This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.83 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 291,773 (9.9%) Reads that were too short: 26,135 (0.9%) Reads written (passing filters): 265,638 (9.0%) Total basepairs processed: 887,907,860 bp Total written (filtered): 58,607,180 bp (6.6%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 291773 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1262 720.2 0 1262 7 59350 720.2 0 59350 8 48377 720.2 0 48377 9 95 720.2 0 95 10 48 720.2 0 48 11 68 720.2 0 68 12 44 720.2 0 44 13 44 720.2 0 44 14 47 720.2 0 47 15 54 720.2 0 54 16 45 720.2 0 45 17 64 720.2 0 64 18 98 720.2 0 98 19 95 720.2 0 95 20 72 720.2 0 72 21 58 720.2 0 58 22 49 720.2 0 49 23 62 720.2 0 62 24 64 720.2 0 64 25 32 720.2 0 32 26 53 720.2 0 53 27 52 720.2 0 52 28 53 720.2 0 53 29 86 720.2 0 86 30 60 720.2 0 60 31 57 720.2 0 57 32 52 720.2 0 52 33 79 720.2 0 79 34 78 720.2 0 78 35 54 720.2 0 54 36 76 720.2 0 76 37 66 720.2 0 66 38 55 720.2 0 55 39 55 720.2 0 55 40 57 720.2 0 57 41 59 720.2 0 59 42 56 720.2 0 56 43 56 720.2 0 56 44 40 720.2 0 40 45 58 720.2 0 58 46 55 720.2 0 55 47 59 720.2 0 59 48 64 720.2 0 64 49 159 720.2 0 159 50 76 720.2 0 76 51 57 720.2 0 57 52 61 720.2 0 61 53 66 720.2 0 66 54 62 720.2 0 62 55 60 720.2 0 60 56 116 720.2 0 116 57 57 720.2 0 57 58 69 720.2 0 69 59 64 720.2 0 64 60 98 720.2 0 98 61 58 720.2 0 58 62 90 720.2 0 90 63 105 720.2 0 105 64 120 720.2 0 120 65 159 720.2 0 159 66 267 720.2 0 267 67 525 720.2 0 525 68 255 720.2 0 255 69 211 720.2 0 211 70 346 720.2 0 346 71 144 720.2 0 144 72 186 720.2 0 186 73 233 720.2 0 233 74 202 720.2 0 202 75 336 720.2 0 336 76 384 720.2 0 384 77 475 720.2 0 475 78 683 720.2 0 683 79 1333 720.2 0 1333 80 14544 720.2 0 14544 81 28460 720.2 0 28460 82 22962 720.2 0 22962 83 156 720.2 0 156 84 156 720.2 0 156 85 87 720.2 0 87 86 77 720.2 0 77 87 70 720.2 0 70 88 144 720.2 0 144 89 82 720.2 0 82 90 41 720.2 0 41 91 50 720.2 0 50 92 51 720.2 0 51 93 50 720.2 0 50 94 89 720.2 0 89 95 177 720.2 0 177 96 1371 720.2 0 1371 97 44 720.2 0 44 98 76 720.2 0 76 99 77 720.2 0 77 100 39 720.2 0 39 101 35 720.2 0 35 102 67 720.2 0 67 103 63 720.2 0 63 104 41 720.2 0 41 105 41 720.2 0 41 106 50 720.2 0 50 107 40 720.2 0 40 108 47 720.2 0 47 109 35 720.2 0 35 110 51 720.2 0 51 111 96 720.2 0 96 112 66 720.2 0 66 113 67 720.2 0 67 114 52 720.2 0 52 115 33 720.2 0 33 116 61 720.2 0 61 117 70 720.2 0 70 118 123 720.2 0 123 119 245 720.2 0 245 120 225 720.2 0 225 121 389 720.2 0 389 122 342 720.2 0 342 123 78 720.2 0 78 124 147 720.2 0 147 125 151 720.2 0 151 126 133 720.2 0 133 127 260 720.2 0 260 128 381 720.2 0 381 129 508 720.2 0 508 130 111 720.2 0 111 131 162 720.2 0 162 132 73 720.2 0 73 133 76 720.2 0 76 134 77 720.2 0 77 135 87 720.2 0 87 136 45 720.2 0 45 137 80 720.2 0 80 138 57 720.2 0 57 139 82 720.2 0 82 140 76 720.2 0 76 141 81 720.2 0 81 142 81 720.2 0 81 143 104 720.2 0 104 144 240 720.2 0 240 145 204 720.2 0 204 146 537 720.2 0 537 147 104 720.2 0 104 148 149 720.2 0 149 149 224 720.2 0 224 150 107 