This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.73 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 172,358 (5.8%) Reads that were too short: 10,726 (0.4%) Reads written (passing filters): 161,632 (5.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 46,329,747 bp (5.2%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 172358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1259 720.2 0 1259 7 83195 720.2 0 83195 8 66225 720.2 0 66225 9 54 720.2 0 54 10 8 720.2 0 8 11 14 720.2 0 14 12 13 720.2 0 13 13 8 720.2 0 8 14 13 720.2 0 13 15 18 720.2 0 18 16 13 720.2 0 13 17 9 720.2 0 9 18 39 720.2 0 39 19 19 720.2 0 19 20 49 720.2 0 49 21 16 720.2 0 16 22 10 720.2 0 10 23 12 720.2 0 12 24 3 720.2 0 3 25 6 720.2 0 6 26 12 720.2 0 12 27 35 720.2 0 35 28 20 720.2 0 20 29 27 720.2 0 27 30 15 720.2 0 15 31 30 720.2 0 30 32 21 720.2 0 21 33 17 720.2 0 17 34 21 720.2 0 21 35 40 720.2 0 40 36 109 720.2 0 109 37 374 720.2 0 374 38 289 720.2 0 289 39 28 720.2 0 28 40 15 720.2 0 15 41 18 720.2 0 18 42 17 720.2 0 17 43 70 720.2 0 70 44 682 720.2 0 682 45 879 720.2 0 879 46 14 720.2 0 14 47 11 720.2 0 11 48 6 720.2 0 6 49 24 720.2 0 24 50 12 720.2 0 12 51 15 720.2 0 15 52 20 720.2 0 20 53 11 720.2 0 11 54 14 720.2 0 14 55 21 720.2 0 21 56 26 720.2 0 26 57 32 720.2 0 32 58 18 720.2 0 18 59 24 720.2 0 24 60 8 720.2 0 8 61 17 720.2 0 17 62 28 720.2 0 28 63 11 720.2 0 11 64 4 720.2 0 4 65 12 720.2 0 12 66 15 720.2 0 15 67 12 720.2 0 12 68 20 720.2 0 20 69 13 720.2 0 13 70 17 720.2 0 17 71 35 720.2 0 35 72 24 720.2 0 24 73 70 720.2 0 70 74 13 720.2 0 13 75 23 720.2 0 23 76 11 720.2 0 11 77 13 720.2 0 13 78 25 720.2 0 25 79 26 720.2 0 26 80 21 720.2 0 21 81 21 720.2 0 21 82 21 720.2 0 21 83 25 720.2 0 25 84 35 720.2 0 35 85 35 720.2 0 35 86 8 720.2 0 8 87 19 720.2 0 19 88 19 720.2 0 19 89 23 720.2 0 23 90 4 720.2 0 4 91 22 720.2 0 22 92 14 720.2 0 14 93 20 720.2 0 20 94 28 720.2 0 28 95 17 720.2 0 17 96 52 720.2 0 52 97 17 720.2 0 17 98 8 720.2 0 8 99 16 720.2 0 16 100 11 720.2 0 11 101 17 720.2 0 17 102 13 720.2 0 13 103 18 720.2 0 18 104 13 720.2 0 13 105 16 720.2 0 16 106 15 720.2 0 15 107 25 720.2 0 25 108 19 720.2 0 19 109 22 720.2 0 22 110 14 720.2 0 14 111 13 720.2 0 13 112 26 720.2 0 26 113 24 720.2 0 24 114 36 720.2 0 36 115 28 720.2 0 28 116 18 720.2 0 18 117 15 720.2 0 15 118 23 720.2 0 23 119 27 720.2 0 27 120 31 720.2 0 31 121 36 720.2 0 36 122 73 720.2 0 73 123 27 720.2 0 27 124 29 720.2 0 29 125 36 720.2 0 36 126 114 720.2 0 114 127 187 720.2 0 187 128 284 720.2 0 284 129 108 720.2 0 108 130 79 720.2 0 79 131 93 720.2 0 93 132 213 720.2 0 213 133 82 720.2 0 82 134 66 720.2 0 66 135 467 720.2 0 467 136 187 720.2 0 187 137 552 720.2 0 552 138 31 720.2 0 31 139 89 720.2 0 89 140 110 720.2 0 110 141 120 720.2 0 120 142 125 720.2 0 125 143 104 720.2 0 104 144 150 720.2 0 150 145 102 720.2 0 102 146 34 720.2 0 34 147 67 720.2 0 67 148 441 720.2 0 441 149 336 720.2 0 336 150 279 720.2 0 279 151 35 720.2 0 35 152 22 720.2 0 22 153 23 720.2 0 23 154 13 720.2 0 13 155 22 720.2 0 22 156 30 720.2 0 30 157 33 720.2 0 33 158 26 720.2 0 26 159 28 720.2 0 28 160 44 720.2 0 44 161 58 720.2 0 58 162 28 720.2 0 28 163 30 720.2 0 30 164 26 720.2 0 26 165 37 720.2 0 37 166 38 720.2 0 38 167 59 720.2 0 59 168 35 720.2 0 35 169 94 720.2 0 94 170 24 720.2 0 24 171 18 720.2 0 18 172 27 720.2 0 27 173 20 720.2 0 20 174 29 720.2 0 29 175 51 720.2 0 51 176 17 720.2 0 17 177 29 720.2 0 29 178 20 720.2 0 20 179 34 720.2 0 34 180 38 720.2 0 38 181 30 720.2 0 30 182 64 720.2 0 64 183 42 720.2 0 42 184 40 720.2 0 40 185 50 720.2 0 50 186 41 720.2 0 41 187 28 720.2 0 28 188 32 720.2 0 32 189 32 720.2 0 32 190 47 720.2 0 47 191 108 720.2 0 108 192 38 720.2 0 38 193 26 720.2 0 26 194 45 720.2 0 45 195 30 720.2 0 30 196 45 720.2 0 45 197 28 720.2 0 28 198 15 720.2 0 15 199 23 720.2 0 23 200 29 720.2 0 29 201 34 720.2 0 34 202 28 720.2 0 28 203 30 720.2 0 30 204 30 720.2 0 30 205 19 720.2 0 19 206 25 720.2 0 25 207 73 720.2 0 73 208 100 720.2 0 100 209 172 720.2 0 172 210 102 