This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.97 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 387,050 (13.6%) Reads that were too short: 345,317 (12.1%) Reads written (passing filters): 41,733 (1.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 9,172,943 bp (1.1%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 387050 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1006 696.4 0 1006 7 11392 696.4 0 11392 8 273 696.4 0 273 9 9409 696.4 0 9409 10 44 696.4 0 44 11 22 696.4 0 22 12 18 696.4 0 18 13 24 696.4 0 24 14 14 696.4 0 14 15 14 696.4 0 14 16 15 696.4 0 15 17 15 696.4 0 15 18 19 696.4 0 19 19 21 696.4 0 21 20 24 696.4 0 24 21 26 696.4 0 26 22 20 696.4 0 20 23 24 696.4 0 24 24 11 696.4 0 11 25 9 696.4 0 9 26 11 696.4 0 11 27 15 696.4 0 15 28 17 696.4 0 17 29 8 696.4 0 8 30 15 696.4 0 15 31 16 696.4 0 16 32 10 696.4 0 10 33 14 696.4 0 14 34 15 696.4 0 15 35 19 696.4 0 19 36 16 696.4 0 16 37 12 696.4 0 12 38 15 696.4 0 15 39 26 696.4 0 26 40 21 696.4 0 21 41 19 696.4 0 19 42 28 696.4 0 28 43 19 696.4 0 19 44 12 696.4 0 12 45 25 696.4 0 25 46 14 696.4 0 14 47 28 696.4 0 28 48 20 696.4 0 20 49 38 696.4 0 38 50 23 696.4 0 23 51 27 696.4 0 27 52 30 696.4 0 30 53 57 696.4 0 57 54 51 696.4 0 51 55 58 696.4 0 58 56 41 696.4 0 41 57 20 696.4 0 20 58 22 696.4 0 22 59 23 696.4 0 23 60 23 696.4 0 23 61 27 696.4 0 27 62 26 696.4 0 26 63 31 696.4 0 31 64 24 696.4 0 24 65 19 696.4 0 19 66 34 696.4 0 34 67 26 696.4 0 26 68 22 696.4 0 22 69 16 696.4 0 16 70 25 696.4 0 25 71 23 696.4 0 23 72 44 696.4 0 44 73 34 696.4 0 34 74 51 696.4 0 51 75 19 696.4 0 19 76 21 696.4 0 21 77 18 696.4 0 18 78 13 696.4 0 13 79 21 696.4 0 21 80 20 696.4 0 20 81 24 696.4 0 24 82 24 696.4 0 24 83 27 696.4 0 27 84 34 696.4 0 34 85 33 696.4 0 33 86 28 696.4 0 28 87 38 696.4 0 38 88 20 696.4 0 20 89 14 696.4 0 14 90 18 696.4 0 18 91 26 696.4 0 26 92 26 696.4 0 26 93 25 696.4 0 25 94 17 696.4 0 17 95 31 696.4 0 31 96 21 696.4 0 21 97 19 696.4 0 19 98 21 696.4 0 21 99 28 696.4 0 28 100 22 696.4 0 22 101 24 696.4 0 24 102 20 696.4 0 20 103 24 696.4 0 24 104 24 696.4 0 24 105 21 696.4 0 21 106 18 696.4 0 18 107 24 696.4 0 24 108 25 696.4 0 25 109 22 696.4 0 22 110 20 696.4 0 20 111 15 696.4 0 15 112 30 696.4 0 30 113 39 696.4 0 39 114 31 696.4 0 31 115 43 696.4 0 43 116 80 696.4 0 80 117 36 696.4 0 36 118 34 696.4 0 34 119 35 696.4 0 35 120 35 696.4 0 35 121 33 696.4 0 33 122 54 696.4 0 54 123 86 696.4 0 86 124 92 696.4 0 92 125 251 696.4 0 251 126 121 696.4 0 121 127 53 696.4 0 53 128 187 696.4 0 187 129 45 696.4 0 45 130 32 696.4 0 32 131 34 696.4 0 34 132 25 696.4 0 25 133 15 696.4 0 15 134 34 696.4 0 34 135 24 696.4 0 24 136 28 696.4 0 28 137 30 696.4 0 30 138 57 696.4 0 57 139 30 696.4 0 30 140 29 696.4 0 29 141 28 696.4 0 28 142 24 696.4 0 24 143 32 696.4 0 32 144 36 696.4 0 36 145 36 696.4 0 36 146 26 696.4 0 26 147 37 696.4 0 37 148 24 696.4 0 24 149 48 696.4 0 48 150 135 696.4 0 135 151 111 696.4 0 111 152 163 696.4 0 163 153 43 696.4 0 43 154 46 696.4 0 46 155 35 696.4 0 35 156 39 696.4 0 39 157 27 696.4 0 27 158 36 696.4 0 36 159 47 696.4 0 47 160 118 696.4 0 118 161 70 696.4 0 70 162 118 696.4 0 118 163 38 696.4 0 38 164 85 696.4 0 85 165 432 696.4 0 432 166 258 696.4 0 258 167 372 696.4 0 372 168 159 696.4 0 159 169 64 696.4 0 64 170 59 696.4 0 59 171 48 696.4 0 48 172 53 696.4 0 53 173 59 696.4 0 59 174 36 696.4 0 36 175 39 696.4 0 39 176 45 696.4 0 45 177 50 696.4 0 50 178 63 696.4 0 63 179 87 696.4 