720.2 0 107 151 43 720.2 0 43 152 67 720.2 0 67 153 84 720.2 0 84 154 64 720.2 0 64 155 70 720.2 0 70 156 46 720.2 0 46 157 38 720.2 0 38 158 50 720.2 0 50 159 49 720.2 0 49 160 49 720.2 0 49 161 61 720.2 0 61 162 81 720.2 0 81 163 61 720.2 0 61 164 105 720.2 0 105 165 73 720.2 0 73 166 80 720.2 0 80 167 168 720.2 0 168 168 113 720.2 0 113 169 86 720.2 0 86 170 72 720.2 0 72 171 150 720.2 0 150 172 98 720.2 0 98 173 102 720.2 0 102 174 199 720.2 0 199 175 375 720.2 0 375 176 158 720.2 0 158 177 181 720.2 0 181 178 219 720.2 0 219 179 135 720.2 0 135 180 156 720.2 0 156 181 243 720.2 0 243 182 327 720.2 0 327 183 338 720.2 0 338 184 511 720.2 0 511 185 521 720.2 0 521 186 681 720.2 0 681 187 724 720.2 0 724 188 7047 720.2 0 7047 189 43064 720.2 0 43064 190 12165 720.2 0 12165 191 839 720.2 0 839 192 526 720.2 0 526 193 327 720.2 0 327 194 308 720.2 0 308 195 126 720.2 0 126 196 258 720.2 0 258 197 106 720.2 0 106 198 61 720.2 0 61 199 72 720.2 0 72 200 62 720.2 0 62 201 177 720.2 0 177 202 363 720.2 0 363 203 75 720.2 0 75 204 136 720.2 0 136 205 145 720.2 0 145 206 2034 720.2 0 2034 207 1078 720.2 0 1078 208 1480 720.2 0 1480 209 101 720.2 0 101 210 133 720.2 0 133 211 214 720.2 0 214 212 696 720.2 0 696 213 702 720.2 0 702 214 284 720.2 0 284 215 81 720.2 0 81 216 52 720.2 0 52 217 41 720.2 0 41 218 44 720.2 0 44 219 64 720.2 0 64 220 48 720.2 0 48 221 51 720.2 0 51 222 116 720.2 0 116 223 150 720.2 0 150 224 47 720.2 0 47 225 104 720.2 0 104 226 103 720.2 0 103 227 174 720.2 0 174 228 72 720.2 0 72 229 136 720.2 0 136 230 213 720.2 0 213 231 125 720.2 0 125 232 66 720.2 0 66 233 105 720.2 0 105 234 94 720.2 0 94 235 80 720.2 0 80 236 65 720.2 0 65 237 76 720.2 0 76 238 45 720.2 0 45 239 78 720.2 0 78 240 83 720.2 0 83 241 96 720.2 0 96 242 96 720.2 0 96 243 60 720.2 0 60 244 65 720.2 0 65 245 66 720.2 0 66 246 70 720.2 0 70 247 121 720.2 0 121 248 94 720.2 0 94 249 72 720.2 0 72 250 55 720.2 0 55 251 77 720.2 0 77 252 119 720.2 0 119 253 106 720.2 0 106 254 88 720.2 0 88 255 123 720.2 0 123 256 170 720.2 0 170 257 194 720.2 0 194 258 161 720.2 0 161 259 132 720.2 0 132 260 113 720.2 0 113 261 99 720.2 0 99 262 107 720.2 0 107 263 95 720.2 0 95 264 90 720.2 0 90 265 69 720.2 0 69 266 82 720.2 0 82 267 116 720.2 0 116 268 233 720.2 0 233 269 444 720.2 0 444 270 393 720.2 0 393 271 857 720.2 0 857 272 2339 720.2 0 2339 273 1622 720.2 0 1622 274 980 720.2 0 980 275 109 720.2 0 109 276 66 720.2 0 66 277 75 720.2 0 75 278 104 720.2 0 104 279 111 720.2 0 111 280 140 720.2 0 140 281 110 720.2 0 110 282 657 720.2 0 657 283 1764 720.2 0 1764 284 956 720.2 0 956 285 72 720.2 0 72 286 60 720.2 0 60 287 91 720.2 0 91 288 105 720.2 0 105 289 87 720.2 0 87 290 84 720.2 0 84 291 118 720.2 0 118 292 257 720.2 0 257 293 268 720.2 0 268 294 376 720.2 0 376 295 131 720.2 0 131 296 119 720.2 0 119 297 111 720.2 0 111 298 138 720.2 0 138 299 116 720.2 0 116 300 172 720.2 0 172 301 380 720.2 0 380 Finished in 17.04 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 265,638 Reads with adapters: 59,909 (22.6%) Reads that were too short: 1,048 (0.4%) Reads written (passing filters): 58,861 (22.2%) Total basepairs processed: 58,607,180 bp Total written (filtered): 16,250,977 bp (27.