This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 137.11 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 50,408 (1.7%) Reads that were too short: 8,118 (0.3%) Reads written (passing filters): 42,290 (1.4%) Total basepairs processed: 887,907,860 bp Total written (filtered): 11,264,927 bp (1.3%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 50408 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 225 720.2 0 225 7 389 720.2 0 389 8 18876 720.2 0 18876 9 12967 720.2 0 12967 10 112 720.2 0 112 11 2 720.2 0 2 12 1 720.2 0 1 14 5 720.2 0 5 15 2 720.2 0 2 16 1 720.2 0 1 17 2 720.2 0 2 18 4 720.2 0 4 19 4 720.2 0 4 20 2 720.2 0 2 21 13 720.2 0 13 22 4 720.2 0 4 23 10 720.2 0 10 24 3 720.2 0 3 25 9 720.2 0 9 26 1 720.2 0 1 28 3 720.2 0 3 29 7 720.2 0 7 30 8 720.2 0 8 31 8 720.2 0 8 32 5 720.2 0 5 33 26 720.2 0 26 34 23 720.2 0 23 35 21 720.2 0 21 36 40 720.2 0 40 37 12 720.2 0 12 38 48 720.2 0 48 39 5 720.2 0 5 40 50 720.2 0 50 41 4 720.2 0 4 42 2 720.2 0 2 43 3 720.2 0 3 44 5 720.2 0 5 45 15 720.2 0 15 46 7 720.2 0 7 47 1 720.2 0 1 48 5 720.2 0 5 49 4 720.2 0 4 50 6 720.2 0 6 51 10 720.2 0 10 52 12 720.2 0 12 53 7 720.2 0 7 54 6 720.2 0 6 55 11 720.2 0 11 56 2 720.2 0 2 57 6 720.2 0 6 58 77 720.2 0 77 59 60 720.2 0 60 60 45 720.2 0 45 61 4 720.2 0 4 62 17 720.2 0 17 63 12 720.2 0 12 64 7 720.2 0 7 65 4 720.2 0 4 66 7 720.2 0 7 67 9 720.2 0 9 68 12 720.2 0 12 69 14 720.2 0 14 70 50 720.2 0 50 71 116 720.2 0 116 72 13 720.2 0 13 73 30 720.2 0 30 74 15 720.2 0 15 75 17 720.2 0 17 76 89 720.2 0 89 77 526 720.2 0 526 78 239 720.2 0 239 79 16 720.2 0 16 80 233 720.2 0 233 81 144 720.2 0 144 82 232 720.2 0 232 83 16 720.2 0 16 84 6 720.2 0 6 85 11 720.2 0 11 86 7 720.2 0 7 87 23 720.2 0 23 88 12 720.2 0 12 89 24 720.2 0 24 90 22 720.2 0 22 91 9 720.2 0 9 92 4 720.2 0 4 93 13 720.2 0 13 94 13 720.2 0 13 95 21 720.2 0 21 96 15 720.2 0 15 97 10 720.2 0 10 98 15 720.2 0 15 99 37 720.2 0 37 100 391 720.2 0 391 101 598 720.2 0 598 102 1025 720.2 0 1025 103 27 720.2 0 27 104 8 720.2 0 8 105 19 720.2 0 19 106 7 720.2 0 7 107 21 720.2 0 21 108 15 720.2 0 15 109 55 720.2 0 55 110 579 720.2 0 579 111 305 720.2 0 305 112 232 720.2 0 232 113 15 720.2 0 15 114 4 720.2 0 4 115 8 720.2 0 8 116 66 720.2 0 66 117 12 720.2 0 12 118 8 720.2 0 8 119 7 720.2 0 7 120 2 720.2 0 2 121 4 720.2 0 4 122 10 720.2 0 10 123 20 720.2 0 20 124 13 720.2 0 13 125 9 720.2 0 9 126 15 720.2 0 15 127 95 720.2 0 95 128 8 720.2 0 8 129 10 720.2 0 10 130 14 720.2 0 14 131 20 720.2 0 20 132 29 720.2 0 29 133 24 720.2 0 24 134 9 720.2 0 9 135 5 720.2 0 5 136 9 720.2 0 9 137 39 720.2 0 39 138 13 720.2 0 13 139 9 720.2 0 9 140 29 720.2 0 29 141 15 720.2 0 15 142 18 720.2 0 18 143 22 720.2 0 22 144 20 720.2 0 20 145 20 720.2 0 20 146 17 720.2 0 17 147 31 720.2 0 31 148 47 720.2 0 47 149 28 720.2 0 28 150 28 720.2 0 28 151 51 720.2 0 51 152 414 720.2 0 414 153 23 720.2 0 23 154 8 720.2 0 8 155 8 720.2 0 8 156 11 720.2 0 11 157 34 720.2 0 34 158 46 720.2 0 46 159 23 720.2 0 23 160 23 720.2 0 23 161 11 720.2 0 11 162 25 720.2 0 25 163 11 720.2 0 11 164 6 720.2 0 6 165 5 720.2 0 5 166 12 720.2 0 12 167 13 720.2 0 13 168 81 720.2 0 81 169 18 720.2 0 18 170 41 720.2 0 41 171 6 720.2 0 6 172 5 720.2 0 5 173 6 720.2 0 6 174 10 720.2 0 10 175 7 720.2 0 7 176 6 720.2 0 6 177 14 720.2 0 14 178 28 720.2 0 28 179 11 720.2 0 11 180 9 720.2 0 9 181 13 720.2 0 13 182 26 720.2 0 26 183 38 720.2 0 38 184 37 720.2 0 37 185 32 720.2 0 32 186 46 720.2 0 46 