This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 144.31 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 552,411 (18.7%) Reads that were too short: 83,900 (2.8%) Reads written (passing filters): 468,511 (15.9%) Total basepairs processed: 887,907,860 bp Total written (filtered): 90,548,868 bp (10.2%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 552411 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 901 720.2 0 901 7 9695 720.2 0 9695 8 250 720.2 0 250 9 11171 720.2 0 11171 10 79 720.2 0 79 11 67 720.2 0 67 12 50 720.2 0 50 13 35 720.2 0 35 14 56 720.2 0 56 15 67 720.2 0 67 16 106 720.2 0 106 17 72 720.2 0 72 18 42 720.2 0 42 19 74 720.2 0 74 20 96 720.2 0 96 21 81 720.2 0 81 22 79 720.2 0 79 23 56 720.2 0 56 24 60 720.2 0 60 25 53 720.2 0 53 26 45 720.2 0 45 27 35 720.2 0 35 28 63 720.2 0 63 29 55 720.2 0 55 30 54 720.2 0 54 31 64 720.2 0 64 32 58 720.2 0 58 33 70 720.2 0 70 34 79 720.2 0 79 35 86 720.2 0 86 36 145 720.2 0 145 37 57 720.2 0 57 38 68 720.2 0 68 39 62 720.2 0 62 40 66 720.2 0 66 41 122 720.2 0 122 42 125 720.2 0 125 43 122 720.2 0 122 44 82 720.2 0 82 45 112 720.2 0 112 46 129 720.2 0 129 47 214 720.2 0 214 48 84 720.2 0 84 49 331 720.2 0 331 50 811 720.2 0 811 51 428 720.2 0 428 52 95 720.2 0 95 53 137 720.2 0 137 54 98 720.2 0 98 55 134 720.2 0 134 56 88 720.2 0 88 57 84 720.2 0 84 58 133 720.2 0 133 59 237 720.2 0 237 60 1110 720.2 0 1110 61 2224 720.2 0 2224 62 1494 720.2 0 1494 63 156 720.2 0 156 64 104 720.2 0 104 65 159 720.2 0 159 66 150 720.2 0 150 67 229 720.2 0 229 68 372 720.2 0 372 69 540 720.2 0 540 70 675 720.2 0 675 71 1475 720.2 0 1475 72 2393 720.2 0 2393 73 859 720.2 0 859 74 707 720.2 0 707 75 1263 720.2 0 1263 76 493 720.2 0 493 77 457 720.2 0 457 78 946 720.2 0 946 79 968 720.2 0 968 80 1344 720.2 0 1344 81 1270 720.2 0 1270 82 1821 720.2 0 1821 83 2354 720.2 0 2354 84 3288 720.2 0 3288 85 68445 720.2 0 68445 86 100473 720.2 0 100473 87 98361 720.2 0 98361 88 473 720.2 0 473 89 67 720.2 0 67 90 88 720.2 0 88 91 53 720.2 0 53 92 51 720.2 0 51 93 73 720.2 0 73 94 50 720.2 0 50 95 50 720.2 0 50 96 53 720.2 0 53 97 59 720.2 0 59 98 52 720.2 0 52 99 50 720.2 0 50 100 51 720.2 0 51 101 49 720.2 0 49 102 54 720.2 0 54 103 43 720.2 0 43 104 33 720.2 0 33 105 56 720.2 0 56 106 61 720.2 0 61 107 71 720.2 0 71 108 57 720.2 0 57 109 65 720.2 0 65 110 61 720.2 0 61 111 64 720.2 0 64 112 40 720.2 0 40 113 62 720.2 0 62 114 62 720.2 0 62 115 46 720.2 0 46 116 52 720.2 0 52 117 50 720.2 0 50 118 42 720.2 0 42 119 55 720.2 0 55 120 71 720.2 0 71 121 92 720.2 0 92 122 130 720.2 0 130 123 209 720.2 0 209 124 211 720.2 0 211 125 70 720.2 0 70 126 85 720.2 0 85 127 71 720.2 0 71 128 112 720.2 0 112 129 171 720.2 