This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.55 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 187,348 (6.4%) Reads that were too short: 23,573 (0.8%) Reads written (passing filters): 163,775 (5.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 45,139,001 bp (5.1%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 187348 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 681 720.2 0 681 7 8013 720.2 0 8013 8 446 720.2 0 446 9 6415 720.2 0 6415 10 964 720.2 0 964 11 1093 720.2 0 1093 12 11685 720.2 0 11685 13 64592 720.2 0 64592 14 51942 720.2 0 51942 15 132 720.2 0 132 16 42 720.2 0 42 17 39 720.2 0 39 18 25 720.2 0 25 19 29 720.2 0 29 20 23 720.2 0 23 21 32 720.2 0 32 22 52 720.2 0 52 23 37 720.2 0 37 24 45 720.2 0 45 25 31 720.2 0 31 26 56 720.2 0 56 27 67 720.2 0 67 28 70 720.2 0 70 29 45 720.2 0 45 30 43 720.2 0 43 31 38 720.2 0 38 32 29 720.2 0 29 33 31 720.2 0 31 34 47 720.2 0 47 35 36 720.2 0 36 36 30 720.2 0 30 37 26 720.2 0 26 38 30 720.2 0 30 39 31 720.2 0 31 40 64 720.2 0 64 41 38 720.2 0 38 42 28 720.2 0 28 43 43 720.2 0 43 44 76 720.2 0 76 45 159 720.2 0 159 46 38 720.2 0 38 47 29 720.2 0 29 48 23 720.2 0 23 49 51 720.2 0 51 50 32 720.2 0 32 51 27 720.2 0 27 52 25 720.2 0 25 53 50 720.2 0 50 54 32 720.2 0 32 55 35 720.2 0 35 56 34 720.2 0 34 57 44 720.2 0 44 58 40 720.2 0 40 59 29 720.2 0 29 60 21 720.2 0 21 61 39 720.2 0 39 62 30 720.2 0 30 63 41 720.2 0 41 64 33 720.2 0 33 65 47 720.2 0 47 66 46 720.2 0 46 67 54 720.2 0 54 68 27 720.2 0 27 69 42 720.2 0 42 70 43 720.2 0 43 71 31 720.2 0 31 72 31 720.2 0 31 73 50 720.2 0 50 74 41 720.2 0 41 75 25 720.2 0 25 76 41 720.2 0 41 77 34 720.2 0 34 78 43 720.2 0 43 79 42 720.2 0 42 80 41 720.2 0 41 81 32 720.2 0 32 82 29 720.2 0 29 83 41 720.2 0 41 84 42 720.2 0 42 85 33 720.2 0 33 86 36 720.2 0 36 87 37 720.2 0 37 88 24 720.2 0 24 89 39 720.2 0 39 90 48 720.2 0 48 91 70 720.2 0 70 92 82 720.2 0 82 93 53 720.2 0 53 94 67 720.2 0 67 95 55 720.2 0 55 96 28 720.2 0 28 97 38 720.2 0 38 98 49 720.2 0 49 99 54 720.2 0 54 100 51 720.2 0 51 101 48 720.2 0 48 102 44 720.2 0 44 103 40 720.2 0 40 104 163 720.2 0 163 105 256 720.2 0 256 106 326 720.2 0 326 107 56 720.2 0 56 108 105 720.2 0 105 109 64 720.2 0 64 110 105 720.2 0 105 111 77 720.2 0 77 112 108 720.2 0 108 113 76 720.2 0 76 114 90 720.2 0 90 115 75 720.2 0 75 116 79 720.2 0 79 117 58 720.2 0 58 118 48 720.2 0 48 119 51 720.2 0 51 120 63 720.2 0 63 121 50 720.2 0 50 122 58 720.2 0 58 123 61 720.2 0 61 124 302 720.2 0 302 125 336 720.2 0 336 126 520 720.2 0 520 127 654 720.2 0 654 128 411 720.2 0 411 129 243 720.2 0 243 130 73 720.2 0 73 131 42 720.2 0 42 132 49 720.2 0 49 133 83 720.2 0 83 134 65 720.2 0 65 135 103 720.2 0 103 136 76 720.2 0 76 137 150 720.2 0 150 138 448 720.2 0 448 139 442 720.2 0 442 140 550 720.2 0 550 141 416 720.2 0 416 142 270 720.2 0 270 143 63 720.2 0 63 144 51 720.2 0 51 145 68 720.2 0 68 146 103 720.2 0 103 147 85 720.2 0 85 148 72 720.2 0 72 149 47 720.2 0 47 150 43 720.2 0 43 151 182 720.2 0 182 152 154 720.2 0 154 153 279 720.2 0 279 154 87 720.2 0 87 155 113 720.2 0 113 156 81 720.2 0 81 157 64 720.2 0 64 158 343 720.2 0 343 159 451 720.2 0 451 160 588 720.2 0 588 161 449 720.2 0 449 162 98 720.2 0 98 163 104 720.2 0 104 164 73 720.2 0 73 