This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.12 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 424,229 (14.4%) Reads that were too short: 9,363 (0.3%) Reads written (passing filters): 414,866 (14.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 72,596,688 bp (8.2%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 424229 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 474 720.2 0 474 7 33859 720.2 0 33859 8 2471 720.2 0 2471 9 26865 720.2 0 26865 10 73 720.2 0 73 11 26 720.2 0 26 12 36 720.2 0 36 13 108 720.2 0 108 14 141 720.2 0 141 15 94 720.2 0 94 16 62 720.2 0 62 17 38 720.2 0 38 18 39 720.2 0 39 19 54 720.2 0 54 20 46 720.2 0 46 21 52 720.2 0 52 22 25 720.2 0 25 23 37 720.2 0 37 24 47 720.2 0 47 25 70 720.2 0 70 26 114 720.2 0 114 27 631 720.2 0 631 28 676 720.2 0 676 29 1301 720.2 0 1301 30 38 720.2 0 38 31 90 720.2 0 90 32 37 720.2 0 37 33 38 720.2 0 38 34 58 720.2 0 58 35 55 720.2 0 55 36 40 720.2 0 40 37 83 720.2 0 83 38 33 720.2 0 33 39 29 720.2 0 29 40 51 720.2 0 51 41 48 720.2 0 48 42 65 720.2 0 65 43 59 720.2 0 59 44 72 720.2 0 72 45 59 720.2 0 59 46 44 720.2 0 44 47 56 720.2 0 56 48 62 720.2 0 62 49 81 720.2 0 81 50 74 720.2 0 74 51 80 720.2 0 80 52 107 720.2 0 107 53 68 720.2 0 68 54 44 720.2 0 44 55 57 720.2 0 57 56 45 720.2 0 45 57 142 720.2 0 142 58 268 720.2 0 268 59 504 720.2 0 504 60 58 720.2 0 58 61 48 720.2 0 48 62 43 720.2 0 43 63 52 720.2 0 52 64 43 720.2 0 43 65 69 720.2 0 69 66 44 720.2 0 44 67 48 720.2 0 48 68 46 720.2 0 46 69 54 720.2 0 54 70 50 720.2 0 50 71 50 720.2 0 50 72 40 720.2 0 40 73 58 720.2 0 58 74 48 720.2 0 48 75 54 720.2 0 54 76 64 720.2 0 64 77 61 720.2 0 61 78 59 720.2 0 59 79 61 720.2 0 61 80 67 720.2 0 67 81 133 720.2 0 133 82 42 720.2 0 42 83 49 720.2 0 49 84 61 720.2 0 61 85 74 720.2 0 74 86 44 720.2 0 44 87 45 720.2 0 45 88 58 720.2 0 58 89 74 720.2 0 74 90 82 720.2 0 82 91 181 720.2 0 181 92 96 720.2 0 96 93 115 720.2 0 115 94 110 720.2 0 110 95 158 720.2 0 158 96 167 720.2 0 167 97 84 720.2 0 84 98 82 720.2 0 82 99 63 720.2 0 63 100 83 720.2 0 83 101 118 720.2 0 118 102 138 720.2 0 138 103 198 720.2 0 198 104 325 720.2 0 325 105 391 720.2 0 391 106 753 720.2 0 753 107 975 720.2 0 975 108 16987 720.2 0 16987 109 8887 720.2 0 8887 110 26860 720.2 0 26860 111 230 720.2 0 230 112 73 720.2 0 73 113 66 720.2 0 66 114 55 720.2 0 55 115 69 720.2 0 69 116 104 720.2 0 104 117 94 720.2 0 94 118 91 720.2 0 91 119 64 720.2 0 64 120 127 720.2 0 127 121 182 720.2 0 182 122 98 720.2 0 98 123 101 720.2 0 101 124 148 720.2 0 148 125 130 720.2 0 130 126 101 720.2 0 101 127 152 720.2 0 152 128 214 720.2 0 214 129 241 720.2 0 241 130 168 720.2 0 168 131 259 720.2 0 259 132 276 720.2 0 276 133 366 720.2 0 366 134 5790 720.2 0 5790 135 6187 720.2 0 6187 136 11783 720.2 