0 171 130 419 720.2 0 419 131 1090 720.2 0 1090 132 322 720.2 0 322 133 147 720.2 0 147 134 168 720.2 0 168 135 144 720.2 0 144 136 196 720.2 0 196 137 103 720.2 0 103 138 96 720.2 0 96 139 122 720.2 0 122 140 105 720.2 0 105 141 106 720.2 0 106 142 127 720.2 0 127 143 133 720.2 0 133 144 173 720.2 0 173 145 160 720.2 0 160 146 154 720.2 0 154 147 130 720.2 0 130 148 201 720.2 0 201 149 543 720.2 0 543 150 621 720.2 0 621 151 908 720.2 0 908 152 1571 720.2 0 1571 153 5969 720.2 0 5969 154 3210 720.2 0 3210 155 1894 720.2 0 1894 156 369 720.2 0 369 157 113 720.2 0 113 158 132 720.2 0 132 159 145 720.2 0 145 160 193 720.2 0 193 161 337 720.2 0 337 162 623 720.2 0 623 163 206 720.2 0 206 164 321 720.2 0 321 165 438 720.2 0 438 166 219 720.2 0 219 167 272 720.2 0 272 168 442 720.2 0 442 169 394 720.2 0 394 170 591 720.2 0 591 171 619 720.2 0 619 172 852 720.2 0 852 173 1432 720.2 0 1432 174 2000 720.2 0 2000 175 28806 720.2 0 28806 176 55777 720.2 0 55777 177 27608 720.2 0 27608 178 190 720.2 0 190 179 76 720.2 0 76 180 76 720.2 0 76 181 174 720.2 0 174 182 112 720.2 0 112 183 237 720.2 0 237 184 113 720.2 0 113 185 81 720.2 0 81 186 141 720.2 0 141 187 79 720.2 0 79 188 60 720.2 0 60 189 77 720.2 0 77 190 129 720.2 0 129 191 127 720.2 0 127 192 64 720.2 0 64 193 71 720.2 0 71 194 95 720.2 0 95 195 89 720.2 0 89 196 143 720.2 0 143 197 94 720.2 0 94 198 84 720.2 0 84 199 73 720.2 0 73 200 98 720.2 0 98 201 119 720.2 0 119 202 85 720.2 0 85 203 88 720.2 0 88 204 128 720.2 0 128 205 132 720.2 0 132 206 237 720.2 0 237 207 148 720.2 0 148 208 227 720.2 0 227 209 423 720.2 0 423 210 459 720.2 0 459 211 581 720.2 0 581 212 790 720.2 0 790 213 10049 720.2 0 10049 214 13186 720.2 0 13186 215 33101 720.2 0 33101 216 205 720.2 0 205 217 127 720.2 0 127 218 188 720.2 0 188 219 121 720.2 0 121 220 173 720.2 0 173 221 107 720.2 0 107 222 175 720.2 0 175 223 71 720.2 0 71 224 64 720.2 0 64 225 79 720.2 0 79 226 78 720.2 0 78 227 133 720.2 0 133 228 203 720.2 0 203 229 283 720.2 0 283 230 206 720.2 0 206 231 582 720.2 0 582 232 628 720.2 0 628 233 734 720.2 0 734 234 116 720.2 0 116 235 125 720.2 0 125 236 63 720.2 0 63 237 93 720.2 0 93 238 96 720.2 0 96 239 86 720.2 0 86 240 119 720.2 0 119 241 77 720.2 0 77 242 76 720.2 0 76 243 64 720.2 0 64 244 127 720.2 0 127 245 97 720.2 0 97 246 120 720.2 0 120 247 154 720.2 0 154 248 203 720.2 0 203 249 287 720.2 0 287 250 282 720.2 0 282 251 260 720.2 0 260 252 129 720.2 0 129 253 607 720.2 0 607 254 1326 720.2 0 1326 255 1049 720.2 0 1049 256 461 720.2 0 461 257 504 720.2 0 504 258 330 720.2 0 330 259 262 720.2 0 262 260 59 720.2 0 59 261 127 720.2 0 127 262 89 720.2 0 89 263 61 720.2 0 61 264 85 720.2 0 85 265 68 720.2 0 68 266 