This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.85 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 348,217 (12.2%) Reads that were too short: 297,329 (10.4%) Reads written (passing filters): 50,888 (1.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 10,486,896 bp (1.2%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 348217 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 807 696.4 0 807 7 9419 696.4 0 9419 8 560 696.4 0 560 9 10774 696.4 0 10774 10 49 696.4 0 49 11 29 696.4 0 29 12 16 696.4 0 16 13 39 696.4 0 39 14 16 696.4 0 16 15 16 696.4 0 16 16 20 696.4 0 20 17 19 696.4 0 19 18 42 696.4 0 42 19 59 696.4 0 59 20 43 696.4 0 43 21 34 696.4 0 34 22 28 696.4 0 28 23 23 696.4 0 23 24 10 696.4 0 10 25 17 696.4 0 17 26 10 696.4 0 10 27 9 696.4 0 9 28 22 696.4 0 22 29 17 696.4 0 17 30 19 696.4 0 19 31 10 696.4 0 10 32 12 696.4 0 12 33 18 696.4 0 18 34 17 696.4 0 17 35 11 696.4 0 11 36 21 696.4 0 21 37 10 696.4 0 10 38 19 696.4 0 19 39 29 696.4 0 29 40 27 696.4 0 27 41 19 696.4 0 19 42 31 696.4 0 31 43 36 696.4 0 36 44 21 696.4 0 21 45 45 696.4 0 45 46 51 696.4 0 51 47 159 696.4 0 159 48 65 696.4 0 65 49 51 696.4 0 51 50 31 696.4 0 31 51 33 696.4 0 33 52 21 696.4 0 21 53 47 696.4 0 47 54 79 696.4 0 79 55 68 696.4 0 68 56 45 696.4 0 45 57 29 696.4 0 29 58 27 696.4 0 27 59 26 696.4 0 26 60 27 696.4 0 27 61 27 696.4 0 27 62 58 696.4 0 58 63 38 696.4 0 38 64 38 696.4 0 38 65 30 696.4 0 30 66 22 696.4 0 22 67 25 696.4 0 25 68 17 696.4 0 17 69 74 696.4 0 74 70 44 696.4 0 44 71 98 696.4 0 98 72 192 696.4 0 192 73 100 696.4 0 100 74 127 696.4 0 127 75 14 696.4 0 14 76 17 696.4 0 17 77 13 696.4 0 13 78 22 696.4 0 22 79 17 696.4 0 17 80 23 696.4 0 23 81 25 696.4 0 25 82 19 696.4 0 19 83 51 696.4 0 51 84 44 696.4 0 44 85 55 696.4 0 55 86 38 696.4 0 38 87 52 696.4 0 52 88 34 696.4 0 34 89 40 696.4 0 40 90 29 696.4 0 29 91 24 696.4 0 24 92 25 696.4 0 25 93 34 696.4 0 34 94 19 696.4 0 19 95 20 696.4 0 20 96 17 696.4 0 17 97 20 696.4 0 20 98 12 696.4 0 12 99 25 696.4 0 25 100 22 696.4 0 22 101 13 696.4 0 13 102 10 696.4 0 10 103 14 696.4 0 14 104 23 696.4 0 23 105 31 696.4 0 31 106 20 696.4 0 20 107 23 696.4 0 23 108 38 696.4 0 38 109 23 696.4 0 23 110 24 696.4 0 24 111 18 696.4 0 18 112 50 696.4 0 50 113 70 696.4 0 70 114 66 696.4 0 66 115 46 696.4 0 46 116 116 696.4 0 116 117 81 696.4 0 81 118 83 696.4 0 83 119 35 696.4 0 35 120 33 696.4 0 33 121 40 696.4 0 40 122 71 696.4 0 71 123 352 696.4 0 352 124 383 696.4 0 383 125 775 696.4 0 775 126 149 696.4 0 149 127 58 696.4 0 58 128 175 696.4 0 175 129 43 696.4 0 43 130 55 696.4 0 55 131 54 696.4 0 54 132 30 696.4 0 30 133 20 696.4 0 20 134 39 696.4 0 39 135 34 696.4 0 34 136 35 696.4 0 35 137 45 696.4 0 45 138 63 696.4 0 63 139 62 696.4 0 62 140 28 696.4 0 28 141 30 