0 11783 137 409 720.2 0 409 138 159 720.2 0 159 139 96 720.2 0 96 140 148 720.2 0 148 141 225 720.2 0 225 142 272 720.2 0 272 143 291 720.2 0 291 144 344 720.2 0 344 145 494 720.2 0 494 146 1103 720.2 0 1103 147 2218 720.2 0 2218 148 455 720.2 0 455 149 677 720.2 0 677 150 842 720.2 0 842 151 404 720.2 0 404 152 444 720.2 0 444 153 942 720.2 0 942 154 1054 720.2 0 1054 155 3239 720.2 0 3239 156 1574 720.2 0 1574 157 4416 720.2 0 4416 158 2451 720.2 0 2451 159 3597 720.2 0 3597 160 56399 720.2 0 56399 161 108688 720.2 0 108688 162 65339 720.2 0 65339 163 315 720.2 0 315 164 104 720.2 0 104 165 90 720.2 0 90 166 80 720.2 0 80 167 74 720.2 0 74 168 51 720.2 0 51 169 56 720.2 0 56 170 55 720.2 0 55 171 68 720.2 0 68 172 41 720.2 0 41 173 50 720.2 0 50 174 75 720.2 0 75 175 75 720.2 0 75 176 89 720.2 0 89 177 49 720.2 0 49 178 79 720.2 0 79 179 95 720.2 0 95 180 103 720.2 0 103 181 94 720.2 0 94 182 60 720.2 0 60 183 69 720.2 0 69 184 56 720.2 0 56 185 56 720.2 0 56 186 52 720.2 0 52 187 62 720.2 0 62 188 49 720.2 0 49 189 59 720.2 0 59 190 44 720.2 0 44 191 226 720.2 0 226 192 111 720.2 0 111 193 181 720.2 0 181 194 58 720.2 0 58 195 50 720.2 0 50 196 43 720.2 0 43 197 35 720.2 0 35 198 44 720.2 0 44 199 49 720.2 0 49 200 42 720.2 0 42 201 52 720.2 0 52 202 63 720.2 0 63 203 73 720.2 0 73 204 83 720.2 0 83 205 69 720.2 0 69 206 44 720.2 0 44 207 55 720.2 0 55 208 83 720.2 0 83 209 62 720.2 0 62 210 44 720.2 0 44 211 38 720.2 0 38 212 38 720.2 0 38 213 31 720.2 0 31 214 46 720.2 0 46 215 47 720.2 0 47 216 49 720.2 0 49 217 53 720.2 0 53 218 80 720.2 0 80 219 78 720.2 0 78 220 56 720.2 0 56 221 137 720.2 0 137 222 358 720.2 0 358 223 187 720.2 0 187 224 146 720.2 0 146 225 77 720.2 0 77 226 61 720.2 0 61 227 54 720.2 0 54 228 59 720.2 0 59 229 46 720.2 0 46 230 60 720.2 0 60 231 63 720.2 0 63 232 93 720.2 0 93 233 77 720.2 0 77 234 101 720.2 0 101 235 104 720.2 0 104 236 138 720.2 0 138 237 148 720.2 0 148 238 40 720.2 0 40 239 41 720.2 0 41 240 29 720.2 0 29 241 55 720.2 0 55 242 89 720.2 0 89 243 94 720.2 0 94 244 44 720.2 0 44 245 44 720.2 0 44 246 39 720.2 0 39 247 52 720.2 0 52 248 63 720.2 0 63 249 61 720.2 0 61 250 130 720.2 0 130 251 297 720.2 0 297 252 379 720.2 0 379 253 644 720.2 0 644 254 458 720.2 0 458 255 232 720.2 0 232 256 258 720.2 0 258 257 78 720.2 0 78 258 47 720.2 0 47 259 36 720.2 0 36 260 46 720.2 0 46 261 77 720.2 0 77 262 95 720.2 0 95 263 121 720.2 0 121 264 51 720.2 0 51 265 84 720.2 0 84 266 57 720.2 0 57 267 68 720.2 0 68 268 43 720.2 0 43 269 76 720.2 0 76 270 93 720.2 0 93 271 102 720.2 0 102 272 82 720.2 0 82 273 44 720.2 0 44 274 40 720.2 0 40 275 49 720.2 0 49 276 39 720.2 0 39 277 73 720.2 0 73 278 105 720.2 0 105 279 121 720.2 0 121 280 117 720.2 0 117 281 98 720.2 0 98 282 130 720.2 0 130 283 