This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 131.08 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 176,830 (6.0%) Reads that were too short: 12,748 (0.4%) Reads written (passing filters): 164,082 (5.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 45,627,932 bp (5.1%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 176830 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3560 720.2 0 3560 7 61841 720.2 0 61841 8 1138 720.2 0 1138 9 75129 720.2 0 75129 10 70 720.2 0 70 11 38 720.2 0 38 12 30 720.2 0 30 13 51 720.2 0 51 14 64 720.2 0 64 15 41 720.2 0 41 16 57 720.2 0 57 17 65 720.2 0 65 18 34 720.2 0 34 19 52 720.2 0 52 20 38 720.2 0 38 21 43 720.2 0 43 22 41 720.2 0 41 23 51 720.2 0 51 24 41 720.2 0 41 25 28 720.2 0 28 26 43 720.2 0 43 27 43 720.2 0 43 28 42 720.2 0 42 29 37 720.2 0 37 30 52 720.2 0 52 31 39 720.2 0 39 32 28 720.2 0 28 33 36 720.2 0 36 34 33 720.2 0 33 35 49 720.2 0 49 36 30 720.2 0 30 37 36 720.2 0 36 38 41 720.2 0 41 39 42 720.2 0 42 40 37 720.2 0 37 41 51 720.2 0 51 42 200 720.2 0 200 43 205 720.2 0 205 44 180 720.2 0 180 45 37 720.2 0 37 46 30 720.2 0 30 47 36 720.2 0 36 48 52 720.2 0 52 49 39 720.2 0 39 50 60 720.2 0 60 51 40 720.2 0 40 52 39 720.2 0 39 53 38 720.2 0 38 54 41 720.2 0 41 55 49 720.2 0 49 56 47 720.2 0 47 57 31 720.2 0 31 58 49 720.2 0 49 59 39 720.2 0 39 60 28 720.2 0 28 61 33 720.2 0 33 62 37 720.2 0 37 63 31 720.2 0 31 64 35 720.2 0 35 65 69 720.2 0 69 66 63 720.2 0 63 67 39 720.2 0 39 68 45 720.2 0 45 69 41 720.2 0 41 70 29 720.2 0 29 71 33 720.2 0 33 72 32 720.2 0 32 73 49 720.2 0 49 74 67 720.2 0 67 75 95 720.2 0 95 76 70 720.2 0 70 77 72 720.2 0 72 78 29 720.2 0 29 79 60 720.2 0 60 80 103 720.2 0 103 81 34 720.2 0 34 82 26 720.2 0 26 83 31 720.2 0 31 84 36 720.2 0 36 85 30 720.2 0 30 86 47 720.2 0 47 87 51 720.2 0 51 88 45 720.2 0 45 89 62 720.2 0 62 90 73 720.2 0 73 91 80 720.2 0 80 92 894 720.2 0 894 93 937 720.2 0 937 94 2478 720.2 0 2478 95 57 720.2 0 57 96 35 720.2 0 35 97 39 720.2 0 39 98 33 720.2 0 33 99 33 720.2 0 33 100 49 720.2 0 49 101 42 720.2 0 42 102 23 720.2 0 23 103 22 720.2 0 22 104 35 720.2 0 35 105 51 720.2 0 51 106 36 720.2 0 36 107 41 720.2 0 41 108 59 720.2 0 59 109 45 720.2 0 45 110 77 720.2 0 77 111 47 720.2 0 47 112 90 720.2 0 90 113 170 720.2 0 170 114 48 720.2 0 48 115 40 720.2 0 40 116 56 720.2 0 56 117 63 720.2 0 63 118 63 720.2 0 63 119 87 720.2 0 87 120 91 720.2 0 91 121 94 720.2 0 94 122 95 720.2 0 95 123 1128 720.2 0 1128 124 2324 720.2 0 2324 125 848 720.2 0 848 126 332 720.2 0 332 127 50 720.2 0 50 128 75 720.2 0 75 129 330 720.2 0 330 130 429 720.2 0 429 131 1059 720.2 0 1059 132 570 720.2 0 570 133 55 720.2 0 55 134 47 720.2 0 47 135 22 720.2 0 22 136 30 720.2 0 30 137 35 720.2 0 35 138 42 720.2 0 42 139 36 720.2 0 36 140 55 720.2 0 55 141 27 720.2 0 27 142 74 720.2 0 74 143 59 720.2 0 59 144 29 720.2 0 29 145 38 720.2 0 38 146 64 720.2 0 64 147 58 720.2 0 58 148 56 720.2 0 56 149 32 720.2 0 32 150 20 720.2 0 20 151 39 720.2 0 39 152 47 720.2 0 47 153 36 720.2 0 36 154 36 720.2 0 36 155 31 720.2 0 31 156 18 720.2 0 18 157 31 720.2 0 31 158 54 720.2 0 54 159 35 720.2 0 35 160 35 720.2 0 35 161 31 720.2 0 31 162 38 720.2 0 38 163 35 720.2 0 35 164 35 720.2 0 35 165 32 720.2 0 32 166 36 720.2 0 36 167 80 720.2 0 80 168 41 720.2 0 41 169 83 720.2 0 83 170 30 720.2 0 30 171 25 720.2 0 25 172 36 720.2 0 36 173 79 720.2 0 79 174 84 720.2 0 84 175 52 720.2 0 52 176 93 720.2 0 93 177 65 720.2 0 65 178 74 720.2 0 74 179 181 720.2 0 181 180 190 720.2 0 190 181 119 720.2 0 119 182 58 720.2 0 58 183 45 720.2 0 45 184 53 720.2 0 53 185 49 720.2 0 49 186 118 720.2 0 118 187 35 720.2 0 35 188 70 720.2 0 70 189 31 720.2 0 31 190 44 720.2 0 44 191 48 720.2 0 48 192 69 720.2 0 69 193 75 720.2 0 75 194 76 720.2 0 76 195 134 720.2 0 134 196 95 720.2 0 95 197 110 720.2 0 110 198 293 720.2 0 293 199 387 720.2 0 387 200 478 720.2 0 478 201 321 720.2 0 321 202 193 720.2 0 