This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGGCCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 137.35 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 125,649 (4.3%) Reads that were too short: 64,815 (2.2%) Reads written (passing filters): 60,834 (2.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 12,967,478 bp (1.5%) === Adapter 1 === Sequence: CGGCCA; Type: regular 5'; Length: 6; Trimmed: 125649 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2803 720.2 0 2803 7 9274 720.2 0 9274 8 500 720.2 0 500 9 11838 720.2 0 11838 10 27 720.2 0 27 11 64 720.2 0 64 12 42 720.2 0 42 13 20 720.2 0 20 14 23 720.2 0 23 15 12 720.2 0 12 16 19 720.2 0 19 17 25 720.2 0 25 18 21 720.2 0 21 19 20 720.2 0 20 20 12 720.2 0 12 21 17 720.2 0 17 22 19 720.2 0 19 23 27 720.2 0 27 24 36 720.2 0 36 25 17 720.2 0 17 26 17 720.2 0 17 27 26 720.2 0 26 28 25 720.2 0 25 29 14 720.2 0 14 30 35 720.2 0 35 31 30 720.2 0 30 32 34 720.2 0 34 33 32 720.2 0 32 34 25 720.2 0 25 35 18 720.2 0 18 36 13 720.2 0 13 37 26 720.2 0 26 38 58 720.2 0 58 39 36 720.2 0 36 40 38 720.2 0 38 41 375 720.2 0 375 42 1127 720.2 0 1127 43 257 720.2 0 257 44 20 720.2 0 20 45 19 720.2 0 19 46 21 720.2 0 21 47 36 720.2 0 36 48 40 720.2 0 40 49 32 720.2 0 32 50 48 720.2 0 48 51 56 720.2 0 56 52 38 720.2 0 38 53 28 720.2 0 28 54 69 720.2 0 69 55 45 720.2 0 45 56 53 720.2 0 53 57 25 720.2 0 25 58 28 720.2 0 28 59 28 720.2 0 28 60 22 720.2 0 22 61 17 720.2 0 17 62 24 720.2 0 24 63 44 720.2 0 44 64 287 720.2 0 287 65 373 720.2 0 373 66 365 720.2 0 365 67 28 720.2 0 28 68 29 720.2 0 29 69 28 720.2 0 28 70 35 720.2 0 35 71 33 720.2 0 33 72 35 720.2 0 35 73 133 720.2 0 133 74 876 720.2 0 876 75 629 720.2 0 629 76 498 720.2 0 498 77 36 720.2 0 36 78 40 720.2 0 40 79 22 720.2 0 22 80 32 720.2 0 32 81 24 720.2 0 24 82 31 720.2 0 31 83 33 720.2 0 33 84 33 720.2 0 33 85 21 720.2 0 21 86 33 720.2 0 33 87 64 720.2 0 64 88 123 720.2 0 123 89 142 720.2 0 142 90 37 720.2 0 37 91 34 720.2 0 34 92 35 720.2 0 35 93 39 720.2 0 39 94 24 720.2 0 24 95 38 720.2 0 38 96 26 720.2 0 26 97 20 720.2 0 20 98 30 720.2 0 30 99 29 720.2 0 29 100 35 720.2 0 35 101 26 720.2 0 26 102 38 720.2 0 38 103 20 720.2 0 20 104 28 720.2 0 28 105 25 720.2 0 25 106 23 720.2 0 23 107 27 720.2 0 27 108 32 720.2 0 32 109 37 720.2 0 37 110 21 720.2 0 21 111 33 720.2 0 33 112 51 720.2 0 51 113 45 720.2 0 45 114 48 720.2 0 48 115 33 720.2 0 33 116 33 720.2 0 33 117 35 720.2 0 35 118 38 720.2 0 38 119 16 720.2 0 16 120 21 720.2 0 21 121 27 720.2 0 27 122 16 720.2 0 16 123 42 720.2 0 42 124 33 720.2 0 33 125 58 720.2 0 58 126 47 720.2 0 47 127 34 720.2 0 34 128 35 720.2 0 35 129 28 720.2 0 28 130 61 720.2 0 61 131 32 720.2 0 32 