This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.34 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 218,960 (7.4%) Reads that were too short: 151,665 (5.1%) Reads written (passing filters): 67,295 (2.3%) Total basepairs processed: 887,907,860 bp Total written (filtered): 16,645,882 bp (1.9%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 218960 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 336 720.2 0 336 7 711 720.2 0 711 8 20180 720.2 0 20180 9 18004 720.2 0 18004 10 34 720.2 0 34 11 23 720.2 0 23 12 117 720.2 0 117 13 24 720.2 0 24 14 7 720.2 0 7 15 22 720.2 0 22 16 18 720.2 0 18 17 16 720.2 0 16 18 15 720.2 0 15 19 16 720.2 0 16 20 21 720.2 0 21 21 13 720.2 0 13 22 15 720.2 0 15 23 21 720.2 0 21 24 29 720.2 0 29 25 21 720.2 0 21 26 20 720.2 0 20 27 28 720.2 0 28 28 36 720.2 0 36 29 16 720.2 0 16 30 16 720.2 0 16 31 17 720.2 0 17 32 28 720.2 0 28 33 30 720.2 0 30 34 18 720.2 0 18 35 17 720.2 0 17 36 25 720.2 0 25 37 18 720.2 0 18 38 27 720.2 0 27 39 28 720.2 0 28 40 22 720.2 0 22 41 14 720.2 0 14 42 37 720.2 0 37 43 101 720.2 0 101 44 444 720.2 0 444 45 59 720.2 0 59 46 34 720.2 0 34 47 15 720.2 0 15 48 25 720.2 0 25 49 25 720.2 0 25 50 47 720.2 0 47 51 28 720.2 0 28 52 20 720.2 0 20 53 17 720.2 0 17 54 29 720.2 0 29 55 34 720.2 0 34 56 104 720.2 0 104 57 270 720.2 0 270 58 141 720.2 0 141 59 26 720.2 0 26 60 39 720.2 0 39 61 59 720.2 0 59 62 40 720.2 0 40 63 21 720.2 0 21 64 46 720.2 0 46 65 35 720.2 0 35 66 19 720.2 0 19 67 48 720.2 0 48 68 64 720.2 0 64 69 496 720.2 0 496 70 247 720.2 0 247 71 892 720.2 0 892 72 44 720.2 0 44 73 49 720.2 0 49 74 50 720.2 0 50 75 63 720.2 0 63 76 102 720.2 0 102 77 41 720.2 0 41 78 78 720.2 0 78 79 63 720.2 0 63 80 32 720.2 0 32 81 37 720.2 0 37 82 42 720.2 0 42 83 44 720.2 0 44 84 60 720.2 0 60 85 47 720.2 0 47 86 57 720.2 0 57 87 75 720.2 0 75 88 117 720.2 0 117 89 1877 720.2 0 1877 90 4660 720.2 0 4660 91 2114 720.2 0 2114 92 34 720.2 0 34 93 28 720.2 0 28 94 31 720.2 0 31 95 32 720.2 0 32 96 44 720.2 0 44 97 145 720.2 0 145 98 122 720.2 0 122 99 116 720.2 0 116 100 70 720.2 0 70 101 32 720.2 0 32 102 65 720.2 0 65 103 28 720.2 0 28 104 35 720.2 0 35 105 42 720.2 0 42 106 26 720.2 0 26 107 37 720.2 0 37 108 45 720.2 0 45 109 48 720.2 0 48 110 34 720.2 0 34 111 77 720.2 0 77 112 55 720.2 0 55 113 41 720.2 0 41 114 23 720.2 0 23 115 17 720.2 0 17 116 23 720.2 0 23 117 27 720.2 0 27 118 23 720.2 0 23 119 19 720.2 0 19 120 34 720.2 0 34 121 22 720.2 0 22 122 37 720.2 0 37 123 27 720.2 0 27 124 48 720.2 0 48 125 29 720.2 0 29 126 23 720.2 0 23 127 96 720.2 0 96 128 25 720.2 0 25 129 28 720.2 0 28 130 23 720.2 0 23 131 40 720.2 0 40 132 100 720.2 0 100 133 51 720.2 0 51 134 82 720.2 0 82 135 72 720.2 0 72 136 115 720.2 0 115 137 1755 720.2 0 1755 138 355 720.2 0 355 139 1858 720.2 0 1858 140 32 720.2 0 32 141 23 720.2 0 23 142 34 720.2 0 34 143 34 720.2 0 34 144 24 720.2 0 24 145 39 720.2 0 39 146 70 720.2 0 70 147 28 720.2 0 28 148 92 720.2 0 92 149 137 720.2 0 137 150 155 720.2 0 155 151 33 720.2 0 33 152 31 720.2 0 31 153 51 720.2 0 51 154 48 720.2 0 48 155 56 720.2 0 56 156 35 720.2 0 35 157 65 720.2 0 65 158 67 720.2 0 67 159 58 720.2 0 58 160 65 720.2 0 65 161 96 720.2 0 96 162 67 720.2 0 67 163 40 720.2 0 40 164 33 720.2 0 33 165 25 720.2 0 25 166 29 720.2 0 29 167 25 720.2 0 25 168 177 720.2 0 177 169 77 720.2 0 77 170 183 720.2 0 183 171 28 720.2 0 28 172 55 720.2 0 55 173 62 720.2 0 62 174 84 720.2 0 84 175 115 720.2 0 115 176 1382 720.2 0 1382 177 509 720.2 0 509 178 3014 720.2 0 3014 179 67 720.2 0 67 180 37 720.2 0 37 181 20 720.2 0 20 182 44 720.2 0 44 183 63 720.2 0 63 184 50 720.2 0 50 185 31 720.2 0 31 186 43 720.2 0 43 187 32 720.2 0 32 188 57 720.2 0 57 189 36 