This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.67 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 65,043 (2.2%) Reads that were too short: 8,052 (0.3%) Reads written (passing filters): 56,991 (1.9%) Total basepairs processed: 887,907,860 bp Total written (filtered): 15,369,244 bp (1.7%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 65043 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 579 720.2 0 579 7 26171 720.2 0 26171 8 177 720.2 0 177 9 18460 720.2 0 18460 10 36 720.2 0 36 11 41 720.2 0 41 12 28 720.2 0 28 13 29 720.2 0 29 14 22 720.2 0 22 15 19 720.2 0 19 16 25 720.2 0 25 17 27 720.2 0 27 18 22 720.2 0 22 19 22 720.2 0 22 20 29 720.2 0 29 21 43 720.2 0 43 22 30 720.2 0 30 23 42 720.2 0 42 24 32 720.2 0 32 25 22 720.2 0 22 26 29 720.2 0 29 27 21 720.2 0 21 28 17 720.2 0 17 29 32 720.2 0 32 30 22 720.2 0 22 31 23 720.2 0 23 32 18 720.2 0 18 33 33 720.2 0 33 34 14 720.2 0 14 35 27 720.2 0 27 36 23 720.2 0 23 37 18 720.2 0 18 38 25 720.2 0 25 39 21 720.2 0 21 40 23 720.2 0 23 41 20 720.2 0 20 42 32 720.2 0 32 43 17 720.2 0 17 44 21 720.2 0 21 45 28 720.2 0 28 46 29 720.2 0 29 47 36 720.2 0 36 48 27 720.2 0 27 49 40 720.2 0 40 50 21 720.2 0 21 51 27 720.2 0 27 52 127 720.2 0 127 53 45 720.2 0 45 54 38 720.2 0 38 55 40 720.2 0 40 56 32 720.2 0 32 57 19 720.2 0 19 58 30 720.2 0 30 59 14 720.2 0 14 60 26 720.2 0 26 61 24 720.2 0 24 62 22 720.2 0 22 63 21 720.2 0 21 64 34 720.2 0 34 65 35 720.2 0 35 66 23 720.2 0 23 67 33 720.2 0 33 68 42 720.2 0 42 69 28 720.2 0 28 70 60 720.2 0 60 71 75 720.2 0 75 72 32 720.2 0 32 73 130 720.2 0 130 74 52 720.2 0 52 75 138 720.2 0 138 76 26 720.2 0 26 77 21 720.2 0 21 78 23 720.2 0 23 79 23 720.2 0 23 80 28 720.2 0 28 81 28 720.2 0 28 82 75 720.2 0 75 83 20 720.2 0 20 84 31 720.2 0 31 85 23 720.2 0 23 86 28 720.2 0 28 87 34 720.2 0 34 88 40 720.2 0 40 89 31 720.2 0 31 90 28 720.2 0 28 91 29 720.2 0 29 92 28 720.2 0 28 93 30 720.2 0 30 94 22 720.2 0 22 95 31 720.2 0 31 96 34 720.2 0 34 97 34 720.2 0 34 98 31 720.2 0 31 99 34 720.2 0 34 100 28 720.2 0 28 101 284 720.2 0 284 102 150 720.2 0 150 103 195 720.2 0 195 104 53 720.2 0 53 105 38 720.2 0 38 106 47 720.2 0 47 107 27 720.2 0 27 108 27 720.2 0 27 109 37 720.2 0 37 110 48 720.2 0 48 111 166 720.2 0 166 112 92 720.2 0 92 113 259 720.2 0 259 114 57 720.2 0 57 115 53 720.2 0 53 116 83 720.2 0 83 117 51 720.2 0 51 118 41 720.2 0 41 119 31 720.2 0 31 120 21 720.2 0 21 121 24 720.2 0 24 122 31 720.2 0 31 123 56 720.2 0 56 124 78 720.2 0 78 125 67 720.2 0 67 126 57 720.2 0 57 127 48 720.2 0 48 128 11 720.2 0 11 129 27 720.2 0 27 130 46 720.2 0 46 131 58 720.2 0 58 132 308 720.2 0 308 133 76 720.2 0 76 134 200 720.2 0 200 135 30 720.2 0 30 136 36 720.2 0 36 137 78 720.2 0 78 138 98 720.2 0 98 139 61 720.2 0 61 140 56 720.2 0 56 141 69 720.2 0 69 142 65 720.2 0 65 143 74 720.2 0 74 144 315 720.2 0 315 145 177 720.2 0 177 146 482 720.2 0 482 147 32 720.2 0 32 148 120 720.2 0 120 149 725 720.2 0 725 150 63 720.2 0 63 151 95 720.2 0 95 152 45 720.2 0 45 153 43 720.2 0 43 154 148 720.2 0 148 155 57 720.2 0 57 156 185 720.2 0 185 157 48 720.2 0 48 158 56 720.2 0 56 159 44 720.2 0 44 160 61 720.2 0 61 161 42 720.2 0 42 162 25 720.2 0 25 163 48 720.2 0 48 164 53 720.2 0 53 165 27 720.2 0 27 166 59 720.2 0 59 167 41 720.2 0 41 168 60 720.2 0 60 169 85 720.2 0 85 170 326 720.2 0 326 171 175 720.2 0 175 172 324 720.2 0 324 173 38 720.2 0 38 174 23 720.2 0 23 175 34 720.2 0 34 176 30 720.2 0 30 177 68 720.2 0 68 178 78 720.2 0 78 179 