This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 137.10 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 150,595 (5.1%) Reads that were too short: 17,846 (0.6%) Reads written (passing filters): 132,749 (4.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 36,804,884 bp (4.1%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 150595 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 588 720.2 0 588 7 6658 720.2 0 6658 8 270 720.2 0 270 9 7982 720.2 0 7982 10 661 720.2 0 661 11 753 720.2 0 753 12 8669 720.2 0 8669 13 50295 720.2 0 50295 14 44169 720.2 0 44169 15 63 720.2 0 63 16 21 720.2 0 21 17 26 720.2 0 26 18 28 720.2 0 28 19 20 720.2 0 20 20 23 720.2 0 23 21 22 720.2 0 22 22 29 720.2 0 29 23 24 720.2 0 24 24 27 720.2 0 27 25 21 720.2 0 21 26 21 720.2 0 21 27 20 720.2 0 20 28 24 720.2 0 24 29 24 720.2 0 24 30 20 720.2 0 20 31 19 720.2 0 19 32 21 720.2 0 21 33 18 720.2 0 18 34 23 720.2 0 23 35 29 720.2 0 29 36 19 720.2 0 19 37 19 720.2 0 19 38 24 720.2 0 24 39 20 720.2 0 20 40 32 720.2 0 32 41 16 720.2 0 16 42 19 720.2 0 19 43 53 720.2 0 53 44 64 720.2 0 64 45 90 720.2 0 90 46 27 720.2 0 27 47 19 720.2 0 19 48 24 720.2 0 24 49 28 720.2 0 28 50 33 720.2 0 33 51 25 720.2 0 25 52 21 720.2 0 21 53 48 720.2 0 48 54 25 720.2 0 25 55 24 720.2 0 24 56 23 720.2 0 23 57 24 720.2 0 24 58 29 720.2 0 29 59 34 720.2 0 34 60 22 720.2 0 22 61 32 720.2 0 32 62 26 720.2 0 26 63 42 720.2 0 42 64 26 720.2 0 26 65 27 720.2 0 27 66 36 720.2 0 36 67 29 720.2 0 29 68 30 720.2 0 30 69 22 720.2 0 22 70 31 720.2 0 31 71 24 720.2 0 24 72 18 720.2 0 18 73 45 720.2 0 45 74 26 720.2 0 26 75 28 720.2 0 28 76 24 720.2 0 24 77 38 720.2 0 38 78 31 720.2 0 31 79 41 720.2 0 41 80 34 720.2 0 34 81 29 720.2 0 29 82 27 720.2 0 27 83 35 720.2 0 35 84 43 720.2 0 43 85 45 720.2 0 45 86 41 720.2 0 41 87 46 720.2 0 46 88 35 720.2 0 35 89 43 720.2 0 43 90 55 720.2 0 55 91 76 720.2 0 76 92 85 720.2 0 85 93 44 720.2 0 44 94 55 720.2 0 55 95 44 720.2 0 44 96 40 720.2 0 40 97 38 720.2 0 38 98 59 720.2 0 59 99 33 720.2 0 33 100 44 720.2 0 44 101 39 720.2 0 39 102 39 720.2 0 39 103 43 720.2 0 43 104 103 720.2 0 103 105 165 720.2 0 165 106 197 720.2 0 197 107 53 720.2 0 53 108 64 720.2 0 64 109 47 720.2 0 47 110 68 720.2 0 68 111 61 720.2 0 61 112 57 720.2 0 57 113 56 720.2 0 56 114 72 720.2 0 72 115 52 720.2 0 52 116 71 720.2 0 71 117 33 720.2 0 33 118 38 720.2 0 38 119 41 720.2 0 41 120 40 720.2 0 40 121 47 720.2 0 47 122 49 720.2 0 49 123 40 720.2 0 40 124 97 720.2 0 97 125 127 720.2 0 127 126 279 720.2 0 279 127 624 720.2 0 624 128 334 720.2 0 334 129 223 720.2 0 223 130 46 720.2 0 46 131 37 720.2 0 37 132 33 720.2 0 33 133 62 720.2 0 62 134 57 720.2 0 57 135 85 720.2 0 85 136 53 720.2 0 53 137 117 720.2 0 117 138 186 720.2 0 186 139 207 720.2 0 207 140 267 720.2 0 267 141 295 720.2 0 295 142 212 720.2 0 212 143 36 720.2 0 36 144 30 720.2 0 30 145 56 720.2 0 56 146 66 720.2 0 66 147 62 720.2 0 62 148 50 720.2 0 50 149 25 720.2 0 25 150 34 720.2 0 34 151 170 720.2 0 170 152 128 720.2 0 128 153 260 720.2 0 260 154 54 720.2 0 54 155 80 720.2 0 80 156 49 720.2 0 49 157 62 720.2 0 62 158 250 720.2 0 250 159 254 720.2 0 254 160 380 720.2 0 380 161 217 720.2 0 217 162 62 720.2 0 62 163 72 720.2 0 72 164 50 720.2 0 50 165 47 720.2 0 47 166 27 720.2 0 27 167 47 720.2 0 47 168 53 720.2 0 53 169 280 720.2 0 280 170 41 720.2 0 41 171 473 720.2 0 473 172 35 720.2 0 35 173 26 720.2 0 26 174 67 720.2 0 67 175 53 720.2 0 53 176 51 720.2 0 51 177 35 720.2 0 35 178 32 720.2 0 32 179 62 720.2 0 62 180 49 720.2 0 49 181 62 720.2 0 62 182 61 720.2 0 61 183 54 720.2 0 54 184 37 720.2 0 37 185 43 720.2 0 43 186 48 720.2 0 48 187 40 720.2 0 40 188 126 720.2 0 126 189 98 720.2 0 98 190 210 720.2 0 210 191 51 720.2 0 51 192 40 720.2 0 40 193 38 720.2 0 38 194 42 720.2 0 42 195 43 720.2 0 43 196 38 720.2 0 38 197 46 720.2 0 46 198 41 720.2 0 41 199 51 720.2 0 51 200 43 720.2 0 43 201 