227 720.2 0 227 284 116 720.2 0 116 285 53 720.2 0 53 286 39 720.2 0 39 287 45 720.2 0 45 288 37 720.2 0 37 289 41 720.2 0 41 290 56 720.2 0 56 291 70 720.2 0 70 292 82 720.2 0 82 293 82 720.2 0 82 294 49 720.2 0 49 295 41 720.2 0 41 296 64 720.2 0 64 297 69 720.2 0 69 298 78 720.2 0 78 299 65 720.2 0 65 300 52 720.2 0 52 301 75 720.2 0 75 Finished in 22.51 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 414,866 Reads with adapters: 39,175 (9.4%) Reads that were too short: 424 (0.1%) Reads written (passing filters): 38,751 (9.3%) Total basepairs processed: 72,596,688 bp Total written (filtered): 9,897,209 bp (13.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 39175 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 15 0.0 3 0 0 9 6 16 300 0.0 3 0 244 32 24 17 33192 0.0 3 31500 1435 204 53 18 69 0.0 3 3 52 3 11 19 88 0.0 3 0 1 53 34 20 63 0.0 3 1 0 0 62 23 2 0.0 3 1 0 0 1 26 3 0.0 3 0 0 0 3 29 1 0.0 3 1 30 8 0.0 3 0 0 0 8 31 4542 0.0 3 0 0 18 4524 33 2 0.0 3 0 0 0 2 34 339 0.0 3 0 0 8 331 36 1 0.0 3 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 0 5 45 1 0.0 3 0 0 0 1 47 3 0.0 3 0 1 1 1 49 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 57 54 0.0 3 0 0 2 52 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 14 0.0 3 0 0 0 14 61 1 0.0 3 0 0 0 1 63 12 0.0 3 0 0 0 12 65 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 82 52 0.0 3 0 0 1 51 83 6 0.0 3 0 0 0 6 84 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 11 0.0 3 0 0 0 11 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 2 1 96 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 12 0.0 3 0 0 0 12 109 4 0.0 3 1 0 0 3 110 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 1 1 123 16 0.0 3 0 0 0 16 131 1 0.0 3 0 0 0 1 132 173 0.0 3 0 0 1 172 133 16 0.0 3 0 0 0 16 134 12 0.0 3 0 0 0 12 135 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 158 11 0.0 3 0 0 0 11 159 24 0.0 3 0 0 0 24 162 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 0 3 172 2 0.0 3 0 0 1 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 179 5 0.0 3 0 0 0 5 180 6 0.0 3 0 0 0 6 187 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 206 4 0.0 3 0 0 0 4 214 1 0.0 3 0 1 218 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 5 0.0 3 0 0 0 5 283 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.26 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 38,751 Reads with adapters: 3,502 (9.0%) Reads written (passing filters): 38,751 (100.0%) Total basepairs processed: 9,897,209 bp Total written (filtered): 9,886,674 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3502 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.7% C: 14.4% G: 5.3% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3482 605.5 0 3482 4 13 151.4 0 13 5 5 37.8 0 5 6 2 9.5 0 2