This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 142.27 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 168,582 (5.7%) Reads that were too short: 5,907 (0.2%) Reads written (passing filters): 162,675 (5.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 46,961,747 bp (5.3%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 168582 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 720.2 0 1019 7 70414 720.2 0 70414 8 83980 720.2 0 83980 9 58 720.2 0 58 10 10 720.2 0 10 11 8 720.2 0 8 12 11 720.2 0 11 13 9 720.2 0 9 14 12 720.2 0 12 15 17 720.2 0 17 16 14 720.2 0 14 17 14 720.2 0 14 18 32 720.2 0 32 19 15 720.2 0 15 20 47 720.2 0 47 21 10 720.2 0 10 22 9 720.2 0 9 23 14 720.2 0 14 24 11 720.2 0 11 25 13 720.2 0 13 26 14 720.2 0 14 27 16 720.2 0 16 28 9 720.2 0 9 29 13 720.2 0 13 30 17 720.2 0 17 31 14 720.2 0 14 32 19 720.2 0 19 33 16 720.2 0 16 34 16 720.2 0 16 35 22 720.2 0 22 36 73 720.2 0 73 37 130 720.2 0 130 38 155 720.2 0 155 39 19 720.2 0 19 40 16 720.2 0 16 41 12 720.2 0 12 42 17 720.2 0 17 43 27 720.2 0 27 44 166 720.2 0 166 45 192 720.2 0 192 46 18 720.2 0 18 47 26 720.2 0 26 48 15 720.2 0 15 49 32 720.2 0 32 50 15 720.2 0 15 51 14 720.2 0 14 52 29 720.2 0 29 53 20 720.2 0 20 54 30 720.2 0 30 55 27 720.2 0 27 56 53 720.2 0 53 57 24 720.2 0 24 58 32 720.2 0 32 59 31 720.2 0 31 60 9 720.2 0 9 61 17 720.2 0 17 62 11 720.2 0 11 63 16 720.2 0 16 64 17 720.2 0 17 65 22 720.2 0 22 66 15 720.2 0 15 67 10 720.2 0 10 68 14 720.2 0 14 69 8 720.2 0 8 70 11 720.2 0 11 71 29 720.2 0 29 72 18 720.2 0 18 73 49 720.2 0 49 74 15 720.2 0 15 75 15 720.2 0 15 76 18 720.2 0 18 77 20 720.2 0 20 78 19 720.2 0 19 79 19 720.2 0 19 80 20 720.2 0 20 81 12 720.2 0 12 82 12 720.2 0 12 83 12 720.2 0 12 84 22 720.2 0 22 85 20 720.2 0 20 86 12 720.2 0 12 87 23 720.2 0 23 88 28 720.2 0 28 89 23 720.2 0 23 90 21 720.2 0 21 91 21 720.2 0 21 92 18 720.2 0 18 93 21 720.2 0 21 94 28 720.2 0 28 95 25 720.2 0 25 96 40 720.2 0 40 97 19 720.2 0 19 98 10 720.2 0 10 99 16 720.2 0 16 100 7 720.2 0 7 101 17 720.2 0 17 102 16 720.2 0 16 103 14 720.2 0 14 104 23 720.2 0 23 105 25 720.2 0 25 106 14 720.2 0 14 107 17 720.2 0 17 108 25 720.2 0 25 109 11 720.2 0 11 110 8 720.2 0 8 111 22 720.2 0 22 112 14 720.2 0 14 113 21 720.2 0 21 114 19 720.2 0 19 115 34 720.2 0 34 116 21 720.2 0 21 117 16 720.2 0 16 118 24 720.2 0 24 119 11 720.2 0 11 120 30 720.2 0 30 121 17 720.2 0 17 122 36 720.2 0 36 123 21 720.2 0 21 124 25 720.2 0 25 125 26 720.2 0 26 126 66 720.2 0 66 127 51 720.2 0 51 128 147 720.2 0 147 129 53 720.2 0 53 130 44 720.2 0 44 131 94 720.2 0 94 132 250 720.2 0 250 133 107 720.2 0 107 134 64 720.2 0 64 135 409 720.2 0 409 136 166 720.2 0 166 137 272 720.2 0 272 138 26 720.2 0 26 139 88 720.2 0 88 140 80 720.2 0 80 141 80 720.2 0 80 142 93 720.2 0 93 143 100 720.2 0 100 144 118 720.2 0 118 145 108 720.2 0 108 146 22 720.2 0 22 147 55 720.2 0 55 148 237 720.2 0 237 149 207 720.2 0 207 150 230 720.2 0 230 151 24 720.2 0 24 152 19 720.2 0 19 153 23 720.2 0 23 154 22 720.2 0 22 155 28 720.2 0 28 156 26 720.2 0 26 157 13 720.2 0 13 158 16 720.2 0 16 159 20 720.2 0 20 160 33 720.2 0 33 161 24 720.2 0 24 162 27 720.2 0 27 163 20 720.2 0 20 164 17 720.2 0 17 165 23 720.2 0 23 166 25 720.2 0 25 167 24 720.2 0 24 168 22 720.2 0 22 169 28 720.2 0 28 170 22 720.2 0 22 171 18 720.2 0 18 172 14 720.2 0 14 173 17 720.2 0 17 174 22 720.2 0 22 175 23 720.2 0 23 176 18 720.2 0 18 177 17 720.2 0 17 178 20 720.2 0 20 179 25 720.2 0 25 180 19 720.2 0 19 181 18 720.2 0 18 182 29 720.2 0 29 183 19 720.2 0 19 184 18 720.2 0 18 185 19 720.2 0 19 186 30 720.2 0 30 187 16 720.2 0 16 188 15 720.2 0 15 189 16 720.2 0 16 190 35 720.2 0 35 191 108 720.2 0 108 192 18 720.2 0 18 193 21 720.2 0 21 194 19 720.2 0 19 195 16 720.2 