720.2 0 36 190 20 720.2 0 20 191 22 720.2 0 22 192 27 720.2 0 27 193 34 720.2 0 34 194 37 720.2 0 37 195 29 720.2 0 29 196 45 720.2 0 45 197 36 720.2 0 36 198 36 720.2 0 36 199 30 720.2 0 30 200 23 720.2 0 23 201 42 720.2 0 42 202 90 720.2 0 90 203 137 720.2 0 137 204 115 720.2 0 115 205 98 720.2 0 98 206 62 720.2 0 62 207 108 720.2 0 108 208 138 720.2 0 138 209 101 720.2 0 101 210 137 720.2 0 137 211 116 720.2 0 116 212 100 720.2 0 100 213 86 720.2 0 86 214 84 720.2 0 84 215 88 720.2 0 88 216 108 720.2 0 108 217 110 720.2 0 110 218 168 720.2 0 168 219 323 720.2 0 323 220 2985 720.2 0 2985 221 1561 720.2 0 1561 222 3987 720.2 0 3987 223 163 720.2 0 163 224 54 720.2 0 54 225 96 720.2 0 96 226 54 720.2 0 54 227 88 720.2 0 88 228 33 720.2 0 33 229 25 720.2 0 25 230 94 720.2 0 94 231 154 720.2 0 154 232 209 720.2 0 209 233 113 720.2 0 113 234 55 720.2 0 55 235 80 720.2 0 80 236 65 720.2 0 65 237 62 720.2 0 62 238 54 720.2 0 54 239 81 720.2 0 81 240 174 720.2 0 174 241 222 720.2 0 222 242 182 720.2 0 182 243 130 720.2 0 130 244 154 720.2 0 154 245 186 720.2 0 186 246 99 720.2 0 99 247 51 720.2 0 51 248 81 720.2 0 81 249 134 720.2 0 134 250 89 720.2 0 89 251 92 720.2 0 92 252 69 720.2 0 69 253 100 720.2 0 100 254 135 720.2 0 135 255 169 720.2 0 169 256 219 720.2 0 219 257 164 720.2 0 164 258 118 720.2 0 118 259 86 720.2 0 86 260 129 720.2 0 129 261 165 720.2 0 165 262 310 720.2 0 310 263 436 720.2 0 436 264 555 720.2 0 555 265 519 720.2 0 519 266 266 720.2 0 266 267 163 720.2 0 163 268 242 720.2 0 242 269 714 720.2 0 714 270 1473 720.2 0 1473 271 1318 720.2 0 1318 272 424 720.2 0 424 273 289 720.2 0 289 274 2949 720.2 0 2949 275 4418 720.2 0 4418 276 2320 720.2 0 2320 277 246 720.2 0 246 278 429 720.2 0 429 279 625 720.2 0 625 280 831 720.2 0 831 281 1908 720.2 0 1908 282 5895 720.2 0 5895 283 28406 720.2 0 28406 284 30291 720.2 0 30291 285 45722 720.2 0 45722 286 3515 720.2 0 3515 287 65 720.2 0 65 288 260 720.2 0 260 289 393 720.2 0 393 290 680 720.2 0 680 291 337 720.2 0 337 292 103 720.2 0 103 293 36 720.2 0 36 294 29 720.2 0 29 295 43 720.2 0 43 296 51 720.2 0 51 297 75 720.2 0 75 298 83 720.2 0 83 299 49 720.2 0 49 300 39 720.2 0 39 301 28 720.2 0 28 Finished in 4.62 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 67,295 Reads with adapters: 20,807 (30.9%) Reads that were too short: 43 (0.1%) Reads written (passing filters): 20,764 (30.9%) Total basepairs processed: 16,645,882 bp Total written (filtered): 5,700,173 bp (34.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20807 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 19 0.0 3 0 0 13 6 16 186 0.0 3 0 170 9 7 17 20230 0.0 3 19157 915 132 26 18 60 0.0 3 1 44 6 9 19 46 0.0 3 1 0 31 14 20 28 0.0 3 0 0 0 28 32 5 0.0 3 0 0 0 5 35 3 0.0 3 0 0 0 3 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 8 0.0 3 0 0 0 8 44 1 0.0 3 0 0 1 46 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 103 0.0 3 0 0 1 102 102 25 0.0 3 0 0 1 24 104 1 0.0 3 0 0 0 1 105 10 0.0 3 0 0 1 9 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 136 8 0.0 3 0 0 0 8 137 4 0.0 3 0 0 0 4 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 1 1 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 1 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 3 0.0 3 0 0 0 3 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 20,764 Reads with adapters: 221 (1.1%) Reads written (passing filters): 20,764 (100.0%) Total basepairs processed: 5,700,173 bp Total written (filtered): 5,699,408 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 221 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.1% C: 8.1% G: 45.2% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 123 324.4 0 123 4 94 81.1 0 94 5 4 20.3 0 4