This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 136.93 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 178,065 (6.0%) Reads that were too short: 23,910 (0.8%) Reads written (passing filters): 154,155 (5.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 40,236,104 bp (4.5%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 178065 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 970 720.2 0 970 7 54011 720.2 0 54011 8 1137 720.2 0 1137 9 59480 720.2 0 59480 10 135 720.2 0 135 11 59 720.2 0 59 12 66 720.2 0 66 13 67 720.2 0 67 14 65 720.2 0 65 15 53 720.2 0 53 16 54 720.2 0 54 17 42 720.2 0 42 18 43 720.2 0 43 19 41 720.2 0 41 20 51 720.2 0 51 21 41 720.2 0 41 22 65 720.2 0 65 23 47 720.2 0 47 24 73 720.2 0 73 25 62 720.2 0 62 26 74 720.2 0 74 27 58 720.2 0 58 28 60 720.2 0 60 29 92 720.2 0 92 30 66 720.2 0 66 31 338 720.2 0 338 32 233 720.2 0 233 33 252 720.2 0 252 34 62 720.2 0 62 35 42 720.2 0 42 36 58 720.2 0 58 37 81 720.2 0 81 38 44 720.2 0 44 39 56 720.2 0 56 40 51 720.2 0 51 41 56 720.2 0 56 42 60 720.2 0 60 43 42 720.2 0 42 44 69 720.2 0 69 45 48 720.2 0 48 46 68 720.2 0 68 47 54 720.2 0 54 48 53 720.2 0 53 49 57 720.2 0 57 50 172 720.2 0 172 51 77 720.2 0 77 52 53 720.2 0 53 53 52 720.2 0 52 54 48 720.2 0 48 55 53 720.2 0 53 56 68 720.2 0 68 57 54 720.2 0 54 58 52 720.2 0 52 59 62 720.2 0 62 60 57 720.2 0 57 61 61 720.2 0 61 62 74 720.2 0 74 63 48 720.2 0 48 64 70 720.2 0 70 65 80 720.2 0 80 66 89 720.2 0 89 67 147 720.2 0 147 68 753 720.2 0 753 69 1426 720.2 0 1426 70 1422 720.2 0 1422 71 62 720.2 0 62 72 50 720.2 0 50 73 37 720.2 0 37 74 51 720.2 0 51 75 115 720.2 0 115 76 85 720.2 0 85 77 53 720.2 0 53 78 59 720.2 0 59 79 177 720.2 0 177 80 114 720.2 0 114 81 154 720.2 0 154 82 70 720.2 0 70 83 59 720.2 0 59 84 59 720.2 0 59 85 54 720.2 0 54 86 47 720.2 0 47 87 62 720.2 0 62 88 86 720.2 0 86 89 67 720.2 0 67 90 43 720.2 0 43 91 84 720.2 0 84 92 59 720.2 0 59 93 63 720.2 0 63 94 78 720.2 0 78 95 73 720.2 0 73 96 91 720.2 0 91 97 78 720.2 0 78 98 118 720.2 0 118 99 130 720.2 0 130 100 100 720.2 0 100 101 73 720.2 0 73 102 66 720.2 0 66 103 84 720.2 0 84 104 53 720.2 0 53 105 69 720.2 0 69 106 51 720.2 0 51 107 82 720.2 0 82 108 62 720.2 0 62 109 106 720.2 0 106 110 72 720.2 0 72 111 89 720.2 0 89 112 84 720.2 0 84 113 277 720.2 0 277 114 66 720.2 0 66 115 69 720.2 0 69 116 334 720.2 0 334 117 260 720.2 0 260 118 164 720.2 0 164 119 105 720.2 0 105 120 120 720.2 0 120 121 116 720.2 0 116 122 137 720.2 0 137 123 104 720.2 0 104 124 107 720.2 0 107 125 95 720.2 0 95 126 106 720.2 0 106 127 114 720.2 0 114 128 87 720.2 0 87 129 107 720.2 0 107 130 144 720.2 0 144 131 204 720.2 0 204 132 172 720.2 0 172 133 633 720.2 0 633 134 3321 720.2 0 3321 135 1154 720.2 0 1154 136 444 720.2 0 444 137 214 720.2 0 214 138 92 720.2 0 92 139 109 720.2 0 109 140 79 720.2 0 79 141 109 720.2 0 109 142 89 720.2 0 89 143 139 720.2 0 139 144 124 720.2 0 124 145 113 720.2 0 113 146 96 720.2 0 96 147 