This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.82 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 217,939 (7.4%) Reads that were too short: 151,626 (5.1%) Reads written (passing filters): 66,313 (2.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 16,530,346 bp (1.9%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 217939 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 341 720.2 0 341 7 535 720.2 0 535 8 18475 720.2 0 18475 9 21732 720.2 0 21732 10 29 720.2 0 29 11 7 720.2 0 7 12 24 720.2 0 24 13 11 720.2 0 11 14 7 720.2 0 7 15 6 720.2 0 6 16 5 720.2 0 5 17 3 720.2 0 3 18 9 720.2 0 9 19 3 720.2 0 3 20 14 720.2 0 14 21 29 720.2 0 29 22 13 720.2 0 13 23 7 720.2 0 7 24 6 720.2 0 6 25 11 720.2 0 11 26 14 720.2 0 14 27 7 720.2 0 7 28 14 720.2 0 14 29 17 720.2 0 17 30 11 720.2 0 11 31 12 720.2 0 12 32 14 720.2 0 14 33 9 720.2 0 9 34 10 720.2 0 10 35 6 720.2 0 6 36 8 720.2 0 8 37 16 720.2 0 16 38 13 720.2 0 13 39 20 720.2 0 20 40 15 720.2 0 15 41 13 720.2 0 13 42 27 720.2 0 27 43 105 720.2 0 105 44 504 720.2 0 504 45 76 720.2 0 76 46 22 720.2 0 22 47 11 720.2 0 11 48 13 720.2 0 13 49 15 720.2 0 15 50 33 720.2 0 33 51 20 720.2 0 20 52 14 720.2 0 14 53 8 720.2 0 8 54 16 720.2 0 16 55 22 720.2 0 22 56 40 720.2 0 40 57 143 720.2 0 143 58 619 720.2 0 619 59 78 720.2 0 78 60 9 720.2 0 9 61 29 720.2 0 29 62 21 720.2 0 21 63 9 720.2 0 9 64 21 720.2 0 21 65 18 720.2 0 18 66 12 720.2 0 12 67 22 720.2 0 22 68 30 720.2 0 30 69 164 720.2 0 164 70 121 720.2 0 121 71 320 720.2 0 320 72 16 720.2 0 16 73 34 720.2 0 34 74 28 720.2 0 28 75 40 720.2 0 40 76 91 720.2 0 91 77 17 720.2 0 17 78 38 720.2 0 38 79 54 720.2 0 54 80 27 720.2 0 27 81 19 720.2 0 19 82 42 720.2 0 42 83 26 720.2 0 26 84 36 720.2 0 36 85 50 720.2 0 50 86 47 720.2 0 47 87 59 720.2 0 59 88 75 720.2 0 75 89 1556 720.2 0 1556 90 4515 720.2 0 4515 91 2045 720.2 0 2045 92 22 720.2 0 22 93 22 720.2 0 22 94 20 720.2 0 20 95 10 720.2 0 10 96 10 720.2 0 10 97 59 720.2 0 59 98 42 720.2 0 42 99 48 720.2 0 48 100 37 720.2 0 37 101 28 720.2 0 28 102 42 720.2 0 42 103 10 720.2 0 10 104 23 720.2 0 23 105 21 720.2 0 21 106 10 720.2 0 10 107 24 720.2 0 24 108 23 720.2 0 23 109 22 720.2 0 22 110 23 720.2 0 23 111 43 720.2 0 43 112 35 720.2 0 35 113 13 720.2 0 13 114 8 720.2 0 8 115 11 720.2 0 11 116 8 720.2 0 8 117 12 720.2 0 12 118 14 720.2 0 14 119 28 720.2 0 28 120 27 720.2 0 27 121 16 720.2 0 16 122 25 720.2 0 25 123 16 720.2 0 16 124 34 720.2 0 34 125 23 720.2 0 23 126 15 720.2 0 15 127 65 720.2 0 65 128 26 720.2 0 26 129 17 720.2 0 17 130 10 720.2 0 10 131 18 720.2 0 18 132 56 720.2 0 56 133 47 720.2 0 47 134 37 720.2 0 37 135 80 720.2 0 80 136 92 720.2 0 92 137 1835 720.2 0 1835 138 355 720.2 0 355 139 1871 720.2 0 1871 140 22 720.2 0 22 141 9 720.2 0 9 142 15 720.2 0 15 143 18 720.2 0 18 144 25 720.2 0 25 145 11 720.2 0 11 146 10 720.2 0 10 147 9 720.2 0 9 148 37 720.2 0 37 149 59 720.2 0 59 150 66 720.2 0 66 151 15 720.2 0 15 152 5 720.2 0 5 153 22 720.2 0 22 154 21 720.2 0 21 155 28 720.2 0 28 156 22 720.2 0 22 157 25 720.2 0 25 158 35 720.2 0 35 159 42 720.2 0 42 160 39 720.2 0 39 161 64 720.2 0 64 162 40 720.2 0 40 163 34 720.2 0 34 164 16 720.2 0 16 165 13 720.2 0 13 166 17 720.2 0 17 167 34 720.2 0 34 168 194 720.2 0 194 169 64 720.2 0 64 170 211 720.2 0 211 171 23 720.2 0 23 172 57 720.2 0 57 173 55 720.2 0 55 174 74 720.2 0 74 175 100 720.2 0 100 176 1740 720.2 0 1740 177 574 720.2 0 574 178 3835 720.2 0 3835 179 33 720.2 0 33 180 16 720.2 0 16 181 16 720.2 0 16 182 18 720.2 0 18 183 18 720.2 0 18 184 19 720.2 0 19 185 19 720.2 0 19 186 30 720.2 0 30 187 13 720.2 0 13 188 28 720.2 0 28 189 27 720.2 0 27 190 21 720.2 0 21 191 13 720.2 0 13 