This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 138.54 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 531,609 (18.0%) Reads that were too short: 78,104 (2.6%) Reads written (passing filters): 453,505 (15.4%) Total basepairs processed: 887,907,860 bp Total written (filtered): 126,542,948 bp (14.3%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 531609 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3654 720.2 0 3654 7 68752 720.2 0 68752 8 3729 720.2 0 3729 9 53793 720.2 0 53793 10 7504 720.2 0 7504 11 265794 720.2 0 265794 12 431 720.2 0 431 13 83 720.2 0 83 14 60 720.2 0 60 15 70 720.2 0 70 16 53 720.2 0 53 17 35 720.2 0 35 18 48 720.2 0 48 19 64 720.2 0 64 20 68 720.2 0 68 21 47 720.2 0 47 22 61 720.2 0 61 23 67 720.2 0 67 24 61 720.2 0 61 25 58 720.2 0 58 26 86 720.2 0 86 27 69 720.2 0 69 28 153 720.2 0 153 29 177 720.2 0 177 30 84 720.2 0 84 31 61 720.2 0 61 32 71 720.2 0 71 33 57 720.2 0 57 34 70 720.2 0 70 35 54 720.2 0 54 36 55 720.2 0 55 37 75 720.2 0 75 38 72 720.2 0 72 39 93 720.2 0 93 40 131 720.2 0 131 41 101 720.2 0 101 42 68 720.2 0 68 43 56 720.2 0 56 44 83 720.2 0 83 45 72 720.2 0 72 46 62 720.2 0 62 47 62 720.2 0 62 48 100 720.2 0 100 49 203 720.2 0 203 50 82 720.2 0 82 51 52 720.2 0 52 52 64 720.2 0 64 53 64 720.2 0 64 54 88 720.2 0 88 55 76 720.2 0 76 56 87 720.2 0 87 57 76 720.2 0 76 58 56 720.2 0 56 59 67 720.2 0 67 60 65 720.2 0 65 61 79 720.2 0 79 62 61 720.2 0 61 63 60 720.2 0 60 64 77 720.2 0 77 65 65 720.2 0 65 66 49 720.2 0 49 67 78 720.2 0 78 68 56 720.2 0 56 69 75 720.2 0 75 70 198 720.2 0 198 71 161 720.2 0 161 72 78 720.2 0 78 73 57 720.2 0 57 74 49 720.2 0 49 75 69 720.2 0 69 76 88 720.2 0 88 77 67 720.2 0 67 78 106 720.2 0 106 79 446 720.2 0 446 80 211 720.2 0 211 81 278 720.2 0 278 82 136 720.2 0 136 83 1079 720.2 0 1079 84 1187 720.2 0 1187 85 338 720.2 0 338 86 83 720.2 0 83 87 56 720.2 0 56 88 59 720.2 0 59 89 95 720.2 0 95 90 81 720.2 0 81 91 54 720.2 0 54 92 58 720.2 0 58 93 58 720.2 0 58 94 63 720.2 0 63 95 66 720.2 0 66 96 88 720.2 0 88 97 170 720.2 0 170 98 584 720.2 0 584 99 167 720.2 0 167 100 185 720.2 0 185 101 176 720.2 0 176 102 113 720.2 0 113 103 111 720.2 0 111 104 76 720.2 0 76 105 99 720.2 0 99 106 135 720.2 0 135 107 128 720.2 0 128 108 127 720.2 0 127 109 125 720.2 0 125 110 136 720.2 0 136 111 177 720.2 0 177 112 371 720.2 0 371 113 2267 720.2 0 2267 114 6465 720.2 0 6465 115 3482 720.2 0 3482 116 200 720.2 0 200 117 75 720.2 0 75 118 73 720.2 0 73 119 110 720.2 0 110 120 99 720.2 0 99 121 78 720.2 0 78 122 113 720.2 0 113 123 109 720.2 0 109 124 155 720.2 0 155 125 106 720.2 0 106 126 174 720.2 0 174 127 176 720.2 0 176 128 189 720.2 0 189 129 2067 720.2 0 2067 130 3071 720.2 0 3071 131 5764 720.2 0 5764 132 177 720.2 0 177 133 178 720.2 0 178 134 343 720.2 0 343 135 110 720.2 0 110 136 106 720.2 0 106 137 322 720.2 0 322 138 177 720.2 0 177 139 161 720.2 0 161 140 155 720.2 0 155 141 206 720.2 0 206 142 120 720.2 0 120 143 260 720.2 0 260 144 190 720.2 0 190 145 102 720.2 0 102 146 131 720.2 0 131 147 82 720.2 0 82 148 91 720.2 0 91 149 85 720.2 0 85 150 102 720.2 0 102 151 103 720.2 0 103 152 116 720.2 0 116 153 116 720.2 0 116 154 500 720.2 0 500 155 2030 720.2 0 2030 156 1346 720.2 