154 720.2 0 154 148 80 720.2 0 80 149 71 720.2 0 71 150 162 720.2 0 162 151 311 720.2 0 311 152 173 720.2 0 173 153 256 720.2 0 256 154 174 720.2 0 174 155 170 720.2 0 170 156 322 720.2 0 322 157 313 720.2 0 313 158 112 720.2 0 112 159 146 720.2 0 146 160 122 720.2 0 122 161 74 720.2 0 74 162 88 720.2 0 88 163 91 720.2 0 91 164 146 720.2 0 146 165 241 720.2 0 241 166 167 720.2 0 167 167 216 720.2 0 216 168 200 720.2 0 200 169 254 720.2 0 254 170 87 720.2 0 87 171 78 720.2 0 78 172 75 720.2 0 75 173 73 720.2 0 73 174 107 720.2 0 107 175 235 720.2 0 235 176 418 720.2 0 418 177 441 720.2 0 441 178 494 720.2 0 494 179 276 720.2 0 276 180 386 720.2 0 386 181 659 720.2 0 659 182 836 720.2 0 836 183 453 720.2 0 453 184 190 720.2 0 190 185 157 720.2 0 157 186 97 720.2 0 97 187 144 720.2 0 144 188 116 720.2 0 116 189 77 720.2 0 77 190 114 720.2 0 114 191 106 720.2 0 106 192 99 720.2 0 99 193 132 720.2 0 132 194 229 720.2 0 229 195 518 720.2 0 518 196 763 720.2 0 763 197 688 720.2 0 688 198 235 720.2 0 235 199 146 720.2 0 146 200 1469 720.2 0 1469 201 3787 720.2 0 3787 202 3039 720.2 0 3039 203 895 720.2 0 895 204 59 720.2 0 59 205 72 720.2 0 72 206 55 720.2 0 55 207 104 720.2 0 104 208 67 720.2 0 67 209 78 720.2 0 78 210 59 720.2 0 59 211 63 720.2 0 63 212 91 720.2 0 91 213 99 720.2 0 99 214 148 720.2 0 148 215 72 720.2 0 72 216 72 720.2 0 72 217 82 720.2 0 82 218 58 720.2 0 58 219 79 720.2 0 79 220 80 720.2 0 80 221 204 720.2 0 204 222 245 720.2 0 245 223 169 720.2 0 169 224 122 720.2 0 122 225 60 720.2 0 60 226 121 720.2 0 121 227 123 720.2 0 123 228 56 720.2 0 56 229 51 720.2 0 51 230 83 720.2 0 83 231 86 720.2 0 86 232 67 720.2 0 67 233 77 720.2 0 77 234 78 720.2 0 78 235 81 720.2 0 81 236 112 720.2 0 112 237 75 720.2 0 75 238 75 720.2 0 75 239 101 720.2 0 101 240 129 720.2 0 129 241 158 720.2 0 158 242 204 720.2 0 204 243 165 720.2 0 165 244 133 720.2 0 133 245 64 720.2 0 64 246 71 720.2 0 71 247 70 720.2 0 70 248 72 720.2 0 72 249 113 720.2 0 113 250 175 720.2 0 175 251 240 720.2 0 240 252 119 720.2 0 119 253 250 720.2 0 250 254 242 720.2 0 242 255 465 720.2 0 465 256 180 720.2 0 180 257 156 720.2 0 156 258 370 720.2 0 370 259 689 720.2 0 689 260 347 720.2 0 347 261 172 720.2 0 172 262 161 720.2 0 161 263 301 720.2 0 301 264 1393 720.2 0 1393 265 1856 720.2 0 1856 266 791 720.2 0 791 267 90 720.2 0 90 268 118 720.2 0 118 269 145 720.2 0 145 270 161 720.2 0 161 271 111 720.2 0 111 272 96 720.2 0 96 273 83 720.2 0 83 274 194 720.2 0 194 275 235 720.2 0 235 276 1302 720.2 0 1302 277 1900 720.2 0 1900 278 918 720.2 0 918 279 64 720.2 0 64 280 67 720.2 0 67 281 57 720.2 0 57 282 76 720.2 0 76 283 85 720.2 0 85 284 68 720.2 0 68 285 68 720.2 0 68 286 55 720.2 0 55 287 64 720.2 0 64 288 75 720.2 0 75 289 111 720.2 0 111 290 106 720.2 0 106 291 78 720.2 0 78 292 100 720.2 0 100 293 93 720.2 0 93 294 67 720.2 0 67 295 77 720.2 0 77 296 86 720.2 0 86 297 106 720.2 0 106 298 148 720.2 0 148 299 182 720.2 0 182 300 121 720.2 0 121 301 259 720.2 0 259 Finished in 10.76 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 154,155 Reads with adapters: 61,994 (40.2%) Reads that were too short: 196 (0.1%) Reads written (passing filters): 61,798 (40.1%) Total basepairs processed: 40,236,104 bp Total written (filtered): 16,956,272 bp (42.