This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.89 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 594,807 (20.2%) Reads that were too short: 74,517 (2.5%) Reads written (passing filters): 520,290 (17.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 146,247,120 bp (16.5%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 594807 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3810 720.2 0 3810 7 59386 720.2 0 59386 8 3158 720.2 0 3158 9 66559 720.2 0 66559 10 6249 720.2 0 6249 11 334435 720.2 0 334435 12 434 720.2 0 434 13 87 720.2 0 87 14 66 720.2 0 66 15 59 720.2 0 59 16 47 720.2 0 47 17 39 720.2 0 39 18 46 720.2 0 46 19 54 720.2 0 54 20 68 720.2 0 68 21 37 720.2 0 37 22 60 720.2 0 60 23 54 720.2 0 54 24 49 720.2 0 49 25 46 720.2 0 46 26 70 720.2 0 70 27 33 720.2 0 33 28 73 720.2 0 73 29 175 720.2 0 175 30 63 720.2 0 63 31 48 720.2 0 48 32 58 720.2 0 58 33 43 720.2 0 43 34 71 720.2 0 71 35 56 720.2 0 56 36 78 720.2 0 78 37 59 720.2 0 59 38 64 720.2 0 64 39 101 720.2 0 101 40 169 720.2 0 169 41 103 720.2 0 103 42 62 720.2 0 62 43 57 720.2 0 57 44 82 720.2 0 82 45 73 720.2 0 73 46 52 720.2 0 52 47 64 720.2 0 64 48 99 720.2 0 99 49 205 720.2 0 205 50 90 720.2 0 90 51 58 720.2 0 58 52 62 720.2 0 62 53 74 720.2 0 74 54 83 720.2 0 83 55 56 720.2 0 56 56 72 720.2 0 72 57 86 720.2 0 86 58 69 720.2 0 69 59 73 720.2 0 73 60 62 720.2 0 62 61 85 720.2 0 85 62 73 720.2 0 73 63 57 720.2 0 57 64 68 720.2 0 68 65 77 720.2 0 77 66 68 720.2 0 68 67 70 720.2 0 70 68 42 720.2 0 42 69 61 720.2 0 61 70 156 720.2 0 156 71 157 720.2 0 157 72 92 720.2 0 92 73 60 720.2 0 60 74 56 720.2 0 56 75 67 720.2 0 67 76 74 720.2 0 74 77 61 720.2 0 61 78 97 720.2 0 97 79 293 720.2 0 293 80 159 720.2 0 159 81 230 720.2 0 230 82 132 720.2 0 132 83 1254 720.2 0 1254 84 1498 720.2 0 1498 85 288 720.2 0 288 86 63 720.2 0 63 87 73 720.2 0 73 88 70 720.2 0 70 89 67 720.2 0 67 90 76 720.2 0 76 91 67 720.2 0 67 92 65 720.2 0 65 93 70 720.2 0 70 94 61 720.2 0 61 95 54 720.2 0 54 96 72 720.2 0 72 97 143 720.2 0 143 98 570 720.2 0 570 99 136 720.2 0 136 100 181 720.2 0 181 101 176 720.2 0 176 102 104 720.2 0 104 103 114 720.2 0 114 104 84 720.2 0 84 105 115 720.2 0 115 106 119 720.2 0 119 107 138 720.2 0 138 108 116 720.2 0 116 109 98 720.2 0 98 110 110 720.2 0 110 111 156 720.2 0 156 112 305 720.2 0 305 113 1853 720.2 0 1853 114 5701 720.2 0 5701 115 2996 720.2 0 2996 116 127 720.2 0 127 117 81 720.2 0 81 118 79 720.2 0 79 119 104 720.2 0 104 120 71 720.2 0 71 121 73 720.2 0 73 122 114 720.2 0 114 123 89 720.2 0 89 124 132 720.2 0 132 125 102 720.2 0 102 126 114 720.2 0 114 127 131 720.2 0 131 128 134 720.2 0 134 129 1730 720.2 0 1730 130 2634 720.2 0 2634 131 4911 720.2 0 4911 132 132 720.2 0 132 133 127 720.2 0 