696.4 0 30 142 32 696.4 0 32 143 38 696.4 0 38 144 33 696.4 0 33 145 44 696.4 0 44 146 52 696.4 0 52 147 64 696.4 0 64 148 49 696.4 0 49 149 70 696.4 0 70 150 400 696.4 0 400 151 282 696.4 0 282 152 561 696.4 0 561 153 116 696.4 0 116 154 77 696.4 0 77 155 90 696.4 0 90 156 87 696.4 0 87 157 83 696.4 0 83 158 55 696.4 0 55 159 103 696.4 0 103 160 388 696.4 0 388 161 217 696.4 0 217 162 322 696.4 0 322 163 128 696.4 0 128 164 240 696.4 0 240 165 1325 696.4 0 1325 166 974 696.4 0 974 167 1188 696.4 0 1188 168 556 696.4 0 556 169 164 696.4 0 164 170 179 696.4 0 179 171 115 696.4 0 115 172 144 696.4 0 144 173 131 696.4 0 131 174 77 696.4 0 77 175 134 696.4 0 134 176 129 696.4 0 129 177 196 696.4 0 196 178 150 696.4 0 150 179 162 696.4 0 162 180 161 696.4 0 161 181 128 696.4 0 128 182 148 696.4 0 148 183 191 696.4 0 191 184 258 696.4 0 258 185 1184 696.4 0 1184 186 1168 696.4 0 1168 187 4931 696.4 0 4931 188 1687 696.4 0 1687 189 1369 696.4 0 1369 190 96 696.4 0 96 191 75 696.4 0 75 192 104 696.4 0 104 193 112 696.4 0 112 194 139 696.4 0 139 195 186 696.4 0 186 196 291 696.4 0 291 197 274 696.4 0 274 198 200 696.4 0 200 199 257 696.4 0 257 200 501 696.4 0 501 201 132 696.4 0 132 202 90 696.4 0 90 203 102 696.4 0 102 204 98 696.4 0 98 205 125 696.4 0 125 206 70 696.4 0 70 207 108 696.4 0 108 208 71 696.4 0 71 209 90 696.4 0 90 210 329 696.4 0 329 211 103 696.4 0 103 212 92 696.4 0 92 213 91 696.4 0 91 214 128 696.4 0 128 215 126 696.4 0 126 216 255 696.4 0 255 217 453 696.4 0 453 218 756 696.4 0 756 219 917 696.4 0 917 220 1456 696.4 0 1456 221 5928 696.4 0 5928 222 15811 696.4 0 15811 223 10973 696.4 0 10973 224 5632 696.4 0 5632 225 843 696.4 0 843 226 708 696.4 0 708 227 1110 696.4 0 1110 228 424 696.4 0 424 229 356 696.4 0 356 230 344 696.4 0 344 231 421 696.4 0 421 232 810 696.4 0 810 233 922 696.4 0 922 234 1526 696.4 0 1526 235 3170 696.4 0 3170 236 6511 696.4 0 6511 237 75645 696.4 0 75645 238 67490 696.4 0 67490 239 51761 696.4 0 51761 240 4223 696.4 0 4223 241 758 696.4 0 758 242 208 696.4 0 208 243 158 696.4 0 158 244 351 696.4 0 351 245 3348 696.4 0 3348 246 2551 696.4 0 2551 247 6622 696.4 0 6622 248 2608 696.4 0 2608 249 1759 696.4 0 1759 250 742 696.4 0 742 251 266 696.4 0 266 252 102 696.4 0 102 253 39 696.4 0 39 254 43 696.4 0 43 255 101 696.4 0 101 256 113 696.4 0 113 257 92 696.4 0 92 258 60 696.4 0 60 259 43 696.4 0 43 260 118 696.4 0 118 261 99 696.4 0 99 262 131 696.4 0 131 263 97 696.4 0 97 264 129 696.4 0 129 265 116 696.4 0 116 266 182 696.4 0 182 267 404 696.4 0 404 268 918 696.4 0 918 269 1150 696.4 0 1150 270 856 696.4 0 856 271 385 696.4 0 385 272 203 696.4 0 203 273 236 696.4 0 236 274 214 696.4 0 214 275 177 696.4 0 177 276 163 696.4 0 163 277 178 696.4 0 178 278 165 696.4 0 165 279 173 696.4 0 173 280 