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 61994 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 19 0.0 2 0 0 0 19 15 74 0.0 3 0 0 43 31 16 724 0.0 3 0 656 52 16 17 60341 0.0 3 56635 3056 526 124 18 155 0.0 3 17 112 15 11 19 138 0.0 3 21 3 61 53 20 89 0.0 3 1 1 1 86 21 11 0.0 3 11 22 2 0.0 3 0 0 0 2 28 6 0.0 3 0 0 0 6 29 6 0.0 3 0 0 0 6 31 2 0.0 3 0 0 1 1 32 6 0.0 3 0 0 0 6 34 1 0.0 3 0 0 0 1 36 17 0.0 3 1 0 0 16 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 5 0.0 3 0 0 0 5 41 1 0.0 3 0 0 0 1 42 10 0.0 3 0 0 0 10 44 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 47 2 0.0 3 0 0 1 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 7 0.0 3 0 0 0 7 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 4 0.0 3 0 0 0 4 57 96 0.0 3 0 0 1 95 58 1 0.0 3 0 0 0 1 59 5 0.0 3 0 0 0 5 60 7 0.0 3 0 0 0 7 61 2 0.0 3 0 0 0 2 62 6 0.0 3 0 0 0 6 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 75 19 0.0 3 0 0 0 19 78 4 0.0 3 0 0 0 4 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 14 0.0 3 0 0 0 14 93 10 0.0 3 0 0 0 10 94 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 11 0.0 3 0 0 1 10 104 2 0.0 3 0 0 0 2 105 4 0.0 3 0 0 0 4 106 8 0.0 3 0 0 2 6 107 2 0.0 3 0 0 0 2 108 4 0.0 3 0 0 0 4 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 8 0.0 3 0 0 0 8 124 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 137 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 158 3 0.0 3 0 0 0 3 160 18 0.0 3 0 0 0 18 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 5 0.0 3 0 0 0 5 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 176 2 0.0 3 0 1 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 1 1 192 1 0.0 3 0 0 0 1 194 8 0.0 3 0 0 0 8 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 200 1 0.0 3 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 220 3 0.0 3 0 0 0 3 221 3 0.0 3 0 0 0 3 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 1 237 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 267 2 0.0 3 0 0 0 2 277 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 6 0.0 3 0 0 0 6 283 2 0.0 3 0 0 1 1 287 1 0.0 3 0 0 0 1 290 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.45 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 61,798 Reads with adapters: 2,328 (3.8%) Reads written (passing filters): 61,798 (100.0%) Total basepairs processed: 16,956,272 bp Total written (filtered): 16,949,187 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2328 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.8% C: 14.8% G: 16.9% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2244 965.6 0 2244 4 82 241.4 0 82 12 1 0.0 1 0 1 13 1 0.0 1 0 1