165 59 720.2 0 59 166 33 720.2 0 33 167 85 720.2 0 85 168 61 720.2 0 61 169 230 720.2 0 230 170 56 720.2 0 56 171 332 720.2 0 332 172 55 720.2 0 55 173 48 720.2 0 48 174 70 720.2 0 70 175 58 720.2 0 58 176 70 720.2 0 70 177 71 720.2 0 71 178 34 720.2 0 34 179 58 720.2 0 58 180 47 720.2 0 47 181 113 720.2 0 113 182 120 720.2 0 120 183 158 720.2 0 158 184 86 720.2 0 86 185 61 720.2 0 61 186 76 720.2 0 76 187 70 720.2 0 70 188 235 720.2 0 235 189 124 720.2 0 124 190 355 720.2 0 355 191 42 720.2 0 42 192 59 720.2 0 59 193 48 720.2 0 48 194 56 720.2 0 56 195 59 720.2 0 59 196 42 720.2 0 42 197 52 720.2 0 52 198 45 720.2 0 45 199 54 720.2 0 54 200 48 720.2 0 48 201 69 720.2 0 69 202 92 720.2 0 92 203 44 720.2 0 44 204 44 720.2 0 44 205 58 720.2 0 58 206 46 720.2 0 46 207 76 720.2 0 76 208 93 720.2 0 93 209 85 720.2 0 85 210 49 720.2 0 49 211 68 720.2 0 68 212 71 720.2 0 71 213 72 720.2 0 72 214 148 720.2 0 148 215 100 720.2 0 100 216 197 720.2 0 197 217 53 720.2 0 53 218 90 720.2 0 90 219 108 720.2 0 108 220 436 720.2 0 436 221 404 720.2 0 404 222 425 720.2 0 425 223 255 720.2 0 255 224 1346 720.2 0 1346 225 971 720.2 0 971 226 3330 720.2 0 3330 227 115 720.2 0 115 228 63 720.2 0 63 229 62 720.2 0 62 230 69 720.2 0 69 231 115 720.2 0 115 232 169 720.2 0 169 233 343 720.2 0 343 234 200 720.2 0 200 235 178 720.2 0 178 236 90 720.2 0 90 237 71 720.2 0 71 238 52 720.2 0 52 239 62 720.2 0 62 240 63 720.2 0 63 241 83 720.2 0 83 242 134 720.2 0 134 243 88 720.2 0 88 244 107 720.2 0 107 245 61 720.2 0 61 246 46 720.2 0 46 247 42 720.2 0 42 248 43 720.2 0 43 249 36 720.2 0 36 250 51 720.2 0 51 251 86 720.2 0 86 252 83 720.2 0 83 253 105 720.2 0 105 254 73 720.2 0 73 255 55 720.2 0 55 256 71 720.2 0 71 257 107 720.2 0 107 258 78 720.2 0 78 259 49 720.2 0 49 260 65 720.2 0 65 261 83 720.2 0 83 262 97 720.2 0 97 263 77 720.2 0 77 264 53 720.2 0 53 265 57 720.2 0 57 266 60 720.2 0 60 267 184 720.2 0 184 268 209 720.2 0 209 269 320 720.2 0 320 270 105 720.2 0 105 271 93 720.2 0 93 272 79 720.2 0 79 273 73 720.2 0 73 274 75 720.2 0 75 275 86 720.2 0 86 276 134 720.2 0 134 277 350 720.2 0 350 278 314 720.2 0 314 279 395 720.2 0 395 280 419 720.2 0 419 281 1288 720.2 0 1288 282 1952 720.2 0 1952 283 2311 720.2 0 2311 284 754 720.2 0 754 285 83 720.2 0 83 286 75 720.2 0 75 287 86 720.2 0 86 288 235 720.2 0 235 289 445 720.2 0 445 290 351 720.2 0 351 291 102 720.2 0 102 292 92 720.2 0 92 293 71 720.2 0 71 294 93 720.2 0 93 295 193 720.2 0 193 296 122 720.2 0 122 297 62 720.2 0 62 298 52 720.2 0 52 299 45 720.2 0 45 300 60 720.2 0 60 301 62 720.2 0 62 Finished in 11.01 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 163,775 Reads with adapters: 10,284 (6.3%) Reads that were too short: 433 (0.3%) Reads written (passing filters): 9,851 (6.0%) Total basepairs processed: 45,139,001 bp Total written (filtered): 2,344,687 bp (5.