101 720.2 0 101 267 97 720.2 0 97 268 95 720.2 0 95 269 112 720.2 0 112 270 134 720.2 0 134 271 132 720.2 0 132 272 108 720.2 0 108 273 85 720.2 0 85 274 117 720.2 0 117 275 138 720.2 0 138 276 140 720.2 0 140 277 817 720.2 0 817 278 1128 720.2 0 1128 279 1209 720.2 0 1209 280 176 720.2 0 176 281 148 720.2 0 148 282 168 720.2 0 168 283 108 720.2 0 108 284 131 720.2 0 131 285 660 720.2 0 660 286 869 720.2 0 869 287 717 720.2 0 717 288 272 720.2 0 272 289 276 720.2 0 276 290 245 720.2 0 245 291 121 720.2 0 121 292 171 720.2 0 171 293 294 720.2 0 294 294 280 720.2 0 280 295 372 720.2 0 372 296 306 720.2 0 306 297 644 720.2 0 644 298 647 720.2 0 647 299 778 720.2 0 778 300 419 720.2 0 419 301 292 720.2 0 292 Finished in 26.03 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 468,511 Reads with adapters: 21,405 (4.6%) Reads that were too short: 1,564 (0.3%) Reads written (passing filters): 19,841 (4.2%) Total basepairs processed: 90,548,868 bp Total written (filtered): 4,327,701 bp (4.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21405 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 1 8 16 61 0.0 3 0 52 5 4 17 12524 0.0 3 10492 648 109 1275 18 50 0.0 3 0 42 1 7 19 864 0.0 3 796 41 15 12 20 230 0.0 3 5 3 8 214 21 2 0.0 3 0 0 1 1 22 2 0.0 3 0 0 0 2 23 2 0.0 3 0 0 0 2 30 1 0.0 3 1 31 1 0.0 3 1 34 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 36 3 0.0 3 0 0 0 3 37 501 0.0 3 0 0 2 499 38 119 0.0 3 0 0 5 114 39 8 0.0 3 0 0 0 8 40 4 0.0 3 0 0 0 4 41 25 0.0 3 0 0 1 24 43 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 11 0.0 3 0 0 1 10 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 8 0.0 3 0 0 0 8 53 12 0.0 3 0 0 0 12 54 2 0.0 3 0 0 0 2 56 6 0.0 3 0 0 1 5 57 2 0.0 3 0 0 0 2 58 2 0.0 3 0 0 0 2 59 3 0.0 3 0 0 0 3 60 3 0.0 3 0 0 0 3 61 3 0.0 3 0 0 0 3 62 8 0.0 3 0 0 0 8 64 14 0.0 3 0 0 0 14 65 19 0.0 3 0 0 0 19 66 4 0.0 3 1 0 0 3 67 4 0.0 3 0 0 1 3 68 29 0.0 3 0 1 2 26 69 9 0.0 3 0 0 0 9 70 3 0.0 3 0 0 1 2 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 77 35 0.0 3 0 0 0 35 78 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 0 2 80 3 0.0 3 0 0 0 3 81 25 0.0 3 0 0 0 25 82 10 0.0 3 0 0 0 10 83 4 0.0 3 0 0 0 4 84 6 0.0 3 0 0 0 6 85 9 0.0 3 0 0 0 9 86 2281 0.0 3 0 0 21 2260 87 807 0.0 3 0 0 8 799 88 389 0.0 3 0 0 5 384 89 639 0.0 3 0 0 29 610 90 101 0.0 3 0 0 3 98 91 80 0.0 3 0 0 2 78 92 54 0.0 3 0 0 1 53 93 8 0.0 3 0 0 0 8 94 24 0.0 3 0 0 0 24 95 8 0.0 3 0 0 1 7 96 3 0.0 3 0 0 0 3 97 5 0.0 3 0 0 0 5 98 23 0.0 3 0 0 0 23 99 17 0.0 3 0 0 0 17 100 30 0.0 3 0 0 1 29 101 34 0.0 3 0 0 0 34 102 23 0.0 3 0 0 0 23 103 7 0.0 3 0 0 0 7 104 11 0.0 3 0 0 0 11 105 9 0.0 3 0 0 0 9 106 78 0.0 3 0 0 1 77 107 231 0.0 3 0 0 3 228 108 6 0.0 3 