187 23 720.2 0 23 188 14 720.2 0 14 189 22 720.2 0 22 190 20 720.2 0 20 191 21 720.2 0 21 192 17 720.2 0 17 193 17 720.2 0 17 194 32 720.2 0 32 195 24 720.2 0 24 196 31 720.2 0 31 197 56 720.2 0 56 198 109 720.2 0 109 199 564 720.2 0 564 200 722 720.2 0 722 201 186 720.2 0 186 202 141 720.2 0 141 203 226 720.2 0 226 204 311 720.2 0 311 205 67 720.2 0 67 206 38 720.2 0 38 207 28 720.2 0 28 208 28 720.2 0 28 209 40 720.2 0 40 210 26 720.2 0 26 211 16 720.2 0 16 212 19 720.2 0 19 213 26 720.2 0 26 214 27 720.2 0 27 215 25 720.2 0 25 216 35 720.2 0 35 217 122 720.2 0 122 218 261 720.2 0 261 219 269 720.2 0 269 220 57 720.2 0 57 221 46 720.2 0 46 222 462 720.2 0 462 223 817 720.2 0 817 224 648 720.2 0 648 225 74 720.2 0 74 226 45 720.2 0 45 227 26 720.2 0 26 228 29 720.2 0 29 229 41 720.2 0 41 230 19 720.2 0 19 231 24 720.2 0 24 232 15 720.2 0 15 233 15 720.2 0 15 234 20 720.2 0 20 235 36 720.2 0 36 236 28 720.2 0 28 237 28 720.2 0 28 238 26 720.2 0 26 239 56 720.2 0 56 240 48 720.2 0 48 241 64 720.2 0 64 242 119 720.2 0 119 243 50 720.2 0 50 244 27 720.2 0 27 245 12 720.2 0 12 246 26 720.2 0 26 247 28 720.2 0 28 248 24 720.2 0 24 249 22 720.2 0 22 250 28 720.2 0 28 251 27 720.2 0 27 252 38 720.2 0 38 253 27 720.2 0 27 254 33 720.2 0 33 255 68 720.2 0 68 256 84 720.2 0 84 257 163 720.2 0 163 258 112 720.2 0 112 259 74 720.2 0 74 260 55 720.2 0 55 261 35 720.2 0 35 262 50 720.2 0 50 263 44 720.2 0 44 264 87 720.2 0 87 265 57 720.2 0 57 266 27 720.2 0 27 267 37 720.2 0 37 268 24 720.2 0 24 269 28 720.2 0 28 270 30 720.2 0 30 271 37 720.2 0 37 272 48 720.2 0 48 273 54 720.2 0 54 274 30 720.2 0 30 275 37 720.2 0 37 276 50 720.2 0 50 277 81 720.2 0 81 278 128 720.2 0 128 279 76 720.2 0 76 280 45 720.2 0 45 281 43 720.2 0 43 282 53 720.2 0 53 283 47 720.2 0 47 284 34 720.2 0 34 285 71 720.2 0 71 286 33 720.2 0 33 287 31 720.2 0 31 288 56 720.2 0 56 289 53 720.2 0 53 290 66 720.2 0 66 291 71 720.2 0 71 292 400 720.2 0 400 293 187 720.2 0 187 294 177 720.2 0 177 295 63 720.2 0 63 296 53 720.2 0 53 297 59 720.2 0 59 298 59 720.2 0 59 299 75 720.2 0 75 300 80 720.2 0 80 301 56 720.2 0 56 Finished in 3.06 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 42,290 Reads with adapters: 19,051 (45.0%) Reads that were too short: 49 (0.1%) Reads written (passing filters): 19,002 (44.9%) Total basepairs processed: 11,264,927 bp Total written (filtered): 5,237,686 bp (46.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19051 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 8 2 16 125 0.0 3 0 101 14 10 17 18680 0.0 3 17646 870 143 21 18 51 0.0 3 3 41 3 4 19 64 0.0 3 13 6 28 17 20 22 0.0 3 1 0 0 21 21 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 1 82 13 0.0 3 0 0 0 13 84 1 0.0 3 0 0 1 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 1 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 111 6 0.0 3 0 0 0 6 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 188 5 0.0 3 0 1 0 4 189 1 0.0 3 0 0 1 193 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 243 2 0.0 3 1 0 0 1 255 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 19,002 Reads with adapters: 193 (1.0%) Reads written (passing filters): 19,002 (100.0%) Total basepairs processed: 5,237,686 bp Total written (filtered): 5,237,105 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 193 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.2% C: 15.0% G: 7.8% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 191 296.9 0 191 4 2 74.2 0 2