This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.46 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 302,288 (10.2%) Reads that were too short: 3,010 (0.1%) Reads written (passing filters): 299,278 (10.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 49,475,045 bp (5.6%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 302288 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 148 720.2 0 148 7 7120 720.2 0 7120 8 9950 720.2 0 9950 9 11 720.2 0 11 10 7 720.2 0 7 11 24 720.2 0 24 12 158 720.2 0 158 13 55 720.2 0 55 14 6 720.2 0 6 15 9 720.2 0 9 16 6 720.2 0 6 17 2 720.2 0 2 18 7 720.2 0 7 19 9 720.2 0 9 20 16 720.2 0 16 21 18 720.2 0 18 22 14 720.2 0 14 23 8 720.2 0 8 24 7 720.2 0 7 25 7 720.2 0 7 26 10 720.2 0 10 27 19 720.2 0 19 28 13 720.2 0 13 29 4 720.2 0 4 30 6 720.2 0 6 31 11 720.2 0 11 32 12 720.2 0 12 33 17 720.2 0 17 34 37 720.2 0 37 35 9 720.2 0 9 36 3 720.2 0 3 37 10 720.2 0 10 38 8 720.2 0 8 39 4 720.2 0 4 40 16 720.2 0 16 41 8 720.2 0 8 42 11 720.2 0 11 43 30 720.2 0 30 44 33 720.2 0 33 45 10 720.2 0 10 46 7 720.2 0 7 47 6 720.2 0 6 48 2 720.2 0 2 49 3 720.2 0 3 50 4 720.2 0 4 51 48 720.2 0 48 52 8 720.2 0 8 53 9 720.2 0 9 54 21 720.2 0 21 55 13 720.2 0 13 56 10 720.2 0 10 57 11 720.2 0 11 58 12 720.2 0 12 59 24 720.2 0 24 60 18 720.2 0 18 61 15 720.2 0 15 62 8 720.2 0 8 63 7 720.2 0 7 64 14 720.2 0 14 65 12 720.2 0 12 66 22 720.2 0 22 67 9 720.2 0 9 68 12 720.2 0 12 69 66 720.2 0 66 70 22 720.2 0 22 71 23 720.2 0 23 72 15 720.2 0 15 73 21 720.2 0 21 74 155 720.2 0 155 75 19 720.2 0 19 76 5 720.2 0 5 77 7 720.2 0 7 78 4 720.2 0 4 79 11 720.2 0 11 80 8 720.2 0 8 81 11 720.2 0 11 82 29 720.2 0 29 83 14 720.2 0 14 84 25 720.2 0 25 85 19 720.2 0 19 86 14 720.2 0 14 87 9 720.2 0 9 88 12 720.2 0 12 89 25 720.2 0 25 90 27 720.2 0 27 91 37 720.2 0 37 92 47 720.2 0 47 93 22 720.2 0 22 94 21 720.2 0 21 95 285 720.2 0 285 96 23 720.2 0 23 97 27 720.2 0 27 98 46 720.2 0 46 99 30 720.2 0 30 100 15 720.2 0 15 101 15 720.2 0 15 102 62 720.2 0 62 103 41 720.2 0 41 104 87 720.2 0 87 105 100 720.2 0 100 106 75 720.2 0 75 107 54 720.2 0 54 108 89 720.2 0 89 109 45 720.2 0 45 110 33 720.2 0 33 111 41 720.2 0 41 112 96 720.2 0 96 113 56 720.2 0 56 114 48 720.2 0 48 115 395 720.2 0 395 116 36 720.2 0 36 117 27 720.2 0 27 118 32 720.2 0 32 119 21 720.2 0 21 120 38 720.2 0 38 121 25 720.2 0 25 122 270 720.2 0 270 123 141 720.2 0 141 124 314 720.2 0 314 125 577 720.2 0 577 126 113 720.2 0 113 127 258 720.2 0 258 128 173 720.2 0 173 129 297 720.2 0 297 130 574 720.2 0 574 131 206 720.2 0 206 132 174 720.2 0 174 133 93 720.2 0 93 134 135 720.2 0 135 135 345 720.2 0 345 136 377 720.2 0 377 137 710 720.2 0 710 138 1104 720.2 0 1104 139 1341 720.2 0 1341 140 2944 720.2 0 2944 141 4535 720.2 0 4535 142 100805 720.2 0 100805 143 42504 720.2 0 42504 144 98609 720.2 0 98609 145 408 720.2 0 408 146 53 720.2 0 53 147 58 720.2 0 58 148 30 720.2 0 30 149 52 720.2 0 52 150 63 720.2 0 63 151 30 720.2 0 30 152 25 720.2 0 25 153 8 720.2 0 8 154 19 720.2 0 19 155 44 720.2 0 44 156 40 720.2 0 40 157 98 720.2 0 98 158 114 720.2 0 114 159 109 720.2 0 109 160 260 720.2 0 260 161 384 720.2 0 384 162 7909 720.2 0 7909 163 2304 720.2 0 2304 164 9208 720.2 0 9208 165 36 720.2 0 36 166 9 720.2 0 9 167 16 720.2 0 16 168 20 720.2 0 20 169 18 720.2 0 18 170 15 720.2 0 15 171 9 720.2 0 9 172 11 720.2 0 11 173 3 720.2 0 3 175 9 720.2 0 9 176 15 720.2 0 15 177 43 720.2 0 