This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 138.24 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 255,698 (8.7%) Reads that were too short: 4,003 (0.1%) Reads written (passing filters): 251,695 (8.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 41,974,237 bp (4.7%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 255698 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 211 720.2 0 211 7 8768 720.2 0 8768 8 7493 720.2 0 7493 9 8 720.2 0 8 10 16 720.2 0 16 11 39 720.2 0 39 12 169 720.2 0 169 13 200 720.2 0 200 14 13 720.2 0 13 15 6 720.2 0 6 16 2 720.2 0 2 17 5 720.2 0 5 18 8 720.2 0 8 19 10 720.2 0 10 20 15 720.2 0 15 21 17 720.2 0 17 22 13 720.2 0 13 23 11 720.2 0 11 24 15 720.2 0 15 25 22 720.2 0 22 26 18 720.2 0 18 27 56 720.2 0 56 28 14 720.2 0 14 29 5 720.2 0 5 30 9 720.2 0 9 31 16 720.2 0 16 32 25 720.2 0 25 33 16 720.2 0 16 34 14 720.2 0 14 35 14 720.2 0 14 36 6 720.2 0 6 37 16 720.2 0 16 38 44 720.2 0 44 39 27 720.2 0 27 40 29 720.2 0 29 41 12 720.2 0 12 42 13 720.2 0 13 43 32 720.2 0 32 44 22 720.2 0 22 45 11 720.2 0 11 46 9 720.2 0 9 47 10 720.2 0 10 48 11 720.2 0 11 49 9 720.2 0 9 50 3 720.2 0 3 51 40 720.2 0 40 52 10 720.2 0 10 53 12 720.2 0 12 54 18 720.2 0 18 55 14 720.2 0 14 56 5 720.2 0 5 57 9 720.2 0 9 58 8 720.2 0 8 59 19 720.2 0 19 60 21 720.2 0 21 61 9 720.2 0 9 62 4 720.2 0 4 63 7 720.2 0 7 64 11 720.2 0 11 65 11 720.2 0 11 66 19 720.2 0 19 67 10 720.2 0 10 68 7 720.2 0 7 69 69 720.2 0 69 70 33 720.2 0 33 71 34 720.2 0 34 72 19 720.2 0 19 73 25 720.2 0 25 74 116 720.2 0 116 75 22 720.2 0 22 76 18 720.2 0 18 77 10 720.2 0 10 78 13 720.2 0 13 79 12 720.2 0 12 80 18 720.2 0 18 81 9 720.2 0 9 82 33 720.2 0 33 83 13 720.2 0 13 84 35 720.2 0 35 85 20 720.2 0 20 86 13 720.2 0 13 87 10 720.2 0 10 88 8 720.2 0 8 89 18 720.2 0 18 90 15 720.2 0 15 91 27 720.2 0 27 92 40 720.2 0 40 93 22 720.2 0 22 94 22 720.2 0 22 95 271 720.2 0 271 96 23 720.2 0 23 97 44 720.2 0 44 98 58 720.2 0 58 99 39 720.2 0 39 100 13 720.2 0 13 101 21 720.2 0 21 102 94 720.2 0 94 103 34 720.2 0 34 104 97 720.2 0 97 105 122 720.2 0 122 106 74 720.2 0 74 107 63 720.2 0 63 108 84 720.2 0 84 109 50 720.2 0 50 110 25 720.2 0 25 111 50 720.2 0 50 112 106 720.2 0 106 113 71 720.2 0 71 114 67 720.2 0 67 115 369 720.2 0 369 116 34 720.2 0 34 117 22 720.2 0 22 118 29 720.2 0 29 119 16 720.2 0 16 120 35 720.2 0 35 121 35 720.2 0 35 122 200 720.2 0 200 123 108 720.2 0 108 124 328 720.2 0 328 125 489 720.2 0 489 126 109 720.2 0 109 127 222 720.2 0 222 128 149 720.2 0 149 129 260 720.2 0 260 130 505 720.2 0 505 131 172 720.2 0 172 132 153 720.2 0 153 133 74 720.2 0 74 134 128 720.2 0 128 135 321 720.2 0 321 136 413 720.2 0 413 137 650 720.2 0 650 138 1003 720.2 0 1003 139 1177 720.2 0 1177 140 2759 720.2 0 2759 141 4401 720.2 0 4401 142 84677 720.2 0 84677 143 36399 720.2 0 36399 144 78687 720.2 0 78687 145 344 720.2 0 344 146 51 720.2 0 51 147 54 720.2 0 54 148 33 720.2 0 33 149 56 720.2 0 56 150 74 720.2 0 74 151 24 720.2 0 24 152 26 720.2 0 26 153 10 720.2 0 10 154 24 720.2 0 24 155 41 720.2 0 41 156 46 720.2 0 46 157 102 720.2 0 102 158 113 720.2 0 113 159 105 720.2 0 105 160 246 720.2 0 246 161 339 720.2 0 339 162 6151 720.2 0 6151 163 1851 720.2 0 1851 164 7316 720.2 0 7316 165 42 720.2 0 42 166 10 720.2 0 10 167 20 720.2 0 20 168 19 720.2 0 19 169 36 720.2 0 36 170 10 720.2 0 10 171 13 720.2 0 13 172 11 720.2 0 11 173 10 720.2 0 10 174 9 720.2 0 9 175 16 720.2 0 16 176 24 720.2 0 24 177 57 720.2 0 