This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.06 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 157,713 (5.5%) Reads that were too short: 31,228 (1.1%) Reads written (passing filters): 126,485 (4.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 33,948,398 bp (4.0%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 157713 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1010 696.4 0 1010 7 43149 696.4 0 43149 8 53186 696.4 0 53186 9 123 696.4 0 123 10 86 696.4 0 86 11 70 696.4 0 70 12 30 696.4 0 30 13 49 696.4 0 49 14 17 696.4 0 17 15 22 696.4 0 22 16 21 696.4 0 21 17 40 696.4 0 40 18 42 696.4 0 42 19 48 696.4 0 48 20 38 696.4 0 38 21 58 696.4 0 58 22 62 696.4 0 62 23 69 696.4 0 69 24 39 696.4 0 39 25 41 696.4 0 41 26 36 696.4 0 36 27 34 696.4 0 34 28 28 696.4 0 28 29 40 696.4 0 40 30 23 696.4 0 23 31 57 696.4 0 57 32 43 696.4 0 43 33 57 696.4 0 57 34 39 696.4 0 39 35 34 696.4 0 34 36 55 696.4 0 55 37 25 696.4 0 25 38 44 696.4 0 44 39 47 696.4 0 47 40 39 696.4 0 39 41 33 696.4 0 33 42 37 696.4 0 37 43 46 696.4 0 46 44 58 696.4 0 58 45 48 696.4 0 48 46 68 696.4 0 68 47 73 696.4 0 73 48 137 696.4 0 137 49 154 696.4 0 154 50 91 696.4 0 91 51 39 696.4 0 39 52 49 696.4 0 49 53 51 696.4 0 51 54 64 696.4 0 64 55 151 696.4 0 151 56 87 696.4 0 87 57 44 696.4 0 44 58 32 696.4 0 32 59 38 696.4 0 38 60 156 696.4 0 156 61 55 696.4 0 55 62 44 696.4 0 44 63 68 696.4 0 68 64 51 696.4 0 51 65 60 696.4 0 60 66 66 696.4 0 66 67 249 696.4 0 249 68 48 696.4 0 48 69 46 696.4 0 46 70 67 696.4 0 67 71 81 696.4 0 81 72 50 696.4 0 50 73 49 696.4 0 49 74 72 696.4 0 72 75 229 696.4 0 229 76 1262 696.4 0 1262 77 789 696.4 0 789 78 673 696.4 0 673 79 85 696.4 0 85 80 194 696.4 0 194 81 108 696.4 0 108 82 145 696.4 0 145 83 37 696.4 0 37 84 80 696.4 0 80 85 61 696.4 0 61 86 61 696.4 0 61 87 49 696.4 0 49 88 69 696.4 0 69 89 39 696.4 0 39 90 61 696.4 0 61 91 71 696.4 0 71 92 68 696.4 0 68 93 43 696.4 0 43 94 49 696.4 0 49 95 84 696.4 0 84 96 45 696.4 0 45 97 45 696.4 0 45 98 79 696.4 0 79 99 75 696.4 0 75 100 80 696.4 0 80 101 73 696.4 0 73 102 80 696.4 0 80 103 50 696.4 0 50 104 74 696.4 0 74 105 63 696.4 0 63 106 70 696.4 0 70 107 79 696.4 0 79 108 71 696.4 0 71 109 56 696.4 0 56 110 217 696.4 0 217 111 65 696.4 0 65 112 107 696.4 0 107 113 85 696.4 0 85 114 75 696.4 0 75 115 67 696.4 0 67 116 92 696.4 0 92 117 102 696.4 0 102 118 78 696.4 0 78 119 74 696.4 0 74 120 107 696.4 0 107 121 106 696.4 0 106 122 123 696.4 0 123 123 121 696.4 0 121 124 89 696.4 0 89 125 89 696.4 0 89 126 99 696.4 0 99 127 122 696.4 0 122 128 121 696.4 0 121 129 251 696.4 0 251 130 3878 696.4 0 3878 131 2201 696.4 0 2201 132 2850 696.4 0 2850 133 101 696.4 0 101 134 101 696.4 0 101 135 114 696.4 0 114 136 1029 696.4 0 1029 137 1921 696.4 0 1921 138 1359 696.4 0 1359 139 242 696.4 0 242 140 50 696.4 0 50 141 64 696.4 0 64 142 70 696.4 0 70 143 57 696.4 0 57 144 47 696.4 0 47 145 40 696.4 0 40 146 54 696.4 0 54 147 51 696.4 0 51 148 103 696.4 0 103 149 350 696.4 0 350 150 51 696.4 0 51 151 46 696.4 0 46 152 43 696.4 0 43 153 47 696.4 0 47 154 42 696.4 0 42 155 48 696.4 0 48 156 68 696.4 0 68 157 49 696.4 0 49 158 48 696.4 0 48 159 46 696.4 0 46 160 50 696.4 0 50 161 88 696.4 0 88 162 81 696.4 0 81 163 72 696.4 0 72 164 59 696.4 0 59 165 95 696.4 0 95 166 88 696.4 0 88 167 66 696.4 0 66 168 54 696.4 0 54 169 80 696.4 0 80 170 185 696.4 0 185 171 53 696.4 0 53 172 85 696.4 0 85 173 71 696.4 0 71 174 44 696.4 0 44 175 70 696.4 0 70 176 96 696.4 0 96 177 66 696.4 0 66 178 41 696.4 0 41 179 33 696.4 0 33 180 30 696.4 0 30 181 39 696.4 0 39 182 56 696.4 0 56 183 50 696.4 0 50 184 46 696.4 0 46 185 53 696.4 0 53 186 67 696.4 0 67 187 101 696.4 0 101 188 77 696.4 0 77 189 111 696.4 0 111 190 58 696.4 0 58 191 45 696.4 0 45 192 38 696.4 0 38 193 30 696.4 0 30 194 38 696.4 0 38 195 48 696.4 0 