This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.72 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 152,779 (5.2%) Reads that were too short: 91,408 (3.1%) Reads written (passing filters): 61,371 (2.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 10,859,006 bp (1.2%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 152779 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 720.2 0 129 7 4065 720.2 0 4065 8 89 720.2 0 89 9 83 720.2 0 83 10 87 720.2 0 87 11 93 720.2 0 93 12 81 720.2 0 81 13 121 720.2 0 121 14 91 720.2 0 91 15 74 720.2 0 74 16 68 720.2 0 68 17 63 720.2 0 63 18 62 720.2 0 62 19 84 720.2 0 84 20 70 720.2 0 70 21 64 720.2 0 64 22 68 720.2 0 68 23 86 720.2 0 86 24 72 720.2 0 72 25 80 720.2 0 80 26 91 720.2 0 91 27 76 720.2 0 76 28 75 720.2 0 75 29 60 720.2 0 60 30 77 720.2 0 77 31 70 720.2 0 70 32 66 720.2 0 66 33 74 720.2 0 74 34 83 720.2 0 83 35 76 720.2 0 76 36 59 720.2 0 59 37 65 720.2 0 65 38 74 720.2 0 74 39 74 720.2 0 74 40 76 720.2 0 76 41 67 720.2 0 67 42 73 720.2 0 73 43 78 720.2 0 78 44 114 720.2 0 114 45 85 720.2 0 85 46 82 720.2 0 82 47 72 720.2 0 72 48 64 720.2 0 64 49 54 720.2 0 54 50 67 720.2 0 67 51 60 720.2 0 60 52 70 720.2 0 70 53 55 720.2 0 55 54 86 720.2 0 86 55 72 720.2 0 72 56 88 720.2 0 88 57 96 720.2 0 96 58 80 720.2 0 80 59 83 720.2 0 83 60 74 720.2 0 74 61 64 720.2 0 64 62 69 720.2 0 69 63 86 720.2 0 86 64 82 720.2 0 82 65 65 720.2 0 65 66 82 720.2 0 82 67 75 720.2 0 75 68 81 720.2 0 81 69 96 720.2 0 96 70 85 720.2 0 85 71 78 720.2 0 78 72 90 720.2 0 90 73 109 720.2 0 109 74 210 720.2 0 210 75 107 720.2 0 107 76 826 720.2 0 826 77 271 720.2 0 271 78 621 720.2 0 621 79 87 720.2 0 87 80 71 720.2 0 71 81 73 720.2 0 73 82 81 720.2 0 81 83 81 720.2 0 81 84 61 720.2 0 61 85 76 720.2 0 76 86 51 720.2 0 51 87 69 720.2 0 69 88 70 720.2 0 70 89 60 720.2 0 60 90 45 720.2 0 45 91 84 720.2 0 84 92 62 720.2 0 62 93 76 720.2 0 76 94 73 720.2 0 73 95 63 720.2 0 63 96 77 720.2 0 77 97 75 720.2 0 75 98 90 720.2 0 90 99 87 720.2 0 87 100 87 720.2 0 87 101 87 720.2 0 87 102 78 720.2 0 78 103 90 720.2 0 90 104 104 720.2 0 104 105 95 720.2 0 95 106 108 720.2 0 108 107 305 720.2 0 305 108 197 720.2 0 197 109 393 720.2 0 393 110 116 720.2 0 116 111 97 720.2 0 97 112 83 720.2 0 83 113 122 720.2 0 122 114 96 720.2 0 96 115 117 720.2 0 117 116 99 720.2 0 99 117 109 720.2 0 109 118 100 720.2 0 100 119 139 720.2 0 139 120 213 720.2 0 213 121 241 720.2 0 241 122 111 720.2 0 111 123 235 720.2 0 235 124 356 720.2 0 356 125 331 720.2 0 331 126 156 720.2 0 156 127 161 720.2 0 161 128 168 720.2 0 168 129 231 720.2 0 231 130 214 720.2 0 214 131 206 720.2 0 206 132 275 720.2 0 275 133 940 720.2 0 940 134 5533 720.2 0 5533 135 6122 720.2 0 6122 136 4290 720.2 0 4290 137 212 720.2 0 212 138 122 720.2 0 122 139 220 720.2 0 220 140 166 720.2 0 166 141 231 720.2 0 231 142 195 720.2 0 195 143 114 720.2 0 114 144 111 720.2 0 111 145 183 720.2 0 183 146 150 720.2 0 150 147 670 720.2 0 670 148 272 720.2 0 272 149 468 720.2 0 468 150 121 720.2 0 121 151 141 720.2 0 141 152 165 720.2 0 165 153 196 720.2 0 196 154 1224 720.2 0 1224 155 2432 720.2 0 2432 156 1153 720.2 0 1153 157 6135 720.2 0 6135 158 1905 720.2 0 1905 159 4538 720.2 0 4538 160 209 720.2 0 209 161 260 720.2 0 260 162 221 720.2 0 221 163 120 720.2 0 120 164 248 720.2 0 248 165 171 720.2 0 171 166 338 720.2 0 338 167 182 720.2 0 182 168 96 720.2 0 96 169 90 720.2 0 90 170 63 720.2 0 63 171 73 720.2 0 73 172 95 720.2 0 95 173 109 720.2 0 109 174 114 720.2 0 114 175 100 720.2 0 100 176 100 720.2 0 100 177 105 720.2 0 105 178 84 720.2 0 84 179 69 720.2 0 69 180 94 720.2 0 94 181 99 720.2 0 99 182 90 720.2 0 90 183 98 720.2 0 98 184 127 720.2 0 127 185 102 720.2 0 102 186 158 720.2 0 158 187 92 720.2 0 92 188 115 720.2 0 115 189 110 720.2 0 110 190 91 720.2 0 91 191 126 720.2 0 126 