0 1346 157 757 720.2 0 757 158 772 720.2 0 772 159 53 720.2 0 53 160 70 720.2 0 70 161 56 720.2 0 56 162 60 720.2 0 60 163 111 720.2 0 111 164 74 720.2 0 74 165 55 720.2 0 55 166 71 720.2 0 71 167 339 720.2 0 339 168 132 720.2 0 132 169 136 720.2 0 136 170 55 720.2 0 55 171 167 720.2 0 167 172 66 720.2 0 66 173 48 720.2 0 48 174 53 720.2 0 53 175 40 720.2 0 40 176 53 720.2 0 53 177 57 720.2 0 57 178 85 720.2 0 85 179 57 720.2 0 57 180 56 720.2 0 56 181 35 720.2 0 35 182 42 720.2 0 42 183 46 720.2 0 46 184 69 720.2 0 69 185 56 720.2 0 56 186 72 720.2 0 72 187 58 720.2 0 58 188 54 720.2 0 54 189 77 720.2 0 77 190 141 720.2 0 141 191 122 720.2 0 122 192 90 720.2 0 90 193 51 720.2 0 51 194 55 720.2 0 55 195 60 720.2 0 60 196 90 720.2 0 90 197 100 720.2 0 100 198 77 720.2 0 77 199 62 720.2 0 62 200 445 720.2 0 445 201 85 720.2 0 85 202 313 720.2 0 313 203 85 720.2 0 85 204 90 720.2 0 90 205 70 720.2 0 70 206 56 720.2 0 56 207 51 720.2 0 51 208 54 720.2 0 54 209 58 720.2 0 58 210 76 720.2 0 76 211 173 720.2 0 173 212 266 720.2 0 266 213 305 720.2 0 305 214 89 720.2 0 89 215 89 720.2 0 89 216 99 720.2 0 99 217 72 720.2 0 72 218 71 720.2 0 71 219 48 720.2 0 48 220 57 720.2 0 57 221 85 720.2 0 85 222 62 720.2 0 62 223 57 720.2 0 57 224 89 720.2 0 89 225 77 720.2 0 77 226 61 720.2 0 61 227 76 720.2 0 76 228 238 720.2 0 238 229 369 720.2 0 369 230 474 720.2 0 474 231 180 720.2 0 180 232 231 720.2 0 231 233 246 720.2 0 246 234 149 720.2 0 149 235 116 720.2 0 116 236 166 720.2 0 166 237 160 720.2 0 160 238 97 720.2 0 97 239 73 720.2 0 73 240 84 720.2 0 84 241 65 720.2 0 65 242 85 720.2 0 85 243 81 720.2 0 81 244 109 720.2 0 109 245 78 720.2 0 78 246 124 720.2 0 124 247 234 720.2 0 234 248 937 720.2 0 937 249 177 720.2 0 177 250 84 720.2 0 84 251 88 720.2 0 88 252 54 720.2 0 54 253 85 720.2 0 85 254 139 720.2 0 139 255 157 720.2 0 157 256 171 720.2 0 171 257 316 720.2 0 316 258 191 720.2 0 191 259 112 720.2 0 112 260 66 720.2 0 66 261 84 720.2 0 84 262 68 720.2 0 68 263 101 720.2 0 101 264 90 720.2 0 90 265 169 720.2 0 169 266 71 720.2 0 71 267 73 720.2 0 73 268 165 720.2 0 165 269 395 720.2 0 395 270 520 720.2 0 520 271 309 720.2 0 309 272 151 720.2 0 151 273 140 720.2 0 140 274 175 720.2 0 175 275 84 720.2 0 84 276 125 720.2 0 125 277 108 720.2 0 108 278 93 720.2 0 93 279 88 720.2 0 88 280 105 720.2 0 105 281 207 720.2 0 207 282 187 720.2 0 187 283 231 720.2 0 231 284 270 720.2 0 270 285 334 720.2 0 334 286 162 720.2 0 162 287 151 720.2 0 151 288 269 720.2 0 269 289 205 720.2 0 205 290 199 720.2 0 199 291 261 720.2 0 261 292 304 720.2 0 304 293 358 720.2 0 358 294 869 720.2 0 869 295 1613 720.2 0 1613 296 4252 720.2 0 4252 297 2590 720.2 0 2590 298 15946 720.2 0 15946 299 30490 720.2 0 30490 300 7332 720.2 0 7332 301 195 720.2 0 195 Finished in 28.80 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 453,505 Reads with adapters: 66,710 (14.7%) Reads that were too short: 495 (0.1%) Reads written (passing filters): 66,215 (14.6%) Total basepairs processed: 126,542,948 bp Total written (filtered): 18,287,281 bp (14.