This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.95 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 280,191 (9.8%) Reads that were too short: 45,508 (1.6%) Reads written (passing filters): 234,683 (8.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 61,089,897 bp (7.1%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 280191 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1070 696.4 0 1070 7 50311 696.4 0 50311 8 38829 696.4 0 38829 9 94 696.4 0 94 10 91 696.4 0 91 11 60 696.4 0 60 12 23 696.4 0 23 13 36 696.4 0 36 14 21 696.4 0 21 15 25 696.4 0 25 16 532 696.4 0 532 17 31 696.4 0 31 18 53 696.4 0 53 19 93 696.4 0 93 20 51016 696.4 0 51016 21 12526 696.4 0 12526 22 148 696.4 0 148 23 99 696.4 0 99 24 98 696.4 0 98 25 32 696.4 0 32 26 27 696.4 0 27 27 30 696.4 0 30 28 33 696.4 0 33 29 75 696.4 0 75 30 37 696.4 0 37 31 44 696.4 0 44 32 36 696.4 0 36 33 23206 696.4 0 23206 34 734 696.4 0 734 35 48 696.4 0 48 36 38 696.4 0 38 37 35 696.4 0 35 38 43 696.4 0 43 39 46 696.4 0 46 40 39 696.4 0 39 41 47 696.4 0 47 42 31 696.4 0 31 43 52 696.4 0 52 44 51 696.4 0 51 45 42 696.4 0 42 46 47 696.4 0 47 47 46 696.4 0 46 48 84 696.4 0 84 49 62 696.4 0 62 50 65 696.4 0 65 51 37 696.4 0 37 52 50 696.4 0 50 53 54 696.4 0 54 54 69 696.4 0 69 55 117 696.4 0 117 56 83 696.4 0 83 57 64 696.4 0 64 58 63 696.4 0 63 59 53 696.4 0 53 60 173 696.4 0 173 61 102 696.4 0 102 62 94 696.4 0 94 63 96 696.4 0 96 64 81 696.4 0 81 65 109 696.4 0 109 66 80 696.4 0 80 67 252 696.4 0 252 68 85 696.4 0 85 69 99 696.4 0 99 70 144 696.4 0 144 71 135 696.4 0 135 72 130 696.4 0 130 73 172 696.4 0 172 74 173 696.4 0 173 75 321 696.4 0 321 76 1420 696.4 0 1420 77 965 696.4 0 965 78 1302 696.4 0 1302 79 441 696.4 0 441 80 1225 696.4 0 1225 81 313 696.4 0 313 82 382 696.4 0 382 83 171 696.4 0 171 84 207 696.4 0 207 85 226 696.4 0 226 86 373 696.4 0 373 87 1881 696.4 0 1881 88 3005 696.4 0 3005 89 162 696.4 0 162 90 140 696.4 0 140 91 128 696.4 0 128 92 155 696.4 0 155 93 139 696.4 0 139 94 124 696.4 0 124 95 163 696.4 0 163 96 128 696.4 0 128 97 153 696.4 0 153 98 174 696.4 0 174 99 181 696.4 0 181 100 240 696.4 0 240 101 300 696.4 0 300 102 151 696.4 0 151 103 117 696.4 0 117 104 158 696.4 0 158 105 124 696.4 0 124 106 150 696.4 0 150 107 174 696.4 0 174 108 191 696.4 0 191 109 151 696.4 0 151 110 323 696.4 0 323 111 173 696.4 0 173 112 201 696.4 0 201 113 197 696.4 0 197 114 187 696.4 0 187 115 195 696.4 0 195 116 198 696.4 0 198 117 257 696.4 0 257 118 187 696.4 0 187 119 185 696.4 0 185 120 174 696.4 0 174 121 206 696.4 0 206 122 271 696.4 0 271 123 419 696.4 0 419 124 413 696.4 0 413 125 271 696.4 0 271 126 260 696.4 0 260 127 329 696.4 0 329 128 361 696.4 0 361 129 566 696.4 0 566 130 5186 696.4 0 5186 131 3064 696.4 0 3064 132 3978 696.4 0 3978 133 222 696.4 0 222 134 235 696.4 0 235 135 252 696.4 0 252 136 1178 696.4 0 1178 137 1983 696.4 0 1983 138 1558 696.4 0 1558 139 735 696.4 0 735 140 556 696.4 0 556 141 207 696.4 0 207 142 190 696.4 0 190 143 176 696.4 0 176 144 177 696.4 0 177 145 190 696.4 0 190 146 211 696.4 0 211 147 215 696.4 0 215 148 239 696.4 0 239 149 408 696.4 0 408 150 165 696.4 0 165 151 213 696.4 0 213 152 212 696.4 0 212 153 210 696.4 0 210 154 170 696.4 0 170 155 152 696.4 0 152 156 187 696.4 0 187 157 162 696.4 0 162 158 181 696.4 0 181 159 136 696.4 0 136 160 148 696.4 0 148 161 175 696.4 0 175 162 256 696.4 0 256 163 203 696.4 0 203 164 150 696.4 0 150 165 210 696.4 0 210 166 191 696.4 0 191 167 173 696.4 0 173 168 172 696.4 0 172 169 198 696.4 0 198 170 289 696.4 0 289 171 161 696.4 0 