192 100 720.2 0 100 193 101 720.2 0 101 194 81 720.2 0 81 195 58 720.2 0 58 196 94 720.2 0 94 197 72 720.2 0 72 198 58 720.2 0 58 199 71 720.2 0 71 200 111 720.2 0 111 201 92 720.2 0 92 202 106 720.2 0 106 203 95 720.2 0 95 204 98 720.2 0 98 205 105 720.2 0 105 206 118 720.2 0 118 207 127 720.2 0 127 208 114 720.2 0 114 209 85 720.2 0 85 210 84 720.2 0 84 211 93 720.2 0 93 212 79 720.2 0 79 213 137 720.2 0 137 214 143 720.2 0 143 215 171 720.2 0 171 216 232 720.2 0 232 217 100 720.2 0 100 218 135 720.2 0 135 219 111 720.2 0 111 220 124 720.2 0 124 221 126 720.2 0 126 222 109 720.2 0 109 223 106 720.2 0 106 224 80 720.2 0 80 225 164 720.2 0 164 226 136 720.2 0 136 227 141 720.2 0 141 228 128 720.2 0 128 229 126 720.2 0 126 230 105 720.2 0 105 231 110 720.2 0 110 232 145 720.2 0 145 233 157 720.2 0 157 234 159 720.2 0 159 235 97 720.2 0 97 236 81 720.2 0 81 237 76 720.2 0 76 238 108 720.2 0 108 239 102 720.2 0 102 240 89 720.2 0 89 241 102 720.2 0 102 242 103 720.2 0 103 243 135 720.2 0 135 244 98 720.2 0 98 245 98 720.2 0 98 246 83 720.2 0 83 247 89 720.2 0 89 248 71 720.2 0 71 249 85 720.2 0 85 250 103 720.2 0 103 251 147 720.2 0 147 252 339 720.2 0 339 253 271 720.2 0 271 254 365 720.2 0 365 255 140 720.2 0 140 256 88 720.2 0 88 257 126 720.2 0 126 258 93 720.2 0 93 259 116 720.2 0 116 260 116 720.2 0 116 261 111 720.2 0 111 262 130 720.2 0 130 263 128 720.2 0 128 264 109 720.2 0 109 265 91 720.2 0 91 266 136 720.2 0 136 267 103 720.2 0 103 268 266 720.2 0 266 269 280 720.2 0 280 270 679 720.2 0 679 271 726 720.2 0 726 272 472 720.2 0 472 273 539 720.2 0 539 274 340 720.2 0 340 275 351 720.2 0 351 276 883 720.2 0 883 277 1123 720.2 0 1123 278 1732 720.2 0 1732 279 1086 720.2 0 1086 280 236 720.2 0 236 281 295 720.2 0 295 282 492 720.2 0 492 283 244 720.2 0 244 284 254 720.2 0 254 285 356 720.2 0 356 286 198 720.2 0 198 287 188 720.2 0 188 288 311 720.2 0 311 289 448 720.2 0 448 290 787 720.2 0 787 291 608 720.2 0 608 292 532 720.2 0 532 293 706 720.2 0 706 294 1091 720.2 0 1091 295 16622 720.2 0 16622 296 33609 720.2 0 33609 297 17265 720.2 0 17265 298 165 720.2 0 165 299 107 720.2 0 107 300 99 720.2 0 99 301 140 720.2 0 140 Finished in 3.45 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 61,371 Reads with adapters: 2,578 (4.2%) Reads that were too short: 87 (0.1%) Reads written (passing filters): 2,491 (4.1%) Total basepairs processed: 10,859,006 bp Total written (filtered): 645,779 bp (5.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2578 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 2 16 15 0.0 3 0 10 2 3 17 2186 0.0 3 1776 361 45 4 18 4 0.0 3 0 1 1 2 19 7 0.0 3 3 1 2 1 20 4 0.0 3 0 0 0 4 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 27 6 0.0 3 0 0 0 6 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 52 0.0 3 0 0 1 51 33 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 35 13 0.0 3 0 0 0 13 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 5 0.0 3 0 0 0 5 56 2 0.0 3 0 0 0 2 58 138 0.0 3 0 0 0 138 59 2 0.0 3 0 0 0 2 61 5 0.0 3 0 0 1 4 65 1 0.0 3 0 0 0 1 68 23 0.0 3 0 0 1 22 70 2 0.0 3 0 0 1 1 71 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 5 0.0 3 0 0 0 5 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 30 0.0 3 0 0 0 30 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 1 118 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 152 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 1 169 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 2,491 Reads with adapters: 7 (0.3%) Reads written (passing filters): 2,491 (100.0%) Total basepairs processed: 645,779 bp Total written (filtered): 645,756 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 7 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 57.1% G: 42.9% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 38.9 0 5 4 2 9.7 0 2