0 16 196 31 720.2 0 31 197 23 720.2 0 23 198 18 720.2 0 18 199 21 720.2 0 21 200 20 720.2 0 20 201 34 720.2 0 34 202 23 720.2 0 23 203 18 720.2 0 18 204 21 720.2 0 21 205 13 720.2 0 13 206 23 720.2 0 23 207 33 720.2 0 33 208 57 720.2 0 57 209 66 720.2 0 66 210 47 720.2 0 47 211 203 720.2 0 203 212 48 720.2 0 48 213 37 720.2 0 37 214 88 720.2 0 88 215 41 720.2 0 41 216 99 720.2 0 99 217 20 720.2 0 20 218 10 720.2 0 10 219 11 720.2 0 11 220 21 720.2 0 21 221 24 720.2 0 24 222 40 720.2 0 40 223 12 720.2 0 12 224 25 720.2 0 25 225 97 720.2 0 97 226 34 720.2 0 34 227 28 720.2 0 28 228 26 720.2 0 26 229 51 720.2 0 51 230 57 720.2 0 57 231 29 720.2 0 29 232 22 720.2 0 22 233 37 720.2 0 37 234 66 720.2 0 66 235 67 720.2 0 67 236 33 720.2 0 33 237 24 720.2 0 24 238 28 720.2 0 28 239 17 720.2 0 17 240 28 720.2 0 28 241 20 720.2 0 20 242 22 720.2 0 22 243 29 720.2 0 29 244 23 720.2 0 23 245 31 720.2 0 31 246 23 720.2 0 23 247 19 720.2 0 19 248 21 720.2 0 21 249 19 720.2 0 19 250 19 720.2 0 19 251 19 720.2 0 19 252 30 720.2 0 30 253 28 720.2 0 28 254 44 720.2 0 44 255 27 720.2 0 27 256 33 720.2 0 33 257 29 720.2 0 29 258 41 720.2 0 41 259 30 720.2 0 30 260 34 720.2 0 34 261 50 720.2 0 50 262 28 720.2 0 28 263 29 720.2 0 29 264 30 720.2 0 30 265 26 720.2 0 26 266 60 720.2 0 60 267 41 720.2 0 41 268 44 720.2 0 44 269 116 720.2 0 116 270 129 720.2 0 129 271 137 720.2 0 137 272 40 720.2 0 40 273 43 720.2 0 43 274 44 720.2 0 44 275 38 720.2 0 38 276 48 720.2 0 48 277 35 720.2 0 35 278 34 720.2 0 34 279 73 720.2 0 73 280 63 720.2 0 63 281 76 720.2 0 76 282 44 720.2 0 44 283 53 720.2 0 53 284 41 720.2 0 41 285 42 720.2 0 42 286 59 720.2 0 59 287 42 720.2 0 42 288 41 720.2 0 41 289 27 720.2 0 27 290 46 720.2 0 46 291 58 720.2 0 58 292 58 720.2 0 58 293 74 720.2 0 74 294 243 720.2 0 243 295 512 720.2 0 512 296 540 720.2 0 540 297 187 720.2 0 187 298 91 720.2 0 91 299 45 720.2 0 45 300 78 720.2 0 78 301 277 720.2 0 277 Finished in 13.20 s (81 us/read; 0.74 M reads/minute). === Summary === Total reads processed: 162,675 Reads with adapters: 88,171 (54.2%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 88,154 (54.2%) Total basepairs processed: 46,961,747 bp Total written (filtered): 23,835,284 bp (50.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 88171 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 25 0.0 3 0 0 24 0 1 19 877 0.0 3 0 849 21 4 3 20 84775 0.0 4 83337 1384 49 2 3 21 205 0.0 4 36 166 1 2 22 11 0.0 4 0 0 9 2 23 49 0.0 4 2 0 0 43 4 24 2 0.0 4 1 0 0 0 1 25 3 0.0 4 3 28 1 0.0 4 1 30 1 0.0 4 1 36 1 0.0 4 1 68 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 1 91 20 0.0 4 0 0 0 0 20 92 5 0.0 4 0 0 0 0 5 98 15 0.0 4 0 0 0 0 15 112 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 23 0.0 4 0 0 0 0 23 127 2126 0.0 4 0 0 0 0 2126 128 4 0.0 4 0 0 0 0 4 130 5 0.0 4 0 0 0 0 5 140 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 1 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.22 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 88,154 Reads with adapters: 6,763 (7.7%) Reads written (passing filters): 88,154 (100.0%) Total basepairs processed: 23,835,284 bp Total written (filtered): 23,760,691 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6763 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 2.9% G: 94.5% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 43 1377.4 0 43 4 8 344.4 0 8 5 1 86.1 0 1 6 2 21.5 0 2 8 2 1.3 0 2 9 151 0.3 0 6 145 10 1438 0.1 1 50 1388 11 5084 0.0 1 23 5061 12 13 0.0 1 0 13 13 4 0.0 1 0 4 14 3 0.0 1 0 3 15 1 0.0 1 0 1 71 1 0.0 1 0 1 100 1 0.0 1 1 151 1 0.0 1 0 1 158 1 0.0 1 1 184 1 0.0 1 1 190 1 0.0 1 0 1 209 2 0.0 1 1 1 223 1 0.0 1 0 1 224 2 0.0 1 2 259 1 0.0 1 0 1 269 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.