301 696.4 0 301 281 560 696.4 0 560 282 545 696.4 0 545 283 726 696.4 0 726 284 156 696.4 0 156 285 183 696.4 0 183 286 264 696.4 0 264 287 579 696.4 0 579 288 610 696.4 0 610 289 1146 696.4 0 1146 290 906 696.4 0 906 291 713 696.4 0 713 292 283 696.4 0 283 293 244 696.4 0 244 294 931 696.4 0 931 295 806 696.4 0 806 296 1171 696.4 0 1171 297 435 696.4 0 435 298 193 696.4 0 193 299 168 696.4 0 168 300 113 696.4 0 113 301 170 696.4 0 170 Finished in 2.07 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 50,888 Reads with adapters: 11,941 (23.5%) Reads that were too short: 206 (0.4%) Reads written (passing filters): 11,735 (23.1%) Total basepairs processed: 10,486,896 bp Total written (filtered): 3,192,970 bp (30.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11941 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 3 6 16 57 0.0 3 0 42 8 7 17 11344 0.0 3 10603 598 117 26 18 41 0.0 3 9 26 3 3 19 24 0.0 3 0 0 14 10 20 19 0.0 3 1 0 0 18 21 67 0.0 3 0 0 3 64 22 2 0.0 3 0 0 0 2 24 7 0.0 3 0 0 0 7 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 5 0.0 3 0 0 0 5 30 6 0.0 3 0 0 0 6 31 1 0.0 3 0 0 0 1 32 44 0.0 3 0 0 0 44 33 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 6 0.0 3 0 0 0 6 48 41 0.0 3 0 0 0 41 50 6 0.0 3 0 0 1 5 51 9 0.0 3 0 0 0 9 53 2 0.0 3 1 0 0 1 57 17 0.0 3 0 0 0 17 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 60 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 6 0.0 3 0 0 0 6 67 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 72 8 0.0 3 0 0 0 8 74 6 0.0 3 0 0 0 6 81 2 0.0 3 0 0 0 2 83 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 5 0.0 3 0 0 0 5 102 5 0.0 3 0 0 2 3 105 3 0.0 3 0 0 0 3 106 3 0.0 3 0 0 0 3 107 6 0.0 3 0 0 0 6 108 9 0.0 3 0 0 0 9 109 4 0.0 3 0 0 0 4 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 18 0.0 3 0 0 0 18 119 4 0.0 3 0 0 0 4 120 1 0.0 3 0 0 0 1 121 11 0.0 3 0 0 0 11 122 7 0.0 3 0 0 1 6 123 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 4 0.0 3 0 0 0 4 127 3 0.0 3 0 0 0 3 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 135 4 0.0 3 0 0 0 4 136 5 0.0 3 0 0 0 5 137 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 1 152 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 3 0.0 3 0 0 1 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 1 219 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 1 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 11,735 Reads with adapters: 465 (4.0%) Reads written (passing filters): 11,735 (100.0%) Total basepairs processed: 3,192,970 bp Total written (filtered): 3,191,567 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 465 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.6% C: 15.1% G: 22.4% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 457 183.4 0 457 4 8 45.8 0 8