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 59909 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 30 0.0 3 0 0 19 11 16 438 0.0 3 0 361 51 26 17 57605 0.0 3 54624 2504 394 83 18 138 0.0 3 24 100 10 4 19 153 0.0 3 13 2 74 64 20 130 0.0 3 0 2 0 128 21 2 0.0 3 1 0 0 1 23 5 0.0 3 0 0 0 5 24 3 0.0 3 2 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 1 30 1 0.0 3 1 32 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 1 0.0 3 1 40 2 0.0 3 0 0 0 2 42 3 0.0 3 0 0 0 3 43 6 0.0 3 0 0 0 6 44 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 8 0.0 3 3 3 0 2 53 4 0.0 3 0 0 0 4 59 1 0.0 3 0 0 0 1 62 7 0.0 3 0 0 0 7 63 6 0.0 3 1 0 0 5 64 7 0.0 3 0 0 1 6 65 7 0.0 3 0 0 0 7 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 6 0.0 3 0 0 0 6 83 5 0.0 3 0 0 0 5 86 44 0.0 3 0 0 0 44 87 5 0.0 3 0 0 0 5 88 9 0.0 3 0 0 0 9 89 20 0.0 3 0 0 2 18 90 1 0.0 3 0 0 0 1 91 4 0.0 3 0 0 0 4 92 22 0.0 3 0 0 0 22 93 3 0.0 3 0 0 0 3 94 6 0.0 3 0 0 0 6 95 7 0.0 3 0 0 0 7 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 4 0.0 3 0 0 0 4 106 28 0.0 3 0 0 0 28 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 9 0.0 3 0 0 0 9 110 7 0.0 3 0 0 0 7 111 84 0.0 3 0 0 2 82 112 5 0.0 3 0 0 0 5 113 2 0.0 3 0 0 0 2 114 7 0.0 3 0 0 0 7 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 11 0.0 3 0 0 0 11 125 1 0.0 3 0 0 0 1 126 9 0.0 3 0 0 0 9 127 1 0.0 3 0 0 0 1 128 5 0.0 3 0 0 0 5 129 12 0.0 3 0 0 1 11 130 8 0.0 3 0 0 0 8 132 6 0.0 3 0 0 0 6 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 137 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 140 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 151 799 0.0 3 0 0 19 780 152 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 166 9 0.0 3 0 0 1 8 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 0 3 171 6 0.0 3 0 0 0 6 173 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 1 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 4 0.0 3 1 0 0 3 191 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 9 0.0 3 0 0 0 9 196 3 0.0 3 0 0 0 3 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 3 0.0 3 0 0 1 2 202 4 0.0 3 0 0 0 4 203 2 0.0 3 0 0 0 2 204 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 210 5 0.0 3 0 0 1 4 211 5 0.0 3 0 0 0 5 212 3 0.0 3 0 0 0 3 213 10 0.0 3 0 0 0 10 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 1 231 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.30 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 58,861 Reads with adapters: 109 (0.2%) Reads written (passing filters): 58,861 (100.0%) Total basepairs processed: 16,250,977 bp Total written (filtered): 16,250,609 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 109 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.2% C: 34.9% G: 26.6% T: 18.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 90 919.7 0 90 4 4 229.9 0 4 5 14 57.5 0 14 12 1 0.0 1 0 1