43 178 12 720.2 0 12 179 20 720.2 0 20 180 8 720.2 0 8 181 5 720.2 0 5 182 9 720.2 0 9 183 11 720.2 0 11 184 15 720.2 0 15 185 8 720.2 0 8 186 6 720.2 0 6 187 19 720.2 0 19 188 180 720.2 0 180 189 68 720.2 0 68 190 115 720.2 0 115 191 9 720.2 0 9 192 13 720.2 0 13 193 6 720.2 0 6 194 9 720.2 0 9 195 13 720.2 0 13 196 13 720.2 0 13 197 16 720.2 0 16 198 8 720.2 0 8 199 8 720.2 0 8 200 16 720.2 0 16 201 14 720.2 0 14 202 14 720.2 0 14 203 10 720.2 0 10 204 14 720.2 0 14 205 12 720.2 0 12 206 24 720.2 0 24 207 16 720.2 0 16 208 17 720.2 0 17 209 17 720.2 0 17 210 21 720.2 0 21 211 36 720.2 0 36 212 30 720.2 0 30 213 18 720.2 0 18 214 78 720.2 0 78 215 46 720.2 0 46 216 89 720.2 0 89 217 43 720.2 0 43 218 19 720.2 0 19 219 10 720.2 0 10 220 5 720.2 0 5 221 19 720.2 0 19 222 27 720.2 0 27 223 32 720.2 0 32 224 72 720.2 0 72 225 165 720.2 0 165 226 15 720.2 0 15 227 55 720.2 0 55 228 15 720.2 0 15 229 7 720.2 0 7 230 13 720.2 0 13 231 10 720.2 0 10 232 30 720.2 0 30 233 43 720.2 0 43 234 26 720.2 0 26 235 17 720.2 0 17 236 22 720.2 0 22 237 22 720.2 0 22 238 19 720.2 0 19 239 32 720.2 0 32 240 32 720.2 0 32 241 30 720.2 0 30 242 10 720.2 0 10 243 18 720.2 0 18 244 17 720.2 0 17 245 26 720.2 0 26 246 23 720.2 0 23 247 10 720.2 0 10 248 7 720.2 0 7 249 11 720.2 0 11 250 12 720.2 0 12 251 10 720.2 0 10 252 8 720.2 0 8 253 19 720.2 0 19 254 13 720.2 0 13 255 13 720.2 0 13 256 23 720.2 0 23 257 28 720.2 0 28 258 34 720.2 0 34 259 59 720.2 0 59 260 34 720.2 0 34 261 43 720.2 0 43 262 43 720.2 0 43 263 57 720.2 0 57 264 37 720.2 0 37 265 19 720.2 0 19 266 15 720.2 0 15 267 19 720.2 0 19 268 12 720.2 0 12 269 42 720.2 0 42 270 25 720.2 0 25 271 27 720.2 0 27 272 15 720.2 0 15 273 10 720.2 0 10 274 19 720.2 0 19 275 24 720.2 0 24 276 38 720.2 0 38 277 39 720.2 0 39 278 36 720.2 0 36 279 77 720.2 0 77 280 73 720.2 0 73 281 126 720.2 0 126 282 41 720.2 0 41 283 40 720.2 0 40 284 30 720.2 0 30 285 29 720.2 0 29 286 24 720.2 0 24 287 24 720.2 0 24 288 23 720.2 0 23 289 19 720.2 0 19 290 24 720.2 0 24 291 18 720.2 0 18 292 14 720.2 0 14 293 19 720.2 0 19 294 24 720.2 0 24 295 40 720.2 0 40 296 129 720.2 0 129 297 41 720.2 0 41 298 22 720.2 0 22 299 23 720.2 0 23 300 19 720.2 0 19 301 14 720.2 0 14 Finished in 16.83 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 299,278 Reads with adapters: 10,556 (3.5%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 10,551 (3.5%) Total basepairs processed: 49,475,045 bp Total written (filtered): 2,836,485 bp (5.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10556 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 27 0.0 3 0 25 0 0 2 20 10070 0.0 4 9906 161 3 21 33 0.0 4 5 26 2 22 3 0.0 4 1 0 2 23 8 0.0 4 1 0 1 6 35 1 0.0 4 0 0 0 1 36 8 0.0 4 0 0 0 0 8 37 4 0.0 4 0 0 0 0 4 38 3 0.0 4 0 0 0 0 3 123 5 0.0 4 0 0 1 0 4 126 1 0.0 4 0 0 0 0 1 127 386 0.0 4 0 0 0 0 386 128 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 10,551 Reads with adapters: 841 (8.0%) Reads written (passing filters): 10,551 (100.0%) Total basepairs processed: 2,836,485 bp Total written (filtered): 2,820,891 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 841 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.6% G: 91.6% T: 4.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 164.9 0 7 4 4 41.2 0 4 9 6 0.0 0 0 6 10 166 0.0 1 1 165 11 626 0.0 1 7 619 12 1 0.0 1 0 1 224 30 0.0 1 22 8 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.