132 59 720.2 0 59 133 67 720.2 0 67 134 38 720.2 0 38 135 38 720.2 0 38 136 36 720.2 0 36 137 54 720.2 0 54 138 92 720.2 0 92 139 102 720.2 0 102 140 35 720.2 0 35 141 47 720.2 0 47 142 68 720.2 0 68 143 125 720.2 0 125 144 82 720.2 0 82 145 67 720.2 0 67 146 82 720.2 0 82 147 65 720.2 0 65 148 109 720.2 0 109 149 97 720.2 0 97 150 139 720.2 0 139 151 925 720.2 0 925 152 1956 720.2 0 1956 153 1311 720.2 0 1311 154 532 720.2 0 532 155 294 720.2 0 294 156 1138 720.2 0 1138 157 3686 720.2 0 3686 158 2863 720.2 0 2863 159 740 720.2 0 740 160 252 720.2 0 252 161 268 720.2 0 268 162 95 720.2 0 95 163 94 720.2 0 94 164 95 720.2 0 95 165 285 720.2 0 285 166 277 720.2 0 277 167 293 720.2 0 293 168 196 720.2 0 196 169 239 720.2 0 239 170 477 720.2 0 477 171 1072 720.2 0 1072 172 898 720.2 0 898 173 299 720.2 0 299 174 99 720.2 0 99 175 158 720.2 0 158 176 980 720.2 0 980 177 1915 720.2 0 1915 178 1738 720.2 0 1738 179 823 720.2 0 823 180 93 720.2 0 93 181 146 720.2 0 146 182 143 720.2 0 143 183 186 720.2 0 186 184 124 720.2 0 124 185 118 720.2 0 118 186 64 720.2 0 64 187 55 720.2 0 55 188 154 720.2 0 154 189 86 720.2 0 86 190 89 720.2 0 89 191 52 720.2 0 52 192 44 720.2 0 44 193 37 720.2 0 37 194 49 720.2 0 49 195 84 720.2 0 84 196 76 720.2 0 76 197 66 720.2 0 66 198 38 720.2 0 38 199 49 720.2 0 49 200 182 720.2 0 182 201 431 720.2 0 431 202 283 720.2 0 283 203 85 720.2 0 85 204 43 720.2 0 43 205 69 720.2 0 69 206 66 720.2 0 66 207 46 720.2 0 46 208 43 720.2 0 43 209 39 720.2 0 39 210 41 720.2 0 41 211 35 720.2 0 35 212 38 720.2 0 38 213 40 720.2 0 40 214 97 720.2 0 97 215 151 720.2 0 151 216 120 720.2 0 120 217 65 720.2 0 65 218 52 720.2 0 52 219 64 720.2 0 64 220 72 720.2 0 72 221 54 720.2 0 54 222 57 720.2 0 57 223 72 720.2 0 72 224 88 720.2 0 88 225 72 720.2 0 72 226 120 720.2 0 120 227 75 720.2 0 75 228 271 720.2 0 271 229 155 720.2 0 155 230 332 720.2 0 332 231 126 720.2 0 126 232 97 720.2 0 97 233 209 720.2 0 209 234 391 720.2 0 391 235 427 720.2 0 427 236 239 720.2 0 239 237 156 720.2 0 156 238 359 720.2 0 359 239 144 720.2 0 144 240 178 720.2 0 178 241 98 720.2 0 98 242 67 720.2 0 67 243 72 720.2 0 72 244 82 720.2 0 82 245 84 720.2 0 84 246 102 720.2 0 102 247 88 720.2 0 88 248 177 720.2 0 177 249 421 720.2 0 421 250 496 720.2 0 496 251 589 720.2 0 589 252 555 720.2 0 555 253 1612 720.2 0 1612 254 767 720.2 0 767 255 876 720.2 0 876 256 116 720.2 0 116 257 261 720.2 0 261 258 628 720.2 0 628 259 989 720.2 0 989 260 1140 720.2 0 1140 261 4406 720.2 0 4406 262 5745 720.2 0 5745 263 11680 720.2 0 11680 264 8153 720.2 0 8153 265 4828 720.2 0 4828 266 88 720.2 0 88 267 47 720.2 0 47 268 66 720.2 0 66 269 77 720.2 0 77 270 50 720.2 0 50 271 59 720.2 0 59 