0 0 0 6 109 14 0.0 3 0 0 2 12 110 65 0.0 3 0 0 2 63 111 6 0.0 3 0 0 0 6 112 60 0.0 3 0 0 0 60 113 38 0.0 3 0 0 2 36 114 13 0.0 3 0 0 1 12 115 4 0.0 3 0 0 0 4 116 1 0.0 3 0 0 0 1 118 6 0.0 3 0 0 0 6 119 12 0.0 3 0 0 1 11 120 5 0.0 3 0 0 0 5 121 1 0.0 3 0 0 0 1 123 5 0.0 3 0 0 0 5 124 17 0.0 3 0 0 0 17 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 9 0.0 3 0 0 0 9 131 165 0.0 3 0 0 0 165 132 10 0.0 3 0 0 0 10 133 6 0.0 3 0 0 0 6 134 18 0.0 3 0 0 1 17 135 1 0.0 3 0 0 0 1 137 44 0.0 3 0 0 2 42 138 22 0.0 3 0 0 1 21 139 21 0.0 3 0 0 1 20 140 18 0.0 3 0 0 1 17 141 10 0.0 3 0 0 0 10 142 7 0.0 3 0 0 0 7 143 31 0.0 3 0 0 0 31 145 2 0.0 3 0 0 0 2 146 6 0.0 3 0 0 0 6 147 4 0.0 3 0 0 1 3 148 1 0.0 3 0 0 0 1 149 3 0.0 3 0 0 0 3 150 5 0.0 3 0 0 0 5 151 3 0.0 3 0 0 1 2 152 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 157 16 0.0 3 0 0 2 14 158 23 0.0 3 0 0 0 23 159 2 0.0 3 0 0 0 2 160 5 0.0 3 0 0 0 5 161 7 0.0 3 0 0 1 6 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 10 0.0 3 0 0 1 9 167 26 0.0 3 0 0 1 25 169 2 0.0 3 0 0 0 2 171 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 175 2 0.0 3 0 0 0 2 177 2 0.0 3 0 0 0 2 179 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 182 8 0.0 3 0 0 0 8 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 4 0.0 3 0 0 0 4 186 7 0.0 3 0 0 0 7 187 1 0.0 3 0 0 0 1 188 13 0.0 3 0 0 0 13 189 14 0.0 3 0 0 1 13 190 2 0.0 3 0 0 0 2 191 5 0.0 3 0 0 0 5 192 1 0.0 3 0 0 0 1 193 20 0.0 3 0 0 0 20 194 5 0.0 3 0 0 0 5 196 1 0.0 3 0 0 0 1 197 6 0.0 3 0 0 0 6 198 176 0.0 3 0 0 1 175 199 130 0.0 3 0 0 4 126 200 85 0.0 3 0 0 0 85 201 182 0.0 3 0 0 5 177 202 146 0.0 3 0 0 11 135 203 53 0.0 3 0 1 3 49 204 23 0.0 3 0 0 0 23 205 5 0.0 3 0 0 0 5 206 36 0.0 3 0 0 0 36 207 29 0.0 3 0 0 0 29 208 20 0.0 3 0 0 1 19 209 4 0.0 3 0 0 0 4 210 3 0.0 3 0 0 0 3 211 2 0.0 3 0 0 0 2 212 4 0.0 3 0 0 0 4 213 11 0.0 3 0 0 0 11 214 22 0.0 3 0 0 1 21 215 6 0.0 3 0 0 0 6 216 57 0.0 3 0 0 3 54 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 226 8 0.0 3 0 0 0 8 227 1 0.0 3 0 0 0 1 232 5 0.0 3 0 0 0 5 236 1 0.0 3 0 0 0 1 239 3 0.0 3 0 0 0 3 244 2 0.0 3 0 0 0 2 274 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 1 280 2 0.0 3 0 0 1 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.05 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 19,841 Reads with adapters: 548 (2.8%) Reads written (passing filters): 19,841 (100.0%) Total basepairs processed: 4,327,701 bp Total written (filtered): 4,326,027 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 548 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.7% C: 21.7% G: 22.8% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 522 310.0 0 522 4 23 77.5 0 23 5 2 19.4 0 2 6 1 4.8 0 1