720.2 0 102 211 294 720.2 0 294 212 57 720.2 0 57 213 53 720.2 0 53 214 184 720.2 0 184 215 122 720.2 0 122 216 196 720.2 0 196 217 45 720.2 0 45 218 29 720.2 0 29 219 44 720.2 0 44 220 42 720.2 0 42 221 56 720.2 0 56 222 58 720.2 0 58 223 37 720.2 0 37 224 54 720.2 0 54 225 117 720.2 0 117 226 68 720.2 0 68 227 51 720.2 0 51 228 59 720.2 0 59 229 56 720.2 0 56 230 123 720.2 0 123 231 49 720.2 0 49 232 53 720.2 0 53 233 86 720.2 0 86 234 115 720.2 0 115 235 152 720.2 0 152 236 67 720.2 0 67 237 38 720.2 0 38 238 38 720.2 0 38 239 28 720.2 0 28 240 40 720.2 0 40 241 36 720.2 0 36 242 51 720.2 0 51 243 60 720.2 0 60 244 56 720.2 0 56 245 50 720.2 0 50 246 47 720.2 0 47 247 27 720.2 0 27 248 38 720.2 0 38 249 34 720.2 0 34 250 24 720.2 0 24 251 32 720.2 0 32 252 41 720.2 0 41 253 48 720.2 0 48 254 65 720.2 0 65 255 47 720.2 0 47 256 51 720.2 0 51 257 43 720.2 0 43 258 60 720.2 0 60 259 54 720.2 0 54 260 90 720.2 0 90 261 81 720.2 0 81 262 56 720.2 0 56 263 41 720.2 0 41 264 59 720.2 0 59 265 49 720.2 0 49 266 63 720.2 0 63 267 59 720.2 0 59 268 93 720.2 0 93 269 285 720.2 0 285 270 246 720.2 0 246 271 331 720.2 0 331 272 98 720.2 0 98 273 125 720.2 0 125 274 107 720.2 0 107 275 104 720.2 0 104 276 105 720.2 0 105 277 66 720.2 0 66 278 50 720.2 0 50 279 75 720.2 0 75 280 96 720.2 0 96 281 131 720.2 0 131 282 101 720.2 0 101 283 78 720.2 0 78 284 63 720.2 0 63 285 88 720.2 0 88 286 65 720.2 0 65 287 45 720.2 0 45 288 74 720.2 0 74 289 81 720.2 0 81 290 104 720.2 0 104 291 102 720.2 0 102 292 99 720.2 0 99 293 148 720.2 0 148 294 465 720.2 0 465 295 864 720.2 0 864 296 1002 720.2 0 1002 297 338 720.2 0 338 298 126 720.2 0 126 299 87 720.2 0 87 300 138 720.2 0 138 301 394 720.2 0 394 Finished in 12.19 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 161,632 Reads with adapters: 82,276 (50.9%) Reads that were too short: 82 (0.1%) Reads written (passing filters): 82,194 (50.9%) Total basepairs processed: 46,329,747 bp Total written (filtered): 22,746,864 bp (49.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 82276 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 34 0.0 3 0 0 16 18 16 757 0.0 3 0 643 70 44 17 80624 0.0 3 76446 3527 530 121 18 199 0.0 3 20 143 14 22 19 239 0.0 3 3 0 153 83 20 196 0.0 3 0 0 0 196 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 1 27 1 0.0 3 1 32 1 0.0 3 0 0 0 1 34 2 0.0 3 1 0 0 1 40 2 0.0 3 0 0 0 2 43 20 0.0 3 0 0 0 20 45 1 0.0 3 0 0 0 1 46 5 0.0 3 0 0 0 5 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 52 7 0.0 3 0 0 0 7 53 7 0.0 3 0 0 0 7 54 2 0.0 3 0 0 1 1 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 14 0.0 3 0 0 0 14 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 68 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 89 1 0.0 3 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 125 7 0.0 3 0 0 0 7 127 1 0.0 3 0 0 0 1 128 6 0.0 3 0 0 0 6 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 1 133 2 0.0 3 0 0 0 2 135 6 0.0 3 0 0 0 6 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 5 0.0 3 0 0 0 5 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 4 0.0 3 0 0 0 4 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 1 1 156 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 167 5 0.0 3 0 0 0 5 175 1 0.0 3 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 244 3 0.0 3 0 0 0 3 247 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 256 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 2 0.0 3 0 0 0 2 281 8 0.0 3 0 0 0 8 282 4 0.0 3 0 0 0 4 283 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.76 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 82,194 Reads with adapters: 289 (0.4%) Reads written (passing filters): 82,194 (100.0%) Total basepairs processed: 22,746,864 bp Total written (filtered): 22,745,956 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 289 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.6% C: 46.4% G: 21.1% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 263 1284.3 0 263 4 15 321.1 0 15 5 8 80.3 0 8 6 2 20.1 0 2 7 1 5.0 0 1