This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 142.01 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 181,697 (6.2%) Reads that were too short: 116,277 (3.9%) Reads written (passing filters): 65,420 (2.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 11,629,586 bp (1.3%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 181697 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 144 720.2 0 144 7 4147 720.2 0 4147 8 119 720.2 0 119 9 92 720.2 0 92 10 118 720.2 0 118 11 110 720.2 0 110 12 82 720.2 0 82 13 126 720.2 0 126 14 121 720.2 0 121 15 95 720.2 0 95 16 109 720.2 0 109 17 98 720.2 0 98 18 97 720.2 0 97 19 105 720.2 0 105 20 99 720.2 0 99 21 100 720.2 0 100 22 88 720.2 0 88 23 105 720.2 0 105 24 101 720.2 0 101 25 113 720.2 0 113 26 93 720.2 0 93 27 80 720.2 0 80 28 97 720.2 0 97 29 78 720.2 0 78 30 94 720.2 0 94 31 98 720.2 0 98 32 100 720.2 0 100 33 81 720.2 0 81 34 99 720.2 0 99 35 94 720.2 0 94 36 81 720.2 0 81 37 89 720.2 0 89 38 95 720.2 0 95 39 81 720.2 0 81 40 90 720.2 0 90 41 88 720.2 0 88 42 106 720.2 0 106 43 99 720.2 0 99 44 104 720.2 0 104 45 84 720.2 0 84 46 116 720.2 0 116 47 94 720.2 0 94 48 88 720.2 0 88 49 86 720.2 0 86 50 98 720.2 0 98 51 85 720.2 0 85 52 75 720.2 0 75 53 73 720.2 0 73 54 101 720.2 0 101 55 72 720.2 0 72 56 103 720.2 0 103 57 93 720.2 0 93 58 101 720.2 0 101 59 98 720.2 0 98 60 115 720.2 0 115 61 113 720.2 0 113 62 99 720.2 0 99 63 95 720.2 0 95 64 105 720.2 0 105 65 118 720.2 0 118 66 96 720.2 0 96 67 94 720.2 0 94 68 72 720.2 0 72 69 125 720.2 0 125 70 118 720.2 0 118 71 85 720.2 0 85 72 93 720.2 0 93 73 119 720.2 0 119 74 212 720.2 0 212 75 122 720.2 0 122 76 702 720.2 0 702 77 191 720.2 0 191 78 286 720.2 0 286 79 118 720.2 0 118 80 132 720.2 0 132 81 122 720.2 0 122 82 107 720.2 0 107 83 132 720.2 0 132 84 122 720.2 0 122 85 133 720.2 0 133 86 102 720.2 0 102 87 138 720.2 0 138 88 127 720.2 0 127 89 132 720.2 0 132 90 95 720.2 0 95 91 122 720.2 0 122 92 93 720.2 0 93 93 117 720.2 0 117 94 106 720.2 0 106 95 116 720.2 0 116 96 102 720.2 0 102 97 105 720.2 0 105 98 88 720.2 0 88 99 137 720.2 0 137 100 127 720.2 0 127 101 131 720.2 0 131 102 88 720.2 0 88 103 149 720.2 0 149 104 127 720.2 0 127 105 121 720.2 0 121 106 151 720.2 0 151 107 203 720.2 0 203 108 165 720.2 0 165 109 254 720.2 0 254 110 143 720.2 0 143 111 157 720.2 0 157 112 125 720.2 0 125 113 139 720.2 0 139 114 145 720.2 0 145 115 129 720.2 0 129 116 115 720.2 0 115 117 150 720.2 0 150 118 150 720.2 0 150 119 162 720.2 0 162 120 196 720.2 0 196 121 258 720.2 0 258 122 174 720.2 0 174 123 201 720.2 0 201 124 240 720.2 0 240 125 200 720.2 0 200 126 175 720.2 0 175 127 206 720.2 0 206 128 192 720.2 0 192 129 245 720.2 0 245 130 210 720.2 0 210 131 226 720.2 0 226 132 259 720.2 0 259 133 898 720.2 0 898 134 5513 720.2 0 5513 135 5993 720.2 0 5993 136 4074 720.2 0 4074 137 253 720.2 0 253 138 153 720.2 0 153 139 228 720.2 0 228 140 189 720.2 0 189 141 277 720.2 0 277 142 199 720.2 0 199 143 143 720.2 0 143 144 117 720.2 0 117 145 156 720.2 0 156 146 162 720.2 0 162 147 517 720.2 0 517 148 234 720.2 0 234 149 373 720.2 0 373 150 151 720.2 0 151 151 148 720.2 0 148 152 200 720.2 0 200 153 198 720.2 0 198 154 1257 720.2 0 1257 155 2637 720.2 0 2637 156 1130 720.2 0 1130 157 7527 720.2 0 7527 158 2187 720.2 0 2187 159 5287 720.2 0 5287 160 173 720.2 0 173 161 179 720.2 0 179 162 130 720.2 0 130 163 105 720.2 0 105 164 150 720.2 0 150 165 123 720.2 0 123 166 171 720.2 0 171 167 148 720.2 0 148 168 104 720.2 0 104 169 113 720.2 0 113 170 96 720.2 0 96 171 99 720.2 0 99 172 126 720.2 0 126 173 95 720.2 0 95 174 128 720.2 0 128 175 109 720.2 0 109 176 