193 203 74 720.2 0 74 204 66 720.2 0 66 205 103 720.2 0 103 206 94 720.2 0 94 207 178 720.2 0 178 208 104 720.2 0 104 209 68 720.2 0 68 210 58 720.2 0 58 211 60 720.2 0 60 212 54 720.2 0 54 213 35 720.2 0 35 214 39 720.2 0 39 215 74 720.2 0 74 216 78 720.2 0 78 217 67 720.2 0 67 218 88 720.2 0 88 219 195 720.2 0 195 220 240 720.2 0 240 221 313 720.2 0 313 222 342 720.2 0 342 223 148 720.2 0 148 224 384 720.2 0 384 225 84 720.2 0 84 226 51 720.2 0 51 227 66 720.2 0 66 228 97 720.2 0 97 229 100 720.2 0 100 230 75 720.2 0 75 231 44 720.2 0 44 232 50 720.2 0 50 233 37 720.2 0 37 234 44 720.2 0 44 235 45 720.2 0 45 236 48 720.2 0 48 237 64 720.2 0 64 238 214 720.2 0 214 239 195 720.2 0 195 240 1020 720.2 0 1020 241 718 720.2 0 718 242 1605 720.2 0 1605 243 63 720.2 0 63 244 52 720.2 0 52 245 39 720.2 0 39 246 49 720.2 0 49 247 94 720.2 0 94 248 80 720.2 0 80 249 71 720.2 0 71 250 129 720.2 0 129 251 240 720.2 0 240 252 189 720.2 0 189 253 113 720.2 0 113 254 34 720.2 0 34 255 30 720.2 0 30 256 67 720.2 0 67 257 69 720.2 0 69 258 54 720.2 0 54 259 34 720.2 0 34 260 54 720.2 0 54 261 59 720.2 0 59 262 86 720.2 0 86 263 211 720.2 0 211 264 46 720.2 0 46 265 41 720.2 0 41 266 36 720.2 0 36 267 47 720.2 0 47 268 41 720.2 0 41 269 41 720.2 0 41 270 64 720.2 0 64 271 73 720.2 0 73 272 56 720.2 0 56 273 67 720.2 0 67 274 43 720.2 0 43 275 32 720.2 0 32 276 43 720.2 0 43 277 72 720.2 0 72 278 51 720.2 0 51 279 75 720.2 0 75 280 53 720.2 0 53 281 56 720.2 0 56 282 77 720.2 0 77 283 105 720.2 0 105 284 130 720.2 0 130 285 91 720.2 0 91 286 81 720.2 0 81 287 122 720.2 0 122 288 177 720.2 0 177 289 199 720.2 0 199 290 269 720.2 0 269 291 186 720.2 0 186 292 161 720.2 0 161 293 81 720.2 0 81 294 56 720.2 0 56 295 40 720.2 0 40 296 59 720.2 0 59 297 64 720.2 0 64 298 54 720.2 0 54 299 148 720.2 0 148 300 194 720.2 0 194 301 88 720.2 0 88 Finished in 11.51 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 164,082 Reads with adapters: 77,535 (47.3%) Reads that were too short: 82 (0.0%) Reads written (passing filters): 77,453 (47.2%) Total basepairs processed: 45,627,932 bp Total written (filtered): 21,288,492 bp (46.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 77535 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 35 0.0 3 0 0 24 11 16 606 0.0 3 0 524 59 23 17 75626 0.0 3 71091 3840 576 119 18 191 0.0 3 14 112 15 50 19 156 0.0 3 3 8 81 64 20 742 0.0 3 550 28 8 156 21 2 0.0 3 0 1 0 1 22 1 0.0 3 0 0 1 25 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 30 2 0.0 3 0 1 0 1 42 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 3 0.0 3 1 0 0 2 47 7 0.0 3 0 0 0 7 48 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 1 59 1 0.0 3 0 0 0 1 62 5 0.0 3 0 0 0 5 63 6 0.0 3 0 0 0 6 70 2 0.0 3 0 0 0 2 71 3 0.0 3 0 0 0 3 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 88 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 4 0.0 3 0 0 0 4 97 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 7 0.0 3 0 0 1 6 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 149 6 0.0 3 0 0 1 5 150 3 0.0 3 0 0 0 3 152 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 176 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 8 0.0 3 0 0 1 7 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 206 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 213 1 0.0 3 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 282 4 0.0 3 0 0 0 4 286 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.34 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 77,453 Reads with adapters: 1,291 (1.7%) Reads written (passing filters): 77,453 (100.0%) Total basepairs processed: 21,288,492 bp Total written (filtered): 21,284,339 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1291 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.0% C: 10.3% G: 47.2% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1051 1210.2 0 1051 4 231 302.6 0 231 5 2 75.6 0 2 9 6 0.3 0 0 6 12 1 0.0 1 0 1