192 24 720.2 0 24 193 33 720.2 0 33 194 14 720.2 0 14 195 23 720.2 0 23 196 26 720.2 0 26 197 13 720.2 0 13 198 23 720.2 0 23 199 14 720.2 0 14 200 17 720.2 0 17 201 19 720.2 0 19 202 58 720.2 0 58 203 80 720.2 0 80 204 45 720.2 0 45 205 38 720.2 0 38 206 44 720.2 0 44 207 71 720.2 0 71 208 111 720.2 0 111 209 63 720.2 0 63 210 61 720.2 0 61 211 44 720.2 0 44 212 37 720.2 0 37 213 40 720.2 0 40 214 30 720.2 0 30 215 71 720.2 0 71 216 85 720.2 0 85 217 113 720.2 0 113 218 132 720.2 0 132 219 263 720.2 0 263 220 3478 720.2 0 3478 221 1384 720.2 0 1384 222 3953 720.2 0 3953 223 120 720.2 0 120 224 26 720.2 0 26 225 79 720.2 0 79 226 31 720.2 0 31 227 57 720.2 0 57 228 16 720.2 0 16 229 23 720.2 0 23 230 46 720.2 0 46 231 75 720.2 0 75 232 77 720.2 0 77 233 41 720.2 0 41 234 39 720.2 0 39 235 41 720.2 0 41 236 35 720.2 0 35 237 33 720.2 0 33 238 31 720.2 0 31 239 65 720.2 0 65 240 97 720.2 0 97 241 105 720.2 0 105 242 89 720.2 0 89 243 67 720.2 0 67 244 120 720.2 0 120 245 105 720.2 0 105 246 85 720.2 0 85 247 38 720.2 0 38 248 54 720.2 0 54 249 87 720.2 0 87 250 73 720.2 0 73 251 88 720.2 0 88 252 35 720.2 0 35 253 81 720.2 0 81 254 109 720.2 0 109 255 110 720.2 0 110 256 184 720.2 0 184 257 118 720.2 0 118 258 107 720.2 0 107 259 49 720.2 0 49 260 118 720.2 0 118 261 163 720.2 0 163 262 137 720.2 0 137 263 167 720.2 0 167 264 219 720.2 0 219 265 206 720.2 0 206 266 217 720.2 0 217 267 124 720.2 0 124 268 147 720.2 0 147 269 470 720.2 0 470 270 818 720.2 0 818 271 837 720.2 0 837 272 287 720.2 0 287 273 255 720.2 0 255 274 3937 720.2 0 3937 275 5894 720.2 0 5894 276 3117 720.2 0 3117 277 200 720.2 0 200 278 343 720.2 0 343 279 648 720.2 0 648 280 690 720.2 0 690 281 1984 720.2 0 1984 282 4401 720.2 0 4401 283 26586 720.2 0 26586 284 31679 720.2 0 31679 285 51336 720.2 0 51336 286 2480 720.2 0 2480 287 64 720.2 0 64 288 128 720.2 0 128 289 190 720.2 0 190 290 338 720.2 0 338 291 173 720.2 0 173 292 112 720.2 0 112 293 34 720.2 0 34 294 35 720.2 0 35 295 98 720.2 0 98 296 20 720.2 0 20 297 43 720.2 0 43 298 32 720.2 0 32 299 32 720.2 0 32 300 24 720.2 0 24 301 6 720.2 0 6 Finished in 4.93 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 66,313 Reads with adapters: 22,862 (34.5%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 22,814 (34.4%) Total basepairs processed: 16,530,346 bp Total written (filtered): 6,159,933 bp (37.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22862 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 0 1 19 64 0.0 3 0 57 3 4 20 22240 0.0 4 21868 356 14 1 1 21 43 0.0 4 6 33 3 0 1 22 2 0.0 4 1 0 1 23 23 0.0 4 0 0 0 19 4 24 4 0.0 4 0 0 0 0 4 25 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 1 71 1 0.0 4 0 0 0 0 1 106 1 0.0 4 1 110 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 426 0.0 4 0 0 0 0 426 131 3 0.0 4 0 0 0 0 3 136 1 0.0 4 1 137 1 0.0 4 1 146 22 0.0 4 0 0 0 0 22 147 10 0.0 4 0 0 0 1 9 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 200 1 0.0 4 1 213 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.35 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 22,814 Reads with adapters: 4,513 (19.8%) Reads written (passing filters): 22,814 (100.0%) Total basepairs processed: 6,159,933 bp Total written (filtered): 6,116,859 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4513 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 1.7% G: 96.5% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 356.5 0 3 4 3 89.1 0 3 5 1 22.3 0 1 8 2 0.3 0 2 9 3381 0.1 0 75 3306 10 966 0.0 1 37 929 11 142 0.0 1 3 139 12 6 0.0 1 0 6 13 2 0.0 1 0 2 25 1 0.0 1 1 115 1 0.0 1 0 1 208 1 0.0 1 0 1 223 3 0.0 1 3 266 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.