This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.63 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 74,722 (2.5%) Reads that were too short: 13,974 (0.5%) Reads written (passing filters): 60,748 (2.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 15,816,261 bp (1.8%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 74722 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 577 720.2 0 577 7 20077 720.2 0 20077 8 229 720.2 0 229 9 22595 720.2 0 22595 10 71 720.2 0 71 11 98 720.2 0 98 12 25 720.2 0 25 13 42 720.2 0 42 14 38 720.2 0 38 15 46 720.2 0 46 16 38 720.2 0 38 17 51 720.2 0 51 18 30 720.2 0 30 19 38 720.2 0 38 20 46 720.2 0 46 21 41 720.2 0 41 22 50 720.2 0 50 23 42 720.2 0 42 24 43 720.2 0 43 25 46 720.2 0 46 26 33 720.2 0 33 27 27 720.2 0 27 28 25 720.2 0 25 29 69 720.2 0 69 30 36 720.2 0 36 31 38 720.2 0 38 32 39 720.2 0 39 33 36 720.2 0 36 34 35 720.2 0 35 35 36 720.2 0 36 36 31 720.2 0 31 37 47 720.2 0 47 38 42 720.2 0 42 39 47 720.2 0 47 40 40 720.2 0 40 41 36 720.2 0 36 42 70 720.2 0 70 43 28 720.2 0 28 44 43 720.2 0 43 45 39 720.2 0 39 46 46 720.2 0 46 47 31 720.2 0 31 48 35 720.2 0 35 49 48 720.2 0 48 50 31 720.2 0 31 51 24 720.2 0 24 52 49 720.2 0 49 53 56 720.2 0 56 54 44 720.2 0 44 55 56 720.2 0 56 56 49 720.2 0 49 57 46 720.2 0 46 58 57 720.2 0 57 59 35 720.2 0 35 60 44 720.2 0 44 61 36 720.2 0 36 62 27 720.2 0 27 63 34 720.2 0 34 64 41 720.2 0 41 65 50 720.2 0 50 66 34 720.2 0 34 67 53 720.2 0 53 68 65 720.2 0 65 69 46 720.2 0 46 70 57 720.2 0 57 71 67 720.2 0 67 72 34 720.2 0 34 73 129 720.2 0 129 74 79 720.2 0 79 75 138 720.2 0 138 76 44 720.2 0 44 77 39 720.2 0 39 78 38 720.2 0 38 79 50 720.2 0 50 80 50 720.2 0 50 81 45 720.2 0 45 82 49 720.2 0 49 83 37 720.2 0 37 84 48 720.2 0 48 85 49 720.2 0 49 86 45 720.2 0 45 87 60 720.2 0 60 88 45 720.2 0 45 89 41 720.2 0 41 90 44 720.2 0 44 91 49 720.2 0 49 92 41 720.2 0 41 93 34 720.2 0 34 94 39 720.2 0 39 95 51 720.2 0 51 96 58 720.2 0 58 97 53 720.2 0 53 98 31 720.2 0 31 99 67 720.2 0 67 100 62 720.2 0 62 101 520 720.2 0 520 102 296 720.2 0 296 103 422 720.2 0 422 104 69 720.2 0 69 105 88 720.2 0 88 106 54 720.2 0 54 107 45 720.2 0 45 108 52 720.2 0 52 109 35 720.2 0 35 110 66 720.2 0 66 111 323 720.2 0 323 112 249 720.2 0 249 113 505 720.2 0 505 114 89 720.2 0 89 115 99 720.2 0 99 116 125 720.2 0 125 117 92 720.2 0 92 118 42 720.2 0 42 119 57 720.2 0 57 120 48 720.2 0 48 121 50 720.2 0 50 122 47 720.2 0 47 123 104 720.2 0 104 124 106 720.2 0 106 125 98 720.2 0 98 126 82 720.2 0 82 127 57 720.2 0 57 128 48 720.2 0 48 129 40 720.2 0 40 130 69 720.2 0 69 131 94 720.2 0 94 132 411 720.2 0 411 133 114 720.2 0 114 134 232 720.2 0 232 135 43 720.2 0 43 136 78 720.2 0 78 137 140 720.2 0 140 138 150 720.2 0 150 139 133 720.2 0 133 140 75 720.2 0 75 141 140 720.2 0 140 142 116 720.2 0 116 143 149 720.2 0 149 144 445 720.2 0 445 145 260 720.2 0 260 146 646 720.2 0 646 147 68 720.2 0 68 148 112 720.2 0 112 149 626 720.2 0 626 150 88 720.2 0 88 151 121 720.2 0 121 152 60 720.2 0 60 153 46 720.2 0 46 154 192 720.2 0 192 155 94 720.2 0 94 156 256 720.2 0 256 157 67 720.2 0 67 158 115 720.2 0 115 159 93 720.2 0 93 160 103 720.2 0 103 161 86 720.2 0 86 162 58 720.2 0 58 163 83 720.2 0 83 164 102 720.2 0 102 165 66 720.2 0 66 166 88 720.2 0 88 167 51 720.2 0 51 168 107 720.2 0 107 169 131 720.2 0 131 170 365 720.2 0 365 171 207 720.2 0 207 172 389 720.2 0 389 173 42 720.2 0 42 174 43 720.2 0 43 175 55 720.2 0 55 176 46 720.2 0 46 177 74 720.2 0 74 178 140 720.2 0 140 179 178 720.2 0 178 180 152 720.2 0 