39 720.2 0 39 202 45 720.2 0 45 203 32 720.2 0 32 204 39 720.2 0 39 205 37 720.2 0 37 206 43 720.2 0 43 207 69 720.2 0 69 208 64 720.2 0 64 209 42 720.2 0 42 210 36 720.2 0 36 211 44 720.2 0 44 212 38 720.2 0 38 213 33 720.2 0 33 214 112 720.2 0 112 215 103 720.2 0 103 216 135 720.2 0 135 217 51 720.2 0 51 218 52 720.2 0 52 219 57 720.2 0 57 220 257 720.2 0 257 221 294 720.2 0 294 222 311 720.2 0 311 223 264 720.2 0 264 224 1357 720.2 0 1357 225 974 720.2 0 974 226 3935 720.2 0 3935 227 90 720.2 0 90 228 34 720.2 0 34 229 36 720.2 0 36 230 47 720.2 0 47 231 53 720.2 0 53 232 97 720.2 0 97 233 273 720.2 0 273 234 226 720.2 0 226 235 211 720.2 0 211 236 89 720.2 0 89 237 65 720.2 0 65 238 38 720.2 0 38 239 34 720.2 0 34 240 44 720.2 0 44 241 58 720.2 0 58 242 84 720.2 0 84 243 44 720.2 0 44 244 59 720.2 0 59 245 43 720.2 0 43 246 38 720.2 0 38 247 39 720.2 0 39 248 28 720.2 0 28 249 36 720.2 0 36 250 40 720.2 0 40 251 57 720.2 0 57 252 47 720.2 0 47 253 78 720.2 0 78 254 57 720.2 0 57 255 54 720.2 0 54 256 48 720.2 0 48 257 70 720.2 0 70 258 52 720.2 0 52 259 49 720.2 0 49 260 51 720.2 0 51 261 71 720.2 0 71 262 75 720.2 0 75 263 58 720.2 0 58 264 52 720.2 0 52 265 41 720.2 0 41 266 41 720.2 0 41 267 84 720.2 0 84 268 105 720.2 0 105 269 203 720.2 0 203 270 92 720.2 0 92 271 58 720.2 0 58 272 62 720.2 0 62 273 59 720.2 0 59 274 72 720.2 0 72 275 54 720.2 0 54 276 129 720.2 0 129 277 380 720.2 0 380 278 349 720.2 0 349 279 453 720.2 0 453 280 196 720.2 0 196 281 569 720.2 0 569 282 835 720.2 0 835 283 1133 720.2 0 1133 284 447 720.2 0 447 285 42 720.2 0 42 286 50 720.2 0 50 287 64 720.2 0 64 288 137 720.2 0 137 289 216 720.2 0 216 290 201 720.2 0 201 291 68 720.2 0 68 292 77 720.2 0 77 293 65 720.2 0 65 294 69 720.2 0 69 295 123 720.2 0 123 296 80 720.2 0 80 297 55 720.2 0 55 298 34 720.2 0 34 299 40 720.2 0 40 300 53 720.2 0 53 301 61 720.2 0 61 Finished in 9.87 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 132,749 Reads with adapters: 9,982 (7.5%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 9,936 (7.5%) Total basepairs processed: 36,804,884 bp Total written (filtered): 2,467,400 bp (6.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9982 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 22 0.0 3 0 22 20 7573 0.0 4 7477 93 3 21 27 0.0 4 2 24 0 0 1 22 8 0.0 4 4 0 1 0 3 23 88 0.0 4 83 1 0 4 24 2 0.0 4 1 1 69 1 0.0 4 0 0 0 0 1 78 3 0.0 4 0 0 0 0 3 109 3 0.0 4 0 0 0 0 3 114 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 1 0 1 120 6 0.0 4 0 0 0 0 6 121 10 0.0 4 0 0 0 0 10 122 2177 0.0 4 0 0 0 0 2177 123 4 0.0 4 0 0 0 0 4 124 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 1 142 1 0.0 4 1 146 1 0.0 4 0 0 0 0 1 148 3 0.0 4 0 0 0 0 3 149 1 0.0 4 0 1 167 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 181 1 0.0 4 1 186 1 0.0 4 1 188 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 221 1 0.0 4 1 223 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 3 0.0 4 0 0 0 0 3 228 4 0.0 4 0 0 0 0 4 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 1 235 2 0.0 4 1 0 0 0 1 250 1 0.0 4 0 0 0 0 1 259 3 0.0 4 0 0 0 0 3 260 1 0.0 4 0 0 0 0 1 267 2 0.0 4 0 0 1 0 1 273 1 0.0 4 0 0 0 1 275 1 0.0 4 0 0 0 0 1 277 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.54 s (54 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 9,936 Reads with adapters: 951 (9.6%) Reads written (passing filters): 9,936 (100.0%) Total basepairs processed: 2,467,400 bp Total written (filtered): 2,448,364 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 951 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 5.0% G: 87.3% T: 3.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 38.8 0 1 5 2 9.7 0 2 7 1 0.6 0 1 9 376 0.0 0 13 363 10 481 0.0 1 12 469 11 33 0.0 1 0 33 25 1 0.0 1 1 80 2 0.0 1 1 1 126 1 0.0 1 1 160 30 0.0 1 29 1 223 17 0.0 1 17 226 1 0.0 1 1 266 5 0.0 1 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.