127 134 241 720.2 0 241 135 83 720.2 0 83 136 75 720.2 0 75 137 178 720.2 0 178 138 100 720.2 0 100 139 123 720.2 0 123 140 94 720.2 0 94 141 129 720.2 0 129 142 86 720.2 0 86 143 227 720.2 0 227 144 160 720.2 0 160 145 127 720.2 0 127 146 79 720.2 0 79 147 61 720.2 0 61 148 70 720.2 0 70 149 93 720.2 0 93 150 93 720.2 0 93 151 103 720.2 0 103 152 114 720.2 0 114 153 133 720.2 0 133 154 540 720.2 0 540 155 2557 720.2 0 2557 156 1413 720.2 0 1413 157 981 720.2 0 981 158 848 720.2 0 848 159 72 720.2 0 72 160 64 720.2 0 64 161 73 720.2 0 73 162 55 720.2 0 55 163 132 720.2 0 132 164 76 720.2 0 76 165 59 720.2 0 59 166 78 720.2 0 78 167 382 720.2 0 382 168 104 720.2 0 104 169 136 720.2 0 136 170 51 720.2 0 51 171 260 720.2 0 260 172 84 720.2 0 84 173 70 720.2 0 70 174 76 720.2 0 76 175 58 720.2 0 58 176 58 720.2 0 58 177 54 720.2 0 54 178 85 720.2 0 85 179 63 720.2 0 63 180 33 720.2 0 33 181 67 720.2 0 67 182 63 720.2 0 63 183 66 720.2 0 66 184 45 720.2 0 45 185 42 720.2 0 42 186 63 720.2 0 63 187 51 720.2 0 51 188 72 720.2 0 72 189 77 720.2 0 77 190 118 720.2 0 118 191 81 720.2 0 81 192 69 720.2 0 69 193 63 720.2 0 63 194 55 720.2 0 55 195 64 720.2 0 64 196 85 720.2 0 85 197 68 720.2 0 68 198 53 720.2 0 53 199 68 720.2 0 68 200 497 720.2 0 497 201 93 720.2 0 93 202 159 720.2 0 159 203 97 720.2 0 97 204 92 720.2 0 92 205 60 720.2 0 60 206 71 720.2 0 71 207 57 720.2 0 57 208 80 720.2 0 80 209 74 720.2 0 74 210 78 720.2 0 78 211 76 720.2 0 76 212 119 720.2 0 119 213 117 720.2 0 117 214 56 720.2 0 56 215 86 720.2 0 86 216 87 720.2 0 87 217 88 720.2 0 88 218 72 720.2 0 72 219 67 720.2 0 67 220 59 720.2 0 59 221 71 720.2 0 71 222 64 720.2 0 64 223 77 720.2 0 77 224 83 720.2 0 83 225 76 720.2 0 76 226 76 720.2 0 76 227 70 720.2 0 70 228 118 720.2 0 118 229 186 720.2 0 186 230 214 720.2 0 214 231 133 720.2 0 133 232 114 720.2 0 114 233 113 720.2 0 113 234 105 720.2 0 105 235 90 720.2 0 90 236 101 720.2 0 101 237 107 720.2 0 107 238 91 720.2 0 91 239 106 720.2 0 106 240 101 720.2 0 101 241 89 720.2 0 89 242 87 720.2 0 87 243 67 720.2 0 67 244 84 720.2 0 84 245 76 720.2 0 76 246 80 720.2 0 80 247 144 720.2 0 144 248 1058 720.2 0 1058 249 136 720.2 0 136 250 71 720.2 0 71 251 71 720.2 0 71 252 61 720.2 0 61 253 86 720.2 0 86 254 118 720.2 0 118 255 108 720.2 0 108 256 105 720.2 0 105 257 155 720.2 0 155 258 128 720.2 0 128 259 102 720.2 0 102 260 55 720.2 0 55 261 65 720.2 0 65 262 76 720.2 0 76 263 77 720.2 0 77 264 87 720.2 0 87 265 177 720.2 0 177 266 56 720.2 0 56 267 79 720.2 0 79 268 139 720.2 0 139 269 234 720.2 0 234 270 246 720.2 0 246 271 235 720.2 0 235 272 111 720.2 0 111 273 88 720.2 0 88 274 140 720.2 0 140 275 79 720.2 0 79 276 109 720.2 0 109 277 109 720.2 0 109 278 86 720.2 0 86 279 82 720.2 0 