152 181 73 720.2 0 73 182 57 720.2 0 57 183 48 720.2 0 48 184 63 720.2 0 63 185 78 720.2 0 78 186 68 720.2 0 68 187 73 720.2 0 73 188 52 720.2 0 52 189 57 720.2 0 57 190 58 720.2 0 58 191 40 720.2 0 40 192 51 720.2 0 51 193 50 720.2 0 50 194 62 720.2 0 62 195 59 720.2 0 59 196 44 720.2 0 44 197 69 720.2 0 69 198 81 720.2 0 81 199 98 720.2 0 98 200 63 720.2 0 63 201 67 720.2 0 67 202 72 720.2 0 72 203 80 720.2 0 80 204 154 720.2 0 154 205 158 720.2 0 158 206 134 720.2 0 134 207 75 720.2 0 75 208 67 720.2 0 67 209 79 720.2 0 79 210 235 720.2 0 235 211 116 720.2 0 116 212 168 720.2 0 168 213 73 720.2 0 73 214 80 720.2 0 80 215 53 720.2 0 53 216 61 720.2 0 61 217 58 720.2 0 58 218 69 720.2 0 69 219 90 720.2 0 90 220 60 720.2 0 60 221 54 720.2 0 54 222 65 720.2 0 65 223 96 720.2 0 96 224 166 720.2 0 166 225 189 720.2 0 189 226 191 720.2 0 191 227 230 720.2 0 230 228 150 720.2 0 150 229 108 720.2 0 108 230 71 720.2 0 71 231 56 720.2 0 56 232 60 720.2 0 60 233 59 720.2 0 59 234 64 720.2 0 64 235 67 720.2 0 67 236 72 720.2 0 72 237 72 720.2 0 72 238 85 720.2 0 85 239 81 720.2 0 81 240 77 720.2 0 77 241 68 720.2 0 68 242 80 720.2 0 80 243 76 720.2 0 76 244 161 720.2 0 161 245 217 720.2 0 217 246 172 720.2 0 172 247 270 720.2 0 270 248 138 720.2 0 138 249 145 720.2 0 145 250 54 720.2 0 54 251 58 720.2 0 58 252 59 720.2 0 59 253 57 720.2 0 57 254 70 720.2 0 70 255 73 720.2 0 73 256 98 720.2 0 98 257 165 720.2 0 165 258 169 720.2 0 169 259 155 720.2 0 155 260 109 720.2 0 109 261 93 720.2 0 93 262 95 720.2 0 95 263 77 720.2 0 77 264 203 720.2 0 203 265 204 720.2 0 204 266 349 720.2 0 349 267 92 720.2 0 92 268 82 720.2 0 82 269 66 720.2 0 66 270 79 720.2 0 79 271 80 720.2 0 80 272 124 720.2 0 124 273 183 720.2 0 183 274 121 720.2 0 121 275 86 720.2 0 86 276 72 720.2 0 72 277 114 720.2 0 114 278 102 720.2 0 102 279 95 720.2 0 95 280 126 720.2 0 126 281 110 720.2 0 110 282 162 720.2 0 162 283 203 720.2 0 203 284 587 720.2 0 587 285 754 720.2 0 754 286 1294 720.2 0 1294 287 619 720.2 0 619 288 155 720.2 0 155 289 67 720.2 0 67 290 54 720.2 0 54 291 98 720.2 0 98 292 196 720.2 0 196 293 202 720.2 0 202 294 103 720.2 0 103 295 75 720.2 0 75 296 95 720.2 0 95 297 68 720.2 0 68 298 84 720.2 0 84 299 93 720.2 0 93 300 84 720.2 0 84 301 139 720.2 0 139 Finished in 4.39 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 60,748 Reads with adapters: 23,036 (37.9%) Reads that were too short: 44 (0.1%) Reads written (passing filters): 22,992 (37.8%) Total basepairs processed: 15,816,261 bp Total written (filtered): 6,318,752 bp (40.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23036 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 6 0.0 3 0 0 2 4 16 174 0.0 3 0 161 10 3 17 22648 0.0 3 21259 1166 180 43 18 48 0.0 3 7 36 3 2 19 36 0.0 3 0 1 21 14 20 43 0.0 3 0 0 0 43 21 1 0.0 3 0 0 0 1 22 2 0.0 3 0 0 1 1 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 70 12 0.0 3 0 0 0 12 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 208 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 286 2 0.0 3 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.32 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 22,992 Reads with adapters: 688 (3.0%) Reads written (passing filters): 22,992 (100.0%) Total basepairs processed: 6,318,752 bp Total written (filtered): 6,316,652 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 688 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.4% C: 11.0% G: 28.5% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 653 359.2 0 653 4 34 89.8 0 34 5 1 22.5 0 1