This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.55 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 160,455 (5.4%) Reads that were too short: 18,876 (0.6%) Reads written (passing filters): 141,579 (4.8%) Total basepairs processed: 887,907,860 bp Total written (filtered): 37,287,408 bp (4.2%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 160455 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1301 720.2 0 1301 7 1594 720.2 0 1594 8 57313 720.2 0 57313 9 43697 720.2 0 43697 10 41 720.2 0 41 11 5 720.2 0 5 12 5 720.2 0 5 13 11 720.2 0 11 14 101 720.2 0 101 15 140 720.2 0 140 16 21 720.2 0 21 17 7 720.2 0 7 18 59 720.2 0 59 19 40 720.2 0 40 20 49 720.2 0 49 21 16 720.2 0 16 22 3 720.2 0 3 23 5 720.2 0 5 24 1 720.2 0 1 25 5 720.2 0 5 26 19 720.2 0 19 27 29 720.2 0 29 28 11 720.2 0 11 29 5 720.2 0 5 30 6 720.2 0 6 31 7 720.2 0 7 32 9 720.2 0 9 33 7 720.2 0 7 34 2 720.2 0 2 35 4 720.2 0 4 36 5 720.2 0 5 38 4 720.2 0 4 39 11 720.2 0 11 40 14 720.2 0 14 41 21 720.2 0 21 42 10 720.2 0 10 43 50 720.2 0 50 44 175 720.2 0 175 45 299 720.2 0 299 46 136 720.2 0 136 47 10 720.2 0 10 48 9 720.2 0 9 49 9 720.2 0 9 50 35 720.2 0 35 51 17 720.2 0 17 52 14 720.2 0 14 53 13 720.2 0 13 54 8 720.2 0 8 55 42 720.2 0 42 56 13 720.2 0 13 57 9 720.2 0 9 58 13 720.2 0 13 59 28 720.2 0 28 60 40 720.2 0 40 61 15 720.2 0 15 62 19 720.2 0 19 63 20 720.2 0 20 64 26 720.2 0 26 65 28 720.2 0 28 66 25 720.2 0 25 67 57 720.2 0 57 68 42 720.2 0 42 69 18 720.2 0 18 70 27 720.2 0 27 71 45 720.2 0 45 72 66 720.2 0 66 73 77 720.2 0 77 74 130 720.2 0 130 75 205 720.2 0 205 76 255 720.2 0 255 77 2516 720.2 0 2516 78 10040 720.2 0 10040 79 5075 720.2 0 5075 80 22 720.2 0 22 81 45 720.2 0 45 82 19 720.2 0 19 83 8 720.2 0 8 84 11 720.2 0 11 85 31 720.2 0 31 86 23 720.2 0 23 87 6 720.2 0 6 88 3 720.2 0 3 89 5 720.2 0 5 90 9 720.2 0 9 91 33 720.2 0 33 92 59 720.2 0 59 93 67 720.2 0 67 94 29 720.2 0 29 95 17 720.2 0 17 96 47 720.2 0 47 97 4 720.2 0 4 98 6 720.2 0 6 99 20 720.2 0 20 100 40 720.2 0 40 101 74 720.2 0 74 102 1157 720.2 0 1157 103 40 720.2 0 40 104 9 720.2 0 9 105 12 720.2 0 12 106 5 720.2 0 5 107 5 720.2 0 5 108 11 720.2 0 11 109 6 720.2 0 6 110 3 720.2 0 3 111 6 720.2 0 6 112 12 720.2 0 12 113 7 720.2 0 7 114 32 720.2 0 32 115 72 720.2 0 72 116 118 720.2 0 118 117 24 720.2 0 24 118 12 720.2 0 12 119 13 720.2 0 13 120 32 720.2 0 32 121 20 720.2 0 20 122 51 720.2 0 51 123 33 720.2 0 33 124 9 720.2 0 9 125 9 720.2 0 9 126 21 720.2 0 21 127 18 720.2 0 18 128 18 720.2 0 18 129 58 720.2 0 58 130 21 720.2 0 21 131 17 720.2 0 17 132 16 720.2 0 16 133 13 720.2 0 13 134 39 720.2 0 39 135 74 720.2 0 74 136 182 720.2 0 182 137 491 720.2 0 491 138 132 720.2 0 132 139 61 720.2 0 61 140 54 720.2 0 54 141 28 720.2 0 28 142 21 720.2 0 21 143 64 720.2 0 64 144 51 720.2 0 51 145 14 720.2 0 14 146 45 720.2 0 45 147 11 720.2 0 11 148 13 720.2 0 13 149 23 720.2 0 23 150 40 720.2 0 40 151 113 720.2 0 113 152 26 720.2 0 26 153 14 720.2 0 14 154 12 720.2 0 12 155 13 720.2 0 13 156 18 720.2 0 18 157 32 720.2 0 32 158 121 720.2 0 121 159 25 720.2 0 25 160 34 720.2 0 34 161 27 720.2 0 27 162 75 720.2 0 75 163 93 720.2 0 93 164 71 720.2 0 71 165 79 720.2 0 79 166 95 720.2 0 95 167 17 720.2 0 17 168 29 720.2 0 29 169 26 720.2 0 26 170 43 720.2 0 43 171 44 720.2 0 44 172 79 720.2 0 79 173 735 720.2 0 735 174 1187 720.2 0 1187 175 903 720.2 0 903 176 73 720.2 0 73 177 21 720.2 0 21 178 46 720.2 0 46 179 63 720.2 0 63 180 699 720.2 0 699 181 332 720.2 0 332 182 995 720.2 0 995 183 60 720.2 0 60 184 43 720.2 0 43 185 59 720.2 0 59 186 57 720.2 0 57 187 54 720.2 0 54 188 56 720.2 0 56 189 108 720.2 0 108 190 179 720.2 0 179 191 1956 720.2 0 1956 192 1093 720.2 0 1093 193 3401 720.2 0 3401 194 206 720.2 0 206 195 20 720.2 0 20 196 18 720.2 0 18 197 16 720.2 0 16 198 29 720.2 0 29 199 42 720.2 0 42 200 29 720.2 0 29 201 28 720.2 0 28 202 330 720.2 0 330 203 269 720.2 0 269 204 580 720.2 0 580 205 215 720.2 0 215 206 199 720.2 0 199 207 