48 196 72 696.4 0 72 197 61 696.4 0 61 198 50 696.4 0 50 199 24 696.4 0 24 200 40 696.4 0 40 201 54 696.4 0 54 202 64 696.4 0 64 203 50 696.4 0 50 204 37 696.4 0 37 205 47 696.4 0 47 206 36 696.4 0 36 207 45 696.4 0 45 208 39 696.4 0 39 209 51 696.4 0 51 210 42 696.4 0 42 211 40 696.4 0 40 212 48 696.4 0 48 213 53 696.4 0 53 214 63 696.4 0 63 215 43 696.4 0 43 216 42 696.4 0 42 217 60 696.4 0 60 218 58 696.4 0 58 219 60 696.4 0 60 220 67 696.4 0 67 221 72 696.4 0 72 222 200 696.4 0 200 223 195 696.4 0 195 224 209 696.4 0 209 225 117 696.4 0 117 226 224 696.4 0 224 227 383 696.4 0 383 228 196 696.4 0 196 229 262 696.4 0 262 230 257 696.4 0 257 231 327 696.4 0 327 232 261 696.4 0 261 233 257 696.4 0 257 234 110 696.4 0 110 235 92 696.4 0 92 236 119 696.4 0 119 237 171 696.4 0 171 238 291 696.4 0 291 239 411 696.4 0 411 240 595 696.4 0 595 241 328 696.4 0 328 242 312 696.4 0 312 243 190 696.4 0 190 244 333 696.4 0 333 245 286 696.4 0 286 246 320 696.4 0 320 247 342 696.4 0 342 248 378 696.4 0 378 249 230 696.4 0 230 250 457 696.4 0 457 251 4295 696.4 0 4295 252 3966 696.4 0 3966 253 6878 696.4 0 6878 254 164 696.4 0 164 255 84 696.4 0 84 256 197 696.4 0 197 257 191 696.4 0 191 258 326 696.4 0 326 259 106 696.4 0 106 260 120 696.4 0 120 261 85 696.4 0 85 262 131 696.4 0 131 263 96 696.4 0 96 264 70 696.4 0 70 265 88 696.4 0 88 266 82 696.4 0 82 267 87 696.4 0 87 268 55 696.4 0 55 269 86 696.4 0 86 270 108 696.4 0 108 271 73 696.4 0 73 272 84 696.4 0 84 273 74 696.4 0 74 274 51 696.4 0 51 275 52 696.4 0 52 276 84 696.4 0 84 277 63 696.4 0 63 278 73 696.4 0 73 279 81 696.4 0 81 280 62 696.4 0 62 281 66 696.4 0 66 282 75 696.4 0 75 283 209 696.4 0 209 284 250 696.4 0 250 285 397 696.4 0 397 286 244 696.4 0 244 287 93 696.4 0 93 288 88 696.4 0 88 289 95 696.4 0 95 290 112 696.4 0 112 291 173 696.4 0 173 292 176 696.4 0 176 293 146 696.4 0 146 294 149 696.4 0 149 295 281 696.4 0 281 296 277 696.4 0 277 297 700 696.4 0 700 298 321 696.4 0 321 299 307 696.4 0 307 300 167 696.4 0 167 301 120 696.4 0 120 Finished in 6.37 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 126,485 Reads with adapters: 56,286 (44.5%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 56,241 (44.5%) Total basepairs processed: 33,948,398 bp Total written (filtered): 15,309,084 bp (45.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 56286 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 6 0.0 3 0 0 6 19 224 0.0 3 0 212 9 1 2 20 55352 0.0 4 54416 900 30 2 4 21 142 0.0 4 24 114 4 22 16 0.0 4 1 0 14 1 23 58 0.0 4 2 0 0 48 8 26 1 0.0 4 1 27 2 0.0 4 2 32 1 0.0 4 1 40 1 0.0 4 1 54 12 0.0 4 0 0 0 0 12 58 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 1 74 2 0.0 4 0 0 0 0 2 99 5 0.0 4 0 0 0 1 4 101 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 121 10 0.0 4 0 0 0 1 9 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 414 0.0 4 0 0 0 0 414 129 1 0.0 4 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 1 152 1 0.0 4 0 0 1 153 1 0.0 4 1 154 5 0.0 4 1 2 0 1 1 155 7 0.0 4 5 2 156 1 0.0 4 0 0 1 157 1 0.0 4 0 0 1 159 4 0.0 4 0 0 0 0 4 172 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.97 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 56,241 Reads with adapters: 5,048 (9.0%) Reads written (passing filters): 56,241 (100.0%) Total basepairs processed: 15,309,084 bp Total written (filtered): 15,253,422 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5048 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 3.1% G: 93.9% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 28 878.8 0 28 4 6 219.7 0 6 5 2 54.9 0 2 6 2 13.7 0 2 7 2 3.4 0 2 8 2 0.9 0 2 9 120 0.2 0 5 115 10 2018 0.1 1 71 1947 11 2842 0.0 1 21 2821 12 10 0.0 1 1 9 13 1 0.0 1 0 1 15 1 0.0 1 0 1 151 1 0.0 1 0 1 190 5 0.0 1 4 1 209 7 0.0 1 3 4 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.