272 72 720.2 0 72 273 91 720.2 0 91 274 95 720.2 0 95 275 146 720.2 0 146 276 213 720.2 0 213 277 359 720.2 0 359 278 410 720.2 0 410 279 223 720.2 0 223 280 98 720.2 0 98 281 140 720.2 0 140 282 180 720.2 0 180 283 180 720.2 0 180 284 128 720.2 0 128 285 126 720.2 0 126 286 131 720.2 0 131 287 907 720.2 0 907 288 1920 720.2 0 1920 289 1077 720.2 0 1077 290 199 720.2 0 199 291 232 720.2 0 232 292 569 720.2 0 569 293 534 720.2 0 534 294 572 720.2 0 572 295 263 720.2 0 263 296 351 720.2 0 351 297 455 720.2 0 455 298 1540 720.2 0 1540 299 1608 720.2 0 1608 300 2200 720.2 0 2200 301 306 720.2 0 306 Finished in 3.83 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 60,834 Reads with adapters: 13,378 (22.0%) Reads that were too short: 223 (0.4%) Reads written (passing filters): 13,155 (21.6%) Total basepairs processed: 12,967,478 bp Total written (filtered): 3,522,914 bp (27.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13378 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 425 0.0 3 0 0 10 415 16 84 0.0 3 0 73 7 4 17 12349 0.0 3 11581 636 108 24 18 102 0.0 3 6 26 5 65 19 32 0.0 3 6 4 14 8 20 29 0.0 3 0 0 0 29 22 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 1 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 5 0.0 3 0 0 0 5 40 1 0.0 3 0 0 0 1 41 42 0.0 3 0 0 1 41 42 5 0.0 3 0 0 0 5 44 1 0.0 3 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 25 0.0 3 0 0 3 22 67 4 0.0 3 0 0 0 4 68 6 0.0 3 0 0 0 6 73 1 0.0 3 0 0 0 1 75 13 0.0 3 0 0 0 13 84 6 0.0 3 0 0 0 6 85 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 3 0.0 3 0 0 0 3 117 5 0.0 3 0 0 0 5 118 5 0.0 3 0 0 0 5 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 31 0.0 3 0 0 2 29 128 5 0.0 3 0 0 0 5 129 2 0.0 3 0 0 0 2 130 22 0.0 3 0 0 1 21 131 3 0.0 3 0 0 0 3 133 2 0.0 3 0 0 0 2 135 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 145 4 0.0 3 0 0 0 4 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 151 49 0.0 3 0 0 0 49 154 7 0.0 3 0 0 0 7 155 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 202 4 0.0 3 0 0 1 3 203 1 0.0 3 0 0 0 1 211 3 0.0 3 0 0 0 3 212 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 227 1 0.0 3 1 229 3 0.0 3 0 0 0 3 253 7 0.0 3 0 0 1 6 265 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.77 s (59 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 13,155 Reads with adapters: 459 (3.5%) Reads written (passing filters): 13,155 (100.0%) Total basepairs processed: 3,522,914 bp Total written (filtered): 3,521,520 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 459 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.6% C: 13.3% G: 20.9% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 442 205.5 0 442 4 17 51.4 0 17