80 720.2 0 80 208 85 720.2 0 85 209 204 720.2 0 204 210 143 720.2 0 143 211 86 720.2 0 86 212 18 720.2 0 18 213 11 720.2 0 11 214 10 720.2 0 10 215 25 720.2 0 25 216 38 720.2 0 38 217 62 720.2 0 62 218 48 720.2 0 48 219 30 720.2 0 30 220 17 720.2 0 17 221 15 720.2 0 15 222 6 720.2 0 6 223 10 720.2 0 10 224 10 720.2 0 10 225 19 720.2 0 19 226 25 720.2 0 25 227 29 720.2 0 29 228 34 720.2 0 34 229 118 720.2 0 118 230 21 720.2 0 21 231 14 720.2 0 14 232 15 720.2 0 15 233 33 720.2 0 33 234 39 720.2 0 39 235 37 720.2 0 37 236 37 720.2 0 37 237 28 720.2 0 28 238 20 720.2 0 20 239 20 720.2 0 20 240 17 720.2 0 17 241 15 720.2 0 15 242 22 720.2 0 22 243 28 720.2 0 28 244 21 720.2 0 21 245 24 720.2 0 24 246 27 720.2 0 27 247 28 720.2 0 28 248 24 720.2 0 24 249 34 720.2 0 34 250 24 720.2 0 24 251 66 720.2 0 66 252 202 720.2 0 202 253 391 720.2 0 391 254 473 720.2 0 473 255 108 720.2 0 108 256 29 720.2 0 29 257 36 720.2 0 36 258 93 720.2 0 93 259 268 720.2 0 268 260 722 720.2 0 722 261 1497 720.2 0 1497 262 612 720.2 0 612 263 139 720.2 0 139 264 141 720.2 0 141 265 126 720.2 0 126 266 198 720.2 0 198 267 42 720.2 0 42 268 31 720.2 0 31 269 22 720.2 0 22 270 53 720.2 0 53 271 104 720.2 0 104 272 93 720.2 0 93 273 107 720.2 0 107 274 71 720.2 0 71 275 32 720.2 0 32 276 35 720.2 0 35 277 69 720.2 0 69 278 78 720.2 0 78 279 124 720.2 0 124 280 116 720.2 0 116 281 104 720.2 0 104 282 66 720.2 0 66 283 194 720.2 0 194 284 1865 720.2 0 1865 285 3257 720.2 0 3257 286 2303 720.2 0 2303 287 282 720.2 0 282 288 59 720.2 0 59 289 36 720.2 0 36 290 48 720.2 0 48 291 71 720.2 0 71 292 41 720.2 0 41 293 136 720.2 0 136 294 40 720.2 0 40 295 231 720.2 0 231 296 350 720.2 0 350 297 207 720.2 0 207 298 114 720.2 0 114 299 35 720.2 0 35 300 26 720.2 0 26 301 89 720.2 0 89 Finished in 10.28 s (73 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 141,579 Reads with adapters: 56,480 (39.9%) Reads that were too short: 103 (0.1%) Reads written (passing filters): 56,377 (39.8%) Total basepairs processed: 37,287,408 bp Total written (filtered): 15,529,381 bp (41.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 56480 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 26 0.0 3 0 0 21 5 16 447 0.0 3 0 387 38 22 17 54766 0.0 3 51864 2461 365 76 18 276 0.0 3 22 104 19 131 19 140 0.0 3 0 11 83 46 20 618 0.0 3 510 24 2 82 21 14 0.0 3 1 1 0 12 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 1 2 24 1 0.0 3 0 0 1 25 1 0.0 3 0 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 16 0.0 3 0 0 0 16 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 36 1 0.0 3 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 1 1 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 59 2 0.0 3 1 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 1 68 3 0.0 3 0 0 0 3 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 5 0.0 3 0 0 0 5 80 1 0.0 3 0 0 0 1 81 10 0.0 3 5 2 1 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 1 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 3 0.0 3 0 0 0 3 113 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 119 5 0.0 3 0 0 0 5 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 1 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 1 147 3 0.0 3 0 0 0 3 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 158 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 4 0.0 3 0 0 0 4 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 194 6 0.0 3 0 0 0 6 195 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.30 s (59 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 56,377 Reads with adapters: 379 (0.7%) Reads written (passing filters): 56,377 (100.0%) Total basepairs processed: 15,529,381 bp Total written (filtered): 15,527,980 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 379 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.2% C: 3.7% G: 68.3% T: 4.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 128 880.9 0 128 4 243 220.2 0 243 5 7 55.1 0 7 10 1 0.1 1 0 1