119 720.2 0 119 177 96 720.2 0 96 178 97 720.2 0 97 179 88 720.2 0 88 180 98 720.2 0 98 181 101 720.2 0 101 182 119 720.2 0 119 183 95 720.2 0 95 184 116 720.2 0 116 185 112 720.2 0 112 186 138 720.2 0 138 187 107 720.2 0 107 188 126 720.2 0 126 189 111 720.2 0 111 190 92 720.2 0 92 191 125 720.2 0 125 192 104 720.2 0 104 193 146 720.2 0 146 194 118 720.2 0 118 195 85 720.2 0 85 196 93 720.2 0 93 197 95 720.2 0 95 198 76 720.2 0 76 199 118 720.2 0 118 200 97 720.2 0 97 201 96 720.2 0 96 202 144 720.2 0 144 203 95 720.2 0 95 204 105 720.2 0 105 205 118 720.2 0 118 206 120 720.2 0 120 207 126 720.2 0 126 208 116 720.2 0 116 209 95 720.2 0 95 210 86 720.2 0 86 211 108 720.2 0 108 212 122 720.2 0 122 213 123 720.2 0 123 214 129 720.2 0 129 215 110 720.2 0 110 216 273 720.2 0 273 217 113 720.2 0 113 218 97 720.2 0 97 219 86 720.2 0 86 220 103 720.2 0 103 221 124 720.2 0 124 222 110 720.2 0 110 223 110 720.2 0 110 224 97 720.2 0 97 225 277 720.2 0 277 226 129 720.2 0 129 227 145 720.2 0 145 228 138 720.2 0 138 229 117 720.2 0 117 230 104 720.2 0 104 231 111 720.2 0 111 232 114 720.2 0 114 233 108 720.2 0 108 234 143 720.2 0 143 235 105 720.2 0 105 236 102 720.2 0 102 237 81 720.2 0 81 238 119 720.2 0 119 239 101 720.2 0 101 240 94 720.2 0 94 241 106 720.2 0 106 242 98 720.2 0 98 243 89 720.2 0 89 244 97 720.2 0 97 245 99 720.2 0 99 246 93 720.2 0 93 247 94 720.2 0 94 248 103 720.2 0 103 249 87 720.2 0 87 250 114 720.2 0 114 251 173 720.2 0 173 252 259 720.2 0 259 253 224 720.2 0 224 254 299 720.2 0 299 255 108 720.2 0 108 256 107 720.2 0 107 257 111 720.2 0 111 258 80 720.2 0 80 259 101 720.2 0 101 260 107 720.2 0 107 261 121 720.2 0 121 262 128 720.2 0 128 263 100 720.2 0 100 264 114 720.2 0 114 265 110 720.2 0 110 266 117 720.2 0 117 267 110 720.2 0 110 268 171 720.2 0 171 269 183 720.2 0 183 270 543 720.2 0 543 271 707 720.2 0 707 272 479 720.2 0 479 273 504 720.2 0 504 274 301 720.2 0 301 275 316 720.2 0 316 276 1005 720.2 0 1005 277 962 720.2 0 962 278 1777 720.2 0 1777 279 1165 720.2 0 1165 280 239 720.2 0 239 281 341 720.2 0 341 282 628 720.2 0 628 283 285 720.2 0 285 284 330 720.2 0 330 285 440 720.2 0 440 286 216 720.2 0 216 287 203 720.2 0 203 288 421 720.2 0 421 289 498 720.2 0 498 290 773 720.2 0 773 291 703 720.2 0 703 292 596 720.2 0 596 293 780 720.2 0 780 294 1114 720.2 0 1114 295 21469 720.2 0 21469 296 46686 720.2 0 46686 297 23962 720.2 0 23962 298 162 720.2 0 162 299 78 720.2 0 78 300 95 720.2 0 95 301 98 720.2 0 98 Finished in 4.02 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 65,420 Reads with adapters: 2,419 (3.7%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 2,406 (3.7%) Total basepairs processed: 11,629,586 bp Total written (filtered): 649,525 bp (5.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2419 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 17 0.0 3 0 16 1 20 2296 0.0 4 2077 211 8 21 1 0.0 4 1 22 1 0.0 4 0 0 0 0 1 81 3 0.0 4 0 0 0 0 3 82 4 0.0 4 0 0 0 0 4 83 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 89 0.0 4 0 0 0 0 89 132 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 1 217 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 2,406 Reads with adapters: 69 (2.9%) Reads written (passing filters): 2,406 (100.0%) Total basepairs processed: 649,525 bp Total written (filtered): 648,827 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 69 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 5.8% G: 88.4% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 37.6 0 4 4 1 9.4 0 1 5 3 2.3 0 3 6 1 0.6 0 1 10 1 0.0 1 0 1 11 57 0.0 1 0 57 12 2 0.0 1 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.