This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.92 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 152,207 (5.2%) Reads that were too short: 9,692 (0.3%) Reads written (passing filters): 142,515 (4.8%) Total basepairs processed: 887,907,860 bp Total written (filtered): 37,886,251 bp (4.3%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 152207 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1153 720.2 0 1153 7 1371 720.2 0 1371 8 50729 720.2 0 50729 9 53832 720.2 0 53832 10 41 720.2 0 41 11 5 720.2 0 5 12 3 720.2 0 3 13 5 720.2 0 5 14 2 720.2 0 2 15 1 720.2 0 1 16 4 720.2 0 4 18 14 720.2 0 14 19 10 720.2 0 10 20 24 720.2 0 24 21 3 720.2 0 3 22 1 720.2 0 1 23 2 720.2 0 2 24 1 720.2 0 1 25 2 720.2 0 2 27 4 720.2 0 4 28 5 720.2 0 5 29 3 720.2 0 3 30 6 720.2 0 6 31 6 720.2 0 6 32 8 720.2 0 8 33 7 720.2 0 7 34 2 720.2 0 2 35 4 720.2 0 4 36 7 720.2 0 7 37 7 720.2 0 7 38 7 720.2 0 7 39 18 720.2 0 18 40 11 720.2 0 11 41 5 720.2 0 5 42 12 720.2 0 12 43 22 720.2 0 22 44 43 720.2 0 43 45 44 720.2 0 44 46 41 720.2 0 41 47 12 720.2 0 12 48 11 720.2 0 11 49 11 720.2 0 11 50 35 720.2 0 35 51 11 720.2 0 11 52 10 720.2 0 10 53 7 720.2 0 7 54 10 720.2 0 10 55 37 720.2 0 37 56 16 720.2 0 16 57 15 720.2 0 15 58 14 720.2 0 14 59 33 720.2 0 33 60 24 720.2 0 24 61 14 720.2 0 14 62 16 720.2 0 16 63 10 720.2 0 10 64 25 720.2 0 25 65 42 720.2 0 42 66 15 720.2 0 15 67 24 720.2 0 24 68 27 720.2 0 27 69 13 720.2 0 13 70 17 720.2 0 17 71 23 720.2 0 23 72 54 720.2 0 54 73 104 720.2 0 104 74 149 720.2 0 149 75 218 720.2 0 218 76 234 720.2 0 234 77 2604 720.2 0 2604 78 10062 720.2 0 10062 79 6189 720.2 0 6189 80 37 720.2 0 37 81 49 720.2 0 49 82 9 720.2 0 9 83 3 720.2 0 3 84 9 720.2 0 9 85 5 720.2 0 5 86 5 720.2 0 5 87 6 720.2 0 6 88 4 720.2 0 4 89 8 720.2 0 8 90 7 720.2 0 7 91 35 720.2 0 35 92 61 720.2 0 61 93 85 720.2 0 85 94 37 720.2 0 37 95 17 720.2 0 17 96 47 720.2 0 47 97 7 720.2 0 7 98 8 720.2 0 8 99 25 720.2 0 25 100 58 720.2 0 58 101 32 720.2 0 32 102 1422 720.2 0 1422 103 59 720.2 0 59 104 20 720.2 0 20 105 7 720.2 0 7 106 7 720.2 0 7 107 7 720.2 0 7 108 10 720.2 0 10 109 5 720.2 0 5 110 2 720.2 0 2 112 5 720.2 0 5 113 6 720.2 0 6 114 13 720.2 0 13 115 37 720.2 0 37 116 115 720.2 0 115 117 12 720.2 0 12 118 18 720.2 0 18 119 10 720.2 0 10 120 23 720.2 0 23 121 12 720.2 0 12 122 31 720.2 0 31 123 18 720.2 0 18 124 5 720.2 0 5 125 9 720.2 0 9 126 10 720.2 0 10 127 7 720.2 0 7 128 13 720.2 0 13 129 32 720.2 0 32 130 3 720.2 0 3 131 21 720.2 0 21 132 6 720.2 0 6 133 14 720.2 0 14 134 23 720.2 0 23 135 32 720.2 0 32 136 164 720.2 0 164 137 479 720.2 0 479 138 119 720.2 0 119 139 56 720.2 0 56 140 29 720.2 0 29 141 18 720.2 0 18 142 16 720.2 0 16 143 44 720.2 0 44 144 28 720.2 0 28 145 15 720.2 0 15 146 44 720.2 0 44 147 7 720.2 0 7 148 14 720.2 0 14 149 10 720.2 0 10 150 24 720.2 0 24 151 106 720.2 0 106 152 22 720.2 0 22 153 13 720.2 0 13 154 11 720.2 0 11 155 11 720.2 0 11 156 10 720.2 0 10 157 26 720.2 0 26 158 81 720.2 0 81 159 8 720.2 0 8 160 18 720.2 0 18 161 11 720.2 0 11 162 51 720.2 0 51 163 42 720.2 0 42 164 37 720.2 0 37 165 33 720.2 0 33 166 29 720.2 0 29 167 18 720.2 0 18 168 16 720.2 0 16 169 13 720.2 0 13 170 13 720.2 0 13 171 11 720.2 0 11 172 25 720.2 0 25 173 305 720.2 0 305 174 603 720.2 0 603 175 427 720.2 0 427 176 63 720.2 0 63 177 26 720.2 0 26 178 31 720.2 0 31 179 56 720.2 0 56 180 438 720.2 0 438 181 201 720.2 0 201 182 528 720.2 0 528 183 21 720.2 0 21 184 11 720.2 0 11 185 23 720.2 0 23 186 37 720.2 0 37 187 41 720.2 0 41 188 43 720.2 0 43 189 104 720.2 0 104 190 130 720.2 0 130 191 2317 720.2 0 2317 192 1102 720.2 0 1102 193 3926 