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 66710 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 29 0.0 3 0 0 16 13 16 444 0.0 3 0 356 57 31 17 64801 0.0 3 60971 3085 600 145 18 173 0.0 3 16 116 10 31 19 170 0.0 3 0 0 93 77 20 233 0.0 3 1 0 1 231 23 10 0.0 3 0 0 0 10 24 1 0.0 3 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 169 0.0 3 0 0 1 168 34 7 0.0 3 0 0 0 7 35 17 0.0 3 0 0 0 17 37 1 0.0 3 0 0 0 1 38 4 0.0 3 0 0 0 4 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 46 4 0.0 3 1 0 0 3 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 5 0.0 3 0 0 0 5 52 2 0.0 3 0 0 0 2 53 10 0.0 3 0 0 0 10 54 4 0.0 3 0 0 0 4 56 3 0.0 3 0 0 0 3 58 4 0.0 3 0 0 0 4 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 4 0.0 3 0 0 0 4 62 11 0.0 3 0 0 0 11 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 7 0.0 3 0 0 6 1 76 3 0.0 3 0 0 0 3 77 7 0.0 3 0 0 0 7 78 4 0.0 3 0 0 0 4 79 6 0.0 3 0 0 1 5 81 2 0.0 3 0 0 0 2 83 4 0.0 3 0 0 0 4 84 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 88 1 0.0 3 0 0 0 1 89 1 0.0 3 1 90 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 1 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 1 113 3 0.0 3 0 0 0 3 115 3 0.0 3 0 0 0 3 116 3 0.0 3 0 0 0 3 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 125 8 0.0 3 0 0 0 8 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 3 0.0 3 0 0 0 3 135 5 0.0 3 0 0 0 5 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 1 2 142 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 1 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 1 1 153 5 0.0 3 0 0 0 5 154 6 0.0 3 0 0 0 6 155 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 162 4 0.0 3 0 0 0 4 163 13 0.0 3 0 0 0 13 164 18 0.0 3 0 0 1 17 165 3 0.0 3 0 0 0 3 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 2 0.0 3 0 0 1 1 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 189 2 0.0 3 0 0 0 2 190 7 0.0 3 0 0 0 7 191 1 0.0 3 0 0 0 1 192 33 0.0 3 0 0 2 31 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 195 2 0.0 3 1 0 0 1 200 20 0.0 3 0 0 0 20 201 1 0.0 3 0 0 0 1 203 10 0.0 3 0 0 1 9 206 2 0.0 3 0 0 0 2 214 4 0.0 3 0 0 0 4 215 92 0.0 3 0 0 6 86 216 6 0.0 3 0 0 0 6 217 4 0.0 3 0 0 0 4 218 3 0.0 3 0 0 0 3 219 7 0.0 3 0 0 0 7 220 3 0.0 3 0 0 0 3 221 4 0.0 3 0 0 0 4 223 1 0.0 3 0 0 0 1 224 3 0.0 3 0 0 0 3 231 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 236 22 0.0 3 0 0 0 22 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 244 3 0.0 3 0 0 1 2 246 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 2 0.0 3 1 0 0 1 265 1 0.0 3 0 0 0 1 268 11 0.0 3 0 0 0 11 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 16 0.0 3 0 0 0 16 278 33 0.0 3 0 0 0 33 279 34 0.0 3 0 0 0 34 280 21 0.0 3 0 0 0 21 281 9 0.0 3 0 0 1 8 282 4 0.0 3 0 0 1 3 284 1 0.0 3 0 0 0 1 286 7 0.0 3 0 0 0 7 287 3 0.0 3 0 0 0 3 289 3 0.0 3 0 0 0 3 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.61 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 66,215 Reads with adapters: 165 (0.2%) Reads written (passing filters): 66,215 (100.0%) Total basepairs processed: 18,287,281 bp Total written (filtered): 18,286,745 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 165 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.8% C: 32.7% G: 25.5% T: 6.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 152 1034.6 0 152 4 5 258.7 0 5 5 3 64.7 0 3 6 2 16.2 0 2 9 1 0.3 0 0 1 12 2 0.0 1 0 2