4 0 79 235 75 696.4 0 75 236 65 696.4 0 65 237 44 696.4 0 44 238 51 696.4 0 51 239 54 696.4 0 54 240 58 696.4 0 58 241 81 696.4 0 81 242 77 696.4 0 77 243 50 696.4 0 50 244 41 696.4 0 41 245 47 696.4 0 47 246 57 696.4 0 57 247 61 696.4 0 61 248 75 696.4 0 75 249 39 696.4 0 39 250 42 696.4 0 42 251 56 696.4 0 56 252 50 696.4 0 50 253 42 696.4 0 42 254 50 696.4 0 50 255 60 696.4 0 60 256 91 696.4 0 91 257 91 696.4 0 91 258 100 696.4 0 100 259 308 696.4 0 308 260 334 696.4 0 334 261 1078 696.4 0 1078 262 650 696.4 0 650 263 747 696.4 0 747 264 48 696.4 0 48 265 58 696.4 0 58 266 36 696.4 0 36 267 62 696.4 0 62 268 54 696.4 0 54 269 58 696.4 0 58 270 46 696.4 0 46 271 38 696.4 0 38 272 36 696.4 0 36 273 53 696.4 0 53 274 114 696.4 0 114 275 91 696.4 0 91 276 58 696.4 0 58 277 40 696.4 0 40 278 60 696.4 0 60 279 165 696.4 0 165 280 140 696.4 0 140 281 134 696.4 0 134 282 71 696.4 0 71 283 52 696.4 0 52 284 83 696.4 0 83 285 54 696.4 0 54 286 56 696.4 0 56 287 47 696.4 0 47 288 85 696.4 0 85 289 42 696.4 0 42 290 44 696.4 0 44 291 45 696.4 0 45 292 42 696.4 0 42 293 57 696.4 0 57 294 74 696.4 0 74 295 82 696.4 0 82 296 71 696.4 0 71 297 53 696.4 0 53 298 55 696.4 0 55 299 50 696.4 0 50 300 128 696.4 0 128 301 142 696.4 0 142 Finished in 4.70 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 96,959 Reads with adapters: 28,908 (29.8%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 28,895 (29.8%) Total basepairs processed: 23,898,317 bp Total written (filtered): 7,765,720 bp (32.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28908 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 4 19 90 0.0 3 0 85 4 1 20 27811 0.0 4 27219 564 24 0 4 21 70 0.0 4 10 59 1 22 7 0.0 4 0 0 4 0 3 23 19 0.0 4 0 0 0 18 1 31 1 0.0 4 1 38 1 0.0 4 0 1 41 2 0.0 4 0 0 0 0 2 46 1 0.0 4 1 76 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 1 116 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 10 0.0 4 0 0 0 1 9 127 868 0.0 4 0 0 0 0 868 128 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 159 1 0.0 4 1 209 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.08 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 28,895 Reads with adapters: 5,670 (19.6%) Reads written (passing filters): 28,895 (100.0%) Total basepairs processed: 7,765,720 bp Total written (filtered): 7,711,422 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5670 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.5% G: 95.2% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 451.5 0 2 4 5 112.9 0 5 8 4 0.4 0 4 9 3676 0.1 0 104 3572 10 1818 0.0 1 68 1750 11 144 0.0 1 1 143 12 9 0.0 1 0 9 13 3 0.0 1 0 3 14 1 0.0 1 0 1 31 1 0.0 1 1 151 1 0.0 1 0 1 152 1 0.0 1 0 1 160 2 0.0 1 2 161 1 0.0 1 1 208 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.