82 280 78 720.2 0 78 281 149 720.2 0 149 282 132 720.2 0 132 283 167 720.2 0 167 284 233 720.2 0 233 285 358 720.2 0 358 286 148 720.2 0 148 287 133 720.2 0 133 288 218 720.2 0 218 289 154 720.2 0 154 290 165 720.2 0 165 291 215 720.2 0 215 292 260 720.2 0 260 293 284 720.2 0 284 294 594 720.2 0 594 295 958 720.2 0 958 296 2237 720.2 0 2237 297 1544 720.2 0 1544 298 14667 720.2 0 14667 299 34131 720.2 0 34131 300 8723 720.2 0 8723 301 122 720.2 0 122 Finished in 36.59 s (70 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 520,290 Reads with adapters: 69,317 (13.3%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 69,274 (13.3%) Total basepairs processed: 146,247,120 bp Total written (filtered): 18,606,804 bp (12.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 69317 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 24 0.0 3 0 0 23 1 19 658 0.0 3 0 639 17 1 1 20 66075 0.0 4 64878 1144 49 4 21 159 0.0 4 29 118 10 2 22 12 0.0 4 1 0 7 1 3 23 63 0.0 4 1 0 0 51 11 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 31 1 0.0 4 0 0 0 1 34 1 0.0 4 1 35 1 0.0 4 1 38 1 0.0 4 1 45 1 0.0 4 1 48 1 0.0 4 1 51 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 83 1 0.0 4 1 85 1 0.0 4 1 90 1 0.0 4 0 0 1 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 1 101 2 0.0 4 1 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 121 3 0.0 4 0 0 0 0 3 125 6 0.0 4 0 0 0 0 6 126 18 0.0 4 0 0 0 0 18 127 2196 0.0 4 0 0 0 0 2196 128 3 0.0 4 0 0 0 0 3 136 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 1 2 163 2 0.0 4 0 0 0 0 2 164 2 0.0 4 0 0 0 0 2 169 24 0.0 4 0 0 0 0 24 170 9 0.0 4 0 0 0 0 9 171 1 0.0 4 0 1 175 1 0.0 4 1 178 1 0.0 4 0 0 0 1 187 1 0.0 4 0 1 198 1 0.0 4 0 1 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 1 212 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 237 6 0.0 4 0 0 0 0 6 249 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 1 268 1 0.0 4 0 0 0 0 1 272 1 0.0 4 1 281 1 0.0 4 0 1 292 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.80 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 69,274 Reads with adapters: 7,051 (10.2%) Reads written (passing filters): 69,274 (100.0%) Total basepairs processed: 18,606,804 bp Total written (filtered): 18,535,732 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7051 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.6% G: 95.4% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 21 1082.4 0 21 4 11 270.6 0 11 6 2 16.9 0 2 7 3 4.2 0 3 8 2 1.1 0 2 9 3000 0.3 0 52 2948 10 3119 0.1 1 92 3027 11 870 0.0 1 5 865 12 5 0.0 1 0 5 13 2 0.0 1 0 2 96 1 0.0 1 1 120 1 0.0 1 1 138 1 0.0 1 1 153 1 0.0 1 1 169 5 0.0 1 2 3 208 1 0.0 1 1 211 1 0.0 1 0 1 223 1 0.0 1 1 269 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.