161 172 170 696.4 0 170 173 163 696.4 0 163 174 142 696.4 0 142 175 208 696.4 0 208 176 202 696.4 0 202 177 183 696.4 0 183 178 205 696.4 0 205 179 151 696.4 0 151 180 162 696.4 0 162 181 142 696.4 0 142 182 145 696.4 0 145 183 188 696.4 0 188 184 141 696.4 0 141 185 198 696.4 0 198 186 207 696.4 0 207 187 264 696.4 0 264 188 206 696.4 0 206 189 205 696.4 0 205 190 187 696.4 0 187 191 232 696.4 0 232 192 197 696.4 0 197 193 192 696.4 0 192 194 186 696.4 0 186 195 240 696.4 0 240 196 350 696.4 0 350 197 293 696.4 0 293 198 231 696.4 0 231 199 168 696.4 0 168 200 166 696.4 0 166 201 176 696.4 0 176 202 177 696.4 0 177 203 187 696.4 0 187 204 149 696.4 0 149 205 181 696.4 0 181 206 164 696.4 0 164 207 192 696.4 0 192 208 163 696.4 0 163 209 193 696.4 0 193 210 193 696.4 0 193 211 187 696.4 0 187 212 193 696.4 0 193 213 216 696.4 0 216 214 217 696.4 0 217 215 183 696.4 0 183 216 206 696.4 0 206 217 212 696.4 0 212 218 185 696.4 0 185 219 225 696.4 0 225 220 235 696.4 0 235 221 258 696.4 0 258 222 375 696.4 0 375 223 331 696.4 0 331 224 369 696.4 0 369 225 294 696.4 0 294 226 382 696.4 0 382 227 513 696.4 0 513 228 376 696.4 0 376 229 362 696.4 0 362 230 400 696.4 0 400 231 635 696.4 0 635 232 550 696.4 0 550 233 721 696.4 0 721 234 283 696.4 0 283 235 271 696.4 0 271 236 263 696.4 0 263 237 327 696.4 0 327 238 381 696.4 0 381 239 475 696.4 0 475 240 582 696.4 0 582 241 478 696.4 0 478 242 719 696.4 0 719 243 523 696.4 0 523 244 878 696.4 0 878 245 550 696.4 0 550 246 501 696.4 0 501 247 408 696.4 0 408 248 414 696.4 0 414 249 383 696.4 0 383 250 550 696.4 0 550 251 3574 696.4 0 3574 252 3203 696.4 0 3203 253 5553 696.4 0 5553 254 328 696.4 0 328 255 293 696.4 0 293 256 835 696.4 0 835 257 742 696.4 0 742 258 1378 696.4 0 1378 259 461 696.4 0 461 260 451 696.4 0 451 261 255 696.4 0 255 262 284 696.4 0 284 263 208 696.4 0 208 264 233 696.4 0 233 265 251 696.4 0 251 266 189 696.4 0 189 267 173 696.4 0 173 268 175 696.4 0 175 269 229 696.4 0 229 270 257 696.4 0 257 271 273 696.4 0 273 272 220 696.4 0 220 273 213 696.4 0 213 274 196 696.4 0 196 275 194 696.4 0 194 276 315 696.4 0 315 277 217 696.4 0 217 278 276 696.4 0 276 279 240 696.4 0 240 280 206 696.4 0 206 281 249 696.4 0 249 282 213 696.4 0 213 283 335 696.4 0 335 284 374 696.4 0 374 285 477 696.4 0 477 286 357 696.4 0 357 287 211 696.4 0 211 288 194 696.4 0 194 289 194 696.4 0 194 290 242 696.4 0 242 291 239 696.4 0 239 292 304 696.4 0 304 293 255 696.4 0 255 294 224 696.4 0 224 295 326 696.4 0 326 296 339 696.4 0 339 297 777 696.4 0 777 298 534 696.4 0 534 299 482 696.4 0 482 300 326 696.4 0 326 301 224 696.4 0 224 Finished in 9.78 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 234,683 Reads with adapters: 49,634 (21.1%) Reads that were too short: 308 (0.1%) Reads written (passing filters): 49,326 (21.0%) Total basepairs processed: 61,089,897 bp Total written (filtered): 13,585,211 bp (22.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49634 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 91 0.0 3 0 0 35 56 16 337 0.0 3 0 220 58 59 17 47854 0.0 3 42684 3907 965 298 18 121 0.0 3 12 82 18 9 19 244 0.0 3 4 0 119 121 20 163 0.0 3 2 0 0 161 21 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 35 1 0.0 3 1 36 14 0.0 3 0 0 1 13 39 2 0.0 3 0 0 0 2 40 2 0.0 3 0 0 0 2 43 4 0.0 3 0 0 0 4 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 4 0.0 3 0 0 0 4 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 59 5 0.0 3 0 0 0 5 60 4 0.0 3 0 0 0 4 61 223 0.0 3 0 0 3 220 62 5 0.0 3 0 0 0 5 63 3 0.0 3 0 0 0 3 64 31 0.0 3 0 0 0 31 65 1 0.0 