720.2 0 3926 194 189 720.2 0 189 195 10 720.2 0 10 196 13 720.2 0 13 197 12 720.2 0 12 198 18 720.2 0 18 199 15 720.2 0 15 200 15 720.2 0 15 201 19 720.2 0 19 202 327 720.2 0 327 203 210 720.2 0 210 204 487 720.2 0 487 205 169 720.2 0 169 206 257 720.2 0 257 207 64 720.2 0 64 208 26 720.2 0 26 209 43 720.2 0 43 210 35 720.2 0 35 211 27 720.2 0 27 212 7 720.2 0 7 213 12 720.2 0 12 214 5 720.2 0 5 215 10 720.2 0 10 216 13 720.2 0 13 217 19 720.2 0 19 218 13 720.2 0 13 219 7 720.2 0 7 220 5 720.2 0 5 221 5 720.2 0 5 222 10 720.2 0 10 223 8 720.2 0 8 224 6 720.2 0 6 225 3 720.2 0 3 226 13 720.2 0 13 227 6 720.2 0 6 228 15 720.2 0 15 229 177 720.2 0 177 230 23 720.2 0 23 231 13 720.2 0 13 232 6 720.2 0 6 233 5 720.2 0 5 234 20 720.2 0 20 235 14 720.2 0 14 236 16 720.2 0 16 237 24 720.2 0 24 238 9 720.2 0 9 239 9 720.2 0 9 240 14 720.2 0 14 241 13 720.2 0 13 242 12 720.2 0 12 243 6 720.2 0 6 244 12 720.2 0 12 245 18 720.2 0 18 246 4 720.2 0 4 247 20 720.2 0 20 248 11 720.2 0 11 249 6 720.2 0 6 250 15 720.2 0 15 251 48 720.2 0 48 252 77 720.2 0 77 253 172 720.2 0 172 254 149 720.2 0 149 255 35 720.2 0 35 256 15 720.2 0 15 257 13 720.2 0 13 258 46 720.2 0 46 259 191 720.2 0 191 260 367 720.2 0 367 261 831 720.2 0 831 262 312 720.2 0 312 263 56 720.2 0 56 264 77 720.2 0 77 265 52 720.2 0 52 266 133 720.2 0 133 267 20 720.2 0 20 268 20 720.2 0 20 269 6 720.2 0 6 270 16 720.2 0 16 271 27 720.2 0 27 272 45 720.2 0 45 273 35 720.2 0 35 274 32 720.2 0 32 275 21 720.2 0 21 276 21 720.2 0 21 277 26 720.2 0 26 278 41 720.2 0 41 279 52 720.2 0 52 280 51 720.2 0 51 281 37 720.2 0 37 282 36 720.2 0 36 283 77 720.2 0 77 284 801 720.2 0 801 285 1350 720.2 0 1350 286 1038 720.2 0 1038 287 362 720.2 0 362 288 45 720.2 0 45 289 25 720.2 0 25 290 23 720.2 0 23 291 42 720.2 0 42 292 22 720.2 0 22 293 124 720.2 0 124 294 9 720.2 0 9 295 159 720.2 0 159 296 168 720.2 0 168 297 60 720.2 0 60 298 27 720.2 0 27 299 12 720.2 0 12 300 7 720.2 0 7 301 32 720.2 0 32 Finished in 11.00 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 142,515 Reads with adapters: 60,653 (42.6%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 60,634 (42.5%) Total basepairs processed: 37,886,251 bp Total written (filtered): 16,182,705 bp (42.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 60653 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 5 0.0 3 0 0 0 1 4 18 26 0.0 3 0 0 25 1 19 906 0.0 3 0 886 13 6 1 20 56525 0.0 4 55561 911 45 7 1 21 147 0.0 4 40 104 3 22 13 0.0 4 4 0 8 0 1 23 36 0.0 4 2 1 2 26 5 25 1 0.0 4 1 28 1 0.0 4 1 45 1 0.0 4 1 47 1 0.0 4 1 59 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 98 1 0.0 4 1 100 1 0.0 4 0 0 0 0 1 125 7 0.0 4 0 0 0 0 7 126 30 0.0 4 0 0 0 0 30 127 2899 0.0 4 0 0 0 0 2899 128 7 0.0 4 0 0 0 0 7 130 21 0.0 4 0 0 0 0 21 131 1 0.0 4 0 0 0 0 1 145 1 0.0 4 1 146 1 0.0 4 1 166 1 0.0 4 0 0 1 171 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.52 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 60,634 Reads with adapters: 7,297 (12.0%) Reads written (passing filters): 60,634 (100.0%) Total basepairs processed: 16,182,705 bp Total written (filtered): 16,108,915 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7297 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.2% G: 95.4% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 947.4 0 7 4 6 236.9 0 6 5 2 59.2 0 2 6 3 14.8 0 3 7 1 3.7 0 1 9 3638 0.2 0 92 3546 10 3103 0.1 1 93 3010 11 505 0.0 1 2 503 12 6 0.0 1 1 5 13 3 0.0 1 0 3 138 1 0.0 1 1 150 6 0.0 1 1 5 151 1 0.0 1 1 152 1 0.0 1 0 1 169 3 0.0 1 2 1 208 2 0.0 1 2 223 8 0.0 1 5 3 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.