0 87 180 66 696.4 0 66 181 78 696.4 0 78 182 91 696.4 0 91 183 104 696.4 0 104 184 132 696.4 0 132 185 546 696.4 0 546 186 489 696.4 0 489 187 5734 696.4 0 5734 188 2045 696.4 0 2045 189 1905 696.4 0 1905 190 49 696.4 0 49 191 31 696.4 0 31 192 37 696.4 0 37 193 27 696.4 0 27 194 60 696.4 0 60 195 70 696.4 0 70 196 52 696.4 0 52 197 54 696.4 0 54 198 67 696.4 0 67 199 111 696.4 0 111 200 573 696.4 0 573 201 108 696.4 0 108 202 56 696.4 0 56 203 96 696.4 0 96 204 55 696.4 0 55 205 39 696.4 0 39 206 34 696.4 0 34 207 96 696.4 0 96 208 47 696.4 0 47 209 73 696.4 0 73 210 409 696.4 0 409 211 67 696.4 0 67 212 45 696.4 0 45 213 65 696.4 0 65 214 58 696.4 0 58 215 49 696.4 0 49 216 81 696.4 0 81 217 197 696.4 0 197 218 202 696.4 0 202 219 391 696.4 0 391 220 933 696.4 0 933 221 2312 696.4 0 2312 222 6269 696.4 0 6269 223 4865 696.4 0 4865 224 2660 696.4 0 2660 225 466 696.4 0 466 226 290 696.4 0 290 227 410 696.4 0 410 228 123 696.4 0 123 229 233 696.4 0 233 230 320 696.4 0 320 231 444 696.4 0 444 232 933 696.4 0 933 233 1152 696.4 0 1152 234 2003 696.4 0 2003 235 4060 696.4 0 4060 236 7072 696.4 0 7072 237 112280 696.4 0 112280 238 103206 696.4 0 103206 239 76394 696.4 0 76394 240 4100 696.4 0 4100 241 594 696.4 0 594 242 80 696.4 0 80 243 58 696.4 0 58 244 127 696.4 0 127 245 1156 696.4 0 1156 246 741 696.4 0 741 247 2235 696.4 0 2235 248 948 696.4 0 948 249 513 696.4 0 513 250 227 696.4 0 227 251 83 696.4 0 83 252 42 696.4 0 42 253 17 696.4 0 17 254 23 696.4 0 23 255 34 696.4 0 34 256 20 696.4 0 20 257 36 696.4 0 36 258 26 696.4 0 26 259 19 696.4 0 19 260 43 696.4 0 43 261 49 696.4 0 49 262 29 696.4 0 29 263 33 696.4 0 33 264 35 696.4 0 35 265 37 696.4 0 37 266 60 696.4 0 60 267 165 696.4 0 165 268 398 696.4 0 398 269 495 696.4 0 495 270 387 696.4 0 387 271 154 696.4 0 154 272 71 696.4 0 71 273 84 696.4 0 84 274 94 696.4 0 94 275 59 696.4 0 59 276 58 696.4 0 58 277 56 696.4 0 56 278 67 696.4 0 67 279 63 696.4 0 63 280 78 696.4 0 78 281 176 696.4 0 176 282 174 696.4 0 174 283 243 696.4 0 243 284 46 696.4 0 46 285 66 696.4 0 66 286 71 696.4 0 71 287 183 696.4 0 183 288 184 696.4 0 184 289 259 696.4 0 259 290 227 696.4 0 227 291 130 696.4 0 130 292 57 696.4 0 57 293 60 696.4 0 60 294 346 696.4 0 346 295 295 696.4 0 295 296 365 696.4 0 365 297 173 696.4 0 173 298 47 696.4 0 47 299 55 696.4 0 55 300 37 696.4 0 37 301 44 696.4 0 44 Finished in 1.91 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 41,733 Reads with adapters: 12,178 (29.2%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 12,171 (29.2%) Total basepairs processed: 9,172,943 bp Total written (filtered): 3,335,035 bp (36.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12178 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 1 0.0 3 0 0 1 19 78 0.0 3 0 74 3 0 1 20 12036 0.0 4 11810 217 8 1 21 34 0.0 4 6 28 22 7 0.0 4 1 0 2 0 4 23 8 0.0 4 0 0 0 8 62 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 254 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 12,171 Reads with adapters: 358 (2.9%) Reads written (passing filters): 12,171 (100.0%) Total basepairs processed: 3,335,035 bp Total written (filtered): 3,330,639 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 358 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 16.8% C: 3.4% G: 79.3% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 190.2 0 26 4 1 47.5 0 1 10 2 0.0 1 0 2 11 277 0.0 1 0 277 12 17 0.0 1 0 17 20 31 0.0 1 25 6 21 2 0.0 1 1 1 171 1 0.0 1 1 210 1 0.0 1 0 1