3 0 0 0 1 69 6 0.0 3 0 0 0 6 70 2 0.0 3 0 0 0 2 71 5 0.0 3 0 0 0 5 72 7 0.0 3 0 0 0 7 73 4 0.0 3 0 0 0 4 74 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 5 0.0 3 0 0 0 5 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 8 0.0 3 0 0 0 8 102 5 0.0 3 0 0 0 5 103 3 0.0 3 0 0 1 2 105 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 110 2 0.0 3 0 0 0 2 111 11 0.0 3 0 0 0 11 112 6 0.0 3 0 0 0 6 113 4 0.0 3 0 0 0 4 114 4 0.0 3 0 0 0 4 115 7 0.0 3 0 0 1 6 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 8 0.0 3 0 0 0 8 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 128 4 0.0 3 0 0 0 4 129 4 0.0 3 0 0 1 3 130 2 0.0 3 0 0 0 2 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 1 2 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 3 0.0 3 0 0 0 3 148 2 0.0 3 0 0 0 2 149 4 0.0 3 0 0 0 4 150 10 0.0 3 0 0 0 10 151 2 0.0 3 0 0 0 2 152 10 0.0 3 0 0 1 9 153 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 0 3 160 3 0.0 3 0 0 0 3 161 4 0.0 3 0 0 0 4 162 21 0.0 3 0 0 0 21 163 5 0.0 3 0 0 0 5 164 8 0.0 3 0 0 0 8 165 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 167 5 0.0 3 0 0 0 5 168 4 0.0 3 0 0 0 4 169 4 0.0 3 0 0 0 4 170 4 0.0 3 0 0 0 4 171 3 0.0 3 0 0 0 3 172 3 0.0 3 0 0 0 3 173 5 0.0 3 0 0 1 4 174 3 0.0 3 0 0 0 3 175 4 0.0 3 0 0 0 4 176 4 0.0 3 0 0 0 4 177 5 0.0 3 0 0 0 5 178 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 6 0.0 3 0 0 0 6 185 4 0.0 3 0 0 0 4 186 4 0.0 3 0 0 1 3 187 4 0.0 3 0 0 0 4 188 4 0.0 3 0 0 1 3 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 193 3 0.0 3 0 0 0 3 194 7 0.0 3 0 0 0 7 195 1 0.0 3 0 0 0 1 196 3 0.0 3 0 0 0 3 197 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 200 3 0.0 3 0 0 0 3 201 2 0.0 3 0 0 0 2 202 2 0.0 3 0 0 0 2 203 2 0.0 3 0 0 0 2 204 4 0.0 3 0 0 0 4 205 2 0.0 3 0 0 0 2 206 8 0.0 3 0 0 1 7 207 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 209 3 0.0 3 0 0 1 2 211 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 213 5 0.0 3 0 0 0 5 214 2 0.0 3 0 0 0 2 215 3 0.0 3 0 0 0 3 216 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 1 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 225 3 0.0 3 0 0 0 3 227 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 232 3 0.0 3 0 0 1 2 233 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 235 3 0.0 3 0 0 0 3 236 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 241 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 245 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 251 2 0.0 3 0 0 0 2 252 2 0.0 3 0 0 0 2 253 1 0.0 3 0 0 0 1 254 2 0.0 3 0 0 0 2 255 2 0.0 3 0 0 1 1 259 1 0.0 3 0 0 0 1 260 3 0.0 3 0 0 0 3 261 1 0.0 3 0 0 0 1 262 4 0.0 3 0 0 0 4 263 3 0.0 3 0 0 0 3 264 1 0.0 3 0 0 0 1 265 2 0.0 3 0 0 0 2 266 4 0.0 3 0 0 0 4 267 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 3 0.0 3 0 0 0 3 272 1 0.0 3 0 0 0 1 273 3 0.0 3 0 0 0 3 274 1 0.0 3 0 0 0 1 275 2 0.0 3 0 0 0 2 276 2 0.0 3 0 0 0 2 277 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 4 0.0 3 0 0 0 4 281 6 0.0 3 0 0 0 6 282 4 0.0 3 0 0 1 3 283 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.74 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 49,326 Reads with adapters: 1,512 (3.1%) Reads written (passing filters): 49,326 (100.0%) Total basepairs processed: 13,585,211 bp Total written (filtered): 13,580,595 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1512 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.1% C: 35.3% G: 8.5% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1471 770.7 0 1471 4 29 192.7 0 29 5 5 48.2 0 5 6 3 12.0 0 3 11 4 0.0 1 0 4