122 720.2 0 122 180 73 720.2 0 73 181 36 720.2 0 36 182 27 720.2 0 27 183 33 720.2 0 33 184 31 720.2 0 31 185 42 720.2 0 42 186 42 720.2 0 42 187 33 720.2 0 33 188 47 720.2 0 47 189 41 720.2 0 41 190 37 720.2 0 37 191 29 720.2 0 29 192 32 720.2 0 32 193 29 720.2 0 29 194 23 720.2 0 23 195 42 720.2 0 42 196 30 720.2 0 30 197 47 720.2 0 47 198 39 720.2 0 39 199 53 720.2 0 53 200 42 720.2 0 42 201 35 720.2 0 35 202 34 720.2 0 34 203 44 720.2 0 44 204 58 720.2 0 58 205 68 720.2 0 68 206 25 720.2 0 25 207 28 720.2 0 28 208 24 720.2 0 24 209 63 720.2 0 63 210 299 720.2 0 299 211 71 720.2 0 71 212 174 720.2 0 174 213 51 720.2 0 51 214 50 720.2 0 50 215 35 720.2 0 35 216 32 720.2 0 32 217 41 720.2 0 41 218 40 720.2 0 40 219 42 720.2 0 42 220 35 720.2 0 35 221 29 720.2 0 29 222 22 720.2 0 22 223 68 720.2 0 68 224 63 720.2 0 63 225 102 720.2 0 102 226 100 720.2 0 100 227 140 720.2 0 140 228 91 720.2 0 91 229 66 720.2 0 66 230 35 720.2 0 35 231 30 720.2 0 30 232 42 720.2 0 42 233 37 720.2 0 37 234 59 720.2 0 59 235 39 720.2 0 39 236 40 720.2 0 40 237 47 720.2 0 47 238 66 720.2 0 66 239 44 720.2 0 44 240 38 720.2 0 38 241 38 720.2 0 38 242 43 720.2 0 43 243 41 720.2 0 41 244 62 720.2 0 62 245 99 720.2 0 99 246 84 720.2 0 84 247 178 720.2 0 178 248 84 720.2 0 84 249 77 720.2 0 77 250 42 720.2 0 42 251 35 720.2 0 35 252 32 720.2 0 32 253 31 720.2 0 31 254 24 720.2 0 24 255 50 720.2 0 50 256 48 720.2 0 48 257 70 720.2 0 70 258 106 720.2 0 106 259 92 720.2 0 92 260 49 720.2 0 49 261 47 720.2 0 47 262 64 720.2 0 64 263 68 720.2 0 68 264 115 720.2 0 115 265 112 720.2 0 112 266 173 720.2 0 173 267 47 720.2 0 47 268 40 720.2 0 40 269 38 720.2 0 38 270 51 720.2 0 51 271 49 720.2 0 49 272 81 720.2 0 81 273 91 720.2 0 91 274 62 720.2 0 62 275 46 720.2 0 46 276 47 720.2 0 47 277 112 720.2 0 112 278 90 720.2 0 90 279 78 720.2 0 78 280 60 720.2 0 60 281 64 720.2 0 64 282 98 720.2 0 98 283 88 720.2 0 88 284 335 720.2 0 335 285 458 720.2 0 458 286 723 720.2 0 723 287 316 720.2 0 316 288 50 720.2 0 50 289 57 720.2 0 57 290 42 720.2 0 42 291 54 720.2 0 54 292 103 720.2 0 103 293 83 720.2 0 83 294 38 720.2 0 38 295 45 720.2 0 45 296 39 720.2 0 39 297 44 720.2 0 44 298 65 720.2 0 65 299 59 720.2 0 59 300 67 720.2 0 67 301 96 720.2 0 96 Finished in 4.42 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 56,991 Reads with adapters: 26,898 (47.2%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 26,886 (47.2%) Total basepairs processed: 15,369,244 bp Total written (filtered): 7,315,877 bp (47.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26898 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 91 0.0 3 0 85 5 1 20 26058 0.0 4 25595 444 13 4 2 21 55 0.0 4 4 51 22 2 0.0 4 0 1 1 23 214 0.0 4 197 5 0 11 1 24 1 0.0 4 0 1 79 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 457 0.0 4 0 0 0 0 457 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.46 s (54 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 26,886 Reads with adapters: 601 (2.2%) Reads written (passing filters): 26,886 (100.0%) Total basepairs processed: 7,315,877 bp Total written (filtered): 7,308,894 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 601 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.6% C: 3.5% G: 84.4% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 31 420.1 0 31 9 14 0.1 0 2 12 10 14 0.0 1 0 14 11 476 0.0 1 5 471 12 34 0.0 1 1 33 20 28 0.0 1 19 9 21 1 0.0 1 1 37 1 0.0 1 1 152 1 0.0 1 0 1 210 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.