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10284 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 3 2 16 88 0.0 3 0 79 7 2 17 7128 0.0 3 6795 282 39 12 18 28 0.0 3 3 14 4 7 19 24 0.0 3 0 1 13 10 20 124 0.0 3 103 9 1 11 22 1 0.0 3 0 0 0 1 31 4 0.0 3 1 0 1 2 32 29 0.0 3 0 0 0 29 33 4 0.0 3 1 0 0 3 34 2 0.0 3 0 0 0 2 35 7 0.0 3 0 0 0 7 36 2 0.0 3 0 1 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 7 0.0 3 0 0 0 7 43 12 0.0 3 0 0 0 12 45 4 0.0 3 0 0 1 3 46 1 0.0 3 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 45 0.0 3 0 0 0 45 54 12 0.0 3 0 0 0 12 55 5 0.0 3 0 0 0 5 56 5 0.0 3 0 0 0 5 57 7 0.0 3 0 0 0 7 58 2 0.0 3 0 0 1 1 59 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 1 67 48 0.0 3 0 0 1 47 68 1 0.0 3 0 0 0 1 70 8 0.0 3 0 0 1 7 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 88 8 0.0 3 0 0 0 8 89 2 0.0 3 0 0 0 2 91 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 101 12 0.0 3 0 0 11 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 5 0.0 3 0 0 0 5 110 97 0.0 3 0 0 1 96 111 2 0.0 3 0 0 0 2 112 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 6 0.0 3 0 0 0 6 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 4 0.0 3 0 0 0 4 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 5 0.0 3 0 0 0 5 153 1 0.0 3 0 0 0 1 154 25 0.0 3 0 0 0 25 155 3 0.0 3 0 0 0 3 156 2 0.0 3 0 0 0 2 157 4 0.0 3 0 0 0 4 158 5 0.0 3 0 0 0 5 159 230 0.0 3 0 0 3 227 160 75 0.0 3 0 0 2 73 161 121 0.0 3 0 0 0 121 162 98 0.0 3 0 0 1 97 163 9 0.0 3 0 0 0 9 164 17 0.0 3 0 0 2 15 165 10 0.0 3 0 0 1 9 166 1 0.0 3 0 0 0 1 167 4 0.0 3 0 0 1 3 168 1 0.0 3 0 0 0 1 171 7 0.0 3 0 0 0 7 173 1 0.0 3 0 0 0 1 174 5 0.0 3 0 0 0 5 175 2 0.0 3 0 0 0 2 176 4 0.0 3 0 0 0 4 177 6 0.0 3 0 0 0 6 178 33 0.0 3 0 0 1 32 179 1122 0.0 3 0 0 4 1118 180 99 0.0 3 0 0 1 98 181 8 0.0 3 0 0 0 8 182 135 0.0 3 0 0 11 124 183 20 0.0 3 0 0 3 17 184 1 0.0 3 0 0 0 1 185 8 0.0 3 0 0 0 8 186 6 0.0 3 0 0 0 6 187 1 0.0 3 0 0 0 1 188 9 0.0 3 0 0 0 9 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 3 0.0 3 0 0 0 3 198 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 0 1 203 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 205 7 0.0 3 0 0 0 7 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 5 0.0 3 0 0 0 5 211 3 0.0 3 0 0 0 3 212 6 0.0 3 0 0 0 6 213 4 0.0 3 0 0 0 4 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 230 29 0.0 3 0 0 0 29 231 6 0.0 3 0 0 0 6 232 1 0.0 3 1 233 3 0.0 3 0 0 0 3 235 5 0.0 3 0 0 0 5 238 3 0.0 3 0 0 0 3 239 11 0.0 3 0 0 0 11 240 3 0.0 3 0 0 0 3 242 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 246 3 0.0 3 0 0 0 3 247 1 0.0 3 1 250 2 0.0 3 0 0 0 2 256 1 0.0 3 0 0 0 1 258 2 0.0 3 0 0 0 2 259 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 3 0.0 3 0 0 0 3 262 8 0.0 3 0 0 0 8 263 8 0.0 3 0 0 0 8 264 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 267 2 0.0 3 0 0 0 2 270 4 0.0 3 0 0 0 4 271 53 0.0 3 0 0 0 53 272 14 0.0 3 0 0 1 13 273 18 0.0 3 0 0 0 18 274 28 0.0 3 0 0 2 26 275 24 0.0 3 0 0 3 21 276 13 0.0 3 0 0 1 12 277 14 0.0 3 0 0 1 13 279 7 0.0 3 0 0 0 7 280 49 0.0 3 0 0 1 48 281 17 0.0 3 0 0 0 17 282 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 286 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 289 3 0.0 3 0 0 0 3 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 9,851 Reads with adapters: 118 (1.2%) Reads written (passing filters): 9,851 (100.0%) Total basepairs processed: 2,344,687 bp Total written (filtered): 2,344,318 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 118 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.6% C: 33.9% G: 5.1% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 107 153.9 0 107 4 9 38.5 0 9 6 2 2.4 0 2