57 178 34 720.2 0 34 179 26 720.2 0 26 180 9 720.2 0 9 181 7 720.2 0 7 182 19 720.2 0 19 183 13 720.2 0 13 184 29 720.2 0 29 185 22 720.2 0 22 186 14 720.2 0 14 187 29 720.2 0 29 188 210 720.2 0 210 189 51 720.2 0 51 190 86 720.2 0 86 191 10 720.2 0 10 192 11 720.2 0 11 193 16 720.2 0 16 194 8 720.2 0 8 195 11 720.2 0 11 196 14 720.2 0 14 197 17 720.2 0 17 198 10 720.2 0 10 199 16 720.2 0 16 200 14 720.2 0 14 201 16 720.2 0 16 202 20 720.2 0 20 203 15 720.2 0 15 204 12 720.2 0 12 205 12 720.2 0 12 206 21 720.2 0 21 207 29 720.2 0 29 208 24 720.2 0 24 209 38 720.2 0 38 210 48 720.2 0 48 211 50 720.2 0 50 212 56 720.2 0 56 213 35 720.2 0 35 214 88 720.2 0 88 215 73 720.2 0 73 216 115 720.2 0 115 217 49 720.2 0 49 218 40 720.2 0 40 219 14 720.2 0 14 220 15 720.2 0 15 221 24 720.2 0 24 222 39 720.2 0 39 223 61 720.2 0 61 224 85 720.2 0 85 225 140 720.2 0 140 226 33 720.2 0 33 227 50 720.2 0 50 228 11 720.2 0 11 229 12 720.2 0 12 230 13 720.2 0 13 231 22 720.2 0 22 232 44 720.2 0 44 233 71 720.2 0 71 234 66 720.2 0 66 235 12 720.2 0 12 236 23 720.2 0 23 237 20 720.2 0 20 238 36 720.2 0 36 239 32 720.2 0 32 240 42 720.2 0 42 241 21 720.2 0 21 242 24 720.2 0 24 243 37 720.2 0 37 244 27 720.2 0 27 245 20 720.2 0 20 246 29 720.2 0 29 247 29 720.2 0 29 248 16 720.2 0 16 249 17 720.2 0 17 250 18 720.2 0 18 251 15 720.2 0 15 252 20 720.2 0 20 253 26 720.2 0 26 254 23 720.2 0 23 255 26 720.2 0 26 256 42 720.2 0 42 257 33 720.2 0 33 258 35 720.2 0 35 259 50 720.2 0 50 260 48 720.2 0 48 261 47 720.2 0 47 262 60 720.2 0 60 263 98 720.2 0 98 264 68 720.2 0 68 265 30 720.2 0 30 266 27 720.2 0 27 267 22 720.2 0 22 268 33 720.2 0 33 269 59 720.2 0 59 270 55 720.2 0 55 271 41 720.2 0 41 272 17 720.2 0 17 273 17 720.2 0 17 274 40 720.2 0 40 275 32 720.2 0 32 276 83 720.2 0 83 277 67 720.2 0 67 278 53 720.2 0 53 279 71 720.2 0 71 280 68 720.2 0 68 281 117 720.2 0 117 282 44 720.2 0 44 283 58 720.2 0 58 284 50 720.2 0 50 285 52 720.2 0 52 286 29 720.2 0 29 287 35 720.2 0 35 288 23 720.2 0 23 289 21 720.2 0 21 290 21 720.2 0 21 291 24 720.2 0 24 292 18 720.2 0 18 293 25 720.2 0 25 294 26 720.2 0 26 295 40 720.2 0 40 296 146 720.2 0 146 297 47 720.2 0 47 298 24 720.2 0 24 299 24 720.2 0 24 300 27 720.2 0 27 301 18 720.2 0 18 Finished in 13.20 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 251,695 Reads with adapters: 8,825 (3.5%) Reads that were too short: 65 (0.0%) Reads written (passing filters): 8,760 (3.5%) Total basepairs processed: 41,974,237 bp Total written (filtered): 2,421,197 bp (5.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8825 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 9 2 16 202 0.0 3 0 186 14 2 17 8432 0.0 3 8012 354 52 14 18 34 0.0 3 0 21 6 7 19 36 0.0 3 6 2 15 13 20 11 0.0 3 0 0 0 11 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 20 0.0 3 0 0 0 20 60 3 0.0 3 0 1 0 2 63 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 82 2 0.0 3 0 0 1 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 145 2 0.0 3 0 0 0 2 146 9 0.0 3 0 0 0 9 147 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 8,760 Reads with adapters: 135 (1.5%) Reads written (passing filters): 8,760 (100.0%) Total basepairs processed: 2,421,197 bp Total written (filtered): 2,420,777 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 135 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.0% C: 28.1% G: 8.9% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 127 136.9 0 127 4 3 34.2 0 3 5 3 8.6 0 3 6 2 2.1 0 2